37 results on '"Pilar Martínez"'
Search Results
2. Clinical outcomes after CPX‐351 in patients with high‐risk acute myeloid leukemia: A comparison with a matched cohort from the Spanish PETHEMA registry
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Teresa Bernal, Ainhoa Fernández Moreno, Almudena de LaIglesia, Celina Benavente, Ana García‐Noblejas, Daniel García Belmonte, Rosalía Riaza, Olga Salamero, Maria Angeles Foncillas, Alicia Roldán, Víctor Noriega Concepción, Laura Llorente González, Juan Miguel Bergua Burgués, Soraya Lorente de Uña, Gabriela Rodríguez‐Macías, Adolfo de la Fuente Burguera, Maria José García Pérez, Jose Luis López‐Lorenzo, Pilar Martínez, Concepción Aláez, Marta Callejas, Carmen Martínez‐Chamorro, José Rifón Roca, Lourdes Amador Barciela, Armando V. Mena Durán, Karoll Gómez Correcha, Esperanza Lavilla Rubira, María Luz Amigo, Ferran Vall‐llovera, Ana Garrido, María García‐Fortes, Dunia de Miguel Llorente, Anastasia Aules Leonardo, Carlos Cervero, Rosa Coll Jordá, Manuel M. Pérez‐Encinas, Marta Polo Zarzuela, Angela Figuera, Guillermo Rad, David Martínez‐Cuadrón, and Pau Montesinos
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acute myeloid leukemia ,clinical observations ,intensive chemotherapy ,real‐world ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background CPX‐351 is approved for the treatment of therapy related acute myeloid leukemia (t‐AML) and AML with myelodysplastic related changes (MRC‐AML). The benefits of this treatment over standard chemotherapy has not been addressed in well matched cohorts of real‐life patients. Methods Retrospective analysis of AML patients treated with CPX‐351 as per routine practice. A propensity score matching (PSM) was used to compare their main outcomes with those observed in a matched cohort among 765 historical patients receiving intensive chemotherapy (IC), all of them reported to the PETHEMA epidemiologic registry. Results Median age of 79 patients treated with CPX‐351 was 67 years old (interquartile range 62–71), 53 were MRC‐AML. The complete remission (CR) rate or CR without recovery (CRi) after 1 or 2 cycles of CPX‐351 was 52%, 60‐days mortality 18%, measurable residual disease
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- 2023
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3. A pediatric regimen for adolescents and young adults with Philadelphia chromosome‐negative acute lymphoblastic leukemia: Results of the ALLRE08 PETHEMA trial
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Josep‐Maria Ribera, Mireia Morgades, Pau Montesinos, Mar Tormo, Daniel Martínez‐Carballeira, José González‐Campos, Cristina Gil, Pere Barba, Raimundo García‐Boyero, Rosa Coll, María Pedreño, Jordi Ribera, Santiago Mercadal, Susana Vives, Andrés Novo, Eulàlia Genescà, Jesús‐María Hernández‐Rivas, Juan Bergua, María‐Luz Amigo, Ferran Vall‐Llovera, Pilar Martínez‐Sánchez, María Calbacho, Irene García‐Cadenas, Antoni Garcia‐Guiñon, María‐José Sánchez‐Sánchez, Marta Cervera, Evarist Feliu, Alberto Orfao, and the PETHEMA Group, Spanish Society of Hematology
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acute lymphoblastic leukemia ,adolescents and young adults ,pediatric treatment ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Pediatric‐based or ‐inspired trials have improved the prognosis of adolescents and young adults (AYA) with Philadelphia chromosome‐negative (Ph‐neg) acute lymphoblastic leukemia (ALL). Methods This study reports the results of treatment of the ALLRE08 trial, a full pediatric trial for AYA aged 15‐30 years with standard‐risk (SR) ALL. Results From 2008 to 2018, 89 patients (38 adolescents [15‐18 years] and 51 young adults [YA, 19‐30 years], median age: 20 [15‐29] years) were enrolled in the ALLRE08 trial. The complete response (CR) was 95%. Twenty‐two patients were transferred to a high‐risk (HR) protocol because of poor marrow response on day 14 (n = 20) or high‐level of end‐induction minimal residual response (MRD ≥ 0.25%, n = 2). Cumulative incidence of relapse (CIR) at 5 years was 35% (95%CI: 23%‐47%), with significant differences between adolescents and YA: 13% (4%‐28%) vs 52% (34%‐67%), P = .012. No treatment‐related mortality was observed in 66/66 patients following the ALLRE08 trial vs 3/23 patients moved to a HR trial. The estimated 5‐year overall survival (OS) was 74% (95%CI: 63%‐85%), with significantly higher rates for adolescents vs YA: 87% (95%CI: 74%‐100%) vs 63% (46%‐80%), P = .021. Although CIR or OS were lower in patients who were transferred to a HR trial, the differences were not statistically significant (CIR: 34% [21%‐47%] vs 37% [14%‐61%]; OS: 78% [66%‐90%] vs 61% [31%;91%]). Conclusion A full pediatric trial is feasible and effective for AYA with Ph‐neg, SR‐ALL, with better results for adolescents than for YA. Outcome of patients with poor early response rescued with a HR trial was not significantly inferior.
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- 2020
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4. Real-Life Diagnostic Accuracy of MRI in Prenatal Diagnosis
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Manuel Recio Rodríguez, Cristina Andreu-Vázquez, Israel J. Thuissard-Vasallo, Raquel Cano Alonso, Carmina Bermejo López, Ines Tamarit Degenhardt, and Pilar Martínez Ten
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Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
There is some controversy about the value of fetal MRI in prenatal diagnosis, and most of the studies examine its accuracy in central nervous system (CNS) pathology. The objective of this retrospective study was to assess the diagnostic accuracy and usefulness of fetal MRI in the prenatal diagnosis of central nervous system (CNS) pathology and non-CNS pathology. Patients referred to the Radiology Department between 2007 and 2018 for a fetal MRI after detection of an anomaly in the fetal ultrasound, a high-risk pregnancy, or an inconclusive fetal ultrasound (n = 623) were included in the study. Postnatal diagnosis was used to assess the diagnostic accuracy of MRI. Fetal MRI was considered to provide additional information over fetal ultrasound when findings of the fetal MRI were not detected in the fetal ultrasound or when established a pathological condition that was not detected in the fetal ultrasound. Fetal MRI provided useful information for the perinatal management and prognosis over fetal ultrasound when findings of the fetal MRI changed the postnatal prognosis, leaded to the decision to legally terminate the pregnancy, changed prenatal or postnatal follow-up, or helped in the planning of prenatal or postnatal treatment. Fetal MRI offered an accurate diagnosis in 97% of cases (compared to 90.4% of fetal ultrasound; p
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- 2020
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5. Clinical outcomes after <scp>CPX</scp> ‐351 in patients with high‐risk acute myeloid leukemia: A comparison with a matched cohort from the Spanish <scp>PETHEMA</scp> registry
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Teresa Bernal, Ainhoa Fernández Moreno, Almudena de LaIglesia, Celina Benavente, Ana García‐Noblejas, Daniel García Belmonte, Rosalía Riaza, Olga Salamero, Maria Angeles Foncillas, Alicia Roldán, Víctor Noriega Concepción, Laura Llorente González, Juan Miguel Bergua Burgués, Soraya Lorente de Uña, Gabriela Rodríguez‐Macías, Adolfo de la Fuente Burguera, Maria José García Pérez, Jose Luis López‐Lorenzo, Pilar Martínez, Concepción Aláez, Marta Callejas, Carmen Martínez‐Chamorro, José Rifón Roca, Lourdes Amador Barciela, Armando V. Mena Durán, Karoll Gómez Correcha, Esperanza Lavilla Rubira, María Luz Amigo, Ferran Vall‐llovera, Ana Garrido, María García‐Fortes, Dunia de Miguel Llorente, Anastasia Aules Leonardo, Carlos Cervero, Rosa Coll Jordá, Manuel M. Pérez‐Encinas, Marta Polo Zarzuela, Angela Figuera, Guillermo Rad, David Martínez‐Cuadrón, and Pau Montesinos
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Tratamiento médico ,Cancer Research ,Oncology ,Leucemia mieloide ,Investigación ,Radiology, Nuclear Medicine and imaging ,Quimioterapia ,Cáncer - Abstract
Background CPX-351 is approved for the treatment of therapy related acute myeloid leukemia (t-AML) and AML with myelodysplastic related changes (MRC-AML). The benefits of this treatment over standard chemotherapy has not been addressed in well matched cohorts of real-life patients. Methods Retrospective analysis of AML patients treated with CPX-351 as per routine practice. A propensity score matching (PSM) was used to compare their main outcomes with those observed in a matched cohort among 765 historical patients receiving intensive chemotherapy (IC), all of them reported to the PETHEMA epidemiologic registry. Results Median age of 79 patients treated with CPX-351 was 67 years old (interquartile range 62–71), 53 were MRC-AML. The complete remission (CR) rate or CR without recovery (CRi) after 1 or 2 cycles of CPX-351 was 52%, 60-days mortality 18%, measurable residual disease
