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Karst aquifers provide a significant source of drinking water around the world; however, they are prone to bacterial contamination. We investigated the prevalence, serotypes, and antimicrobial resistance genes (ARGs) of Salmonella in urban karst groundwater and groundwater-fed surface waters. Weekly water samples (n = 443) were collected from nine groundwater sites and the Barren River in Bowling Green, KY. Additionally, cross-sectional samples were collected from 45 sites, including groundwaters and two rivers, in Tampa, FL. Salmonella was detected in 14.5% of Bowling Green samples resulting in 176 isolates representing 18 serotypes. Two (4.4%) of the Tampa samples were positive yielding eight isolates representing two serotypes. In Bowling Green, Salmonella prevalence varied by sampling site, week, month, and season, with the highest prevalence in surface water in the month of November and during summer. Salmonella Newport was the most common serotype detected. Nine of the serotypes detected in the study were among the top 20 serotypes commonly associated with human infections in the United States. Isolates were clustered with human clinical isolates, or isolates obtained from food animals, suggesting the importance of humans and agricultural practices as a source of water contamination. ARGs were detected in 12.5% (n = 176) of the Bowling Green isolates; overall, 81.8% (n = 22) of these were multidrug-resistant (resistance to ≥3 antimicrobial classes). Notably, the four Salmonella Agona isolates were resistant to four antimicrobial classes, mercury, and quaternary ammonium compounds. Seasonality and the widespread occurrence of Salmonella both in the groundwater and groundwater-fed surface waters is a potential risk to public health., (© 2023 The Authors. Journal of Environmental Quality published by Wiley Periodicals LLC on behalf of American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)

The Annual P Loss Estimator (APLE) is a spreadsheet-based model developed for predicting annual field-scale P loss in surface runoff and changes in soil test P. This empirically based model was designed for use by those without significant modeling experience. However, a significant limitation with the model is that it does not calculate runoff. Moreover, APLE is deterministic and thus predicts a single value for a given set of inputs, thereby ignoring any uncertainties associated with model inputs. Here, we describe modifications to APLE that allow users to estimate runoff using the Curve Number method. Using Monte Carlo simulations, the updated version of APLE also provides users the ability to account for model input uncertainties in estimating model prediction errors. We provide examples of using the revised version of APLE (ver. 3.0) for calculating P loss from two fields in Mississippi over a 4-yr period and calculating the change in Mehlich-3 P concentrations over a 9-yr period at three locations in Maryland following cessation of P application. Both examples demonstrate that incorporating estimates of uncertainties in both measured data and model predictions provides modelers with a more realistic understanding of the model's performance., (Published 2022. This article is a U.S. Government work and is in the public domain in the USA.)

In this study we conducted a sensitivity and uncertainty analysis using the Annual P Loss Estimator (APLE) model focusing on model predictions of soil test phosphorus (STP). We calculated and evaluated the sensitivity coefficients of predicted STP and changes in STP using 1- and 10-yr simulations with and without P application. We also compared two methods for estimating prediction uncertainties: first-order variance approximation (FOVA) and Monte Carlo simulation (MCS). Finally, we compared uncertainties in APLE-predicted STP with uncertainties in measured STP collected from multiple sites in Maryland under different manuring and cropping treatments. Results from our sensitivity analysis showed that predicted STP and changes in STP for 1-yr simulations without P inputs were most sensitive to initial STP, whereas model STP predictions were most sensitive to manure and fertilizer application rates when sensitivity analyses included P inputs. For the 10-yr simulations without P application inputs, the range in sensitivity coefficients for crop uptake and precipitation were much greater than for the 1-yr simulations. Prediction uncertainties from FOVA were comparable to those from MCS for model input uncertainties up to 50%. Using FOVA to calculate APLE STP prediction uncertainties using the Maryland data set, the mean measured STP for nearly all site years fell within the 95% confidence intervals of the STP prediction uncertainties. Our results provide users of APLE insight into what model inputs require the most careful measurement when using the model to predict changes in STP under conditions of P drawdown (i.e., no P application) or P buildup., (© 2022 University of Maryland, Colleger Park. Journal of Environmental Quality © 2022 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. This article has been contributed to by US Government employees and their work is in the public domain in the USA.)

