35 results on '"Levings, Megan K."'
Search Results
2. Targeting regulatory T cell metabolism in disease: novel therapeutic opportunities
- Author
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Halvorson, Torin, primary, Tuomela, Karoliina, additional, and Levings, Megan K., additional
- Published
- 2023
- Full Text
- View/download PDF
3. T cell activation induced marker assays in health and disease
- Author
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Poloni, Chad, primary, Schonhofer, Cole, additional, Ivison, Sabine, additional, Levings, Megan K, additional, Steiner, Theodore S, additional, and Cook, Laura, additional
- Published
- 2023
- Full Text
- View/download PDF
4. PTEN is required for human Treg suppression of costimulation in vitro
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Lam, Avery J., primary, Haque, Manjurul, additional, Ward‐Hartstonge, Kirsten A., additional, Uday, Prakruti, additional, Wardell, Christine M., additional, Gillies, Jana K., additional, Speck, Madeleine, additional, Mojibian, Majid, additional, Klein Geltink, Ramon I., additional, and Levings, Megan K., additional
- Published
- 2022
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5. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
- Author
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Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Abrignani, Sergio, additional, Addo, Richard, additional, Akdis, Mübeccel, additional, Andrä, Immanuel, additional, Andreata, Francesco, additional, Annunziato, Francesco, additional, Arranz, Eduardo, additional, Bacher, Petra, additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Baumjohann, Dirk, additional, Beccaria, Cristian G., additional, Bernardo, David, additional, Boardman, Dominic A., additional, Borger, Jessica, additional, Böttcher, Chotima, additional, Brockmann, Leonie, additional, Burns, Marie, additional, Busch, Dirk H., additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cassotta, Antonino, additional, Chang, Yinshui, additional, Chirdo, Fernando Gabriel, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Cook, Laura, additional, Corbett, Alexandra J., additional, Cornelis, Rebecca, additional, Cosmi, Lorenzo, additional, Davey, Martin S., additional, De Biasi, Sara, additional, De Simone, Gabriele, additional, del Zotto, Genny, additional, Delacher, Michael, additional, Di Rosa, Francesca, additional, Di Santo, James, additional, Diefenbach, Andreas, additional, Dong, Jun, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dutertre, Charles‐Antoine, additional, Eckle, Sidonia B. G., additional, Eede, Pascale, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Feuerer, Markus, additional, Fillatreau, Simon, additional, Fiz‐Lopez, Aida, additional, Follo, Marie, additional, Foulds, Gemma A., additional, Fröbel, Julia, additional, Gagliani, Nicola, additional, Galletti, Giovanni, additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Garrote, José Antonio, additional, Geginat, Jens, additional, Gherardin, Nicholas A., additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Godfrey, Dale I., additional, Gruarin, Paola, additional, Haftmann, Claudia, additional, Hansmann, Leo, additional, Harpur, Christopher M., additional, Hayday, Adrian C., additional, Heine, Guido, additional, Hernández, Daniela Carolina, additional, Herrmann, Martin, additional, Hoelsken, Oliver, additional, Huang, Qing, additional, Huber, Samuel, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hwang, William Y. K., additional, Iannacone, Matteo, additional, Ivison, Sabine M., additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Keller, Baerbel, additional, Kessler, Nina, additional, Ketelaars, Steven, additional, Knop, Laura, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kriegsmann, Katharina, additional, Kristyanto, H., additional, Krueger, Andreas, additional, Kuehne, Jenny F., additional, Kunze‐Schumacher, Heike, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Latorre, Daniela, additional, Lenz, Daniel, additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Long, Heather M., additional, Lugli, Enrico, additional, MacDonald, Katherine N., additional, Maggi, Laura, additional, Maini, Mala K., additional, Mair, Florian, additional, Manta, Calin, additional, Manz, Rudolf Armin, additional, Mashreghi, Mir‐Farzin, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Monin, Leticia, additional, Moretta, Lorenzo, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Muñoz‐Ruiz, Miguel, additional, Muscate, Franziska, additional, Natalini, Ambra, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Niemz, Jana, additional, Almeida, Larissa Nogueira, additional, Notarbartolo, Samuele, additional, Ostendorf, Lennard, additional, Pallett, Laura J., additional, Patel, Amit A., additional, Percin, Gulce Itir, additional, Peruzzi, Giovanna, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pujol‐Autonell, Irma, additional, Pulvirenti, Nadia, additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Rhys, Hefin, additional, Rodrigo, Maria B., additional, Romagnani, Chiara, additional, Saggau, Carina, additional, Sakaguchi, Shimon, additional, Sallusto, Federica, additional, Sanderink, Lieke, additional, Sandrock, Inga, additional, Schauer, Christine, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schober, Kilian, additional, Schoen, Janina, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulz, Axel R., additional, Schulz, Sebastian, additional, Schulze, Julia, additional, Simonetti, Sonia, additional, Singh, Jeeshan, additional, Sitnik, Katarzyna M., additional, Stark, Regina, additional, Starossom, Sarah, additional, Stehle, Christina, additional, Szelinski, Franziska, additional, Tan, Leonard, additional, Tarnok, Attila, additional, Tornack, Julia, additional, Tree, Timothy I. M., additional, van Beek, Jasper J. P., additional, van de Veen, Willem, additional, van Gisbergen, Klaas, additional, Vasco, Chiara, additional, Verheyden, Nikita A., additional, von Borstel, Anouk, additional, Ward‐Hartstonge, Kirsten A., additional, Warnatz, Klaus, additional, Waskow, Claudia, additional, Wiedemann, Annika, additional, Wilharm, Anneke, additional, Wing, James, additional, Wirz, Oliver, additional, Wittner, Jens, additional, Yang, Jennie H. M., additional, and Yang, Juhao, additional
- Published
- 2021
- Full Text
- View/download PDF
6. Helios is a marker, not a driver, of human Treg stability
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Lam, Avery J., primary, Uday, Prakruti, additional, Gillies, Jana K., additional, and Levings, Megan K., additional
- Published
- 2021
- Full Text
- View/download PDF
7. The parasite cytokine mimic Hp-TGM potently replicates the regulatory effects of TGF-β on murine CD4+ T cells
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White, Madeleine P.J., Smyth, Danielle J., Cook, Laura, Ziegler, Steven F., Levings, Megan K., and Maizels, Rick M.