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- 2023
6. A “Mute Wooing”
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Benedí and, Pilar Martínez, primary and Savarese, Ralph James, additional
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- 2022
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7. Improving the prediction of acute myeloid leukaemia outcomes by complementing mutational profiling withex vivochemosensitivity
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Joaquin Martinez-Lopez, Rosa Ayala, Julian Gorrochategui, Jaime Pérez-Oteyza, Inmaculada Rapado, Joan Ballesteros, Eva Barragán, Pau Montesinos, José Luis Rojas, David Martínez-Cuadrón, Esther Onecha, María Linares, Elena Magro, Blanca Boluda, Pilar Martínez-Sánchez, Claudia Sargas, Yanira Ruiz-Heredia, Jesús Villoria, and Pilar Herrera
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Adult ,Male ,Oncology ,Drug ,medicine.medical_specialty ,Adolescent ,media_common.quotation_subject ,Drug resistance ,Cohort Studies ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Aged ,media_common ,Aged, 80 and over ,biology ,business.industry ,Hazard ratio ,EZH2 ,Hematology ,Middle Aged ,Leukemia, Myeloid, Acute ,Treatment Outcome ,KMT2A ,030220 oncology & carcinogenesis ,Mutation ,Cohort ,biology.protein ,Female ,Myeloid leukaemia ,business ,Ex vivo ,030215 immunology - Abstract
Refractoriness to induction therapy and relapse after complete remission are the leading causes of death in patients with acute myeloid leukaemia (AML). This study focussed on the prediction of response to standard induction therapy and outcome of patients with AML using a combined strategy of mutational profiling by next-generation sequencing (NGS, n = 190) and ex vivo PharmaFlow testing (n = 74) for the 10 most widely used drugs for AML induction therapy, in a cohort of adult patients uniformly treated according to Spanish PETHEMA guidelines. We identified an adverse mutational profile (EZH2, KMT2A, U2AF1 and/or TP53 mutations) that carries a greater risk of death [hazard ratio (HR): 3·29, P < 0·0001]. A high correlation was found between the ex vivo PharmaFlow results and clinical induction response (69%). Clinical correlation analysis showed that the pattern of multiresistance revealed by ex vivo PharmaFlow identified patients with a high risk of death (HR: 2·58). Patients with mutation status also ran a high risk (HR 4·19), and the risk was increased further in patients with both adverse profiles (HR 4·82). We have developed a new score based on NGS and ex vivo drug testing for AML patients that improves upon current prognostic risk stratification and allows clinicians to tailor treatments to minimise drug resistance.
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- 2020
8. Clinical and demographic characteristics of fatal anaphylaxis in Spain (1998‐2011): A comparison between a series from the hospital system and a national forensic series
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Esperanza Navarro-Escayola, Nuria Alberti-Masgrau, Miguel A. Tejedor-Alonso, Mar Moro-Moro, Pilar Martínez-Fernández, and Gloria Vallejo-de-Torres
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Adult ,Male ,0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,Databases, Factual ,Immunology ,Drug allergy ,Logistic regression ,03 medical and health sciences ,0302 clinical medicine ,Food allergy ,medicine ,Humans ,Immunology and Allergy ,Clinical significance ,Anaphylaxis ,Cause of death ,business.industry ,Incidence (epidemiology) ,Forensic Medicine ,medicine.disease ,Comorbidity ,030104 developmental biology ,030228 respiratory system ,Spain ,Female ,business - Abstract
Background Reports of fatal anaphylaxis remain scarce because of the rarity of the condition and the fact that information is limited to a few countries. Objective Our objective was to investigate clinical and demographic characteristics and the causes of fatal anaphylaxis in Spain using two databases of cases of fatal anaphylaxis. Methods We analysed fatal anaphylaxis in a series from the Spanish hospital system and a series from the National Institute of Toxicology and Forensic Sciences (Instituto Nacional de Toxicologia y Ciencias Forenses [INTCF]), which predominantly comprise extrahospital deaths. Deaths from the Spanish hospital system were retrieved from among all deaths occurring during 1998-2011 using codes related to anaphylaxis. Deaths due to anaphylaxis in the INTCF database during the same period were retrieved by 2 allergists, who identified cases in which anaphylaxis was a possible cause of death. A logistic regression model was constructed to predict the characteristics of fatal anaphylaxis in each database. Results The incidence of death by anaphylaxis in Spain using both databases was 0.25 (95% CI, 0.24-0.26) deaths per million person-years. The most frequent causes of death in the hospital system were drugs (46.1%), unknown causes (40.0%), and foods (10.4%); in the INTCF, the most common causes of death were drugs (47.2%), insect stings (30.6%), and foods (11.1%). The logistic regression model showed that fatal anaphylaxis due to unknown causes (OR 15.2, 95% CI 1.8-129.8) was more likely in the hospital database, whereas insect stings (OR 100, 95% CI 10-833.3) and previous atopic comorbidity (OR 15.2, 95% CI 6.3-33.3) were more likely in the INTCF database. Conclusions & clinical relevance The estimated frequency of fatal anaphylaxis in Spain was among the lowest reported. Future studies of fatal anaphylaxis should use databases from different origins in order to show the considerable heterogeneity in this type of death.
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- 2018
9. Endothelial KCa1.1 and KCa3.1 channels mediate rat intrarenal artery endothelium‐derived hyperpolarization response
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Dolores Prieto, Estéfano Pinilla, Luis Rivera, Albino García-Sacristán, Ana Sánchez, María Pilar Martínez, Ralf Köhler, and Mercedes Muñoz
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0301 basic medicine ,Kidney ,Renal circulation ,Endothelium ,Electrical impedance myography ,EDH ,Physiology ,Chemistry ,030204 cardiovascular system & hematology ,Hyperpolarization (biology) ,Interlobar arteries ,KCa channels ,Renal Circulation ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,medicine ,Artery Endothelium ,Acetylcholine ,medicine.drug - Abstract
Aim: Endothelium-derived hyperpolarization (EDH)-mediated response plays an essential role in the control of kidney preglomerular circulation, but the identity of the K+ channels involved in this response is still controversial. We hypothesized that large- (KCa 1.1), intermediate- (KCa 3.1) and small (KCa 2.3) -conductance Ca2+ -activated K+ (KCa) channels are expressed in the endothelium of the preglomerular circulation and participate in the EDH-mediated response.Methods: We study the functional expression of different K+ channels in non-cultured, freshly isolated native endothelial cells (ECs) of rat intrarenal arteries using immunofluorescence and the patch-clamp technique. We correlate this with vasorelaxant responses ex vivo using wire myography.Results: Immunofluorescence revealed the expression of KCa 1.1, KCa 3.1 and KCa 2.3 channels in ECs. Under voltage-clamp conditions, acetylcholine induced a marked increase in the outward currents in these cells, sensitive to the blockade of KCa 1.1, KCa 3.1 and KCa 2.3 channels, respectively. Isometric myography experiments, under conditions of endothelial nitric oxide synthase and cyclooxygenase inhibition, showed that blockade either of KCa 1.1 or KCa 3.1 channels was able to reduce the endothelium-derived vasorelaxation of isolated interlobar arteries, while their combined blockade completely abolished it. In contrast, blockade of KCa 2.3 channels did not reduce this vasorelaxant response, despite being functionally expressed in the endothelial cells.Conclusion: This study shows that KCa 1.1 and KCa 3.1 channels are functionally expressed at the renal vascular endothelium and play a central role in the EDH-mediated relaxation of kidney preglomerular arteries, which is important in the control of renal blood flow and glomerular filtration rate.
