1. Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation
- Author
-
Simona Fontana, Milena Fini, Angela De Luca, Stefania Pagani, Valeria Carina, Francesca Monteleone, Cesare Faldini, Riccardo Alessandro, Maria Sartori, Stefania Setti, Gianluca Giavaresi, Viviana Costa, Costa, Viviana, Carina, Valeria, Fontana, Simona, De Luca, Angela, Monteleone, Francesca, Pagani, Stefania, Sartori, Maria, Setti, Stefania, Faldini, Cesare, Alessandro, Riccardo, Fini, Milena, Giavaresi, Gianluca, Costa, V., Carina, V., Fontana, S., De Luca, A., Monteleone, F., Pagani, S., Sartori, M., Setti, S., Faldini, C., Alessandro, R., Fini, M., and Giavaresi, G.
- Subjects
Proteomics ,0301 basic medicine ,Time Factors ,Ultrasonic Wave ,Transcription Factor ,Physiology ,Cellular differentiation ,Clinical Biochemistry ,Low-intensity pulsed ultrasound ,Osteogenesis ,Protein Interaction Maps ,Stem Cell Niche ,mesenchymal stem cell ,Cells, Cultured ,Protein metabolic process ,proteomic analysi ,Mesenchymal Stromal Cell ,Reverse Transcriptase Polymerase Chain Reaction ,Osteogenesi ,Intracellular Signaling Peptides and Proteins ,Cell Differentiation ,Osteoblast ,proteomic analysis ,Flow Cytometry ,Cell biology ,RUNX2 ,Phenotype ,medicine.anatomical_structure ,Ultrasonic Waves ,osteoblast differentiation ,osteogenic commitment ,Protein Interaction Map ,Human ,Signal Transduction ,Homeobox protein NANOG ,low-intensity pulsed ultrasound ,Time Factor ,Cell Survival ,Enzyme-Linked Immunosorbent Assay ,Biology ,03 medical and health sciences ,SOX2 ,medicine ,Humans ,Cell Lineage ,Mesenchymal stem cell ,Proteomic ,Mesenchymal Stem Cells ,Cell Biology ,030104 developmental biology ,Gene Expression Regulation ,Intracellular Signaling Peptides and Protein ,Immunology ,Transcription Factors - Abstract
Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6%; OCT-3/4 + /NANOG + /SOX2 + : 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.
- Published
- 2017
- Full Text
- View/download PDF