- Abstract
Transforming growth factor‐beta (TGF‐β) family proteins mediate many vital biological functions in growth, development and regulation of the immune system. TGF‐β itself controls immune homeostasis and inflammation, including conversion of naïve CD4+ T cells into Foxp3+ regulatory T cells (Tregs) in the presence of IL‐2 and T cell receptor ligands. The helminth parasite Heligmosomoides polygyrus exploits this pathway through a structurally novel TGF‐β mimic (Hp‐TGM), which binds to mammalian TGF‐β receptors and induces Tregs. Here, we performed detailed comparisons of Hp‐TGM with mammalian TGF‐β. Compared to TGF‐β, Hp‐TGM induced greater numbers of Foxp3+ Tregs (iTregs), with more intense Foxp3 expression. Both ligands upregulated Treg functional markers CD73, CD103 and PD‐L1, but Hp‐TGM induced significantly higher CD39 expression than did TGF‐β. Interestingly, in contrast to canonical TGF‐β signalling through Smad2/3, Hp‐TGM stimulation was slower and more sustained. Gene expression profiles induced by TGF‐β and Hp‐TGM were remarkably similar, and both types of iTregs suppressed T cell responses in vitro and EAE‐driven inflammation in vivo. In vitro, both types of iTregs were equally stable under inflammatory conditions, but Hp‐TGM‐induced iTregs were more stable in vivo during DSS‐induced colitis, with greater retention of Foxp3 expression and lower conversion to a ROR‐γt+ phenotype. Altogether, results from this study suggest that the parasite cytokine mimic, Hp‐TGM, may deliver a qualitatively different signal to CD4+ T cells with downstream consequences for the long‐term stability of iTregs. These data highlight the potential of Hp‐TGM as a new modulator of T cell responses in vitro and in vivo.
- Published
- 2021
8. Induction of stable human FOXP3+ Tregs by a parasite‐derived TGF‐β mimic
- Author
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Cook, Laura, primary, Reid, Kyle T, additional, Häkkinen, Elmeri, additional, de Bie, Brett, additional, Tanaka, Shigeru, additional, Smyth, Danielle J, additional, White, Madeleine PJ, additional, Wong, May Q, additional, Huang, Qing, additional, Gillies, Jana K, additional, Ziegler, Steven F., additional, Maizels, Rick M, additional, and Levings, Megan K, additional
- Published
- 2021
- Full Text
- View/download PDF
9. Cross talk between human regulatory T cells and antigen‐presenting cells: Lessons for clinical applications
- Author
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Wardell, Christine M, primary, MacDonald, Katherine N, additional, Levings, Megan K, additional, and Cook, Laura, additional
- Published
- 2020
- Full Text
- View/download PDF
10. Pharmacological inhibition of RORC2 enhances human Th17‐Treg stability and function
- Author
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Boardman, Dominic A., primary, Garcia, Rosa V., additional, Ivison, Sabine M., additional, Bressler, Brian, additional, Dhar, TG Murali, additional, Zhao, Qihong, additional, and Levings, Megan K., additional
- Published
- 2020
- Full Text
- View/download PDF
11. Ectopic germline recombination activity of the widely used Foxp3‐YFP‐Cre mouse: a case report
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Wu, Dan, primary, Huang, Qing, additional, Orban, Paul C., additional, and Levings, Megan K., additional
- Published
- 2019
- Full Text
- View/download PDF
12. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- Author
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Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Acs, Andreas, additional, Adam, Dieter, additional, Adam‐Klages, Sabine, additional, Agace, William W., additional, Aghaeepour, Nima, additional, Akdis, Mübeccel, additional, Allez, Matthieu, additional, Almeida, Larissa Nogueira, additional, Alvisi, Giorgia, additional, Anderson, Graham, additional, Andrä, Immanuel, additional, Annunziato, Francesco, additional, Anselmo, Achille, additional, Bacher, Petra, additional, Baldari, Cosima T., additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Battistini, Luca, additional, Bauer, Wolfgang, additional, Baumgart, Sabine, additional, Baumgarth, Nicole, additional, Baumjohann, Dirk, additional, Baying, Bianka, additional, Bebawy, Mary, additional, Becher, Burkhard, additional, Beisker, Wolfgang, additional, Benes, Vladimir, additional, Beyaert, Rudi, additional, Blanco, Alfonso, additional, Boardman, Dominic A., additional, Bogdan, Christian, additional, Borger, Jessica G., additional, Borsellino, Giovanna, additional, Boulais, Philip E., additional, Bradford, Jolene A., additional, Brenner, Dirk, additional, Brinkman, Ryan R., additional, Brooks, Anna E. S., additional, Busch, Dirk H., additional, Büscher, Martin, additional, Bushnell, Timothy P., additional, Calzetti, Federica, additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cao, Xuetao, additional, Cardell, Susanna