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- 2020
10. Autologous stem cell transplantation may be curative for patients with follicular lymphoma with early therapy failure who reach complete response after rescue treatment
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Ana Jiménez-Ubieto, Laura Magnano, Pilar Martínez-Sánchez, Elena Pérez, Carmen Marrero, Carmen Albo, Javier Lopez Jimenez, Santiago Mercadal, María Manzanares, Isidro Jarque, Reyes Arranz, Lucrecia Yáñez, Alejandro Martín, Juan José Lahuerta, Carlos Vallejo, Carlos Grande, Antonio Salar, José Javier Ferreiro, Miguel T. Hernández-García, Dolores Caballero, Luis Palomera, José M. Moraleda, Armando López-Guillermo, Sabela Bobillo, Andrea Galeo, Erika Coria, and Silvana Novelli
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Adult ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Follicular lymphoma ,Early Relapse ,Early Therapy ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Autologous stem-cell transplantation ,Internal medicine ,medicine ,Humans ,Registries ,Autografts ,Lymphoma, Follicular ,Complete response ,Aged ,business.industry ,Hematopoietic Stem Cell Transplantation ,Hematology ,General Medicine ,Middle Aged ,medicine.disease ,Rescue treatment ,Survival Rate ,Spain ,030220 oncology & carcinogenesis ,Female ,Rituximab ,business ,030215 immunology - Published
- 2018
11. Field evaluation of biocontrol agents against black‐foot and Petri diseases of grapevine
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Pilar Martínez‐Diz, María, primary, Díaz‐Losada, Emilia, additional, Andrés‐Sodupe, Marcos, additional, Bujanda, Rebeca, additional, Maldonado‐González, María M, additional, Ojeda, Sonia, additional, Yacoub, Amira, additional, Rey, Patrice, additional, and Gramaje, David, additional
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- 2020
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12. Augmented oxidative stress and preserved vasoconstriction induced by hydrogen peroxide in coronary arteries in obesity: role of COX-2
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Albino García-Sacristán, Belén Climent, Mercedes Salaices, Elvira Santiago, Luis Rivera, María Pilar Martínez, Ana M. Briones, Mercedes Muñoz, and Dolores Prieto
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0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Vascular smooth muscle ,Endothelium ,business.industry ,Vasodilation ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Coronary arteries ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,medicine ,medicine.symptom ,Prostaglandin E2 ,Receptor ,business ,Oxidative stress ,Vasoconstriction ,medicine.drug - Abstract
Background and Purpose Oxidative stress plays a key role in the vascular and metabolic abnormalities associated with obesity. Herein we assessed whether obesity can increase coronary vasoconstriction induced by hydrogen peroxide (H2O2) and the signalling pathways involving cyclooxygenase 2 (COX-2) and superoxide (O2.-) generation. Experimental Approach Contractile responses to H2O2 and O2.- generation were measured in coronary arteries from genetically obese Zucker rats (OZR) compared to lean Zucker rats (LZR). Key Results Both basal and H2O2-stimulated O2.- production were enhanced in coronary arteries from OZR, but H2O2-induced vasoconstriction was unchanged. The selective COX-2 inhibitor NS398 significantly reduced H2O2-induced contractions in endothelium-denuded arteries from LZR and OZR, but only in endothelium-intact arteries from LZR. PGI2 receptor (IP) antagonism modestly reduced the vasoconstrictor action of H2O2 while antagonism of the prostaglandin E2 (PGE2) receptor 4 (EP4) enhanced H2O2 contractions in arteries from OZR but not LZR. Basal release of COX-2-derived PGE2 was higher in coronary arteries from OZR where the selective agonist of EP4 receptors TCS 2519 evoked potent relaxations. COX-2 was up-regulated after acute exposure to H2O2 in coronary endothelium and vascular smooth muscle (VSM) and inhibition of COX-2 markedly reduced H2O2-elicited O2.- generation in coronary arteries and myocardium. Expression of Nox subunits in VSM and NADPH-stimulated O2.- generation were enhanced and contributed to H2O2 vasoconstriction in arteries from obese rats. Conclusion and Implications COX-2 contributes to cardiac oxidative stress and to the endothelium-independent O2.-mediated coronary vasoconstriction induced by H2O2 in obesity, which is offset by the release of COX-2-derived endothelial PGE2 acting on EP4 vasodilator receptors. This article is protected by copyright. All rights reserved.
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- 2016
13. Dietary treatment modulates mast cell phenotype, density, and activity in adult eosinophilic oesophagitis
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Teresa Mota-Huertas, Jesús González-Cervera, Ángel Arias, Marina Fortea, Alfredo J. Lucendo, María Vicario, Jose Luis Yagüe-Compadre, Ana M González-Castro, and Pilar Martínez-Fernández
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Adult ,Male ,CPA3 ,Adolescent ,Biopsy ,Immunology ,Gene Expression ,Tryptase ,Immunophenotyping ,Leukocyte Count ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Immunology and Allergy ,Mast Cells ,Eosinophilic esophagitis ,CCL11 ,Mucous Membrane ,biology ,Chymase ,Eosinophilic Esophagitis ,Middle Aged ,Eosinophil ,medicine.disease ,Eosinophils ,Chemotaxis, Leukocyte ,Phenotype ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,Female ,030211 gastroenterology & hepatology ,Esophagoscopy ,CCL26 ,CCL24 ,Biomarkers ,Follow-Up Studies - Abstract
BACKGROUND Mast cells (MCs) are abundant in the inflammatory infiltrate in eosinophilic oesophagitis (EoE), but decrease with disease remission. However, their phenotype, role in the pathophysiology of the disease, and modulation after effective dietary therapy are still unclear. OBJECTIVE To define the phenotype of oesophageal MCs, their modulation through dietary therapy, and their association with clinical manifestations of EoE. METHODS Oesophageal mucosal samples from 10 adult patients with EoE obtained before and after effective six-food elimination diet (SFED) therapy, as well as from 10 control subjects were analysed. Eosinophil and MC density were quantified. Gene expression of chemoattractants for eosinophils (CCL11, CCL24, and CCL26), MCs (SCF), and their receptors (CCR3 and SCFR, respectively) were assessed by means of qPCR. Gene and protein expression of specific MC proteases (CPA3, CMA, and TPSB2) were evaluated with qPCR and immunofluorescence. Clinical manifestations and atopic background were recorded. RESULTS MC density was significantly increased in EoE compared with controls, decreasing after dietary treatment (18.6 to 1.44 cells/hpf, respectively; P < 0.001). The MCTC subtype predominated in the oesophageal mucosa (90%) in both patients with EoE and controls. Gene expression of MC-related proteases, eotaxins, and SCF were up-regulated in patients with EoE, but significantly decreased after therapy, regardless of atopic background. Epithelial peaks of MCs and eosinophils were significantly associated (ρ = 0.80) in EoE and correlated with the symptom score (ρ = 0.78). Gene expression of MC proteases and eotaxins also correlated with the symptom score (P < 0.05). CONCLUSIONS AND CLINICAL RELEVANCE MC and its proteases seem to play a relevant role in the pathophysiology and symptoms of EoE, which can be reversed after effective dietary treatment.
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- 2015
14. Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from insulin-resistant obese rats: role of ETAand ETBreceptors
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Albino García-Sacristán, Dolores Prieto, Medardo Hernández, Pilar Martínez, Alvaro Sanchez, Mercedes Muñoz, and Sara Benedito
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Pharmacology ,medicine.medical_specialty ,Endothelium ,Endothelin B Receptor Antagonists ,Erectile tissue ,medicine.disease ,Endothelin 1 ,Endothelin A Receptor Antagonists ,Nitric oxide ,chemistry.chemical_compound ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,medicine ,medicine.symptom ,Endothelial dysfunction ,Vasoconstriction - Abstract
Conclusions and Implications ET-1 stimulates ETA-mediated NADPH oxidase-dependent ROS generation, which inhibits endothelial NO bioavailability and contributes to ET-1-induced contraction in healthy penile arteries. Enhanced vascular expression of ETB receptors contributes to augmented ROS production, endothelial dysfunction and increased vasoconstriction in erectile tissue from insulin-resistant obese rats. Hence, antagonism of ETB receptors might improve the ED associated with insulin-resistant states.
