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PCR, or “polymerase chain reaction”, is one of most useful techniques in genomics research. High quality DNA extraction is necessary to obtain satisfactory results. Some methods utilized to obtain DNA from blood include a simple salting-out procedure for extracting DNA from human nucleated cells. This method is widely used by many laboratories because it can obtain DNA with sufficient quality to realize PCR, using materials that do not pose risk to researchers and is more cost-efficient than other approaches. Although salting-out has spread to many laboratories, its protocols do not highlight technical details of their steps that could affect final DNA quality and, for this reason, underwent some modifications in this work in order to obtain an efficient extraction. We used 2 to 4 mL of blood for each of 30 DNA samples. For the supernatant step in first EDTA wash, we established a subtraction pattern from 15 mL to 2.5 mL, independently of initial sample volume of blood, to avoid losing leucocytes. The num...

Amphiregulin (AREG) is considered an oncogene whose expression is related to several types of cancer. Our group identified a triiodothyronine (T3) action on different genes of breast cancer cell li...

This study aimed evaluate whether estrogen modulates amphiregulin (AREG) gene expression in breast tumor cells. For this, MCF-7 cells were treated for 1 or 4 hours with estrogen (E2 10−7 M) in the ...