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Your search keyword '"Sheele, Johnathan M."' showing total 7 results
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3. Human immunoglobulin G responses toCimex lectulariusL. saliva

Author

Sheele, Johnathan M., primary, Ferrari, Brian, additional, Goddard, Jerome, additional, Schlatzer, Danie, additional, Lundberg, Kathleen C., additional, Guinto, Katirina, additional, Embers, Monica E., additional, Young, Andrew B., additional, Ridge, Gale E., additional, Damiani, Giovanni, additional, and McCormick, Thomas S., additional

Published
2020
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4. Emergency Medicine Resident Assessment of the Emergency Ultrasound Milestones and Current Training Recommendations

Author

Stolz, Lori A., primary, Stolz, Uwe, additional, Fields, J. Matthew, additional, Saul, Turandot, additional, Secko, Michael, additional, Flannigan, Matthew J., additional, Sheele, Johnathan M., additional, Rifenburg, Robert P., additional, Weekes, Anthony J., additional, Josephson, Elaine B., additional, Bedolla, John, additional, Resop, Dana M., additional, dela Cruz, Jonathan, additional, Boysen‐Osborn, Megan, additional, Caffery, Terrell, additional, Derr, Charlotte, additional, Bengiamin, Rimon, additional, Chiricolo, Gerardo, additional, Backlund, Brandon, additional, Heer, Jagdipak, additional, Hyde, Robert J., additional, and Adhikari, Srikar, additional

Published
2017
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7. Human immunoglobulin G responses to Cimex lectularius L. saliva.

Author

Sheele JM, Ferrari B, Goddard J, Schlatzer D, Lundberg KC, Guinto K, Embers ME, Young AB, Ridge GE, Damiani G, and McCormick TS

Subjects
Adolescent, Adult, Animals, Female, Humans, Immunoglobulin G blood, Insect Bites and Stings blood, Male, Middle Aged, Saliva chemistry, Salivary Glands chemistry, Salivary Proteins and Peptides analysis, Salivary Proteins and Peptides immunology, Young Adult, Bedbugs immunology, Immunoglobulin G immunology, Insect Bites and Stings immunology, Saliva immunology
Abstract

Aims: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L., Methods and Results: Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract., Conclusions: Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract., (© 2020 John Wiley & Sons Ltd.)

Published
2020
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