1. Adipose tissue-derived extracellular matrix hydrogels as a release platform for secreted paracrine factors
- Author
-
Berend van der Lei, Joris A van Dongen, Prashant K. Sharma, Hieronymus P Stevens, Vasilena E Getova, Gabriel Romero Liguori, Linda A. Brouwer, Martin C. Harmsen, Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT), and Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
- Subjects
acellular matrix ,Medicine (miscellaneous) ,Adipose tissue ,wound healing ,02 engineering and technology ,Extracellular matrix ,Research Articles ,Glycosaminoglycans ,0303 health sciences ,Decellularization ,Sulfates ,Viscosity ,Chemistry ,Hydrogels ,Stromal vascular fraction ,Extracellular Matrix ,Cell biology ,medicine.anatomical_structure ,DIFFERENTIATION ,Adipose Tissue ,Self-healing hydrogels ,decellularization ,STEM-CELLS ,Research Article ,DIABETIC FOOT ,0206 medical engineering ,Biomedical Engineering ,Neovascularization, Physiologic ,macromolecular substances ,STROMAL-VASCULAR FRACTION ,complex mixtures ,Biomaterials ,03 medical and health sciences ,Paracrine signalling ,AGE ,Paracrine Communication ,growth factors ,Diabetes Mellitus ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,Fibroblast ,Cell Proliferation ,030304 developmental biology ,mesenchymal stem cells ,Fibroblasts ,020601 biomedical engineering ,Elasticity ,COLLAGEN ,FAT ,Culture Media, Conditioned ,Cattle ,Stromal Cells ,hydrogel ,Wound healing ,GLYCATION - Abstract
INTRODUCTION: Fat grafting is an established clinical intervention to promote tissue repair. The role of the fat's extracellular matrix (ECM) in regeneration is largely neglected. We investigated in vitro the use of human adipose tissue-derived ECM hydrogels as release platform for factors secreted by adipose derived stromal cells (ASC) MATERIAL & METHODS: Lipoaspirates from non-diabetic and diabetic donors were decellularized. Finely powdered acellular ECM was evaluated for cell remainders and DNA content. Acellular ECM was digested and hydrogels were formed at 37 °C and their viscoelastic relaxation properties investigated. Release of ASC-released factors from hydrogels was immune-assessed and bio-activity was determined by fibroblast proliferation and migration and endothelial angiogenesis.RESULTS: Acellular ECM contained no detectable cell remainders and negligible DNA contents. Viscoelastic relaxation measurements yielded no data for diabetic-derived hydrogels due to gel instability. Hydrogels released several ASC-released factors concurrently in a sustained fashion. Functionally, released factors stimulated fibroblast proliferation and migration as well as angiogenesis. No difference between non-diabetic and diabetic hydrogels in release of factors was measured.CONCLUSION: Adipose ECM hydrogels incubated with released factors by ASC are a promising new therapeutic modality to promote several important wound healing related processes by releasing factors in a controlled way.
- Published
- 2019