L., additional, Casola, Stefano, additional, Cassatella, Marco A., additional, Cavani, Andrea, additional, Celada, Antonio, additional, Chatenoud, Lucienne, additional, Chattopadhyay, Pratip K., additional, Chow, Sue, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Clerici, Mario, additional, Colombo, Federico S., additional, Cook, Laura, additional, Cooke, Anne, additional, Cooper, Andrea M., additional, Corbett, Alexandra J., additional, Cosma, Antonio, additional, Cosmi, Lorenzo, additional, Coulie, Pierre G., additional, Cumano, Ana, additional, Cvetkovic, Ljiljana, additional, Dang, Van Duc, additional, Dang‐Heine, Chantip, additional, Davey, Martin S., additional, Davies, Derek, additional, De Biasi, Sara, additional, Del Zotto, Genny, additional, Dela Cruz, Gelo Victoriano, additional, Delacher, Michael, additional, Della Bella, Silvia, additional, Dellabona, Paolo, additional, Deniz, Günnur, additional, Dessing, Mark, additional, Di Santo, James P., additional, Diefenbach, Andreas, additional, Dieli, Francesco, additional, Dolf, Andreas, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dudziak, Diana, additional, Dustin, Michael, additional, Dutertre, Charles‐Antoine, additional, Ebner, Friederike, additional, Eckle, Sidonia B. G., additional, Edinger, Matthias, additional, Eede, Pascale, additional, Ehrhardt, Götz R.A., additional, Eich, Marcus, additional, Engel, Pablo, additional, Engelhardt, Britta, additional, Erdei, Anna, additional, Esser, Charlotte, additional, Everts, Bart, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Fehniger, Todd A., additional, Felipo‐Benavent, Mar, additional, Ferry, Helen, additional, Feuerer, Markus, additional, Filby, Andrew, additional, Filkor, Kata, additional, Fillatreau, Simon, additional, Follo, Marie, additional, Förster, Irmgard, additional, Foster, John, additional, Foulds, Gemma A., additional, Frehse, Britta, additional, Frenette, Paul S., additional, Frischbutter, Stefan, additional, Fritzsche, Wolfgang, additional, Galbraith, David W., additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Gaudilliere, Brice, additional, Gazzinelli, Ricardo T., additional, Geginat, Jens, additional, Gerner, Wilhelm, additional, Gherardin, Nicholas A., additional, Ghoreschi, Kamran, additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Goda, Keisuke, additional, Godfrey, Dale I., additional, Goettlinger, Christoph, additional, González‐Navajas, Jose M., additional, Goodyear, Carl S., additional, Gori, Andrea, additional, Grogan, Jane L., additional, Grummitt, Daryl, additional, Grützkau, Andreas, additional, Haftmann, Claudia, additional, Hahn, Jonas, additional, Hammad, Hamida, additional, Hämmerling, Günter, additional, Hansmann, Leo, additional, Hansson, Goran, additional, Harpur, Christopher M., additional, Hartmann, Susanne, additional, Hauser, Andrea, additional, Hauser, Anja E., additional, Haviland, David L., additional, Hedley, David, additional, Hernández, Daniela C., additional, Herrera, Guadalupe, additional, Herrmann, Martin, additional, Hess, Christoph, additional, Höfer, Thomas, additional, Hoffmann, Petra, additional, Hogquist, Kristin, additional, Holland, Tristan, additional, Höllt, Thomas, additional, Holmdahl, Rikard, additional, Hombrink, Pleun, additional, Houston, Jessica P., additional, Hoyer, Bimba F., additional, Huang, Bo, additional, Huang, Fang‐Ping, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hunter, Christopher A., additional, Hwang, William Y. K., additional, Iannone, Anna, additional, Ingelfinger, Florian, additional, Ivison, Sabine M, additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Jávega, Beatriz, additional, Jonjic, Stipan, additional, Kaiser, Toralf, additional, Kalina, Tomas, additional, Kamradt, Thomas, additional, Kaufmann, Stefan H. E., additional, Keller, Baerbel, additional, Ketelaars, Steven L. C., additional, Khalilnezhad, Ahad, additional, Khan, Srijit, additional, Kisielow, Jan, additional, Klenerman, Paul, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kolls, Jay K., additional, Kong, Wan Ting, additional, Kopf, Manfred, additional, Korn, Thomas, additional, Kriegsmann, Katharina, additional, Kristyanto, Hendy, additional, Kroneis, Thomas, additional, Krueger, Andreas, additional, Kühne, Jenny, additional, Kukat, Christian, additional, Kunkel, Désirée, additional, Kunze‐Schumacher, Heike, additional, Kurosaki, Tomohiro, additional, Kurts, Christian, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Landry, Jonathan, additional, Lantz, Olivier, additional, Lanuti, Paola, additional, LaRosa, Francesca, additional, Lehuen, Agnès, additional, LeibundGut‐Landmann, Salomé, additional, Leipold, Michael D., additional, Leung, Leslie Y.T., additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Litwin, Virginia, additional, Liu, Yanling, additional, Ljunggren, Hans‐Gustaf, additional, Lohoff, Michael, additional, Lombardi, Giovanna, additional, Lopez, Lilly, additional, López‐Botet, Miguel, additional, Lovett‐Racke, Amy