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BACKGROUND The shortage of organs for transplantation remains a global problem. The retransplantation of a previously transplanted kidney might be a possibility to expand the pool of donors. We provide our experience with the successful reuse of transplanted kidneys in the Eurotransplant region. METHODS A query in the Eurotransplant database was performed between January 1, 1995 and December 31, 2015, to find kidney donors who themselves had previously received a kidney graft. RESULTS 9 out of a total of 68,554 allocated kidneys had previously been transplanted. 4 of these kidneys were transplanted once again. The mean interval between the first transplant and retransplantation was 1689±1682 days (SD; range 55-5,333 days). At the time of the first transplantation the mean serum creatinine of the donors was 1.0 mg/dl (0.6-1.3mg/dl) and at the second transplantation 1.4 mg/dl (0.8- 1.5mg/dl). The mean graft survival in the first recipient was 50 months (2-110 months) and in the second recipient 111 months (40 215 months). CONCLUSION Transplantation of a previously transplanted kidney may successfully be performed with well-preserved graft function and long term graft survival, even if the first transplantation was performed a long time ago. Such organs should be considered even for younger recipients in carefully selected cases. This article is protected by copyright. All rights reserved.

Mass drug administration (MDA) with azithromycin is a core component of the WHO-recommended strategy to eliminate trachoma as a public health problem, but low participation rates in MDA campaigns may undermine the effectiveness of this intervention. We explored factors associated with individual MDA participation at the individual, head of household and household levels in Amhara, Ethiopia.We conducted four district-level, multilevel cluster random coverage surveys to collect data on self-reported MDA participation and predictors. Random-effects logistic regression modelling was used to identify correlates of MDA participation while adjusting for nesting of individuals at the household and village level.The district-level self-reported participation in the trachoma MDA ranged from 78.5% to 86.9%. Excellent and fair health status (Odds ratio [OR] = 5.77; 95% Confidence interval [CI]: 3.04, 10.95; OR = 7.08; 95% CI: 3.47, 14.46), advanced knowledge of the MDA campaign (OR = 2.93; 95% CI: 2.04, 4.21) and knowledge of trachoma (OR = 1.60; 95% CI: 1.17, 2.19) were all positively associated with MDA participation. When excluding heads of household from the model, correlates retained similar positive associations to participation, in addition to the head of household participation (OR = 3.34; 95% CI: 2.46, 4.54).To increase the impact of MDA campaigns, MDA mobilisation strategies-including comprehensive trachoma and azithromycin messaging and MDA campaign awareness-should target heads of household, those in poorer health and older age groups.La distribution en masse de médicaments (DMM) avec l'azithromycine est un élément central de la stratégie recommandée par l’OMS pour éliminer le trachome en tant que problème de santé publique, mais le faible taux de participation aux campagnes de DMM pourrait nuire à l'efficacité de cette intervention. Nous avons exploré les facteurs associés à la participation individuelle à la DMM au niveau de l'individu, du chef de ménage et du ménage à Amhara, en Ethiopie. MÉTHODES: Nous avons mené 4 surveillances de la couverture par grappes, aléatoire, à plusieurs niveaux au niveau du district afin de collecter des données sur la participation auto-déclarée à la DMM et les prédicteurs. Une modélisation par régression logistique à effets aléatoires a été utilisée pour identifier les corrélats de la participation à la DMM tout en ajustant la nidification des individus au niveau du ménage et du village. RÉSULTATS: La participation auto-déclarée au niveau du district à la DMM contre le trachome variait de 78,5% à 86,9%. L’état de santé excellent et passable (rapport de cotes [OR] = 5,77; intervalle de confiance à 95% [IC]: 3,04 -10,95 et OR = 7,08; IC95%: 3,47-14,46), la connaissance poussée sur la campagne de DMM (OR = 2,93; IC95%: 2,04, 4,21) et la connaissance sur le trachome (OR = 1,60; IC95%: 1,17, 2,19) étaient tous positivement associés à la participation à la DMM. En excluant les chefs de ménage du modèle, les corrélats ont conservé des associations positives similaires à la participation, en plus de la participation du chef de ménage (OR = 3,34; IC95%: 2,46, 4,54).Pour accroître l'impact des campagnes de DMM, les stratégies de mobilisation de la DMM, y compris le message complet sur le trachome et l'azithromycine, et la sensibilisation à la campagne de DMM, devraient cibler les chefs de famille, les personnes en mauvaise santé et les groupes de personnes plus âgées.