Prior to the Affordable Care Act (ACA), insurance companies could charge higher premiums, or outright deny coverage, to people with preexisting health problems. But the ACA's "guaranteed issue" provision forbids such price discrimination and denials of coverage. This paper seeks to determine whether, after implementation of the ACA, nongroup private insurance plans have experienced adverse selection. Our empirical approach employs a copula-based hurdle regression model, with dependence modeled as a function of dimensions along which adverse selection might occur. Our main finding is that, after implementation of the ACA, nongroup insurance enrollees with preexisting health problems do not appear to exhibit adverse selection. This finding suggests that the ACA's mandate that everyone acquire coverage might have attracted enough healthy enrollees to offset any adverse selection., (© 2021 John Wiley & Sons Ltd.)

Identification with all humanity measured as an individual characteristic is an important factor related to social and international relations, such as concern for global issues and human rights, prosocial attitudes, intergroup forgiveness, attitudes toward immigrants, solving global problems, reactions to hate crimes and dehumanisation. We examine the factorial structure, psychometric properties and measurement invariance of the Identification with All Humanity (IWAH) scale in student samples from five countries (the United States, Poland, France, Mexico and Chile; N = 1930). Separate confirmatory factor analyses (CFAs) for each country showed a second-order model of one superordinate factor with two subfactors. The cross-country validation of the scale, based on multigroup CFA, confirmed configural and metric invariance between countries for raw scores, and full metric invariance for "pure" scores. This study showed that the IWAH scale can be successfully used for cross-country research and the results from different countries can be compared and integrated., (© 2020 International Union of Psychological Science.)

The success of poultry litter reuse in U.S. poultry production can be attributed to the efficient treatment methods used by producers during downtimes (the time lapse between consecutive flocks, during which the broiler house is empty). During this period, reused litter may be decaked, tilled/windrowed, or treated with acid-based amendments to reduce ammonia and bacteria levels. Competitive exclusion, pH, and temperature are proposed factors that influence the level of pathogens and the overall litter microbiome during downtimes. We previously reported on the bacterial genetic factors associated with the fitness of two strains of Salmonella enterica serovar Heidelberg (SH) incubated for 14 d in reused litter. Here, we investigated the physicochemical parameters and the microbiome of the litter correlating with SH abundance during this period. We used 16S ribosomal RNA gene sequencing to determine the litter microbiome and whole genome sequencing to characterize bacteria with competitive exclusion potential against SH. The β diversity of the litter microbiome was significantly affected by the duration of incubation, microcosm, and microcosm plus Heidelberg strain combinations. In addition, β diversity was significantly affected by litter parameters, including NH 4 , pH, moisture, water activity, and aluminum. The major phyla observed in the reused litter throughout the 14-d incubation experiment were Firmicutes and Actinobacteria, although their abundance differed by microcosm and time. Amplicon-specific variants homologous to the members of the genera Nocardiopsis and Lentibacillus and the family Bacillaceae_2 were found to significantly correlate with the abundance of Salmonella. A consortium of Bacillus subtilis strains isolated from the litter microcosms reduced the growth of SH in vitro., (© 2020 The Authors. Journal of Environmental Quality published by Wiley Periodicals LLC on behalf of American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. This article has been contributed to by US Government employees and their work is in the public domain in the USA.)

It has been argued that the high achievement of Confucian Asian students is at the cost of their psychological well-being, since high self-doubt consistently accompanies their high achievement. However, other researchers cautioned that the attitude toward self-doubt could be different in Asian versus Western cultures. This study examined the debate with a survey of both American and Chinese college students that measured level of self-doubt, attitude toward self-doubt, beliefs about ability, and psychological well-being outcomes. As hypothesized, Chinese students showed a more positive attitude toward self-doubt than American students, despite having higher level of self-doubt. Furthermore, self-doubt engendered less negative consequences on Chinese students' psychological well-being, relative to American students. Implications for theories and research on cultural differences in the effects of self-constructs are discussed., (© 2018 International Union of Psychological Science.)