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- 2014
15. Pre- and post-junctional bradykinin B2receptors regulate smooth muscle tension to the pig intravesical ureter
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Vítor S. Fernandes, Paz Recio, Albino García-Sacristán, Luis M. Orensanz, Ana Cristina Martínez, Medardo Hernández, María Victoria Barahona, María Elvira López-Oliva, Salvador Bustamante, Ana S. F. Ribeiro, Dolores Prieto, Igor Blaha, and María Pilar Martínez
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Agonist ,medicine.medical_specialty ,medicine.drug_class ,Kallidin ,business.industry ,Urology ,Smooth muscle layer ,030232 urology & nephrology ,Bradykinin ,Smooth muscle contraction ,Neurotransmission ,Contractility ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,chemistry ,Internal medicine ,medicine ,030212 general & internal medicine ,Neurology (clinical) ,business ,Receptor - Abstract
Aims Neuronal and non-neuronal bradykinin (BK) receptors regulate the contractility of the bladder urine outflow region. The current study investigates the role of BK receptors in the regulation of the smooth muscle contractility of the pig intravesical ureter. Methods Western blot and immunohistochemistry were used to show the expression of BK B1 and B2 receptors and myographs for isometric force recordings. Results B2 receptor expression was consistently detected in the intravesical ureter urothelium and smooth muscle layer, B1 expression was not detected where a strong B2 immunoreactivity was observed within nerve fibers among smooth muscle bundles. On ureteral strips basal tone, BK induced concentration-dependent contractions, were potently reduced by extracellular Ca2+ removal and by B2 receptor and voltage-gated Ca2+ (VOC) channel blockade. BK contraction did not change as a consequence of urothelium mechanical removal or cyclooxygenase and Rho-associated protein kinase inhibition. On 9,11-dideoxy-9a,11a-methanoepoxy prostaglandin F2α (U46619)-precontracted samples, under non-adrenergic non-cholinergic (NANC) and nitric oxide (NO)-independent NANC conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. Kallidin, a B1 receptor agonist, failed to increase preparation basal tension or to induce relaxation on U46619-induced tone. Conclusions The present results suggest that BK produces contraction of pig intravesical ureter via smooth muscle B2 receptors coupled to extracellular Ca2+ entry mainly via VOC (L-type) channels. Facilitatory neuronal B2 receptors modulating NO-dependent or independent NANC inhibitory neurotransmission are also demonstrated.
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- 2014
16. Plasma ceramides relate to mild cognitive impairment in middle‐aged men: The Maastricht Study
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Daan vanKruining, Mario Losen, Simone M. Crivelli, Joost J. A. deJong, Jacobus F. A. Jansen, Walter H. Backes, Jennifer Monereo‐Sánchez, Martin P. J. vanBoxtel, Sebastian Köhler, David E. J. Linden, Miranda T. Schram, Michelle M. Mielke, and Pilar Martinez‐Martinez
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Alzheimer's disease ,biomarkers ,ceramide ,ceramide risk score ,ceramide transfer protein ,magnetic resonance imaging ,Neurology. Diseases of the nervous system ,RC346-429 ,Geriatrics ,RC952-954.6 - Abstract
Abstract Introduction There is an urgent need for biomarkers identifying individuals at risk of early‐stage cognitive impairment. Using cross‐sectional data from The Maastricht Study, this study included 197 individuals with mild cognitive impairment (MCI) and 200 cognitively unimpaired individuals aged 40 to 75, matched by age, sex, and educational level. Methods We assessed the association of plasma sphingolipid and ceramide transfer protein (CERT) levels with MCI and adjusted for potentially confounding risk factors. Furthermore, the relationship of plasma sphingolipids and CERTs with magnetic resonance imaging brain volumes was assessed and age‐ and sex‐stratified analyses were performed. Results Associations of plasma ceramide species C18:0 and C24:1 and combined plasma ceramide chain lengths (ceramide risk score) with MCI were moderated by sex, but not by age, and higher levels were associated with MCI in men. No associations were found among women. In addition, higher levels of ceramide C20:0, C22:0, and C24:1, but not the ceramide risk score, were associated with larger volume of the hippocampus after controlling for covariates, independent of MCI. Although higher plasma ceramide C18:0 was related to higher plasma CERT levels, no association of CERT levels was found with MCI or brain volumes. Discussion Our results warrant further analysis of plasma ceramides as potential markers for MCI in middle‐aged men. In contrast to previous studies, no associations of plasma sphingolipids with MCI or brain volumes were found in women, independent of age. These results highlight the importance of accounting for sex‐ and age‐related factors when examining sphingolipid and CERT metabolism related to cognitive function.
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- 2023
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17. Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops
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Lina P. Rivera, Pedro F. Mateos, Ma de la Encarnación Velázquez, Marta Marcos-García, Pilar Martínez-Hidalgo, Raúl Rivas, Esther Menéndez, José David Flores-Félix, Paula García-Fraile, and Eustoquio Martínez-Molina
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biology ,Biofertilizer ,fungi ,food and beverages ,Soil Science ,Lactuca ,Plant Science ,biology.organism_classification ,medicine.disease_cause ,Endophyte ,Rhizobium leguminosarum ,Rhizobia ,Botany ,medicine ,Rhizobium ,Phaseolus ,Daucus carota - Abstract
Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant-growth-promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Phaseolus vulgaris nodules. This strain was able to produce siderophores and indole acetic acid and to solubilize phosphate. Confocal microscopy showed that this strain was able to colonize the roots of two horticultural crops, Lactuca sativa L. (lettuce) and Daucus carota L. (carrot). Strain PEPV16 was also able to promote the plant growth of both plant species increasing the dry matter of shoots and roots of lettuce and carrots, respectively, as well as to increase the uptake of N and P in the edible parts of both plant species. These data confirmed the suitability of Rhizobium as biofertilizer for nonlegumes.
- Published
- 2013
18. Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle
- Author
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Joaquín Carballido, Ana Martínez-Sáenz, María Pilar Martínez, Ana S. F. Ribeiro, Paz Recio, Luis M. Orensanz, Medardo Hernández, María Ruth Pazos, Vítor S. Fernandes, Dolores Prieto, Salvador Bustamante, and Albino García-Sacristán
- Subjects
Agonist ,medicine.medical_specialty ,business.industry ,Kallidin ,medicine.drug_class ,Urology ,Pig bladder ,Bradykinin ,Smooth muscle contraction ,Neurotransmission ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,Extracellular ,Medicine ,Neurology (clinical) ,business ,Receptor - Abstract
Aims The current study investigates the role played by bradykinin (BK) receptors in the contractility to the pig bladder neck smooth muscle. Methods Bladder neck strips were mounted in myographs for isometric force recordings and BK receptors expression was also determined by immunohistochemistry. Results B2 receptor expression was observed in the muscular layer and urothelium whereas B1 expression was consistent detected in urothelium. A strong B2 immunoreactivity was also observed within nerve fibers among smooth muscle bundles. On urothelium-denuded preparations basal tone, BK induced concentration-dependent contractions which were reduced in urothelium-intact samples, by extracellular Ca2+ removal and by blockade of B2 receptors and voltage-gated Ca2+ (VOC) and non-VOC channels, and increased by cyclooxygenase (COX) inhibition. On phenylephrine-precontracted denuded strips, under non-adrenergic non-cholinergic (NANC) conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. In urothelium-intact samples, the B1 receptor agonist kallidin promoted concentration-dependent relaxations which were reduced by blockade of B1 receptors, COX, COX-1 and large-conductance Ca2+-activated K+ (BKCa) channels and abolished in urothelium-denuded samples and in K+-enriched physiological saline solution-precontracted strips. Conclusions These results suggest that BK produces contraction of pig bladder neck via smooth muscle B2 receptors coupled to extracellular Ca2+ entry via VOC and non-VOC channels with a minor role for intracellular Ca2+ mobilization. Facilitatory neuronal B2 receptors modulating NANC inhibitory neurotransmission and urothelial B1 receptors producing relaxation via the COX-1 pathway and BKCa channel opening are also demonstrated. Neurourol. Urodynam. 33:558–565, 2014. © 2013 Wiley Periodicals, Inc.
- Published
- 2013
19. Clofarabine-based chemotherapy for relapsed/refractory adult acute lymphoblastic leukemia and lymphoblastic lymphoma. The Spanish experience
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María Calbacho, Pilar Martínez-Sánchez, Antonia Sampol, Javier Bueno, Raimundo García-Boyero, Josep-Maria Ribera, Josefina Serrano, Pere Barba, José González, and Pascual Fernández
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Adult ,Male ,Antimetabolites, Antineoplastic ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Salvage therapy ,Gastroenterology ,Young Adult ,Refractory ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Clofarabine ,Young adult ,Cyclophosphamide ,Aged ,Etoposide ,Retrospective Studies ,Salvage Therapy ,Chemotherapy ,Adenine Nucleotides ,business.industry ,Lymphoblastic lymphoma ,Cytarabine ,Off-Label Use ,Hematology ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,medicine.disease ,Lymphoma ,Spain ,Adult Acute Lymphoblastic Leukemia ,Drug Evaluation ,Female ,Arabinonucleosides ,business ,medicine.drug - Abstract
The present study reports the Spanish PETHEMA group experience in 31 heavily pretreated relapsed/refractory acute lymphoblastic leukemia (ALL) and lymphoma (LL) patients treated with clofarabine-based regimens. The complete remission (CR) rate was 31% (median CR duration of 3 months [range 2–28]) and the overall survival probability at 1 year was 10% (95%CI 4–16%). Responses were seen in B and T lineage diseases and in patients with adverse cytogenetics. Hematological and infectious grade >3 toxicities were found in 100 and 67% of the patients, respectively, with 7 (23%) treatment-related deaths. Other organ toxicities were infrequent. Clofarabine-based chemotherapy regimens might induce CRs in ALL and LL patients, but hematological toxicity and infections may limit their use in heavily pretreated patients.
