10 results on '"Wenwen Huang"'
Search Results
2. Retrospective analysis of the efficacy of adjuvant cytokine‐induced killer cell immunotherapy combined with chemotherapy in colorectal cancer patients after surgery
- Author
-
Xiao Li, Haodong Zhou, Wenwen Huang, Xuejuan Wang, Mingyao Meng, Zongliu Hou, Liwei Liao, Weiwei Tang, Yanhua Xie, Ruotian Wang, Haidong Yu, Liqiong Wang, Huirong Zhu, Wenju Wang, Jing Tan, and Ruhong Li
- Subjects
adjuvant immunotherapy ,colorectal cancer ,cytokine‐induced killer cells ,prognosis ,signalling lymphocytes activating molecule family 7 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Objectives Even though postoperative chemotherapy can eliminate residual tumor cells in patients with colorectal cancer (CRC), severe adversity, weakened immunity and drug resistance are still problems. Adjuvant cytokine‐induced killer (CIK) cell therapy is an alternative to CRC patients after surgery. The present study investigated the efficacy of adjuvant CIK cell therapy combined with chemotherapy in postoperative CRC patients. Methods This retrospective analysis included 137 postoperative CRC patients, including 71 who received adjuvant chemotherapy alone (control group) and 66 who received adjuvant immunotherapy based on CIK cells combined with chemotherapy (CIT group). Results Long‐term follow‐up study indicated that overall survival (OS) and progression‐free survival (PFS) were significantly longer in the CIT group than in the control group. Subgroup analyses showed that CIT treatment significantly improved OS and PFS of CRC patients classified as stage II and N0 stage and in patients with primary tumors in the rectum. Increasing the number of CIK infusions resulted in better prognosis. CRC patients aged
- Published
- 2022
- Full Text
- View/download PDF
3. Reduced m6A modification predicts malignant phenotypes and augmented Wnt/PI3K‐Akt signaling in gastric cancer
- Author
-
Cheng Zhang, Mengqi Zhang, Sai Ge, Wenwen Huang, Xiaoting Lin, Jing Gao, Jifang Gong, and Lin Shen
- Subjects
gastric cancer ,m6A ,tumor suppressor ,Wnt/PI3K‐Akt signaling ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background As the most abundant epigenetic modification on mRNAs and long non‐coding RNAs, N6‐methyladenosine (m6A) modification extensively exists in mammalian cells. Controlled by writers (methyltransferases), readers (signal transducers), and erasers (demethylases), m6A influences mRNA structure, maturation, and stability, thus negatively regulating protein expression in a post‐translational manner. Nevertheless, current understanding of m6A's roles in tumorigenesis, especially in gastric cancer (GC) remains to be unveiled. In this study, we assessed m6A's clinicopathological relevance to GC and explored the underlying mechanisms. Methods By referring to a proteomics‐based GC cohort we previously generated and the TCGA‐GC cohort, we merged expressions of canonical m6A writers (METTL3/METTL14), readers (YTHDF1/YTHDF2/YTHDF3), and erasers (ALKBH5/FTO), respectively, as W, R, and E signatures to represent m6A modification. We stratified patients according to these signatures to decipher m6A's associations with crucial mutations, prognosis, and clinical indexes. m6A's biological functions in GC were predicted by gene set enrichment analysis (GSEA) and validated by in vitro experiments. Results We discovered that W and R were potential tumor suppressive signatures, while E was a potential oncogenic signature in GC. According to W/R/E stratifications, patients with low m6A‐indications were accompanied with higher mutations of specific genes (CDH1, AR, GLI3, SETBP1, RHOA, MUC6, and TP53) and also demonstrated adverse clinical outcomes. GSEA suggested that reduced m6A was correlated with oncogenic signaling and phenotypes. Through in vitro experiments, we proved that m6A suppression (represented by METTL14 knockdown) promoted GC cell proliferation and invasiveness through activating Wnt and PI3K‐Akt signaling, while m6A elevation (represented by FTO knockdown) reversed these phenotypical and molecular changes. m6A may also be involved in interferon signaling and immune responses of GC. Conclusions Our work demonstrated that low‐m6A signatures predicted adverse clinicopathological features of GC, while the reduction of RNA m6A methylation activated oncogenic Wnt/PI3K‐Akt signaling and promoted malignant phenotypes of GC cells.