The objective of this study was to determine if buffering mepivacaine HCL (mepHCl) with sodium bicarbonate (NaHCO 3 ) would significantly decrease the time to onset of analgesia when performing median and ulnar nerve blocks in naturally lame horses. Median and ulnar nerve blocks were performed on the naturally lame limb of nine horses during two separate study periods, with a minimum washout period of three days between study periods. Nerve blocks were performed by administering mepHCl alone or mepHCl mixed with NaHCO 3 (nine parts 2 per cent mepHCl to one part 8.4 per cent NaHCO 3 ). Lameness was evaluated objectively using a wireless, inertial, sensor-based, motion analysis system (Lameness Locator) prior to the high regional nerve block and every five minutes following administration of the nerve block for 75 min. Resolution of lameness occurred earlier and was more profound for horses administered median and ulnar nerve blocks performed with mepHCl and NaHCO 3 than when these nerve blocks were performed using only mepHCl., Competing Interests: Competing interests: None declared., (© British Veterinary Association 2019. No commercial re-use. See rights and permissions. Published by BMJ.)

This paper presents a copula-based method for identifying and estimating the coefficient of a binary endogenous regressor in a Poisson regression. The method offers advantages over existing approaches. Most importantly, it relies upon standard maximum likelihood approaches, and it does not require numerical integration. Further, as part of its implementation, the method provides a convenient test for the presence of endogeneity. The empirical application investigates the effect of insurance status (a binary measure) on doctor visits (a count measure)., (Copyright © 2017 John Wiley & Sons, Ltd.)

Objective: To describe and validate a technique for sonographically guided posterior cruciate ligament (PCL) injections., Design: Prospective, cadaveric laboratory investigation., Setting: Procedural skills laboratory., Subjects: Eight unembalmed, cadaveric, mid-thigh-knee specimens (4 left knees and 4 right knees) obtained from 4 male and 4 female donors aged 57 to 64 years (mean 60.8 years) with body mass indices of 27.7 to 36.5 kg/m(2) (mean 32 kg/m(2))., Methods: A 5-2-MHz curvilinear probe and a 22-gauge, 78-mm stainless steel needle was used to inject 2 mL of diluted blue latex into the PCL of each specimen using an in-plane, caudad-to-cephalad approach. At a minimum of 24 hours postinjection, each specimen was dissected to assess the presence and distribution of latex within the PCL., Main Outcome: Presence and distribution of latex within the PCL., Results: All 8 injections accurately delivered latex throughout the PCL, including the tibial and femoral footprints. In 2 of 8 specimens (25%), a small amount of latex was noted to extend beyond the PCL and into the joint space. No specimens exhibited evidence of needle injury of latex infiltration with respect to the popliteal neurovascular bundle, menisci, hyaline cartilage, or anterior cruciate ligament., Conclusions: Sonographically guided intraligamentous PCL injections are technically feasible and can be performed with a high degree of accuracy. Sonographically guided PCL injections should be considered for research and clinical purposes to deliver therapeutic agents into the PCL postinjury or postreconstruction., (Copyright © 2016 American Academy of Physical Medicine and Rehabilitation. Published by Elsevier Inc. All rights reserved.)

Objective: To describe and validate a practical technique for sonographically guided anterior cruciate ligament (ACL) injections., Design: Prospective, cadaveric laboratory investigation., Setting: Procedural skills laboratory in a tertiary medical center., Subjects: Ten unembalmed, cadaveric mid-thigh-knee-ankle foot specimens (5 left knees and 5 right knees; 5 male and 5 female) from 10 donors aged 76 to 93 years (mean 85.6 years) with body mass indices of 17.6 to 42.2 kg/m(2) (mean 28.8 kg/m(2))., Methods: A single, experienced operator used a 22-gauge, 63.5-mm stainless steel needle and a 12-3-MHz linear transducer to inject 1.5 mL of diluted colored latex into the ACLs of 10 unembalmed cadaveric specimens via an in-plane, caudad-to-cephalad approach, long axis to the ACL. At a minimum of 24 hours postinjection, specimens were dissected, and the presence and distribution of latex within the ACL assessed by a study co-investigator., Main Outcome: Presence and distribution of latex within the ACL., Results: All 10 injections accurately delivered latex into the proximal (femoral), midsubstance, and distal (tibial) portions of the ACL. No specimens exhibited evidence of needle injury or latex infiltration with respect to the menisci, hyaline cartilage, or posterior cruciate ligament., Conclusions: Sonographically guided intra-ligamentous ACL injections are technically feasible and can be performed with a high degree of accuracy. Sonographically guided ACL injections could be considered for research and clinical purposes to directly deliver injectable agents into the healing ACL postinjury or postreconstruction., (Copyright © 2015 American Academy of Physical Medicine and Rehabilitation. Published by Elsevier Inc. All rights reserved.)