- Published
- 2012
20. Understanding the relationship between attachment style, pain appraisal and illness behavior in women
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Antonio Mundo, Elena Miró, Ana I. Sánchez, Elena Martínez, and M. Pilar Martínez
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medicine.medical_specialty ,Beck Anxiety Inventory ,Chronic pain ,Beck Depression Inventory ,Dysfunctional family ,General Medicine ,Hypervigilance ,medicine.disease ,Arts and Humanities (miscellaneous) ,McGill Pain Questionnaire ,Developmental and Educational Psychology ,medicine ,Attachment theory ,Pain catastrophizing ,medicine.symptom ,Psychology ,Psychiatry ,General Psychology - Abstract
Martinez, M. P., Miro, E., Sanchez, A. I., Mundo, A. & Martinez, E. (2012). Understanding the relationship between attachment style, pain appraisal and illness behavior in women. Scandinavian Journal of Psychology 53, 54–63. Insecure attachment has been hypothesized to be an important factor for understanding the experience of pain. Considering the Attachment-Diathesis Model of Chronic Pain developed by Meredith, Ownsworth, and Strong (2008), this cross-sectional study examines the relationship between attachment style, pain appraisal, and illness behavior. Two hundred healthy women recruited from community contexts completed a battery of self-report measures including the Short-form McGill Pain Questionnaire, Pain Catastrophizing Scale, Pain Anxiety Symptoms Scale-20, Pain Vigilance and Awareness Questionnaire, Illness Attitude Scales, Beck Anxiety Inventory, Beck Depression Inventory, and the Experiences in Close Relationships Questionnaire-Revised. The results showed that attachment anxiety was significantly correlated with pain catastrophizing, pain-related fear, depression, and illness behavior. However, attachment anxiety and avoidance were not associated with pain intensity. Attachment anxiety moderated the relationship between pain catastrophizing and illness behavior, and between pain hypervigilance and illness behavior. Pain catastrophizing and pain-related fear partially mediated the effect of attachment anxiety on illness behavior. The findings highlight potential contributions of attachment style and pain appraisal for explaining illness behavior. This study supports earlier reports and suggests the usefulness of assessing attachment style for early identification of people who might exhibit a high risk of dysfunctional responses to pain. Our findings also suggest that increasing people’s insight about their attachment style and modifying some associated dysfunctional responses may be important in the treatment of chronic pain.
- Published
- 2011
21. Mechanisms involved in endothelin-1-induced contraction of the pig urinary bladder neck
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Albino García-Sacristán, Paz Recio, Medardo Hernández, Luis M. Orensanz, María Victoria Barahona, José Luis Arteaga, Ana Martínez-Sáenz, Vítor S. Fernandes, Dolores Prieto, Ana S. F. Ribeiro, and María Pilar Martínez
- Subjects
Agonist ,medicine.medical_specialty ,Contraction (grammar) ,Voltage-dependent calcium channel ,business.industry ,medicine.drug_class ,Urology ,Pig bladder ,Smooth muscle contraction ,Endothelin 1 ,Endocrinology ,Internal medicine ,Medicine ,Neurology (clinical) ,medicine.symptom ,business ,Endothelin receptor ,Muscle contraction - Abstract
Aims There is no information about the signaling pathways involved in the endothelin-1 (ET-1)-induced contraction of bladder neck. The current study investigates the mechanisms involved in the ET-1-elicited contraction in the pig bladder neck. Methods Bladder neck strips were mounted in organ baths containing physiological saline solution at 37°C and gassed with 95% O2 and 5% CO2, for isometric force recording to endothelin receptor agonists, noradrenaline (NA), and electrical field stimulation. Endothelin ETA receptor expression was also determined, by both immunohistochemistry and Western blot. Results ETA receptor expression (Western blot) was observed in the muscular layer and urothelium. A strong ETA-immunoreactivity (ETA-IR) was identified within nerve fibers among smooth muscle bundles. ET-1 and ET-2 evoked similar concentration-dependent contractions of urothelium-denuded preparations. ET-3 produced a slight response, whereas the ETB receptor agonist BQ3020 failed to promote contraction. BMS182874, an ETA receptor antagonist, reduced ET-1-induced contraction whereas BQ788, an ETB antagonist, did not change such responses. ET-1 contractions were reduced by extracellular Ca2+ removal and by inhibition of voltage-gated Ca2+ (VOC) (L-type) and non-VOC channels, Rho/Rho-kinase pathway, and neuronal VOC channels. NA produced contractions which were enhanced by ET-1 threshold concentrations. ETA receptor blockade enhanced nitric oxide-dependent nerve-mediated relaxations. Conclusions These results suggest that ET-1 produces contraction via muscular ETA receptors coupled to extracellular Ca2+ entry via VOC (L-type) and non-VOC channels. Intracellular Ca2+ mobilization and a Rho/Rho-kinase pathway could also be involved in these responses. ET-1-evoked potentiation on noradrenergic contraction, and neuronal ETA receptors modulating nitrergic inhibitory neurotransmission, are also demonstrated. Neurourol. Urodynam. 31:156–161, 2012. © 2011 Wiley Periodicals, Inc.
- Published
- 2011
22. When is pain related to emotional distress and daily functioning in fibromyalgia syndrome? The mediating roles of self-efficacy and sleep quality
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María Pilar Martínez, A. Medina, Germán Prados, Elena Miró, and Ana I. Sánchez
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Self-efficacy ,Mediation (statistics) ,medicine.medical_specialty ,Dysfunctional family ,Cognition ,General Medicine ,medicine.disease ,Sleep in non-human animals ,Fibromyalgia ,medicine ,Anxiety ,medicine.symptom ,Psychiatry ,Psychology ,Applied Psychology ,Depression (differential diagnoses) ,Clinical psychology - Abstract
Objectives. Fibromyalgia (FM) is a chronic pain syndrome associated with adverse symptoms of cognitive, behavioural, and emotional dysfunction. Accumulating evidence supports the notion that sleep dysfunction, which affects almost all FM patients, has a reciprocal influence on pain. Yet, little is known about the relationship between sleep and other FM symptoms. The present study analysed the role of sleep dysfunction as a mediator of the impact of pain intensity on anxiety, depression, and daily functioning, comparing them with the mediating role of self-efficacy. Design. A cross-sectional design was used. Methods. A sample of 104 women with FM and 86 healthy control women completed a semi-structured interview and self-reported measures of pain, sleep quality, anxiety, depression, and daily functioning. Multiple mediation models were proposed and a bootstrapping approach was used to test these models. Results. Women diagnosed with FM had more dysfunctional scores on the variables examined than control participants, and there were significant relationships between all the variables examined in the mediation models for the FM group. The mediation analyses suggested that sleep quality and self-efficacy significantly mediated the relationship between pain and emotional distress. Additionally, self-efficacy was a significant mediator and sleep quality a likely mediator that was marginally significant in the relationship between pain and functioning. Conclusions. Sleep dysfunction is importantly related to FM symptoms and deserves more attention in both research and clinical practice. Our results suggest that, in addition to the usual treatment of FM, improving sleep could optimize the current management of the syndrome.
- Published
- 2011
23. Adalimumab induction and maintenance therapy for patients with ulcerative colitis previously treated with infliximab
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J.L. Mendoza, Guillermo Bastida, Cristina Saro, S. Garcia-Morán, Jesús Estellés, Elena Gento, Isabel Vera, Ignacio Fernández-Blanco, M. Barreiro-de Acosta, María Chaparro Sánchez, Pilar Martínez-Montiel, Olga Merino, Ignacio Marín-Jiménez, Valle García-Sánchez, Javier P. Gisbert, and Carlos Taxonera
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,Hepatology ,business.industry ,medicine.medical_treatment ,Gastroenterology ,medicine.disease ,Ulcerative colitis ,Infliximab ,Surgery ,Maintenance therapy ,Refractory ,Internal medicine ,Monoclonal ,medicine ,Adalimumab ,Pharmacology (medical) ,Colitis ,skin and connective tissue diseases ,business ,Colectomy ,medicine.drug - Abstract
Aliment Pharmacol Ther 2011; 33: 340–348 Summary Background The long-term efficacy of adalimumab in patients with ulcerative colitis is not well known. Aim To evaluate the short- and long-term outcomes of adalimumab in ulcerative colitis patients previously treated with infliximab. Methods Patients with active ulcerative colitis were treated with adalimumab after failure of other therapies including infliximab. Short-term clinical response and remission were assessed at weeks 4 and 12. The proportion of patients who continued on adalimumab and the proportion of patients who remained colectomy free were assessed over the long term. Results Clinical response at weeks 4 and 12 was achieved in 16 (53%) and 18 (60%) patients, respectively, and clinical remission was obtained in 3 (10%) and 8 (27%) patients, respectively. After a mean 48 weeks’ follow-up, 15 patients (50%) continued on adalimumab. Six patients (20%) required colectomy. All patients who achieved clinical response at week 12 were colectomy free at long term. Conclusions Adalimumab was well tolerated and induced durable clinical response in many patients with otherwise medically refractory ulcerative colitis. Patients achieving clinical response at week 12 avoided colectomy over the long term.
