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Your search keyword '"Li, Jun-Ming"' showing total 12 results
Start Over Author "Li, Jun-Ming" Publisher wiley
12 results on '"Li, Jun-Ming"'

1. Biotoxicity of Halide Perovskites in Mice

Author

Li, Guixiang, primary, Liu, Yong‐Tao, additional, Yang, Feng, additional, Li, Meng, additional, Zhang, Zuhong, additional, Pascual, Jorge, additional, Wang, Zhao‐Kui, additional, Wei, Shi‐Zhe, additional, Zhao, Xin‐Yuan, additional, Liu, Hai‐Rui, additional, Zhao, Jin‐Bo, additional, Lin, Chieh‐Ting, additional, Li, Jun‐Ming, additional, Li, Zhe, additional, Abate, Antonio, additional, and Cantone, Irene, additional

Published
2023
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12. Evaluation of embryonic age and the effects of different proteases on the isolation and primary culture of chicken intestinal epithelial cells in vitro.

Author

Yuan C, He Q, Li JM, Azzam MM, Lu JJ, and Zou XT

Subjects
Animals, Cell Proliferation, Cells, Cultured, Chick Embryo, Chickens, Culture Media, Edetic Acid pharmacology, Embryonic Development, Intestinal Mucosa enzymology, Thermolysin metabolism, Trypsin pharmacology, Cell Culture Techniques methods, Cell Separation methods, Collagenases metabolism, Enterocytes cytology, Intestinal Mucosa cytology, Intestinal Mucosa embryology
Abstract

The present study evaluates the effects of embryonic age and proteolytic enzymes on the isolation and primary culture of chicken enterocyte and to establish an effective technique for chicken intestinal epithelial cell (IEC) cultivation. Fourteen-day-old, 16-day-old and 18-day-old embryos (average weight: 52.23 ± 0.76 g, 50.86 ± 0.99 g, 48.98 ± 1.03 g) were the source for preparation of enterocyte culture, and trypsin-ethylene diamine tetraacetic acid, collagenase, thermolysin and combination of collagenase and thermolysin were used for digestion medium. Optimal culture protocols were determined by qualitative assays of proliferation. Cells isolated by using 14-day-old embryo and collagenase obtain the best attachment and growth in culture, and the production of continuously growing IEC cultures. Thus, we conclude that the use of collagenase as a dissociating enzyme and 14-day-old embryo as a source can be advantageously applied to the isolation of chicken IEC and this method may be useful for various applications and basic studies of the intestinal tract concerning such objects as physiology, immunology and toxicology., (© 2014 Japanese Society of Animal Science.)

Published
2015
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