In most states, the phosphorus (P) index (PI) is the adopted strategy for assessing a field's vulnerability to P loss; however, many state PIs have not been rigorously evaluated against measured P loss data to determine how well the PI assigns P loss risk-a major reason being the lack of field data available for such an analysis. Given the lack of P loss data available for PI evaluation, our goal was to demonstrate how a P loss model can be used to evaluate and revise a PI using the Pennsylvania (PA) PI as an example. Our first objective was to compare two different formulations-multiplicative and component-for calculating a PI. Our second objective was to evaluate whether output from a P loss model can be used to improve PI weighting by calculating weights for modified versions of the PA PI from model-generated P loss data. Our results indicate that several potential limitations exist with the original multiplicative index formulation and that a component formulation is more consistent with how P loss is calculated with P loss models and generally provides more accurate estimates of P loss. Moreover, using the PI weights calculated from the model-generated data noticeably improved the correlation between PI values and a large and diverse measured P loss data set. The approach we use here can be used with any P loss model and PI and thus can serve as a guide to assist states in evaluating and modifying their PI., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

The incorporation of biochar into soils has been proposed as a means to sequester carbon from the atmosphere. An added environmental benefit is that biochar has also been shown to increase soil retention of nutrients, heavy metals, and pesticides. The goal of this study was to evaluate whether biochar amendments affect the transport of Escherichia coli through a water-saturated soil. We looked at the transport of three E. coli isolates through 10-cm columns packed with a fine sandy soil amended with 2 or 10% (w/w) poultry litter biochar pyrolyzed at 350 or 700°C. For all three isolates, mixing the high-temperature biochar at a rate of 2% into the soil had no impact on transport behavior. When added at a rate of 10%, a reduction of five orders of magnitude in the amount of E. coli transported through the soil was observed for two of the isolates, and a 60% reduction was observed for the third isolate. Mixing the low-temperature biochar into the soil resulted in enhanced transport through the soil for two of the isolates, whereas no significant differences in transport behavior were observed between the low-temperature and high-temperature biochar amendments for one isolate. Our results show that the addition of biochar can affect the retention and transport behavior of E. coli and that biochar application rate, biochar pyrolysis temperature, and bacterial surface characteristics were important factors determining the transport of E. coli through our test soil., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

Alternative N fertilizers that produce low greenhouse gas (GHG) emissions from soil are needed to reduce the impacts of agricultural practices on global warming potential (GWP). We quantified and compared growing season fluxes of NO, CH, and CO resulting from applications of different N fertilizer sources, urea (U), urea-ammonium nitrate (UAN), ammonium nitrate (NHNO), poultry litter, and commercially available, enhanced-efficiency N fertilizers as follows: polymer-coated urea (ESN), SuperU, UAN + AgrotainPlus, and poultry litter + AgrotainPlus in a no-till corn ( L.) production system. Greenhouse gas fluxes were measured during two growing seasons using static, vented chambers. The ESN delayed the NO flux peak by 3 to 4 wk compared with other N sources. No significant differences were observed in NO emissions among the enhanced-efficiency and traditional inorganic N sources, except for ESN in 2009. Cumulative growing season NO emission from poultry litter was significantly greater than from inorganic N sources. The NO loss (2-yr average) as a percentage of N applied ranged from 0.69% for SuperU to 4.5% for poultry litter. The CH-C and CO-C emissions were impacted by environmental factors, such as temperature and moisture, more than the N source. There was no significant difference in corn yield among all N sources in both years. Site specifics and climate conditions may be responsible for the differences among the results of this study and some of the previously published studies. Our results demonstrate that N fertilizer source and climate conditions need consideration when selecting N sources to reduce GHG emissions., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