- Published
- 2010
24. Insulin resistance in penile arteries from a rat model of metabolic syndrome
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Sara Benedito, Rafaela Raposo, Belén Climent, Pilar Martínez, Albino García-Sacristán, Ana Sánchez, Dolores Prieto, and Cristina Contreras
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Pharmacology ,medicine.medical_specialty ,Endothelium ,business.industry ,Insulin ,medicine.medical_treatment ,Vasodilation ,medicine.disease ,medicine.anatomical_structure ,Insulin resistance ,Endocrinology ,Internal medicine ,medicine ,Hyperinsulinemia ,Endothelial dysfunction ,medicine.symptom ,Metabolic syndrome ,business ,Vasoconstriction - Abstract
Background and purpose Metabolic and cardiovascular abnormalities accompanying metabolic syndrome, such as obesity, insulin resistance and hypertension, are all associated with endothelial dysfunction and are independent risk factors for erectile dysfunction. The purpose of the present study was to investigate the vascular effects of insulin in penile arteries and whether these effects are impaired in a rat model of insulin resistance and metabolic syndrome. Experimental approach Penile arteries from obese Zucker rats (OZR) and their counterpart, lean Zucker rats (LZR), were mounted on microvascular myographs and the effects of insulin were assessed in the absence and presence of endothelium and of specific inhibitors of nitric oxide (NO) synthesis, phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK). Insulin-induced changes in intracellular Ca 2+ concentration [Ca 2+] i were also examined. KEY RESULTS OZR exhibited mild hyperglycaemia, hypercholesterolemia, hypertryglyceridemia and hyperinsulinemia. Insulin induced endothelium- and NO-dependent relaxations in LZR that were impaired in OZR. Inhibition of PI3K reduced relaxation induced by insulin and by the β-adrenoceptor agonist isoprenaline, mainly in arteries from LZR. Antagonism of endothelin 1 (ET-1) receptors did not alter insulin-induced relaxation in either LZR or OZR, but MAPK blockade increased the responses in OZR. Insulin decreased [Ca 2+] i, a response impaired in OZR. Conclusions and implications Insulin-induced relaxation was impaired in penile arteries of OZR due to altered NO release through the PI3K pathway and unmasking of a MAPK-mediated vasoconstriction. This vascular insulin resistance is likely to contribute to the endothelial dysfunction and erectile dysfunction associated with insulin resistant states. © 2010 The British Pharmacological Society.
- Published
- 2010
25. Altered arachidonic acid metabolism via COX-1 and COX-2 contributes to the endothelial dysfunction of penile arteries from obese Zucker rats
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Alvaro Sanchez, Pilar Martínez, Ana M. Briones, Dolores Prieto, Mercedes Salaices, Ana Cristina Martínez, Medardo Hernández, Cristina Contreras, Nuria Villalba, and Albino García-Sacristán
- Subjects
Pharmacology ,medicine.medical_specialty ,biology ,Endothelium ,Vasodilation ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,Circulatory system ,medicine ,biology.protein ,Cyclooxygenase ,Endothelial dysfunction ,medicine.symptom ,Vasoconstriction ,Blood vessel ,Artery - Abstract
Background and purpose: The aim of the current study was to investigate the role of arachidonic acid (AA) metabolism via cyclooxygenase (COX) in the endothelial dysfunction of penile arteries from pre-diabetic, obese Zucker rats (OZR). Experimental approach: Penile arteries from OZR and from lean Zucker rats (LZR) were mounted in microvascular myographs to assess vascular function and COX expression was determined by immunohistochemistry. Key results: Acetylcholine (ACh) and AA elicited relaxations that were impaired in arteries from OZR. Inhibition of both COX-1 and COX-2 reduced the relaxant effects of ACh and AA in LZR but not in OZR. Inhibitors of COX-1 and of the TXA2/PGH2 (TP) receptor enhanced the relaxations induced by AA in both LZR and OZR, whereas COX-2 inhibition enhanced these responses only in OZR. TP receptor blockade did not restore ACh relaxant responses in arteries from OZR. Inhibition of COX-1 increased basal tension in OZR and this contraction was blunted by TP receptor blockade. The vasoconstrictor responses to noradrenaline were augmented by indomethacin and by COX-2 inhibition in LZR but not in OZR. Immunohistochemical staining showed that both COX-1 and COX-2 are expressed in the endothelium of penile arteries from both LZR and OZR. Conclusions and implications: Vasoactive prostanoids were formed via constitutively active COX-1 and COX-2 pathways in normal rat penile arteries. Under conditions of insulin resistance, the release and/or effects of vasodilator prostanoids were impaired, contributing to the blunted endothelium-dependent vasodilatation and to the enhanced vasoconstriction.
- Published
- 2010
26. Antistaphylococcal Effect of Enterocin AS-48 in Bakery Ingredients of Vegetable Origin, Alone and in Combination with Selected Antimicrobials
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Rosario Lucas López, Pilar Martínez Viedma, Hikmate Abriouel, Nabil Ben Omar, and Antonio Gálvez
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Staphylococcus aureus ,Time Factors ,Colony Count, Microbial ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Foodborne Diseases ,chemistry.chemical_compound ,Anti-Infective Agents ,Bacteriocins ,Bacteriocin ,medicine ,Food microbiology ,Food science ,Antibacterial agent ,Food preservation ,food and beverages ,Drug Synergism ,Antimicrobial ,Cold Temperature ,Eugenol ,chemistry ,Food Microbiology ,Food Preservatives ,Antibacterial activity ,Food Science - Abstract
The inhibitory effect of enterocin AS-48 against Staphylococcus aureus was investigated in various types of bakery ingredients. Antibacterial activity greatly depended on the food substrate, ranging from complete inactivation of S. aureus in liquid caramel (in which the bacterium survived poorly) to no significant inhibition (as in vanilla or chocolate creams). Significant reductions of viable counts in the range of 1.8 to 2.7 log units (P < 0.05) were achieved in substrates like pumpkin confiture or diluted almond cream stored at temperatures of 10 or 22 degrees C. Given the very low activity detected in chocolate substrates, enterocin AS-48 was tested in combination with other antimicrobials. Bactericidal activity increased markedly for the combinations of AS-48 and 0.1% eugenol (v/v), 0.5% 2-nitropropanol (v/v), or 3% Nisaplin (w/v). Enterocin AS-48 could be applied in combination with other antimicrobials for preservation of bakery ingredients against S. aureus.
