1. Paving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing
- Author
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Valverde de la Fuente, Alejandro, Gordón Pidal, José María, Montero Calle, Ana, Arévalo Pérez, Beatriz, Serafín González-Carrato, Verónica, Calero, Miguel, Moreno Guzmán, María, López, Miguel Ángel, Escarpa, Alberto, Yáñez Sedeño, Paloma, Barderas, Rodrigo, Campuzano Ruiz, Susana, Pingarrón Carrazón, José Manuel, Valverde de la Fuente, Alejandro, Gordón Pidal, José María, Montero Calle, Ana, Arévalo Pérez, Beatriz, Serafín González-Carrato, Verónica, Calero, Miguel, Moreno Guzmán, María, López, Miguel Ángel, Escarpa, Alberto, Yáñez Sedeño, Paloma, Barderas, Rodrigo, Campuzano Ruiz, Susana, and Pingarrón Carrazón, José Manuel
- Abstract
CRUE-CSIC (Acuerdos Transformativos 2022), Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values., Ministerio de Ciencia e Innovación (MICINN), Instituto de Salud Carlos III (ISCIII), Comunidad de Madrid, I, Depto. de Química Analítica, Depto. de Química en Ciencias Farmacéuticas, Fac. de Ciencias Químicas, Fac. de Farmacia, TRUE, pub
- Published
- 2022