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Your search keyword '"Otoi, Takeshige"' showing total 39 results
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39 results on '"Otoi, Takeshige"'

7. Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle‐yaks affects spermatogenic cell fate and sterility

Author

Shimazaki, Megumi, primary, Wittayarat, Manita, additional, Sambuu, Rentsenkhand, additional, Sugita, Asami, additional, Kawaguchi, Masaki, additional, Hirata, Maki, additional, Tanihara, Fuminori, additional, Takagi, Mitsuhiro, additional, Taniguchi, Masayasu, additional, Otoi, Takeshige, additional, and Sato, Yoko, additional

Published
2022
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11. Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle–yak showing infertility

Author

Phakdeedindan, Praopilas, primary, Wittayarat, Manita, additional, Tharasanit, Theerawat, additional, Techakumphu, Mongkol, additional, Shimazaki, Megumi, additional, Sambuu, Rentsenkhand, additional, Hirata, Maki, additional, Tanihara, Fuminori, additional, Taniguchi, Masayasu, additional, Otoi, Takeshige, additional, and Sato, Yoko, additional

Published
2021
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15. Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single‐stranded oligodeoxynucleotides

Author

Wittayarat, Manita, primary, Hirata, Maki, additional, Namula, Zhao, additional, Sato, Yoko, additional, Nguyen, Nhien T., additional, Le, Quynh A., additional, Lin, Qingyi, additional, Takebayashi, Koki, additional, Tanihara, Fuminori, additional, and Otoi, Takeshige, additional

Published
2021
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19. Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin ( MSTN ) genes on the development and gene editing of porcine zygotes

Author

Le, Quynh A., primary, Hirata, Maki, additional, Nguyen, Nhien T., additional, Takebayashi, Koki, additional, Wittayarat, Manita, additional, Sato, Yoko, additional, Namula, Zhao, additional, Nii, Masahiro, additional, Tanihara, Fuminori, additional, and Otoi, Takeshige, additional

Published
2020
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21. Relationship among ovarian follicular status, developmental competence of oocytes, and anti‐Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts

Author

Tanihara, Fuminori, primary, Hirata, Maki, additional, Iizuka, Satoru, additional, Sairiki, Shinya, additional, Nii, Masahiro, additional, Nguyen, Nhien Thi, additional, Le, Quynh Anh, additional, Hirano, Takayuki, additional, and Otoi, Takeshige, additional

Published
2019
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26. Effects of chlorogenic acid ( CGA ) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization

Author

Nguyen, Thanh‐Van, primary, Wittayarat, Manita, additional, Do, Lanh Thi Kim, additional, Nguyen, Thanh Van, additional, Nii, Masahiro, additional, Namula, Zhao, additional, Kunihara, Toshiki, additional, Tanihara, Fuminori, additional, Hirata, Maki, additional, and Otoi, Takeshige, additional

Published
2018
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32. Effects of long-termin vitroexposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium

Author

SAMBUU, Rentsenkhand, primary, TAKAGI, Mitsuhiro, additional, NAMULA, Zhao, additional, NII, Masahiro, additional, TANIGUCHI, Masayasu, additional, UNO, Seiich, additional, KOKUSHI, Emiko, additional, TSHERING, Chenga, additional, SANTOS, Regiane Rodrigues dos, additional, FINK-GREMMELS, Johanna, additional, and OTOI, Takeshige, additional

Published
2012
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34. Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.

Author

Tanihara F, Hirata M, Nguyen NT, Le QA, Hirano T, Takemoto T, Nakai M, Fuchimoto DI, and Otoi T

Subjects
Animals, Female, Fertilization in Vitro, RNA, Guide, CRISPR-Cas Systems genetics, Blastocyst, CRISPR-Associated Protein 9 genetics, CRISPR-Cas Systems genetics, Clustered Regularly Interspaced Short Palindromic Repeats genetics, Electroporation, Gene Editing methods, Homeodomain Proteins genetics, Mutation, Swine genetics, Trans-Activators genetics, Zygote
Abstract