Background This study fabricated titanium dioxide nanostructures (TDNS) to control broad bean stain virus (BBSV) in faba bean plants. Protection of faba bean against BBSV was evaluated biologically with respect to virus severity, reduction in BBSV accumulation and expression of a pathogenesis-related gene. Results The results indicate that faba bean plants treated with TDNS show a significant reduction in disease severity relative to untreated plants. The regulatory and defense gene involved in the salicylic acid signaling pathway was highly expressed in faba bean plants treated with TDNS compared with untreated plants. The structural features of TDNS, such as the small particle size and suitable shape, contributed to its high efficacy against BBSV. Growth of faba bean plants treated with TDNS was significantly enhanced relative to untreated plants. Conculsion TDNS is an important, eco-friendly and safe strategy for controlling BBSV in faba bean and this study is the first report of this control strategy. © 2018 Society of Chemical Industry.

An ultra-high performance liquid chromatography coupled with tandem mass spectrometry analytical method was developed for simultaneously determining 16 acaricides in citrus based on an optimized quick, easy, cheap, effective, rugged, safe strategy. Good linearities of the standard curve of 5-1000 μg/kg was obtained with regression coefficients higher than 0.9967. Recoveries for all compounds ranged from 72 to 111% with relative standard deviations lower than 14.4% at spiked levels of 5, 50, and 500 μg/kg. Low limits of detection and quantification were readily achieved ranging from 0.05 to 2.7 and 0.10 to 4.3 μg/kg, respectively. Matrix effects were also evaluated for 16 targets with most compounds achieved signal enhancement. Citrus peel gave the highest extent matrix effects, followed by whole citrus and pulp. Finally, this method was successfully applied to detect acaricides residues in real citrus samples. The results showed that pyridaben and quinalphos were the two most frequent and high-concentration compounds with concentrations exceeding the maximum residue limits in five samples, suggesting that the use of these acaricides should be regulated in China in the future.

The effect of an antifungal culture of Lactobacillus plantarum to be used in the storage at refrigeration temperature of fresh black truffles was examined. The strain was selected among 29 lactobacilli isolated from foods and evaluated for their viability and acidification activity at 4 °C, as well as for their inhibitory activity against 11 Penicillium strains isolated from truffles stored at refrigeration temperature. Lb. plantarum 29 showed the ability to hold not only the growth of Penicillium isolated from truffles, but also that of P. digitatum DSM 2750, a green mold involved in the spoilage of truffles. The antifungal activity was observed in vitro and in situ, and the sensory characteristics of truffles were preserved during the cold storage. Practical Application In this study, a strain of Lactobacillus plantarum recognized as a safe microorganism, (generally regarded as safe [GRAS]), was selected for its ability to inhibit the growth of spoilage molds belonging to Penicillium spp. in fresh black truffles. The use of this lactic acid bacterium, alone or in association with other protective cultures, could bring different benefits, such as shelf life extension of fresh truffles by avoiding spoilage due to molds, and replacement of chemical preservatives with a natural and safe strategy.

In 2001, one of the authors wrote in the Medical Journal of Australia about trachoma explaining that Australia is the only developed country in the world where blinding trachoma still exists. Ten years later, the same is still true, but there is some progress and the situation in Australia is starting to change. However, this progress has been somewhat glacial, especially compared with the rapid changes being made in other 'undeveloped' parts of the world.

Background: Increasing the proportion of births with skilled attendance is advocated by international agencies as a key factor in reducing maternal and perinatal mortality and morbidity. The SAFE Strategy Development Tool is designed to enable policy makers and planners to gather and interpret information systematically to develop strategies for improving skilled attendance at birth. Method: Five modules were developed with partners in Bangladesh Ghana Jamaica Malawi and Mexico to guide the identification of problems related to skilled attendance the collection of primary and secondary evidence and the synthesis of this evidence to formulate strategies. The involvement of key players including policy makers is emphasized throughout the application of the tool and is vital to its success. Results: The SAFE Strategy Development Tool was field tested in five collaborating countries. The methods employed by this tool were found to be feasible and produced evidence that will be useful in the formulation of strategies. Application of the tool can be completed in 3 to 5 months and was estimated to cost between US$12938 and US$15627 for applications at district or subdistrict level. The final strategy options developed from the findings were presented at an international workshop in Aberdeen Scotland in February 2003. Conclusion: The SAFE Strategy Development Tool is now available to governments organizations and institutions involved in the implementation of maternal health programs. (authors)