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Your search keyword '"Hattori, Masa-Aki"' showing total 12 results
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12 results on '"Hattori, Masa-Aki"'

5. Expression of rat uterine serine proteinases homologous to mouse implantation serine proteinase 2

Author

Oozono, Shinji, primary, Yamauchi, Nobuhiko, additional, Nishimura, Kyohei, additional, Matsumoto, Kenji, additional, Watanabe, Ryo, additional, Kubota, Kaiyu, additional, Aramaki, Shinya, additional, Sato, Fuminori, additional, Wood, Chris, additional, Soh, Tomoki, additional, Kizaki, Kei‐Ichirou, additional, and Hattori, Masa‐Aki, additional

Published
2008
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6. Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro

Author

Gamo, Toru, primary, Yamauchi, Nobuhiko, additional, Nishimura, Kyohei, additional, Watanabe, Ryo, additional, Matsumoto, Kenji, additional, Oozono, Shinji, additional, Kubota, Kaiyu, additional, He, Pei-Jian, additional, Soh, Tomoki, additional, and Hattori, Masa-aki, additional

Published
2007
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12. Development of an in vitro model for the analysis of bovine endometrium using simple techniques.

Author

Yamauchi K, Yamauchi N, Yamagami K, Nakamura N, Yamashita S, Islam MR, Tabata S, Yahiro K, Tamura T, Hashizume K, and Hattori MA

Subjects
Animals, Cattle, Cells, Cultured, Estradiol pharmacology, Estrogen Receptor alpha genetics, Estrogen Receptor alpha metabolism, Female, Fluorescent Antibody Technique, Hepatocyte Growth Factor genetics, Hepatocyte Growth Factor metabolism, Matrix Metalloproteinases genetics, Matrix Metalloproteinases metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction, Receptors, Interferon genetics, Receptors, Interferon metabolism, Receptors, Oxytocin genetics, Receptors, Oxytocin metabolism, Receptors, Progesterone genetics, Receptors, Progesterone metabolism, Spheroids, Cellular metabolism, Staining and Labeling, Endometrium cytology, Endometrium metabolism, Models, Biological
Abstract

This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero-spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells, except the glandular-like structure of the epithelial cells inside the explants. Gelatin zymography revealed that the hetero-spheroids did not express matrix metalloproteinases (MMPs) after 4 days of culture, but strong MMP expressions were observed in the cultured explants until 7 days of culture. Additionally, expression of progesterone receptor (PR), estrogen receptor alpha (ERα), type I interferon receptor 1 (IFNAR1) and 2 (IFNAR2) messenger RNA was observed both in the homo- and hetero-spheroids. The expression of oxytocin receptor (OTR) mRNA in E2 and E2+P4 (1,3,5(10)-Estratrien-3, 17β-diol + 4-Pregnen-3, 20-dinone) treated groups were significantly (P < 0.05) higher than that of the control group of spheroids. In case of cultured explants, the expression of PR and OTR mRNA were significantly (P < 0.05) higher in E2 treated groups compared to the control groups. Hepatocyte growth factor (HGF) mRNA expression was also higher in P4 treated groups at 10 days in culture (P < 0.05). In a nutshell, the in vitro model developed in this study for the analysis of the endometrium may provide a new platform for extensive research on bovine endometrial function., (© 2014 Japanese Society of Animal Science.)

Published
2015
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