E., additional, Lubberts, Erik, additional, Luche, Herve, additional, Ludewig, Burkhard, additional, Lugli, Enrico, additional, Lunemann, Sebastian, additional, Maecker, Holden T., additional, Maggi, Laura, additional, Maguire, Orla, additional, Mair, Florian, additional, Mair, Kerstin H., additional, Mantovani, Alberto, additional, Manz, Rudolf A., additional, Marshall, Aaron J., additional, Martínez‐Romero, Alicia, additional, Martrus, Glòria, additional, Marventano, Ivana, additional, Maslinski, Wlodzimierz, additional, Matarese, Giuseppe, additional, Mattioli, Anna Vittoria, additional, Maueröder, Christian, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, McGrath, Mairi, additional, McGuire, Helen M., additional, McInnes, Iain B., additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Miller, Stephen D., additional, Mills, Kingston H.G., additional, Minderman, Hans, additional, Mjösberg, Jenny, additional, Moore, Jonni, additional, Moran, Barry, additional, Moretta, Lorenzo, additional, Mosmann, Tim R., additional, Müller, Susann, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Münz, Christian, additional, Nakayama, Toshinori, additional, Nasi, Milena, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Nourshargh, Sussan, additional, Núñez, Gabriel, additional, O'Connor, José‐Enrique, additional, Ochel, Aaron, additional, Oja, Anna, additional, Ordonez, Diana, additional, Orfao, Alberto, additional, Orlowski‐Oliver, Eva, additional, Ouyang, Wenjun, additional, Oxenius, Annette, additional, Palankar, Raghavendra, additional, Panse, Isabel, additional, Pattanapanyasat, Kovit, additional, Paulsen, Malte, additional, Pavlinic, Dinko, additional, Penter, Livius, additional, Peterson, Pärt, additional, Peth, Christian, additional, Petriz, Jordi, additional, Piancone, Federica, additional, Pickl, Winfried F., additional, Piconese, Silvia, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Podolska, Malgorzata Justyna, additional, Poon, Zhiyong, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pucillo, Carlo E. M., additional, Quataert, Sally A., additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Radstake, Tim R. D. J., additional, Rahmig, Susann, additional, Rahn, Hans‐Peter, additional, Rajwa, Bartek, additional, Ravichandran, Gevitha, additional, Raz, Yotam, additional, Rebhahn, Jonathan A., additional, Recktenwald, Diether, additional, Reimer, Dorothea, additional, Reis e Sousa, Caetano, additional, Remmerswaal, Ester B.M., additional, Richter, Lisa, additional, Rico, Laura G., additional, Riddell, Andy, additional, Rieger, Aja M., additional, Robinson, J. Paul, additional, Romagnani, Chiara, additional, Rubartelli, Anna, additional, Ruland, Jürgen, additional, Saalmüller, Armin, additional, Saeys, Yvan, additional, Saito, Takashi, additional, Sakaguchi, Shimon, additional, Sala‐de‐Oyanguren, Francisco, additional, Samstag, Yvonne, additional, Sanderson, Sharon, additional, Sandrock, Inga, additional, Santoni, Angela, additional, Sanz, Ramon Bellmàs, additional, Saresella, Marina, additional, Sautes‐Fridman, Catherine, additional, Sawitzki, Birgit, additional, Schadt, Linda, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schimisky, Esther, additional, Schlitzer, Andreas, additional, Schlosser, Josephine, additional, Schmid, Stephan, additional, Schmitt, Steffen, additional, Schober, Kilian, additional, Schraivogel, Daniel, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulte, Reiner, additional, Schulz, Axel Ronald, additional, Schulz, Sebastian R., additional, Scottá, Cristiano, additional, Scott‐Algara, Daniel, additional, Sester, David P., additional, Shankey, T. Vincent, additional, Silva‐Santos, Bruno, additional, Simon, Anna Katharina, additional, Sitnik, Katarzyna M., additional, Sozzani, Silvano, additional, Speiser, Daniel E., additional, Spidlen, Josef, additional, Stahlberg, Anders, additional, Stall, Alan M., additional, Stanley, Natalie, additional, Stark, Regina, additional, Stehle, Christina, additional, Steinmetz, Tobit, additional, Stockinger, Hannes, additional, Takahama, Yousuke, additional, Takeda, Kiyoshi, additional, Tan, Leonard, additional, Tárnok, Attila, additional, Tiegs, Gisa, additional, Toldi, Gergely, additional, Tornack, Julia, additional, Traggiai, Elisabetta, additional, Trebak, Mohamed, additional, Tree, Timothy I.M., additional, Trotter, Joe, additional, Trowsdale, John, additional, Tsoumakidou, Maria, additional, Ulrich, Henning, additional, Urbanczyk, Sophia, additional, van de Veen, Willem, additional, van den Broek, Maries, additional, van der Pol, Edwin, additional, Van Gassen, Sofie, additional, Van Isterdael, Gert, additional, van Lier, René