Ammonia volatilization from the mineralization of uric acid and urea has a major impact on the poultry industry and the environment. Dry acids are commonly used to reduce ammonia emissions from poultry houses; however, little is known about how acidification affects the litter biologically. The goal of this laboratory incubation was to compare the microbiological and physiochemical effects of dry acid amendments (Al+Clear, Poultry Litter Treatment, Poultry Guard) on poultry litter to an untreated control litter and to specifically correlate uric acid and urea contents of these litters to the microbes responsible for their mineralization. Although all three acidifiers eventually produced similar effects within the litter, there was at least a 2-wk delay in the microbiological responses using Poultry Litter Treatment. Acidification of the poultry litter resulted in >3 log increases in total fungal concentrations, with both uricolytic (uric acid degrading) and ureolytic (urea degrading) fungi increasing by >2 logs within the first 2 to 4 wk of the incubation. Conversely, total, uricolytic, and ureolytic bacterial populations all significantly declined during this same time period. While uric acid and urea mineralization occurred within the first 2 wk in the untreated control litter, acidification resulted in delayed mineralization events for both uric acid and urea (2 and 4 wk delay, respectively) once fungal cell concentrations exceeded a threshold level. Therefore, fungi, and especially uricolytic fungi, appear to have a vital role in the mineralization of organic N in low-pH, high-N environments, and the activity of these fungi should be considered in best management practices to reduce ammonia volatilization from acidified poultry litter.

Wastewater quality and malodors in a second generation implementation of environmentally superior technology (EST) were monitored over three cycles of pig (Sus scrofa) production and 15 mo. The wastewater treatment system consisted of three modules: solids separation, biological N removal, and P recovery/wastewater disinfection. While approximately more than 90% of the wastewater suspended solids were removed in the first stage of treatment, little reduction in malodorous compounds occurred, indicating that malodors largely remained with the liquid waste stream. The greatest improvements in wastewater quality occurred in the N treatment module where ammonium was removed through nitrification/denitrification processes: there was more than 99% reduction in aromatic malodorous compounds (e.g., p-cresol, skatole) and almost 90% reduction in volatile fatty acids (e.g., propanoate and butanoate) in N module effluent as compared to raw flushed manure. The system performed consistently well in wastewater odor removal, even during the first cycle of livestock production when system performance was being optimized. These findings showed that the combination of the processes of solids removal and biological N treatment into a practical treatment system can be very effective in reducing malodors from livestock wastewater.

The inability to incorporate manure into permanent pasture leads to the concentration of nutrients near the soil surface with the potential to be transported off site by runoff water. In this study, we used rainfall simulations to examine the effect of broiler chicken (Gallus gallus domesticus) litter application method and the runoff timing on nutrient and E. coli losses from tall fescue (Festuca arundinacea Schreb.) pasture on a Hartsells sandy loam soil (fine-loamy, siliceous, subactive, thermic Typic Hapludults)) in Crossville, AL. Treatments included two methods of litter application (surface broadcast and subsurface banding), commercial fertilizer, and control. Litter was applied at a rate of 8.97 Mg ha(-1). Treatments were assigned to 48 plots with four blocks (12 plots each) arranged in a randomized complete block design to include three replications in each block. Simulated rainfall was applied to treatments as follows: Day 1, block 1 (runoff 1); Day 8, block 2 (runoff 2); Day 15, block 3 (runoff 3); and Day 22, block 4 (runoff 4). Total phosphorus (TP), inorganic N, and Escherichia coli concentrations in runoff from broadcast litter application were all significantly greater than from subsurface litter banding. The TP losses from broadcast litter applications averaged 6.8 times greater than those from subsurface litter applications. About 81% of the runoff TP was in the form of dissolved reactive phosphorus (DRP) for both litter-application methods. The average losses of NO(3)-N and total suspended solids (TSS) from subsurface banding plots were 160 g ha(-1) and 22 kg ha(-1) compared to 445 g ha(-1) and 69 kg ha(-1) for the broadcast method, respectively. Increasing the time between litter application and the first runoff event helped decrease nutrient and E. coli losses from surface broadcast litter, but those losses generally remained significantly greater than controls and subsurface banded, regardless of runoff timing. This study shows that subsurface litter banding into perennial grassland can substantially reduce nutrient and pathogen losses in runoff compared to the traditional surface-broadcast practice.