- Published
- 2009
27. Virtual web sound laboratories as an educational tool in physics teaching in engineering
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J. M. Martínez-Jiménez, G. Pedrós Pérez, M. C. García, Pilar Martínez-Jiménez, Marta Varo, R. Posadillo, and E. P. Varo-Martínez
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General Computer Science ,Multimedia ,Computer Applications ,Computer science ,media_common.quotation_subject ,General Engineering ,computer.software_genre ,Education ,Simulation software ,World Wide Web ,Virtual Laboratory ,Internet servers ,Quality (business) ,University teaching ,computer ,media_common - Abstract
In this article, the use of Virtual Sound Laboratories together with Experimental ones in the university teaching system is explained. The computer programs (http://rabfis15.uco.es/portalsonido/ and http://rabfis15.uco.es/AspectosSonido/) can be used from the online area of Department of Applied Physics Internet server http://www.rabfis15. uco.es/lvct/ in order to be executed via web. These computer applications permit to simulate the speed and properties sound laboratories step by step and in the same way as they are run in the real laboratory, obtaining the corresponding calculations and plots. The aim of these tools is to help students to learn, to study, and to investigate on their own. Furthermore, students can prepare their experiment lessons before going to the laboratory and revise them whenever, and as many times as they want to. Consequently, by using the computer as a complementary educational tool, the quality of university teaching is greatly improved. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 19: 759–769, 2011
- Published
- 2009
28. Impact of Center-related Characteristics and Macroeconomic Factors on the Outcome of Adult Patients With Acute Lymphoblastic Leukemia Treated With Pediatric-inspired Protocols
- Author
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Pere Barba, Mireia Morgades, Pau Montesinos, Jose Gonzalez-Campos, Anna Torrent, Cristina Gil, Teresa Bernal, Mar Tormo, Santiago Mercadal, Sandra Novoa, Irene García-Cadenas, M. Paz Queipo de Llano, Marta Cervera, Rosa Coll, Arancha Bermudez, M. Luz Amigo, Silvia Monsalvo, Jordi Esteve, Raimundo Garcia-Boyero, Andres Novo, Jesús Maria Hernandez Rivas, Antonia Cladera, Pilar Martinez-Sanchez, Josefina Serrano, Maria Teresa Artola, Beatriz Soria, Eugenia Abella, Ferran Vall-Llovera, Juan Bergua, Pilar Herrera, Daniel Barrios, Josep Maria Ribera, and on behalf of the Spanish PETHEMA Group
- Subjects
Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
- Full Text
- View/download PDF
29. Comparison of the MagNA pure LC automated system and the RiboPure-Blood RNA manual method for RNA extraction from multiple myeloma bone marrow samples conserved in an RNA stabilizer
- Author
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Joaquin Martinez-Lopez, S. Gamarra, Pilar Martínez-Sánchez, J. J. Lahuerta, Almudena Crooke, and Guillermo Garcia-Effron
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Biochemistry (medical) ,Clinical Biochemistry ,Extraction (chemistry) ,RNA ,Hematology ,General Medicine ,Biology ,medicine.disease ,Research purpose ,Molecular biology ,medicine.anatomical_structure ,medicine ,Extraction methods ,RNA extraction ,Bone marrow ,Multiple myeloma - Abstract
A total of 62 frozen bone marrow specimens conserved in RNA later (Ambion) were processed using two different extraction methods, the MagNA Pure LC system (MAG; Roche) and the manual RiboPure-Blood RNA method (RIBO; Ambion); Beta glucoronidase RNA (GUS) was amplified by LightCycler PCR to evaluate the quality of both extraction procedures. Less than 1000 GUS copies/ml was detected in 26 of 62 specimens (41.94%) processed by MAG and in five of 62 specimens (8.06%) processed by RIBO. Moreover, RNA recovery from the 62 specimens by MAG is, on average, 2.91 cycle threshold-fold higher than RIBO (P = 0.0008). Furthermore, we compared the extraction times and reagent costs of both methods. In conclusion, RNA extraction using MAG is faster to process 32 samples and less expensive than RIBO but it is not sensitive enough to be employed for research purpose in our laboratory.
- Published
- 2007
30. Heterogeneity of the neuropeptide Y (NPY) contractile and relaxing receptors in horse penile small arteries
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Dolores Prieto, Pilar Martínez, Sara Benedito, Medardo Hernández, Albino García-Sacristán, and Luis Rivera de los Arcos
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Pharmacology ,Agonist ,medicine.medical_specialty ,Contraction (grammar) ,medicine.drug_class ,Vasodilation ,Biology ,Neuropeptide Y receptor ,Receptor antagonist ,humanities ,Endocrinology ,Internal medicine ,Peptide YY ,mental disorders ,medicine ,medicine.symptom ,Receptor ,Vasoconstriction - Abstract
The distribution of neuropeptide Y (NPY)-immunorective nerves and the receptors involved in the effects of NPY upon electrical field stimulation (EFS)- and noradrenaline (NA)-elicited contractions were investigated in horse penile small arteries. NPY-immunoreactive nerves were widely distributed in the erectile tissues with a particularly high density around penile intracavernous small arteries. In small arteries isolated from the proximal part of the corpora cavernosa, NPY (30 nM) produced a variable modest enhancement of the contractions elicited by both EFS and NA. At the same concentration, the NPY Y(1) receptor agonist, [Leu(31), Pro(34)]NPY, markedly potentiated responses to EFS and NA, whereas the NPY Y(2) receptor agonist, NPY(13-36), enhanced exogenous NA-induced contractions. In arteries precontracted with NA, NPY, peptide YY (PYY), [Leu(31), Pro(34)]NPY and the NPY Y(2) receptor agonists, N-acetyl[Leu(28,31)]NPY (24-36) and NPY(13-36), elicited concentration-dependent contractile responses. Human pancreatic polypeptide (hPP) evoked a biphasic response consisting of a relaxation followed by contraction. NPY(3-36), the compound 1229U91 (Ile-Glu-Pro-Dapa-Tyr-Arg-Leu-Arg-Tyr-NH2, cyclic(2,4')diamide) and eventually NPY(13-36) relaxed penile small arteries. The selective NPY Y(1) receptor antagonist BIBP3226 ((R)-N(2)-(diphenacetyl)-N-[(4-hydroxyphenyl)methyl]D-arginineamide) (0.3 microM) shifted to the right the concentration-response curves to both NPY and [Leu(31), Pro(34)]NPY and inhibited the contractions induced by the highest concentrations of hPP but not the relaxations observed at lower doses. In the presence of the selective NPY Y(2) receptor antagonist BIIE0246 ((S)-N2-[[1-[2-[4-[(R,S)-5,11-dihydro-6(6h)-oxodibenz[b,e]azepin-11-y1]-1-piperazinyl]-2-oxoethyl]cyclo-pentyl-N-[2-[1,2-dihydro-3,5 (4H)-dioxo-1,2-diphenyl-3H-1,2, 4-triazol-4-yl]ethyl]-argininamide) (0.3 microM), the Y(2) receptor agonists NPY(13-36) and N-acetyl[Leu(28,31)]NPY (24-36) evoked potent slow relaxations in NA-precontracted arteries, under conditions of nitric oxide (NO) synthase blockade. Mechanical removal of the endothelium markedly enhanced contractions of NPY on NA-precontracted arteries, whereas blockade of the neuronal voltage-dependent Ca(2+) channels did not alter NPY responses. These results demonstrate that NPY can elicit dual contractile/relaxing responses in penile small arteries through a heterogeneous population of postjunctional NPY receptors. Potentiation of the contractions evoked by NA involve both NPY Y(1) and NPY Y(2) receptors. An NO-independent relaxation probably mediated by an atypical endothelial NPY receptor is also shown and unmasked in the presence of selective antagonists of the NPY contractile receptors.
- Published
- 2004
31. NADPH-diaphorase distribution in the rabbit superior colliculus and co-localization with calcium-binding proteins
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Juncal González-Soriano, Pilar Marín‐García, Elisia Rodríguez-Veiga, Pilar Martínez-Sainz, Julio Contreras-Rodríguez, and Susana Martín-Palacios
- Subjects
Calbindins ,Superior Colliculi ,Cell type ,Histology ,Calbindin ,Nitric oxide ,chemistry.chemical_compound ,S100 Calcium Binding Protein G ,Calcium-binding protein ,Neuroplasticity ,Animals ,Distribution (pharmacology) ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Neurons ,biology ,Superior colliculus ,Calcium-Binding Proteins ,NADPH Dehydrogenase ,Original Articles ,Cell Biology ,Cell biology ,Parvalbumins ,Biochemistry ,chemistry ,biology.protein ,Female ,Rabbits ,Anatomy ,Parvalbumin ,Developmental Biology - Abstract
Nitric oxide (NO) and calcium-binding proteins (CaBP) are important neuromodulators implicated in brain plasticity and brain disease. In addition, the mammalian superior colliculus (SC) has one of the highest concentrations of NO within the brain. The present study was designed to determine the distribution of nitric oxide-synthesizing neurons in the SC of the rabbit by enzyme histochemistry for reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), and its degree of co-localization with CaBP, parvalbumin (PV) and calbindin (CB). NADPH-d-labelled fibres formed dense patches of terminal buttons within the intermediate grey layer and streams of fibres within the deepest layers of SC. Cells expressing NOS constitute a subpopulation of neurons in which practically all cell types are represented. Combined PV/NADPH-d experiments showed a complete lack of co-localization within individual neurons and fibres. On the contrary, double-labelled neurons appeared in CB/NADPH-d-stained sections, only in the superficial layers, and mostly in the SGS and SO. These cells, which were intermingled with other neurons containing either NADPH-d or CB, appear to be a subtype of narrow-field and wide-field vertical cells, and display an anterior-posterior gradient of density. Owing to the involvement of the superficial layers of the SC in the organization and integration of the visual information, it is suggested that these neurons may play a concrete role within the visual circuits. Our data indicate a clear selectivity in the expression of NADPH-d, PV and CB in the SC, and that NO and CB probably serve as co-modulators and/or co-transmitters in the connectivity of the superficial layers of this midbrain structure.