- Published
- 2019
- Full Text
- View/download PDF
4. DNA methylation‐mediated silencing of microRNA‐204 enhances T cell acute lymphoblastic leukemia by up‐regulating MMP‐2 and MMP‐9 via NF‐κB
- Author
-
Luhui Lin, Dabing Chen, Wenwen Huang, Congmeng Lin, Tingting Xiao, Jingjing Xu, Ting Yang, Haojie Zhu, Dandan Lin, and Dayi Lin
- Subjects
0301 basic medicine ,T cell ,Bisulfite sequencing ,Apoptosis ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ,IRAK1 ,Mice ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,medicine ,Animals ,Humans ,Gene silencing ,Gene Silencing ,T cell acute lymphoblastic leukaemia ,3' Untranslated Regions ,Cell Proliferation ,DNA methylation ,Gene Expression Regulation, Leukemic ,Cell growth ,Chemistry ,NF‐κB ,NF-kappa B ,Promoter ,Original Articles ,Cell Biology ,miR‐204 ,Disease Models, Animal ,MicroRNAs ,Interleukin-1 Receptor-Associated Kinases ,030104 developmental biology ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,030220 oncology & carcinogenesis ,Cancer research ,Matrix Metalloproteinase 2 ,Molecular Medicine ,Female ,RNA Interference ,Original Article - Abstract
T cell acute lymphoblastic leukaemia (T‐ALL) is a highly aggressive haematological cancer of the bone marrow. The abnormal expression of microRNAs (miRNAs) is reportedly involved in T‐ALL development and progression. Thus, we aimed to decipher the involvement of miR‐204 silencing mediated by DNA methylation in the occurrence of T cell acute lymphoblastic leukaemia (T‐ALL). miR‐204 expression was determined in bone marrow and peripheral blood samples from T‐ALL patients by real‐time quantitative PCR (RT‐qPCR) with its effect on cell proliferation evaluated by functional assays. In addition, bisulphite sequencing PCR was employed to detect the DNA methylation level of the miR‐204 promoter region, and the binding site between miR‐204 and IRAK1 was detected by luciferase assay. We found that miR‐204 was down‐regulated in T cells of T‐ALL patients, which was caused by the increased DNA methylation in the promoter region of miR‐204. Moreover, overexpression of miR‐204 inhibited T‐ALL cell proliferation while enhancing their apoptosis through interleukin receptor‐associated kinase 1 (IRAK1), which enhanced the expression of matrix metalloproteinase‐2 (MMP‐2) and MMP‐9 through activation of p‐p65. Thus, miR‐204 modulated MMP‐2 and MMP‐9 through IRAK1/NF‐κB signalling pathway, which was confirmed by in vivo assay. Taken together, DNA methylation‐mediated miR‐204 silencing increased the transcription of IRAK1, thus activating the NF‐κB signalling pathway and up‐regulating the downstream targets MMP‐2/MMP‐9.
- Published
- 2021
5. Effect of Terminal Modification on the Molecular Assembly and Mechanical Properties of Protein-Based Block Copolymers
- Author
-
Matthew M. Jacobsen, Olena Tokareva, Joyce Wong, Markus J. Buehler, Marc Simon, David Li, David L. Kaplan, Davoud Ebrahimi, Cristian Staii, Wenwen Huang, Nina Dinjaski, Shengjie Ling, Massachusetts Institute of Technology. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology. Department of Materials Science and Engineering, Massachusetts Institute of Technology. Department of Physics, MIT Kavli Institute for Astrophysics and Space Research, Tokareva, Olena, Ebrahimi, Davoud, Ling, Shengjie, Dinjaski, Nina, Buehler, Markus J, Kaplan, David L, and Wong, Joyce Y.