Escherichia coli is a commonly used indicator organism for detecting the presence of fecal-borne pathogenic microorganisms in water supplies. The importance of E. coli as an indicator organism has led to numerous studies looking at cell properties and transport behavior of this microorganism. In many of these studies, however, only a single strain of E. coli was used even though research has shown that significant genetic variability exists among different strains of E. coli. If this genetic diversity results in differences in cell properties that affect transport, different strains of E. coli may exhibit different rates of transport in the environment. Therefore, the objectives of our study were to investigate the variability in surface characteristics and transport behavior of E. coli isolates obtained from six different sources: beef cattle, dairy cattle, horse, human, poultry, and wildlife. Cell properties such as electrophoretic mobility, cell size and shape, hydrophobicity, charge density, and extracellular polymeric substance composition were measured for each isolate. In addition, the transport behavior of each isolate was assessed by measuring transport through 10-cm packed beds of clean quartz sand. Our results show a large diversity in cell properties and transport behavior for the different E. coli isolates. This diversity in transport behavior must be taken into account when making assessments of the suitability of using E. coli as an indicator organism for specific pathogenic microorganisms in groundwater environments as well as modeling the movement of E. coli in the subsurface.

Microbial mineralization of urea and uric acid in poultry litter results in the production of ammonia, which can lead to decreased poultry performance, malodorous emissions, and loss of poultry litter value as a fertilizer. Despite the fact that this is a microbial process, little is known about how the microbial populations, especially ammonia-producing (ureolytic) organisms in poultry litter, respond to litter amendments such as aluminum sulfate (Al(2)(SO(4))(3).14H(2)O; alum). The goal of this study was to measure the temporal changes in total bacterial and fungal populations and urease-producing microorganisms in nontreated litter or litter treated with 10% alum. Quantitative real-time polymerase chain reaction was used to target the bacterial 16S rRNA gene, the fungal 18S rRNA gene, or the urease gene of bacterial and fungal ammonia producers in a poultry litter incubation study. Nontreated poultry litter had relatively high total (2.8 +/- 0.8 x 10(10) cells g(-1) litter) and ureolytic (2.8 +/- 1.3 x 10(8) cells g(-1) litter) bacterial populations. Alum treatment reduced the total bacterial population by 50% and bacterial urease producers by 90% within 4 wk. In contrast, at 16 wk after alum treatment, the fungal population was three orders of magnitude higher in alum-treated litter than in nontreated litter (3.5 +/- 0.8 x 10(7) cells g(-1) litter and 5.5 +/- 2.5 x 10(4) cells g(-1) litter, respectively). The decrease in pH produced by alum treatment is believed to inhibit bacterial populations and favor growth of fungi that may be responsible for the mineralization of organic nitrogen in alum-treated litters.

The Langmuir model is commonly used for describing the sorption behavior of reactive solutes to surfaces and is often fit to sorption data using nonlinear least squares regression. An important assumption of least squares regression is that the predictor variable is error free. In the case of sorption data, this assumption is not valid, and therefore the potential for parameter bias exists. Although alternative regression methods exist that either explicitly account for error in the predictor variable (Model II regression) or minimize the error in the predictor variable, these methods are not commonly used. Therefore, this paper more fully explores the differences in fitted parameters and model fits between these different data fitting methods by fitting P sorption data collected on 26 different soil samples using three different regression methods. For a majority of soils tested in this study, the differences in model fits between the three regression methods were not statistically significant. Statistical differences were observed in over a third of the soils, however, suggesting that errors in the predictor variable may be large enough to produce biased parameter estimates. These results suggest that multiple regression methods should be used when fitting the Langmuir model to sorption data to better assess the potential impact of error on model fits.

Pasture management and broiler litter application rate are critical factors influencing the magnitude of nutrients being transported by runoff from fields. We investigated the impact of pasture management and broiler litter application rate on nutrient runoff from bermudagrass (Cynodon dactylon) pastures. The experiment was conducted on a Ruston fine sandy loam with a factorial arrangement on 21 large paddocks. Runoff water was collected from natural rainfall events from 2001 to 2003. Runoff water and soil samples were analyzed for nutrients and sediments. Runoff was generally greater (29%) from grazed than hayed pastures regardless of the litter application rate. There was greater inorganic N in the runoff from grazed paddocks when litter rate was based on N rather than P. The mean total P loss per runoff event for all treatments ranged from 7 to 45 g ha(-1) and the grazed treatment with litter applied on N basis had the greatest total P loss. Total dissolved P was the dominant P fraction in the runoff, ranging from 85% to 93% of the total P. The soluble reactive P was greater for treatments with litter applied on N basis regardless of pasture management. Runoff total sediments were greater for N-based litter application compared to those which received litter on P basis. Our results indicate that litter may be applied on N basis if the pasture is hayed and the soil P is low. In contrast, litter rates should be based on a P-basis if pasture is grazed.