A.W., additional, Veldhoen, Marc, additional, Vento‐Asturias, Salvador, additional, Vieira, Paulo, additional, Voehringer, David, additional, Volk, Hans‐Dieter, additional, von Borstel, Anouk, additional, von Volkmann, Konrad, additional, Waisman, Ari, additional, Walker, Rachael V., additional, Wallace, Paul K., additional, Wang, Sa A., additional, Wang, Xin M., additional, Ward, Michael D., additional, Ward‐Hartstonge, Kirsten A, additional, Warnatz, Klaus, additional, Warnes, Gary, additional, Warth, Sarah, additional, Waskow, Claudia, additional, Watson, James V., additional, Watzl, Carsten, additional, Wegener, Leonie, additional, Weisenburger, Thomas, additional, Wiedemann, Annika, additional, Wienands, Jürgen, additional, Wilharm, Anneke, additional, Wilkinson, Robert John, additional, Willimsky, Gerald, additional, Wing, James B., additional, Winkelmann, Rieke, additional, Winkler, Thomas H., additional, Wirz, Oliver F., additional, Wong, Alicia, additional, Wurst, Peter, additional, Yang, Jennie H. M., additional, Yang, Juhao, additional, Yazdanbakhsh, Maria, additional, Yu, Liping, additional, Yue, Alice, additional, Zhang, Hanlin, additional, Zhao, Yi, additional, Ziegler, Susanne Maria, additional, Zielinski, Christina, additional, Zimmermann, Jakob, additional, and Zychlinsky, Arturo, additional
- Published
- 2019
- Full Text
- View/download PDF
13. Characterization of regulatory T cells in obese omental adipose tissue in humans
- Author
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Wu, Dan, primary, Han, Jonathan M., additional, Yu, Xin, additional, Lam, Avery J., additional, Hoeppli, Romy E., additional, Pesenacker, Anne M., additional, Huang, Qing, additional, Chen, Virginia, additional, Speake, Cate, additional, Yorke, Ekua, additional, Nguyen, Nam, additional, Sampath, Sharadh, additional, Harris, David, additional, and Levings, Megan K., additional
- Published
- 2019
- Full Text
- View/download PDF
14. An optimized method to measure human FOXP3+ regulatory T cells from multiple tissue types using mass cytometry
- Author
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Dawson, Nicholas A. J., primary, Lam, Avery J., additional, Cook, Laura, additional, Hoeppli, Romy E., additional, Broady, Raewyn, additional, Pesenacker, Anne M., additional, and Levings, Megan K., additional
- Published
- 2018
- Full Text
- View/download PDF
15. Guidelines for the use of flow cytometry and cell sorting in immunological studies *
- Author
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Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Akdis, Mübeccel, additional, Andrä, Immanuel, additional, Annunziato, Francesco, additional, Bacher, Petra, additional, Barnaba, Vincenzo, additional, Battistini, Luca, additional, Bauer, Wolfgang M., additional, Baumgart, Sabine, additional, Becher, Burkhard, additional, Beisker, Wolfgang, additional, Berek, Claudia, additional, Blanco, Alfonso, additional, Borsellino, Giovanna, additional, Boulais, Philip E., additional, Brinkman, Ryan R., additional, Büscher, Martin, additional, Busch, Dirk H., additional, Bushnell, Timothy P., additional, Cao, Xuetao, additional, Cavani, Andrea, additional, Chattopadhyay, Pratip K., additional, Cheng, Qingyu, additional, Chow, Sue, additional, Clerici, Mario, additional, Cooke, Anne, additional, Cosma, Antonio, additional, Cosmi, Lorenzo, additional, Cumano, Ana, additional, Dang, Van Duc, additional, Davies, Derek, additional, Biasi, Sara, additional, Del Zotto, Genny, additional, Della Bella, Silvia, additional, Dellabona, Paolo, additional, Deniz, Günnur, additional, Dessing, Mark, additional, Diefenbach, Andreas, additional, Di Santo, James, additional, Dieli, Francesco, additional, Dolf, Andreas, additional, Donnenberg, Vera S., additional, Dörner, Thomas, additional, Ehrhardt, Götz R. A., additional, Endl, Elmar, additional, Engel, Pablo, additional, Engelhardt, Britta, additional, Esser, Charlotte, additional, Everts, Bart, additional, Dreher, Anita, additional, Falk, Christine S., additional, Fehniger, Todd A., additional, Filby, Andrew, additional, Fillatreau, Simon, additional, Follo, Marie, additional, Förster, Irmgard, additional, Foster, John, additional, Foulds, Gemma A., additional, Frenette, Paul S., additional, Galbraith, David, additional, Garbi, Natalio, additional, García‐Godoy, Maria Dolores, additional, Geginat, Jens, additional, Ghoreschi, Kamran, additional, Gibellini, Lara, additional, Goettlinger, Christoph, additional, Goodyear, Carl S., additional, Gori, Andrea, additional, Grogan, Jane, additional, Gross, Mor, additional, Grützkau, Andreas, additional, Grummitt, Daryl, additional, Hahn, Jonas, additional, Hammer, Quirin, additional, Hauser, Anja E., additional, Haviland, David L., additional, Hedley, David, additional, Herrera, Guadalupe, additional, Herrmann, Martin, additional, Hiepe, Falk, additional, Holland, Tristan, additional, Hombrink, Pleun, additional, Houston, Jessica