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Your search keyword '"Liu, Wenzhi"' showing total 22 results
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22 results on '"Liu, Wenzhi"'

8. Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

Author

Xue Mingyang, Zhong Li, Yan Meng, Li Yiqun, Lingbing Zeng, Nan Jiang, Yuding Fan, Yong Zhou, and Liu Wenzhi

Subjects
China, Eels, food.ingredient, medicine.diagnostic_test, Veterinary (miscellaneous), Perhabdovirus, Aquatic Science, Biology, Kidney, biology.organism_classification, Molecular biology, Virus, Cell Line, Reverse transcription polymerase chain reaction, Fish Diseases, food, Viral replication, Cell culture, medicine, Animals, Rhabdoviridae, In Situ Hybridization, Fluorescence, Fluorescence in situ hybridization, Monopterus, Cytopathic effect
Abstract

Chinese rice-field eels rhabdovirus (CrERV), belonging to the genus Perhabdovirus in the family Rhabdoviridae, is the causative agent of the haemorrhagic disease of Chinese rice-field eels, Monopterus albus. The present study aims to establish a cell line derived from the kidney of Chinese rice-field eel (CrEK) for the further study of the pathogenic virus. CrEK cells were epithelioid-like and grew well in M199 medium supplemented with 10% foetal bovine serum at 28°C, and the cell line has been subcultured for more than 80 times. Karyotyping analysis of CrEK cells at 25th passage indicated a modal chromosome number of 24. Significant cytopathic effect (CPE) was observed in CrEK cells after infection with CrERV, and the virus titre reached 107.8 ± 0.45 TCID50 /mL. The transmission electron microscopy revealed that there were a large number of virus particles in the cytoplasm of cells. The virus infection in cells was also assayed by using indirect immunofluorescence assay (IFA), fluorescence in situ hybridization (FISH), reverse transcription PCR (RT-PCR) and quantitative real-time reverse transcription-PCR (qRT-PCR). In experimental infection, CrERV cultured by cells could cause over 90% mortality in fish. CrEK represents the first kidney cell line originated from Chinese rice-field eels and be a potential material for investigating the mechanism of virus infection in this fish and the control methods for the disease.

Published
2021

10. Comparison of concurrent chemoradiotherapy with radiotherapy alone for locally advanced esophageal squamous cell cancer in elderly patients: A randomized, multicenter, phase II clinical trial

Author

Liu, Yanxiao, primary, Zheng, Zhiyong, additional, Li, Minghao, additional, Zhang, Yaowen, additional, Zhao, Fujun, additional, Gong, Heyi, additional, Lin, Haiqun, additional, Huang, Wei, additional, Chen, Xiangming, additional, Xu, Zhiqiao, additional, Li, Xiaomin, additional, Liu, Wenzhi, additional, Cui, Yanhui, additional, Zheng, Anping, additional, and Li, Baosheng, additional

Published
2022
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22. LONGITUDINAL DISPERSION IN RWERS: A DEAD-ZONE MODEL SOLUTION

Author

Yun-Sheng Yu and Liu Wenzhi

Subjects
Hydrology, Ecology, Laplace transform, Turbulence, Inversion (meteorology), Model parameters, Dead zone, Mechanics, Dispersion coefficient, Coupled differential equations, TRACER, Geology, Earth-Surface Processes, Water Science and Technology
Abstract

The longitudinal dispersion of tracer material in rivers is simulated by a dead-zone model that involves two coupled differential equations. For an instantaneous injection of tracer material, the problem is solved by the Laplace. Transform and numerical inversion to obtain the time-concentration curve at any sampling station. The optimal values of the model parameters are estimated from field measurements. Applications of the model to three different rivers generally show that the model reproduces the longitudinal dispersion characteristics in natural streams with sufficient accuracy.

Published
1989

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