- Published
- 2002
32. Local stability in endogenous growth models
- Author
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María Pilar Martínez-García
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State variable ,Control and Optimization ,Endogenous growth theory ,Transversality ,Applied Mathematics ,Topology ,Stability (probability) ,Instability ,Stable manifold ,Manifold ,Control and Systems Engineering ,Saddle point ,Applied mathematics ,Software ,Mathematics - Abstract
The local stability of balanced paths in endogenous growth models is investigated. Under the fulfillment of transversality conditions, a general instability result is established. It has been proved that models with one state variable are completely unstable, whereas two alternatives are possible in models with two states: complete instability or saddle point property. Balanced paths of models with three state variables can either be completely unstable, satisfy the saddle point property with a one-dimensional stable manifold, or have a two-dimensional manifold approaching them. Necessary and sufficient conditions for the saddle point property to occur are given. Transversality conditions guarantee that closed contours and periodic sinuoidal motions around the balanced path are ruled out. Copyright © 2001 John Wiley & Sons, Ltd.
- Published
- 2001
33. Soft-tissue osteosarcoma with prominent aneurysmatic bone cyst-like features: A case report
- Author
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Antonio Jover, Pilar Martínez, and Juan B. Laforga
- Subjects
musculoskeletal diseases ,Pathology ,medicine.medical_specialty ,Histology ,Population ,Bone Neoplasms ,Soft Tissue Neoplasms ,Pathology and Forensic Medicine ,Humans ,Medicine ,Knee ,education ,Bone cyst ,Aged ,Osteosarcoma ,education.field_of_study ,business.industry ,Osteoid ,Cartilage ,General Medicine ,Aneurysmal bone cyst ,Anatomy ,medicine.disease ,Immunohistochemistry ,Bone Cysts, Aneurysmal ,medicine.anatomical_structure ,Giant cell ,Female ,Sarcoma ,business - Abstract
We describe the cytological features of a soft-tissue high-grade spindle and pleomorphic sarcoma. The smears showed hypercellularity composed of pleomorphic round and elongated cells, and a striking population of multinucleated osteoclast-type giant cells. Microscopically the tumor showed multiple patterns such as highly cellular pleomorphic, less cellular, and hyalinized areas with neoplastic cartilage and osteoid, and areas with hemorrhage and multicystic appearance with numerous osteoclastic giant cells, resembling aneurysmal bone cysts. Immunohistochemical studies showed positivity for vimentin, while chondroid tissue was positive for S-100, and osteoclastic giant cells stained positively for CD68. Due to the varied microscopic patterns, the differential diagnosis included many tumors containing osteoclastic giant cells and osteochondroid tissue.
- Published
- 2001
34. Calbindin D28k and parvalbumin immunoreactivity in the rabbit superior colliculus: An anatomical study
- Author
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M. Luisa González-Flores, Juncal González-Soriano, Elisia Rodríguez-Veiga, Julio Contreras-Rodríguez, and Pilar Martínez-Sainz
- Subjects
Neurons ,Calbindins ,Superior Colliculi ,Cell type ,Neuropil ,Morphology (linguistics) ,biology ,Superior colliculus ,Nerve Tissue Proteins ,Anatomy ,Agricultural and Biological Sciences (miscellaneous) ,Parvalbumins ,S100 Calcium Binding Protein G ,medicine.anatomical_structure ,Calcium-binding protein ,biology.protein ,medicine ,Animals ,Calbindin d28k ,Rabbits ,Fluorescent Antibody Technique, Indirect ,Parvalbumin ,Stratum - Abstract
The expression pattern of two calcium binding proteins (CaBP), calbindin D28k (CB) and parvalbumin (PV), in the superior colliculus (SC) of the adult rabbit, as well as the morphology of the immunoreactive cells were examined. The study was performed on 12 rabbits. Coronal sections from postmortem SC were analyzed by light microscopy, and drawings of CaBP-labeled cells were obtained using a drawing tube. No previous information is available on either the CB/PV expression or the morphology of CB/PV positive cells in the SC of the adult rabbit. Therefore, in this study we show that CB neurons and neuropil form three main tiers: the first located within the stratum zonale (SZ) and the upper part of the stratum griseum superficiale (SGS), the second located within the stratum griseum intermedium (SGI), and the third, located within the medial and central areas of the stratum griseum profundum (SGP). In contrast to this layer labeling, almost no CB-positivity is found within the other collicular layers. On the other hand, the densest concentration of PV labeled cells and terminals is found within a single dense tier that spanned the ventral part of the startum griseum superficiale (SGS) and the dorsal part of the stratum opticum (SO). Anti-PV neurons are also scattered through the deeper layers below the dense tier. In contrast, almost no anti-PV labeled neurons or neuropil are found within the stratum zonale (SZ) and upper SGS. This distribution represents a new pattern of sublamination in the SC of this species. All the previously described cell types in other mammals are observed in the rabbit SC: marginal cells, horizontal cells, pyriform cells, narrow-field vertical cells, wide-field vertical cells, and stellate/multipolar cells. Detailed drawings of all these cellular types are represented to show their complete morphology. The results of this study indicate that both CB and PV are present in a variety of neurons, which present a number of homologies between mammals, but have a different location and/or distribution, according to the different species. These findings are thus relevant to better understand the organisation of the SC in mammals.
- Published
- 2000
35. Somatosensory amplification in hypochondriasis and panic disorder
- Author
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M. Pilar Martínez, Cristina Botella, and Amparo Belloch
- Subjects
medicine.medical_specialty ,Panic disorder ,Somatosensory amplification ,Beck Depression Inventory ,Panic ,medicine.disease ,behavioral disciplines and activities ,humanities ,Clinical Psychology ,Minnesota Multiphasic Personality Inventory ,Cronbach's alpha ,Internal consistency ,medicine ,Anxiety ,medicine.symptom ,Psychology ,Psychiatry ,Clinical psychology - Abstract
The aim of this study is to explore the differences and similarities of Somatic Amplification (SA) in both panic and hypochondriasis disorders. An additional objective is to validate the Somatosensory Amplification Scale (SSAS) for use in our cultural context. For these purposes, 34 patients (17 with hypochondriasis and 17 with panic disorder; DSM-III-R criteria) completed the following questionnaires: SSAS, State–Trait Anxiety Inventory, Beck Depression Inventory, MMPI Hypochondriasis Scale, Illness Attitude Scales and Illness Behaviour Questionnaire. The SSAS showed a satisfactory internal consistency (Cronbach's α=0·83), and revealed two components: ‘Internal Stimulus Amplification’ and ‘External Stimulus Amplification’. No significant differences were observed between patients with hypochondriasis and patients with panic disorder on the SSAS. In both groups the SSAS was associated with health concerns (Illness Attitude Scales). The best predictors of SA were Bodily Preoccupation (Illness Attitude Scales) in the hypochondriasis group, and Depression in the panic disorder group. Copyright © 1999 John Wiley & Sons, Ltd.
- Published
- 1999
36. Function of ceramide transfer protein for biogenesis and sphingolipid composition of extracellular vesicles
- Author
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Simone M. Crivelli, Caterina Giovagnoni, Zhihui Zhu, Priyanka Tripathi, Ahmed Elsherbini, Zainuddin Quadri, Jian Pu, Liping Zhang, Branislav Ferko, Dusan Berkes, Stefka D. Spassieva, Pilar Martinez‐Martinez, and Erhard Bieberich
- Subjects
AlphaFold2 ,ceramide ,CERT ,ER‐endosome contact sites ,extracellular vesicles ,HPA‐12 ,Cytology ,QH573-671 - Abstract
Abstract The formation of extracellular vesicles (EVs) is induced by the sphingolipid ceramide. How this pathway is regulated is not entirely understood. Here, we report that the ceramide transport protein (CERT) mediates a non‐vesicular transport of ceramide between the endoplasmic reticulum (ER) and the multivesicular endosome at contact sites. The process depends on the interaction of CERT's PH domain with PI4P generated by PI4KIIα at endosomes. Furthermore, a complex is formed between the START domain of CERT, which carries ceramide, and the Tsg101 protein, which is part of the endosomal sorting complex required for transport (ESCRT‐I). Inhibition of ceramide biosynthesis reduces CERT‐Tsg101 complex formation. Overexpression of CERT increases EV secretion while its inhibition reduces EV formation and the concentration of ceramides and sphingomyelins in EVs. In conclusion, we discovered a function of CERT in regulating the sphingolipid composition and biogenesis of EVs, which links ceramide to the ESCRT‐dependent pathway.
- Published
- 2022
- Full Text
- View/download PDF
37. Stress and health in novice and experienced nursing students
- Author
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Jimenez, Cristobal, primary, Navia‐Osorio, Pilar Martínez, additional, and Diaz, Carmen Vacas, additional
- Published
- 2010
- Full Text
- View/download PDF
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