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Your search keyword '"Ishida, Hidekazu"' showing total 14 results
Start Over Author "Ishida, Hidekazu" Publisher wiley
14 results on '"Ishida, Hidekazu"'

1. Gene correction and overexpression of TNNI3 improve impaired relaxation in engineered heart tissue model of pediatric restrictive cardiomyopathy

Author

Hasegawa, Moyu, primary, Miki, Kenji, additional, Kawamura, Takuji, additional, Takei Sasozaki, Ikue, additional, Higashiyama, Yuki, additional, Tsuchida, Masaru, additional, Kashino, Kunio, additional, Taira, Masaki, additional, Ito, Emiko, additional, Takeda, Maki, additional, Ishida, Hidekazu, additional, Higo, Shuichiro, additional, Sakata, Yasushi, additional, and Miyagawa, Shigeru, additional

Published
2024
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2. Riociguat in children with pulmonary arterial hypertension: The PATENT–CHILD study

Author

García Aguilar, Humberto, primary, Gorenflo, Matthias, additional, Ivy, D. Dunbar, additional, Moledina, Shahin, additional, Castaldi, Biagio, additional, Ishida, Hidekazu, additional, Cześniewicz, Paweł, additional, Kusa, Jacek, additional, Miera, Oliver, additional, Pattathu, Joseph, additional, Weng, Ken‐Pen, additional, Ablonczy, Laszlo, additional, Apitz, Christian, additional, Katona, Marta, additional, Kurosaki, Kenichi, additional, Pulido, Tomas, additional, Yamagishi, Hiroyuki, additional, Yasuda, Kazushi, additional, Cisternas, Galia, additional, Goth, Melanie, additional, Lippert, Susanne, additional, Radomskyj, Anna, additional, Saleh, Soundos, additional, Willmann, Stefan, additional, Wirsching, Gabriela, additional, Bonnet, Damien, additional, and Beghetti, Maurice, additional

Published
2022
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8. A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase

Author

Brouilette, Scott, primary, Kuersten, Scott, additional, Mein, Charles, additional, Bozek, Monika, additional, Terry, Anna, additional, Dias, Kerith-Rae, additional, Bhaw-Rosun, Leena, additional, Shintani, Yasunori, additional, Coppen, Steven, additional, Ikebe, Chiho, additional, Sawhney, Vinit, additional, Campbell, Niall, additional, Kaneko, Masahiro, additional, Tano, Nobuko, additional, Ishida, Hidekazu, additional, Suzuki, Ken, additional, and Yashiro, Kenta, additional

Published
2012
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14. Cardiomyocyte differentiation from mouse embryonic stem cells using a simple and defined protocol.

Author

Kokkinopoulos I, Ishida H, Saba R, Coppen S, Suzuki K, and Yashiro K

Subjects
Animals, Mice, Cell Culture Techniques methods, Cell Differentiation, Mouse Embryonic Stem Cells cytology, Myocytes, Cardiac cytology
Abstract

Background: Embryonic stem (ES) cells are pluripotent cells with the ability to differentiate to any cell type of the resident organism. In recent years, significant advances have been made in using these cells to obtain large numbers of cardiomyocyte (CM)-like cells for scientific research and clinical application. A vast number of protocols have emerged describing differentiation methods without the use of animal serum or extracts restrictive for use in a human clinical setting. These techniques follow a complicated procedure, which although successful, show a relatively varied yield among cell batches., Results: We have designed a three-step differentiation protocol using defined reagents and a monolayer culture without feeder cells, avoiding embryoid body formation and multiple trypsin treatment, in which beating foci appeared as early as day 6 in in vitro differentiating conditions. Our results show a high yield of CM reaching approximately 60% of the differentiated cells after 13 days in vitro., Conclusions: We provide a fast, simple, reliable and reproducible protocol for inducing murine ES cells toward a CM-like phenotype comparable to available high-yield protocols, without the use of intermediate trypsinization/passage steps., (© 2015 Wiley Periodicals, Inc.)

Published
2016
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