P., additional, Hoyer, Bimba F., additional, Huang, Bo, additional, Hunter, Christopher A., additional, Iannone, Anna, additional, Jäck, Hans‐Martin, additional, Jávega, Beatriz, additional, Jonjic, Stipan, additional, Juelke, Kerstin, additional, Jung, Steffen, additional, Kaiser, Toralf, additional, Kalina, Tomas, additional, Keller, Baerbel, additional, Khan, Srijit, additional, Kienhöfer, Deborah, additional, Kroneis, Thomas, additional, Kunkel, Désirée, additional, Kurts, Christian, additional, Kvistborg, Pia, additional, Lannigan, Joanne, additional, Lantz, Olivier, additional, Larbi, Anis, additional, LeibundGut‐Landmann, Salome, additional, Leipold, Michael D., additional, Levings, Megan K., additional, Litwin, Virginia, additional, Liu, Yanling, additional, Lohoff, Michael, additional, Lombardi, Giovanna, additional, Lopez, Lilly, additional, Lovett‐Racke, Amy, additional, Lubberts, Erik, additional, Ludewig, Burkhard, additional, Lugli, Enrico, additional, Maecker, Holden T., additional, Martrus, Glòria, additional, Matarese, Giuseppe, additional, Maueröder, Christian, additional, McGrath, Mairi, additional, McInnes, Iain, additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Mills, Kingston, additional, Mirrer, David, additional, Mjösberg, Jenny, additional, Moore, Jonni, additional, Moran, Barry, additional, Moretta, Alessandro, additional, Moretta, Lorenzo, additional, Mosmann, Tim R., additional, Müller, Susann, additional, Müller, Werner, additional, Münz, Christian, additional, Multhoff, Gabriele, additional, Munoz, Luis Enrique, additional, Murphy, Kenneth M., additional, Nakayama, Toshinori, additional, Nasi, Milena, additional, Neudörfl, Christine, additional, Nolan, John, additional, Nourshargh, Sussan, additional, O'Connor, José‐Enrique, additional, Ouyang, Wenjun, additional, Oxenius, Annette, additional, Palankar, Raghav, additional, Panse, Isabel, additional, Peterson, Pärt, additional, Peth, Christian, additional, Petriz, Jordi, additional, Philips, Daisy, additional, Pickl, Winfried, additional, Piconese, Silvia, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Podolska, Malgorzata Justyna, additional, Pucillo, Carlo, additional, Quataert, Sally A., additional, Radstake, Timothy R. D. J., additional, Rajwa, Bartek, additional, Rebhahn, Jonathan A., additional, Recktenwald, Diether, additional, Remmerswaal, Ester B.M., additional, Rezvani, Katy, additional, Rico, Laura G., additional, Robinson, J. Paul, additional, Romagnani, Chiara, additional, Rubartelli, Anna, additional, Ruckert, Beate, additional, Ruland, Jürgen, additional, Sakaguchi, Shimon, additional, Sala‐de‐Oyanguren, Francisco, additional, Samstag, Yvonne, additional, Sanderson, Sharon, additional, Sawitzki, Birgit, additional, Scheffold, Alexander, additional, Schiemann, Matthias, additional, Schildberg, Frank, additional, Schimisky, Esther, additional, Schmid, Stephan A, additional, Schmitt, Steffen, additional, Schober, Kilian, additional, Schüler, Thomas, additional, Schulz, Axel Ronald, additional, Schumacher, Ton, additional, Scotta, Cristiano, additional, Shankey, T. Vincent, additional, Shemer, Anat, additional, Simon, Anna‐Katharina, additional, Spidlen, Josef, additional, Stall, Alan M., additional, Stark, Regina, additional, Stehle, Christina, additional, Stein, Merle, additional, Steinmetz, Tobit, additional, Stockinger, Hannes, additional, Takahama, Yousuke, additional, Tarnok, Attila, additional, Tian, ZhiGang, additional, Toldi, Gergely, additional, Tornack, Julia, additional, Traggiai, Elisabetta, additional, Trotter, Joe, additional, Ulrich, Henning, additional, der Braber, Marlous, additional, Lier, René A. W., additional, Veldhoen, Marc, additional, Vento‐Asturias, Salvador, additional, Vieira, Paulo, additional, Voehringer, David, additional, Volk, Hans‐Dieter, additional, Volkmann, Konrad, additional, Waisman, Ari, additional, Walker, Rachael, additional, Ward, Michael D., additional, Warnatz, Klaus, additional, Warth, Sarah, additional, Watson, James V., additional, Watzl, Carsten, additional, Wegener, Leonie, additional, Wiedemann, Annika, additional, Wienands, Jürgen, additional, Willimsky, Gerald, additional, Wing, James, additional, Wurst, Peter, additional, Yu, Liping, additional, Yue, Alice, additional, Zhang, Qianjun, additional, Zhao, Yi, additional, Ziegler, Susanne, additional, and Zimmermann, Jakob, additional
- Published
- 2017
- Full Text
- View/download PDF
16. Adipose-tissue regulatory T cells: Critical players in adipose-immune crosstalk
- Author
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Becker, Maike, primary, Levings, Megan K., additional, and Daniel, Carolin, additional
- Published
- 2017
- Full Text
- View/download PDF
17. Control of tissue‐localized immune responses by human regulatory T cells
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Pesenacker, Anne M., primary, Broady, Raewyn, additional, and Levings, Megan K., additional