Recently, we established the GEEP ("gene editing by electroporation of Cas9 protein") method, in which the CRISPR/Cas9 system, consisting of a Cas9 protein and single guide RNA (sgRNA), is introduced into pig zygotes by electroporation and thus induces highly efficient targeted gene disruption. In this study, we examined the effects of sgRNA on the blastocyst formation of porcine embryos and evaluated their genome-editing efficiency. To produce an animal model for diabetes, we targeted PDX-1 (pancreas duodenum homeobox 1), a gene that is crucial for pancreas development during the fetal period and whose monoallelic disruption impairs insulin secretion. First, Cas9 protein with different sgRNAs that targeted distinct sites in the PDX-1 exon 1 was introduced into in vitro-fertilized zygotes by the GEEP method. Of the six sgRNAs tested, three sgRNAs (sgRNA1, 2, and 3) successfully modified PDX-1 gene. The blastocyst formation rate of zygotes edited with sgRNA3 was significantly (p < 0.05) lower than that of control zygotes without the electroporation treatment. Our study indicates that the GEEP method can be successfully used to generate PDX-1 mutant blastocysts, but the development and the efficiency of editing the genome of zygotes may be affected by the sgRNA used for CRISPR/Cas9 system., (© 2018 Japanese Society of Animal Science.)

Published
2019
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35. Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.

Author

Wittayarat M, Sato Y, Do LT, Chatdarong K, Tharasanit T, Techakumphu M, Taniguchi M, and Otoi T

Subjects
Acetylation, Animals, Cats, Cattle, Cellular Reprogramming Techniques, Female, Fertilization in Vitro, Histones metabolism, Lysine, Methylation, Species Specificity, Embryonic Development, Epigenesis, Genetic drug effects, Hydroxamic Acids pharmacology, Nuclear Transfer Techniques
Abstract

This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di-methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)-treated interspecies somatic cell nuclear transfer (iSCNT) cat-cow (TSA-iSCNT) embryos, TSA-untreated iSCNT cat-cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA-iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA-iSCNT embryos and IVF embryos at most following stages (2 h post-fusion / post-insemination (PF/PI) to eight-cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA-iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA-iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA-iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis., (© 2016 Japanese Society of Animal Science.)

Published
2017
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36. In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.

Author

Isobe T, Ikebata Y, Do LT, Tanihara F, Taniguchi M, and Otoi T

Subjects
Animals, Blastocyst, Cattle, Culture Media pharmacology, Female, Pregnancy, Cell Survival drug effects, Embryo Culture Techniques methods, Embryo Transfer, Embryonic Development drug effects, Freezing, Oocytes, Pregnancy, Animal, Sericins pharmacology
Abstract

The optimization of single-embryo culture conditions is very important, particularly in the in vitro production of bovine embryos using the ovum pick-up (OPU) procedure. The purpose of this study was to examine the development of embryos derived from oocytes obtained by OPU that were cultured either individually or in groups in medium supplemented with or without sericin and to investigate the viability of the frozen-thawed embryos after a direct transfer. When two-cell-stage embryos were cultured either individually or in groups for 7 days in CR1aa medium supplemented with or without 0.5% sericin, the rates of development to blastocysts and freezable blastocysts were significantly lower for the embryos cultured individually without sericin than for the embryos cultured in groups with or without sericin. Moreover, the rate of development to freezable blastocysts of the embryos cultured individually with sericin was significantly higher than that of the embryos cultured without sericin. When the frozen-thawed embryos were transferred directly to recipients, the rates of pregnancy, abortion, stillbirth and normal calving in the recipients were similar among the groups, irrespective of the culture conditions and sericin supplementation. Our findings indicate that supplementation with sericin during embryo culture improves the quality of the embryos cultured individually but not the viability of the frozen-thawed embryos after transfer to recipients., (© 2014 Japanese Society of Animal Science.)

Published
2015
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37. Roles of the zona pellucida and functional exposure of the sperm-egg fusion factor 'IZUMO' during in vitro fertilization in pigs.

Author

Tanihara F, Nakai M, Men NT, Kato N, Kaneko H, Noguchi J, Otoi T, and Kikuchi K

Subjects
Acrosome Reaction drug effects, Animals, Female, In Vitro Techniques, Male, Swine, Fertilization drug effects, Fertilization in Vitro, Immunoglobulins pharmacology, Membrane Proteins pharmacology, Oocytes physiology, Sperm-Ovum Interactions drug effects, Spermatozoa physiology, Zona Pellucida physiology
Abstract

