1. Genomic Exploration of the Hemiascomycetous Yeasts: 2. Data generation and processing
- Author
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Simone Duprat, William Saurin, Claude Scarpelli, Patrick Wincker, François Artiguenave, Jean Weissenbach, Phillippe Brottier, Virginie Vico, Jean Verdier, and Fabien Jovelin
- Subjects
Genetics ,High-throughput sequencing ,Genome ,Sequence analysis ,Test data generation ,Sequencing data ,Biophysics ,Computational Biology ,Sequence Analysis, DNA ,Cell Biology ,Computational biology ,Biology ,Biochemistry ,Pichia ,DNA sequencing ,DNA sequencer ,Ascomycota ,Structural Biology ,Hemiascomycete ,Genome, Fungal ,Molecular Biology ,Reaction tube - Abstract
The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.
- Published
- 2000
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