1. Electrochromatographic behavior of silica monolithic capillaries of different skeleton sizes synthesized with a simplified and shortened sol–gel procedure
- Author
-
D. Brunel, Julien Iapichella, Claire Demesmay, J. L. Rocca, Anne Galarneau, Guillaume Puy, Sciences Analytiques (SA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)
- Subjects
Materials science ,Clinical Biochemistry ,Silica Gel ,010501 environmental sciences ,01 natural sciences ,Biochemistry ,Buffer (optical fiber) ,Polyethylene Glycols ,Analytical Chemistry ,law.invention ,Electrochromatography ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Capillary Electrochromatography ,law ,Phase (matter) ,Calcination ,Sol-gel process ,Monolith ,0105 earth and related environmental sciences ,Sol-gel ,geography ,Capillary electrochromatography ,geography.geographical_feature_category ,Chromatography ,010401 analytical chemistry ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Silicon Dioxide ,0104 chemical sciences ,Ionic strength ,Xanthines ,Solvents ,Nonlinear electroosmosis ,Electroosmosis ,Silica monolithic capillary - Abstract
Silica monolithic capillaries (SMCs) were synthesized by a sol-gel process. First, a simplification of the synthesis was proposed by replacing the calcination and the drying steps which can have tremendous effects on chromatographic and physical properties, by a single water or methanol 2 h washing step. The efficiency of such a washing step was demonstrated and the comparison of the chromatographic and electrochromatographic properties between calcined and washed SMCs has shown that such a modification did not impair retention, efficiency, and stability of the monolith. This simplified procedure was carried out to synthesize SMCs with two different skeleton sizes. These capillaries were evaluated in electrochromatography and present high efficiencies (H = 5 microm) at least equal to the best ones reported in the literature. Furthermore, the influence of the skeleton size on the EOF of the second kind (EOF-2) was investigated with unmodified SMCs used under various experimental conditions including electrical field strength and buffer concentration. The ionic strength of the mobile phase and the applied electrical field that enable this EOF-2 were related to the size of the skeleton which was tuned by the synthesis conditions.
- Published
- 2006
- Full Text
- View/download PDF