Search

Your search keyword '"Saada S"' showing total 12 results
Start Over Author "Saada S" Publisher wiley
12 results on '"Saada S"'

11. Oral transforming growth factor-beta receptor 1 inhibitor vactosertib promotes osteosarcoma regression by targeting tumor proliferation and enhancing anti-tumor immunity.

Author

Choi SH, Myers JT, Tomchuck SL, Bonner M, Eid S, Kingsley DT, VanHeyst KA, Kim SJ, Kim BG, and Huang AY

Subjects
Humans, Animals, Receptor, Transforming Growth Factor-beta Type I antagonists & inhibitors, Mice, Bone Neoplasms drug therapy, Bone Neoplasms immunology, Bone Neoplasms pathology, Cell Line, Tumor, Xenograft Model Antitumor Assays, Administration, Oral, Osteosarcoma drug therapy, Osteosarcoma immunology, Cell Proliferation drug effects
Published
2024
Full Text
View/download PDF

12. Effects of ssDNA sequences on non-sequence-specific protein binding.

Author

Mou TC, Shen M, Abdalla S, Delamora D, Bochkareva E, Bochkarev A, and Gray DM

Subjects
Base Composition, Base Pairing, Base Sequence, Binding Sites, Circular Dichroism, DNA-Binding Proteins chemistry, Humans, Models, Molecular, Molecular Sequence Data, Molecular Weight, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Replication Protein A chemistry, Spectrophotometry, Ultraviolet, Viral Proteins chemistry, DNA, Single-Stranded chemistry, DNA-Binding Proteins metabolism, Nucleic Acid Conformation, Replication Protein A metabolism, Viral Proteins metabolism
Abstract

The circular dichroism (CD) spectra of single-stranded DNAs (ssDNAs) are significantly perturbed by the binding of single-stranded DNA binding proteins such as the Ff bacteriophage gene 5 protein (g5p) and the A domain of the 70 kDa subunit of human replication protein A (RPA70-A). These two proteins have similar OB-fold secondary structures, although their CD spectra at wavelengths below 250 nm differ greatly. The spectrum of g5p is dominated by a tyrosyl L(a) band at 229 nm, while that of RPA70-A is dominated by its beta secondary structure. Despite differences in their inherent spectral properties, these two proteins similarly perturb the spectra of bound nucleic acid oligomers. CD spectra of free, non-protein-bound ssDNAs are dependent on interactions of the nearest-neighboring nucleotides in the sequence. The CD spectra (per mol of nucleotide) of simple repetitive sequences 48 nucleotides in length and containing simple combinations of A and C are related by nearest-neighbor equations. For example, 3 x Deltaepsilon[d(AAC)(16)] = 3 x Deltaepsilon[d(ACC)(16)] + Deltaepsilon[d(A)(48)] - Deltaepsilon[d(C)(48)]. Moreover, nearest-neighbor equations relate the spectra of ssDNAs when they are bound by g5p, indicating that each type of perturbed nearest neighbor has a similar average structure within the binding site of the protein.

Published
2006
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results