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Your search keyword '"Han, Shengli"' showing total 19 results
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19 results on '"Han, Shengli"'

3. Pseudo‐allergic compounds screened from Shengmai injection by using high‐expression Mas‐related G protein‐coupled receptor X2 cell membrane chromatography online coupled with liquid chromatography and mass spectrometry

Author

Sun, Wei, primary, Wang, Saisai, additional, Liang, Peida, additional, Zhou, Huaxin, additional, Zhang, Liyang, additional, Jia, Qianqian, additional, Fu, Jia, additional, Lv, Yanni, additional, and Han, Shengli, additional

Published
2021
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9. Typical antimicrobials induce mast cell degranulation and anaphylactoid reactions via MRGPRX2 and its murine homologue MRGPRB2

Author

Zhang, Tao, primary, Che, Delu, additional, Liu, Rui, additional, Han, Shengli, additional, Wang, Nan, additional, Zhan, Yingzhuan, additional, Pundir, Priyanka, additional, Cao, Jiao, additional, Lv, Yanni, additional, Yang, Liu, additional, Wang, Jue, additional, Ding, Meiwen, additional, Dong, Xinzhong, additional, and He, Langchong, additional

Published
2017
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18. Screening potential antagonists of epidermal growth factor receptor from Marsdenia tenacissima via cell membrane chromatography model assisted by HPLC-ESI-IT-TOF-MS.

Author

Lv Y, Shi X, Fu J, Jia Q, Lin Y, Wang H, Yang X, and Han S

Subjects
A549 Cells, Chromatography, High Pressure Liquid methods, Drug Discovery, Humans, MCF-7 Cells, Molecular Docking Simulation, Cell Membrane metabolism, Chromatography, Affinity methods, Drugs, Chinese Herbal analysis, Drugs, Chinese Herbal metabolism, ErbB Receptors antagonists & inhibitors, Marsdenia chemistry
Abstract

Marsdenia tenacissima, or Tongguanteng in Chinese, is a traditional Chinese herb and has a broad application in clinical practice for its pharmacological effects of treating asthma, pneumonia, tonsillitis, pharyngitis tumors, etc. However, few studies have reported the screening of the active components of this medicine for tumor therapy. In this work, a two-dimensional analytical system was developed to screen antagonists of epidermal growth factor receptor (EGFR) from M. tenacissima. A fraction was retained on the EGFR cell membrane chromatography (CMC) column, separated and identified as tenacissoside G (TG), tenacissoside H (TH) and tenacissoside I (TI) by two-dimensional HPLC-IT-TOF-MS. Molecular docking and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay were carried out to assess the activity of TS (including TG, TH and TI). Molecular docking results showed that the binding mode of TS on EGFR is similar to that of gefitinib. The MTT assay demonstrated that gefitinib and TS (especially TI) could inhibit the growth of EGFR highly expressed cell lines in a dose-dependent manner in the range of 5-50 μmol/L. In conclusion, the two-dimensional EGFR/CMC-HPLC-IT-TOF-MS system could be a useful approach in drug discovery from traditional Chinese medicines for searching for potential antitumor candidates., (© 2019 John Wiley & Sons, Ltd.)

Published
2019
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19. Screening anti-allergic components of Astragali Radix using LAD2 cell membrane chromatography coupled online with UHPLC-ESI-MS/MS method.

Author

Lv Y, Sun Y, Fu J, Kong L, and Han S

Subjects
Anti-Allergic Agents analysis, Astragalus propinquus chemistry, Cell Line, Cell Membrane drug effects, Cell Membrane metabolism, Drugs, Chinese Herbal chemistry, Histamine metabolism, Histamine Antagonists analysis, Histamine Antagonists pharmacology, Humans, Isoflavones analysis, Mast Cells metabolism, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods, Anti-Allergic Agents pharmacology, Chromatography, High Pressure Liquid methods, Drug Evaluation, Preclinical methods, Drugs, Chinese Herbal pharmacology, Isoflavones pharmacology, Mast Cells drug effects
Abstract

Huangqi (Astragali Radix), a traditional Chinese herb, is widely used in clinical therapy in China. In addition, an anti-allergic effect of constituents in Huangqi has been reported in the scientific literature. In the present study, cell membrane chromatography coupled online with UHPLC-ESI-MS/MS method was developed to screen, analyze and identify the anti-allergic components of Huangqi. The Laboratory of Allergic Disease 2 (LAD2) cell was used to establish cell membrane chromatography, which was combined with UHPLC-ESI-MS/MS. The coupled system was then used to screen anti-allergic components from Huangqi. Effects of active components were verified by histamine release assay. A component retained on the LAD2 cell membrane chromatography was identified as formononetin. Bioactivity of formononetin was investigated by histamine release assay in LAD2 cells, and it was found that formononetin could inhibit histamine release in a dose-dependent manner from 1 to 100 μm. The LAD2 cell membrane chromatography online with UHPLC-ESI-MS/MS method is an effective technique for screening the anti-allergic components of Huangqi., (Copyright © 2016 John Wiley & Sons, Ltd.)

Published
2017
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