Poultry litter treatment with alum (Al(2)(SO(4))(3) . 18H(2)O) lowers litter phosphorus (P) solubility and therefore can lower litter P release to runoff after land application. Lower P solubility in litter is generally attributed to aluminum-phosphate complex formation. However, recent studies suggest that alum additions to poultry litter may influence organic P mineralization. Therefore, alum-treated and untreated litters were incubated for 93 d to assess organic P transformations during simulated storage. A 62-d soil incubation was also conducted to determine the fate of incorporated litter organic P, which included alum-treated litter, untreated litter, KH(2)PO(4) applied at 60 mg P kg(-1) of soil, and an unamended control. Liquid-state (31)P nuclear magnetic resonance indicated that phytic acid was the only organic P compound present, accounting for 50 and 45% of the total P in untreated and alum-treated litters, respectively, before incubation and declined to 9 and 37% after 93 d of storage-simulating incubation. Sequential fractionation of litters showed that alum addition to litter transformed 30% of the organic P from the 1.0 mol L(-1) HCl to the 0.1 mol L(-1) NaOH extractable fraction and that both organic P fractions were more persistent in alum-treated litter compared with untreated litter. The soil incubation revealed that 0.1 mol L(-1) NaOH-extractable organic P was more recalcitrant after mixing than was the 1.0 mol L(-1) HCl-extractable organic P. Thus, adding alum to litter inhibits organic P mineralization during storage and promotes the formation of alkaline extractable organic P that sustains lower P solubility in the soil environment.

The concentrations of malodorous compounds in a 0.4-ha anaerobic lagoon that received waste from approximately 2000 sows were monitored during the late summer to late fall of 2006 to gain insight into the factors influencing their concentrations. Selected malodorous compounds were measured by the use of equilibrium samplers consisting of submersible stir plates and stir bar sorbtive sampling with polydimethylsiloxane-coated magnetic stir bars. During the same period, air and water temperatures, suspended solids, total organic carbon and nitrogen content, and wastewater pH were recorded. Concentrations of malodorous compounds were higher at the surface of the lagoon than at the middle or bottom of the lagoon. Skatole concentration, for instance, averaged 54, 24, and 38 microg L(-1) near the surface, in the middle, and at the lowest sampling depths, respectively. While the lagoon was being pumped down during field application of wastewater, concentrations of malodorous compounds fluctuated widely, increased 16-fold as compared with the sampling period before pumping, and continued to increase as fall progressed and temperatures cooled. Suspended solids, volatile suspended solids, and total organic carbon increased near the bottom of the lagoon during this same period. The increases in the concentrations of malodorous compounds in the wastewater during the fall could have been due to a combination of several factors. These factors include reduced degradation by lagoon bacteria, less wind stripping of volatile compounds from the lagoon surface due to lowering of the lagoon surface after crop application, and/or reduced evaporation of malodorous compounds due to falling temperatures.

Background: Pedestrian injury is the third leading cause of unintentional injury and death among children 1-14 years old. The purpose of this study was to examine primary grade teachers' perceived role in pedestrian safety education., Methods: A total of 630 surveys were sent to a national random sample of primary school teachers and 54% responded., Results: The results of the study found that 85% of primary grade teachers believed it was extremely important or important to teach pedestrian safety. Forty percent of the teachers identified that they had been teaching pedestrian safety for more than 1 year (maintenance stage), whereas 1 in 3 teachers had never thought about (precontemplation stage) teaching pedestrian safety to their students. Eighty-eight percent of primary grade teachers were very confident about teaching students how to safely cross the street, where to safely cross the street (87%), when to safely cross the street (86%), and how to safely walk along streets with sidewalks (83%). An examination of how pedestrian safety information was presented found that a plurality (39%) of the teachers who taught pedestrian safety presented information through classroom discussion. Also, 46% of the teachers who taught pedestrian safety identified the following as the most common curriculum content: when to safely cross the street (46%), how to safely cross the street (45%), and where to safely cross the street (43%)., Conclusions: Only 16% of primary school teachers reported pedestrian safety education was required for their grade levels. Yet, pedestrian safety education decreases pedestrian-related deaths. Therefore, it is important for schools and primary grade teachers to expose their students to this essential topic.