The zona pellucida (ZP) is considered to play important roles in the prevention of polyspermy in mammalian oocytes. However, in pigs we have shown that the presence of the ZP accelerates sperm penetration into the ooplasm during in vitro fertilization (IVF). In the present study, we investigated the effects of the ZP on sperm binding, acrosomal status, and functional exposure of IZUMO, a critical factor involved in sperm-egg fusion, during IVF in pigs. We evaluated the numbers and acrosomal statuses of sperm binding to the ZP and oolemma, and being present in the ZP and perivitelline space (PVS) using ZP-intact and ZP-free oocytes. More sperm bound to the ZP than to the oolemma. The average number of sperm present in the PVS was 0.44-0.51 per oocyte, and all sperm had lost their acrosomes. The proportion of sperm that were immunopositive for anti-IZUMO antibody was significantly higher after they were passing or had passed through the ZP. Furthermore, addition of anti-IZUMO antibody to the fertilization medium significantly inhibited the penetration of sperm into ZP-free oocytes. These results suggest that, in pigs, the ZP induces the acrosome reaction, which is associated with the functional exposure of IZUMO, resulting in completion of fertilization., (© 2014 Japanese Society of Animal Science.)

Published
2014
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38. Japanese Black breeding herd exhibiting low blood urea nitrogen: a metabolic profile study examining the effect on reproductive performance.

Author

Watanabe U, Okamoto K, Miyamoto A, Otoi T, Yamato O, Tshering C, and Takagi M

Subjects
Animals, Cholesterol blood, Dietary Supplements, Fabaceae, Female, Postpartum Period metabolism, Protein Deficiency metabolism, Serum Albumin metabolism, Animal Feed, Animal Nutritional Physiological Phenomena physiology, Blood Urea Nitrogen, Cattle metabolism, Cattle physiology, Protein Deficiency physiopathology, Reproduction physiology
Abstract

Ten reared cows of a Japanese Black cattle herd in Kagoshima prefecture, Japan, exhibited extremely low blood urea nitrogen (BUN) concentration (2.6 ± 0.6 mg/dL). Examination of dietary feed nutrition and relevant pastureland soil content suggested a correlation with crude protein (CP) deficiency or unbalanced nutritional dietary feeds. Thirteen months after the introduction of a dietary remedial measure (bean cake supplementation), BUN, total cholesterol and albumin concentration from five of the original 10 cows increased significantly compared with their values of before the dietary remedy. The postpartum day open period was significantly lower after the dietary remedial measure than that before it. The abnormally low BUN levels of the cattle herd may be due to inadequate dietary nutritional content, primarily from the imbalance of total digestible nutrient and CP of the feed and far lower han average CP value. In conclusion, routine examination of serum biochemical parameters in Japanese Black breeding cattle may be a useful strategy for determining subclinical metabolic failure of cattle herds, and consequently, its effect on reproductive performance of the herd., (© 2012 Japanese Society of Animal Science.)

Published
2013
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39. Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium.

Author

Sambuu R, Takagi M, Namula Z, Nii M, Taniguchi M, Uno S, Kokushi E, Tshering C, dos Santos RR, Fink-Gremmels J, and Otoi T

Subjects
Animals, Cells, Cultured, Dose-Response Relationship, Drug, Fertilization drug effects, Male, Sperm Motility drug effects, Temperature, Time Factors, Zeranol adverse effects, Culture Media adverse effects, Estrogens, Non-Steroidal adverse effects, Fertilization in Vitro, Spermatozoa drug effects, Spermatozoa physiology, Swine physiology, Zearalenone adverse effects, Zeranol analogs & derivatives
Abstract

The effects of in vitro exposure of porcine spermatozoa to zearalenone (ZEN) and α-zearalenol (α-ZOL) were studied by evaluating several parameters of an in vitro fertilization (IVF) system. For this purpose, boar spermatozoa cultured with semen storage medium containing 0 (control), 10 and 1000 µg/L of ZEN and α-ZOL for 1 week at 5°C were used for IVF of in vitro matured oocytes. Overall, there were no significant differences in the rates of total penetration, monospermic fertilization, and polyspermic fertilization of oocytes inseminated with spermatozoa from the different groups. Similarly, ZEN and α-ZOL at 10 and 1000 µg/L did not have detrimental effects on the cleavage and development to blastocysts of oocytes after in vitro fertilization. Although the motility, viability, and plasma membrane integrity of spermatozoa significantly decreased after 3 weeks of storage compared to non-stored spermatozoa (P < 0.05), ZEN and α-ZOL at the evaluated concentrations did not exert detrimental effects on the above parameters, even after 3 weeks of storage. These results indicate that prolonged exposure of boar spermatozoa to ZEN and α-ZOL up to 1000 µg/L under reduced metabolic conditions does not affect their in vitro function., (© 2012 Japanese Society of Animal Science.)

Published
2013
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