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Your search keyword '"Stegmann, Evi"' showing total 11 results
Start Over Author "Stegmann, Evi" Publisher wiley
11 results on '"Stegmann, Evi"'

2. Genetic Engineering in Combination with Semi‐Synthesis Leads to a New Route for Gram‐Scale Production of the Immunosuppressive Natural Product Brasilicardin A

Author

Botas, Alma, primary, Eitel, Michael, additional, Schwarz, Paul N., additional, Buchmann, Anina, additional, Costales, Paula, additional, Núñez, Luz Elena, additional, Cortés, Jesús, additional, Morís, Francisco, additional, Krawiec, Michał, additional, Wolański, Marcin, additional, Gust, Bertolt, additional, Rodriguez, Mirna, additional, Fischer, Wolf‐Nicolas, additional, Jandeleit, Bernd, additional, Zakrzewska‐Czerwińska, Jolanta, additional, Wohlleben, Wolfgang, additional, Stegmann, Evi, additional, Koch, Pierre, additional, Méndez, Carmen, additional, and Gross, Harald, additional

Published
2021
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3. A novel LysR‐type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin

Author

Wolański, Marcin, primary, Krawiec, Michał, additional, Schwarz, Paul N., additional, Stegmann, Evi, additional, Wohlleben, Wolfgang, additional, Buchmann, Anina, additional, Gross, Harald, additional, Eitel, Michael, additional, Koch, Pierre, additional, Botas, Alma, additional, Méndez, Carmen, additional, Núñez, Luz Elena, additional, Morís, Francisco, additional, Cortés, Jesus, additional, and Zakrzewska‐Czerwińska, Jolanta, additional

Published
2020
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7. Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365

Author

Iftime, Dumitrita, Jasyk, Martin, Kulik, Andreas, Imhoff, Johannes F., Stegmann, Evi, Wohlleben, Wolfgang, Süssmuth, Roderich D., Weber, Tilmann, Iftime, Dumitrita, Jasyk, Martin, Kulik, Andreas, Imhoff, Johannes F., Stegmann, Evi, Wohlleben, Wolfgang, Süssmuth, Roderich D., and Weber, Tilmann

Abstract

Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.

Published
2015
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