1. The diagnostic accuracy of two methods for E6&7 mRNA detection in women with minor cytological abnormalities
- Author
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Petros Karakitsos, Aris Spathis, Emmanuel Kountouris, Maria Alepaki, Sofia Makridima, Vicky Stathopoulou, Charalambos Chrelias, George Koliopoulos, Pericles Panagopoulos, Dimitrios Kassanos, Ioannis Panayiotides, and Asimakis Pappas
- Subjects
Colposcopy ,medicine.medical_specialty ,Pathology ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Population ,Obstetrics and Gynecology ,General Medicine ,medicine.disease ,NASBA ,Gastroenterology ,Exact test ,Squamous intraepithelial lesion ,Internal medicine ,parasitic diseases ,Medicine ,Outpatient clinic ,business ,Prospective cohort study ,education ,Ascus - Abstract
Objective To examine the diagnostic accuracy of nucleic acid sequence based amplification (NASBA) and flow cytometry for E6&7 human papillomavirus (HPV) mRNA detection in the triage of minor cytological abnormalities. Design Prospective diagnostic accuracy study. Setting Gynecology outpatient clinics of a university hospital. Population 472 women with low-grade squamous intraepithelial lesion (LSIL) or atypical squamous cells of undetermined significance (ASCUS). Methods Residual material of the liquid-based smears was tested by NASBA and by flow cytometry for E6E Fisher's exact test). In the LSIL group the NASBA accuracy indices for CIN3+ were: sensitivity 75%, specificity 78.7% and positivity rate 20.8%, and for flow 77.8%, 64.5% and 35.9%, respectively. Conclusions NASBA has favorable specificity and positivity rates for triaging LSIL prior to colposcopy. A relatively low sensitivity warrants cytological surveillance of the NASBA-negative LSILs. Flow cytometry does not perform as well overall.
- Published
- 2012