- Published
- 2014
- Full Text
- View/download PDF
18. ATP conditions intestinal epithelial cells to an inflammatory state that promotes components of DC maturation
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Yao, Yu, primary, Levings, Megan K., additional, and Steiner, Theodore S., additional
- Published
- 2012
- Full Text
- View/download PDF
19. Regulatory T-cell therapy for inflammatory bowel disease: more questions than answers
- Author
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Himmel, Megan E., primary, Yao, Yu, additional, Orban, Paul C., additional, Steiner, Theodore S., additional, and Levings, Megan K., additional
- Published
- 2012
- Full Text
- View/download PDF
20. Suppression assays with human T regulatory cells: A technical guide
- Author
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McMurchy, Alicia N., primary and Levings, Megan K., additional
- Published
- 2011
- Full Text
- View/download PDF
21. CD4+Foxp3+ regulatory T cells suppress γδ T-cell effector functions in a model of T-cell-induced mucosal inflammation
- Author
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Yurchenko, Ekaterina, primary, Levings, Megan K., additional, and Piccirillo, Ciriaco A., additional
- Published
- 2011
- Full Text
- View/download PDF
22. Human CD4+FOXP3+ regulatory T cells produce CXCL8 and recruit neutrophils
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Himmel, Megan E., primary, Crome, Sarah Q., additional, Ivison, Sabine, additional, Piccirillo, Ciriaco, additional, Steiner, Theodore S., additional, and Levings, Megan K., additional
- Published
- 2011
- Full Text
- View/download PDF
23. Natural killer T cells constitutively expressing the interleukin-2 receptor α chain early in life are primed to respond to lower antigenic stimulation
- Author
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Ladd, Mihoko, primary, Sharma, Ashish, additional, Huang, Qing, additional, Wang, Adele Y., additional, Xu, Lixin, additional, Genowati, Indira, additional, Levings, Megan K., additional, and Lavoie, Pascal M., additional
- Published
- 2010
- Full Text
- View/download PDF
24. CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC
- Author
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Maggi, Laura, primary, Santarlasci, Veronica, additional, Capone, Manuela, additional, Peired, Anna, additional, Frosali, Francesca, additional, Crome, Sarah Q., additional, Querci, Valentina, additional, Fambrini, Massimiliano, additional, Liotta, Francesco, additional, Levings, Megan K., additional, Maggi, Enrico, additional, Cosmi, Lorenzo, additional, Romagnani, Sergio, additional, and Annunziato, Francesco, additional
- Published
- 2010
- Full Text
- View/download PDF
25. The role of retinoic acid‐related orphan receptor variant 2 and IL‐17 in the development and function of human CD4+ T cells
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Crome, Sarah Q., primary, Wang, Adele Y., additional, Kang, Christine Y., additional, and Levings, Megan K., additional
- Published
- 2009
- Full Text
- View/download PDF
26. Inducible reprogramming of human T cells into Treg cells by a conditionally active form of FOXP3
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Allan, Sarah E., primary, Song-Zhao, George X., additional, Abraham, Thomas, additional, McMurchy, Alicia N., additional, and Levings, Megan K., additional
- Published
- 2008
- Full Text
- View/download PDF
27. The role of T-regulatory cells and Toll-like receptors in the pathogenesis of human inflammatory bowel disease
- Author
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Himmel, Megan E., primary, Hardenberg, Gijs, additional, Piccirillo, Ciriaco A., additional, Steiner, Theodore S., additional, and Levings, Megan K., additional
- Published
- 2008
- Full Text
- View/download PDF
28. CD4+T-regulatory cells: toward therapy for human diseases
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Allan, Sarah E., primary, Broady, Raewyn, additional, Gregori, Silvia, additional, Himmel, Megan E., additional, Locke, Natasha, additional, Roncarolo, Maria Grazia, additional, Bacchetta, Rosa, additional, and Levings, Megan K., additional
- Published
- 2008
- Full Text
- View/download PDF
29. Pro-tolerogenic effects of photodynamic therapy with TH9402 on dendritic cells
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Broady, Raewyn, primary, Yu, Jie, additional, and Levings, Megan K., additional
- Published
- 2008
- Full Text
- View/download PDF
30. Interleukin‐10‐secreting type 1 regulatory T cells in rodents and humans
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Grazia Roncarolo, Maria, primary, Gregori, Silvia, additional, Battaglia, Manuela, additional, Bacchetta, Rosa, additional, Fleischhauer, Katharina, additional, and Levings, Megan K., additional
