1. Protein Encapsulation in Biodegradable Polymeric Nanoparticles: Morphology, Fluorescence Behaviour and Stem Cell Uptake
- Author
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Luigi Tarpani, Ilaria Armentano, Nicoletta Rescignano, Simona Montesano, Loredana Latterini, Jose Maria Kenny, Sabata Martino, and Roberto Tiribuzi
- Subjects
Polymers and Plastics ,biology ,Chemistry ,education ,technology, industry, and agriculture ,Nanoparticle ,Bioengineering ,Polymeric nanoparticles ,Double emulsion ,Fluorescence ,Biomaterials ,chemistry.chemical_compound ,PLGA ,Materials Chemistry ,biology.protein ,Biophysics ,Organic chemistry ,Stem cell ,Bovine serum albumin ,Fluorescein isothiocyanate ,health care economics and organizations ,Biotechnology - Abstract
The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.
- Published
- 2013
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