1. Prognostic value of detection of CD99 + , CD45 − cells in peripheral blood by flow cytometry in children with Ewing sarcoma
- Author
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Wael Zekri, Ahmed Kamel, Iman Zaky, Asmaa Salama, Mohamed El-Shanshory, Nahla El-Sharkawy, Osama Zakzok, Shebl Said, and Ismail Elantably
- Subjects
Chemotherapy ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Hazard ratio ,CD99 ,Cancer ,Hematology ,medicine.disease ,Gastroenterology ,Confidence interval ,Metastasis ,Oncology ,Internal medicine ,Pediatrics, Perinatology and Child Health ,medicine ,Sarcoma ,Prospective cohort study ,business - Abstract
BACKGROUND Early detection of metastasis and recurrence of Ewing sarcoma (ES) is important for early management. This work aimed to detect CD99+ , CD45- cells in peripheral blood by flow cytometry (FC) before and during chemotherapy and evaluate their prognostic significance. PROCEDURE This prospective cohort study was carried out on 60 children newly diagnosed with ES at Children Cancer Hospital-Egypt 57357 and 40 healthy children control group. Detection of CD99+ , CD45- cells in peripheral blood was accomplished by FC at baseline before treatment and after five cycles of chemotherapy. Samples were classified as positive if they had more than the upper limit of cells observed in the control cases. Correlation between FC results and relapse and overall survival (OS) after one year was performed. RESULTS Median percentage of CD99+ , CD45- cells was significantly increased in patients compared with controls (0.002% vs 0%, respectively, P
- Published
- 2021
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