Due to the difficulties in testing for specific pathogens, water samples are tested for the presence of nonpathogenic indicator organisms to determine whether a water supply has been contaminated by fecal material. An implicit assumption in this approach is that where pathogenic microorganisms are present fecal indicator organisms are present as well; yet surprisingly few studies have been conducted that directly compare the transport of indicator organisms with pathogenic organisms in ground water environments. In this study we compared the cell properties and transport of Escherichia coli, a commonly used indicator organism, and Campylobacter jejuni, an important enteropathogen commonly found in agricultural wastes, through saturated porous media. Differences in cell properties were determined by measuring cell geometry, hydrophobicity, and electrophoretic mobility. Transport differences were determined by conducting miscible displacement experiments in laboratory columns. Under the experimental conditions tested, C. jejuni was much more negatively charged and more hydrophobic than E. coli. In addition, C. jejuni cells were slightly longer, narrower, and less spherical than E. coli. The variations in cell properties, primarily surface charge, resulted in significant differences in transport between these two microorganisms, with the transport of C. jejuni exceeding that of E. coli when conditions favored low attachment rates, thus calling into question the usefulness of using E. coli as an indicator organism for this important pathogen.

There is a need for treatment technologies that can eliminate environmental problems associated with anaerobic lagoons. These technologies must be able to capture nutrients, kill pathogens, and reduce emissions of ammonia and nuisance odors. To meet these needs, a full-scale wastewater treatment plant was installed as a demonstration project on one of three 4360-pig (Sus scrofa) production units in a finishing farm in Duplin County, North Carolina. Once the treatment plant was operational, flow of raw manure into the unit's corresponding lagoon was discontinued and the lagoon was used to store treated wastewater. Water quality was monitored in the converted lagoon and in the two conventional lagoons. A gas chromatographic method was developed to measure concentration of five selected malodorous compounds (phenol, p-cresol, 4-ethylphenol, indole, and skatole) in liquid lagoon samples. Dramatic improvements in the water quality parameters TKN, NH3-N, solids, COD, and BOD in the converted waste lagoon paralleled reductions in malodorous compounds. Nine months after conversion, identified malodorous compounds in liquid extractions averaged 6.6 and 38.8 ng mL(-1) in water from the converted lagoon and the conventional lagoons, respectively. The reduction was particularly marked for p-cresol, 4-ethylphenol, and skatole, all of which make important contributions to swine waste odors due to their characteristic odors and low detection thresholds.

In 1997 a statewide random digit-dialed telephone survey of 534 South Carolina registered voters was conducted to determine level of support for school-based reproductive health services. Results indicated most voters supported services in secondary schools that would provide students with information and counseling about contraceptive methods and refer students to community-based agencies for abstinence education and counseling. Voters were less supportive of reproductive health services that would diagnose or treat sexually transmitted diseases, test for pregnancy, refer students to community-based agencies for contraceptives, or make contraceptives available to students at school. Significant associations in support for school-based reproductive health services were reported based on income, personal beliefs, intentions, and outcome expectations regarding sexuality education and contraceptive availability. These data reinforce the need for school-aged youth in South Carolina to have access to reproductive health services within their communities, outside of the school setting.

Research has demonstrated that recognition of sexual orientation begins in adolescence. However, students who identify as gay or lesbian report that the subject of homosexuality is virtually absent from classroom instruction. In South Carolina public schools, the subject of homosexuality may not be discussed except during instruction about sexually transmitted diseases. In 1997, survey data were obtained from 534 South Carolina registered voters to determine level of support for school-based sexuality education, including support for instruction about homosexuality. Overall, support for sexuality education was strong, however, homosexuality was the least-supported subject in the survey. In addition, registered voters were less sure as to what grade level instruction about homosexuality should begin. Characteristics of voters who supported and opposed instruction about homosexuality in the public schools were examined and compared. These data may be useful in building support for sexuality education programs that address this controversial topic.