- Published
- 2006
- Full Text
- View/download PDF
31. Can we throw the master-switch in autoimmune disease?
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Orban, Paul C, primary and Levings, Megan K, additional
- Published
- 2003
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32. Discoveries in sphingolipid metabolism, spinocerebellar ataxia and autoimmune disease
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Brunham, Liam R, primary, Chan, Edmond YW, additional, Orban, Paul C, additional, and Levings, Megan K, additional
- Published
- 2003
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- View/download PDF
33. Type 1 T regulatory cells
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Roncarolo, Maria Grazia, primary, Bacchetta, Rosa, additional, Bordignon, Claudio, additional, Narula, Satwant, additional, and Levings, Megan K., additional
- Published
- 2001
- Full Text
- View/download PDF
34. Induction of stable human FOXP3 + Tregs by a parasite-derived TGF-β mimic.
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Cook L, Reid KT, Häkkinen E, de Bie B, Tanaka S, Smyth DJ, White MP, Wong MQ, Huang Q, Gillies JK, Ziegler SF, Maizels RM, and Levings MK
- Subjects
- Animals, Forkhead Transcription Factors, Humans, T-Lymphocytes, Regulatory, Th17 Cells, Transforming Growth Factor beta, Parasites
- Abstract
Immune homeostasis in the intestine is tightly controlled by FOXP3
+ regulatory T cells (Tregs), defects of which are linked to the development of chronic conditions, such as inflammatory bowel disease (IBD). As a mechanism of immune evasion, several species of intestinal parasites boost Treg activity. The parasite Heligmosomoides polygyrus is known to secrete a molecule (Hp-TGM) that mimics the ability of TGF-β to induce FOXP3 expression in CD4+ T cells. The study aimed to investigate whether Hp-TGM could induce human FOXP3+ Tregs as a potential therapeutic approach for inflammatory diseases. CD4+ T cells from healthy volunteers were expanded in the presence of Hp-TGM or TGF-β. Treg induction was measured by flow cytometric detection of FOXP3 and other Treg markers, such as CD25 and CTLA-4. Epigenetic changes were detected using ChIP-Seq and pyrosequencing of FOXP3. Treg phenotype stability was assessed following inflammatory cytokine challenge and Treg function was evaluated by cellular co-culture suppression assays and cytometric bead arrays for secreted cytokines. Hp-TGM efficiently induced FOXP3 expression (> 60%), in addition to CD25 and CTLA-4, and caused epigenetic modification of the FOXP3 locus to a greater extent than TGF-β. Hp-TGM-induced Tregs had superior suppressive function compared with TGF-β-induced Tregs, and retained their phenotype following exposure to inflammatory cytokines. Furthermore, Hp-TGM induced a Treg-like phenotype in in vivo differentiated Th1 and Th17 cells, indicating its potential to re-program memory cells to enhance immune tolerance. These data indicate Hp-TGM has potential to be used to generate stable human FOXP3+ Tregs to treat IBD and other inflammatory diseases., (© 2021 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.)- Published
- 2021
- Full Text
- View/download PDF
35. The parasite cytokine mimic Hp-TGM potently replicates the regulatory effects of TGF-β on murine CD4 + T cells.
- Author
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White MPJ, Smyth DJ, Cook L, Ziegler SF, Levings MK, and Maizels RM
- Subjects
- Animals, Cytokines, Forkhead Transcription Factors, Mice, T-Lymphocytes, Regulatory, Parasites, Transforming Growth Factor beta
- Abstract
Transforming growth factor-beta (TGF-β) family proteins mediate many vital biological functions in growth, development and regulation of the immune system. TGF-β itself controls immune homeostasis and inflammation, including conversion of naïve CD4
+ T cells into Foxp3+ regulatory T cells (Tregs) in the presence of interleukin-2 and T-cell receptor ligands. The helminth parasite Heligmosomoides polygyrus exploits this pathway through a structurally novel TGF-β mimic (Hp-TGM), which binds to mammalian TGF-β receptors and induces Tregs. Here, we performed detailed comparisons of Hp-TGM with mammalian TGF-β. Compared with TGF-β, Hp-TGM induced greater numbers of Foxp3+ Tregs (iTregs), with more intense Foxp3 expression. Both ligands upregulated Treg functional markers CD73, CD103 and programmed death-ligand 1, but Hp-TGM induced significantly higher CD39 expression than did TGF-β. Interestingly, in contrast to canonical TGF-β signaling through Smad2/3, Hp-TGM stimulation was slower and more sustained. Gene expression profiles induced by TGF-β and Hp-TGM were remarkably similar, and both types of iTregs suppressed T-cell responses in vitro and experimental autoimmune encephalomyelitis-driven inflammation in vivo. In vitro, both types of iTregs were equally stable under inflammatory conditions, but Hp-TGM-induced iTregs were more stable in vivo during dextran sodium sulfate-induced colitis, with greater retention of Foxp3 expression and lower conversion to a ROR-γt+ phenotype. Altogether, results from this study suggest that the parasite cytokine mimic, Hp-TGM, may deliver a qualitatively different signal to CD4+ T cells with downstream consequences for the long-term stability of iTregs. These data highlight the potential of Hp-TGM as a new modulator of T-cell responses in vitro and in vivo., (© 2021 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.)- Published
- 2021
- Full Text
- View/download PDF
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