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30 results on '"Liu, Junyan"'

18. Aberration of CCR7+ CD8+ memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4+ T cells

Author

Hu Chunsong, Yang Sen, Zhang Qiuping, Li Qun, Xuejun Zhang, Huang Baojun, Jiang San, Tan Jinquan, Liu Junyan, and Zhang Lin-jie

Subjects
Adult, Male, Receptors, CCR7, Adolescent, Immunology, Gene Expression, chemical and pharmacologic phenomena, CD8-Positive T-Lymphocytes, Biology, CCL5, Autoimmune Diseases, Immunophenotyping, Interleukin 21, Th2 Cells, T-Lymphocyte Subsets, immune system diseases, Humans, Lupus Erythematosus, Systemic, Immunology and Allergy, Cytotoxic T cell, RNA, Messenger, IL-2 receptor, Antigen-presenting cell, Reverse Transcriptase Polymerase Chain Reaction, ZAP70, hemic and immune systems, Original Articles, Middle Aged, Natural killer T cell, Chemotaxis, Leukocyte, Interleukin 12, Leukocyte Common Antigens, Female, Receptors, Chemokine, Immunologic Memory
Abstract

Chemokine receptors are important in the entry of leucocytes into the inflammatory sites of systemic lupus erythematosus (SLE). CCR7(+) and CCR7(-) memory T cells exert different functions in homing, cytokine production and cytotoxicity. To determine whether differential expression and functions of the CCR7 occur in SLE patients, we examined CCR3, CCR4, CCR5, CCR7 and CCR9 on CD4(+) and CD8(+) T cells from normal and SLE subjects. Flow cytometry, real-time quantitative reverse transcription polymerase chain reactions and Northern blotting were used to detect the expression of chemokine receptors and cytokines; a chemotaxis assay was used to detect their functions. CD4(+) T-cell stimulation with syngeneic CCR7(+) CD8(+) CD45RO(+) T cells and dendritic cells (including transwell chambers) was used to induce cytokine expression. We demonstrated that CCR7 was selectively, frequently and functionally expressed on CD8(+) (94.8%) but not on CD4(+) (16.1%) T cells from patients with active SLE, whereas this phenomenon was not seen in normal subjects and in those whose SLE was inactive. CCR7(+) CD8(+) CD45RO(+) memory T cells from patients with active SLE, themselves T helper type 2 (Th2) biased, were inducers of Th2 bias in CD4(+) T cells in a cell-cell contact manner in vitro, meanwhile, the cells from both normal subjects and those whose SLE was inactive drove CD4(+) T cells into a regulatory T-cell-derived cytokine pattern. Our findings might provide new clues to understanding the functions of CCR7(+) CD8(+) CD45RO(+)'central' memory T cells in autoimmune diseases (such as SLE). We suggest that in the case of active SLE, CCR7(+) central memory T cells were able to enter peripheral blood and inflammatory sites from secondary lymphoid organs, were continuously expressing CCR7, and interacted with dendritic cells and functioned as CCR7(-)'effector' memory T cells, which were described in normal humans.

Published
2004

19. Highly up-regulated CXCR3 expression on eosinophils in mice infected with Schistosoma japonicum

Author

Liu Junyan, Hu Chunsong, Li Qun, He Li, Zhang Qiuping, Tan Jinquan, Cai Guobin, Huang Baojun, Jiang Mingshen, Zhang Xiaolian, and Zhang Lin-jie

Subjects
Chemokine, Receptors, CXCR3, Liver Diseases, Parasitic, Immunology, CCR3, Biology, CXCR3, Schistosoma japonicum, Mice, Chemokine receptor, Animals, Ascitic Fluid, Immunology and Allergy, CXCL10, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Original Articles, Flow Cytometry, biology.organism_classification, Molecular biology, Eosinophilic Granuloma, Eosinophils, Mice, Inbred C57BL, Monokine, Chemotaxis, Leukocyte, Liver, Schistosomiasis japonica, biology.protein, CXCL9, Receptors, Chemokine
Abstract

CXCR3, predominately expressed on memory/activated T cells, is a receptor for both interferon-gamma inducible protein-10/CXC ligand 10 (CXCL10) and monokine induced by interferon-gamma/CXCL9. We reported here that CXCR3 was highly up-regulated on infiltrating eosinophils in Schistosoma japonicum egg-induced granuloma in the mouse liver. It was also highly and functionally up-regulated on peritoneal exudate eosinophils in mice infected with S. japonicum. The phenomena were demonstrated at protein and mRNA levels using immunohisto- and immunocytochemistry evaluation of biopsy, flow cytometry and real-time quantitative reverse transcriptase-polymerase chain reaction technique, and verified by Northern blotting and chemotaxis assay in vitro. We also found that CCR3 expression on the infiltrating and peritoneal exudate cells was significantly decreased, CXCR4 expression was unchanged during the 42-day period of infection. We screened mRNA expression levels of the all known chemokine receptors in purified peritoneal exudate eosinophils and liver granuloma dominated by eosinophils. CXCR3 was highly and functionally up-regulated on peritoneal exudate eosinophils in mice infected with S. japonicum, meanwhile CCR3 was significantly and functionally down-regulated in these cells. The findings could lead to a better understanding of the chemokine receptor expression pattern of eosinophils at inflamed tissue sites caused by parasites. These could be also crucial for establishing a therapeutic strategy for eosinophilic inflammation via intervention in chemokine actions.

Published
2004

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