- Subjects
0301 basic medicine ,Models, Molecular ,Toughness ,Materials science ,Polymers and Plastics ,Polymers ,Bioengineering ,Peptide ,02 engineering and technology ,Extensibility ,Article ,Core domain ,Biomaterials ,03 medical and health sciences ,Materials Chemistry ,Copolymer ,Computer Simulation ,Composite material ,Spinning ,Mechanical Phenomena ,chemistry.chemical_classification ,Tandem ,021001 nanoscience & nanotechnology ,030104 developmental biology ,chemistry ,Biophysics ,0210 nano-technology ,Peptides ,Hydrophobic and Hydrophilic Interactions ,Biotechnology - Abstract
Accurate prediction and validation of the assembly of bioinspired peptide sequences into fibers with defined mechanical characteristics would aid significantly in designing and creating materials with desired properties. This process may also be utilized to provide insight into how the molecular architecture of many natural protein fibers is assembled. In this work, computational modeling and experimentation are used in tandem to determine how peptide terminal modification affects a fiber-forming core domain. Modeling shows that increased terminal molecular weight and hydrophilicity improve peptide chain alignment under shearing conditions and promote consolidation of semicrystalline domains. Mechanical analysis shows acute improvements to strength and elasticity, but significantly reduced extensibility and overall toughness. These results highlight an important entropic function that terminal domains of fiber-forming peptides exhibit as chain alignment promoters, which ultimately has notable consequences on the mechanical behavior of the final fiber products., National Institutes of Health (U.S.) (U01 EB014976), Texas Advanced Computing Center (grant number TG-DMR140101), Texas Advanced Computing Center (grant number TG-MSS090007)
- Published
- 2017
6. Influence of Solution Parameters on Phase Diagram of Recombinant Spider Silk-Like Block Copolymers
- Author
-
Peggy Cebe, David L. Kaplan, Wenwen Huang, and Sreevidhya Krishnaji
- Subjects
Ammonium sulfate ,Materials science ,Morphology (linguistics) ,Polymers and Plastics ,Organic Chemistry ,Condensed Matter Physics ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,Polymer chemistry ,Materials Chemistry ,Copolymer ,Recombinant DNA ,Spider silk ,Physical and Theoretical Chemistry ,Phase diagram - Published
- 2014
7. High Throughput Screening of Dynamic Silk-Elastin-Like Protein Biomaterials
- Author
-
Qiaobing Xu, David L. Kaplan, Yinan Lin, Xiao-Xia Xia, Qin Wang, and Wenwen Huang
- Subjects
Biomaterials ,Materials science ,SILK ,Characterization methods ,Stimuli responsive ,High-throughput screening ,Electrochemistry ,Nanotechnology ,Computational biology ,Condensed Matter Physics ,Article ,Electronic, Optical and Magnetic Materials - Abstract
The need for dynamic, elastomeric polymeric biomaterials remains high, with few options with tunable control of mechanical properties, and environmental responses. Yet the diversity of these types of protein polymers pursued for biomaterials-related needs remains limited. Robust high-throughput synthesis and characterization methods will address the need to expand options for protein-polymers for a range of applications. To address this need, a combinatorial library approach with high throughput screening is used to select specific examples of dynamic protein silk-elastin-like polypeptides (SELPs) with unique stimuli responsive features, including tensile strength, and adhesion. Using this approach 64 different SELPs with different sequences and molecular weights are selected out of over 2,000 recombinant E. coli colonies. New understanding of sequence-function relationships with this family of proteins is gained through this combinatorial-screening approach and can provide a guide to future library designs. Further, this approach yields new families of SELPs to match specific material functions.
- Published
- 2014
8. Fabrication and Characterization of Recombinant Silk‐Elastin‐Like‐Protein (SELP) Fiber
- Author
-
Jingjie Yeo, Erin G. Roberts, Joyce Wong, Markus J. Buehler, Nae Gyune Rim, David L. Kaplan, Wenwen Huang, Anna Tarakanova, and Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
- Subjects
0301 basic medicine ,Materials science ,Polymers and Plastics ,Silk ,Gene Expression ,Biocompatible Materials ,Bioengineering ,macromolecular substances ,02 engineering and technology ,Molecular Dynamics Simulation ,Article ,law.invention ,Biomaterials ,03 medical and health sciences ,law ,Tensile Strength ,Materials Testing ,Escherichia coli ,Materials Chemistry ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Fiber ,Spinning ,chemistry.chemical_classification ,biology ,Transition temperature ,fungi ,Hydrogels ,Hydrogen Bonding ,Polymer ,Bombyx ,021001 nanoscience & nanotechnology ,Elasticity ,Recombinant Proteins ,Elastin ,030104 developmental biology ,SILK ,Chemical engineering ,chemistry ,Recombinant DNA ,biology.protein ,Protein Conformation, beta-Strand ,Swelling ,medicine.symptom ,0210 nano-technology ,Biotechnology - Abstract
Silk-elastin-like-protein polymers (SELPs) are genetically engineered recombinant protein sequences consisting of repeating units of silk-like and elastin-like blocks. By combining these entities, it is shown that both the characteristic strength of silk and the temperature-dependent responsiveness of elastin can be leveraged to create an enhanced stimuli-responsive material. It is hypothesized that SELP behavior can be influenced by varying the silk-to-elastin ratio. If the responsiveness of the material at different ratios is significantly different, this would allow for the design of materials with specific temperature-based swelling and mechanical properties. This study demonstrates that SELP fiber properties can be controlled via a temperature transition dependent on the ratio of silk-to-elastin in the material. SELP fibers are experimentally wet spun from polymers with different ratios of silk-to-elastin and conditioned in either a below or above transition temperature (T t ) water bath prior to characterization. The fibers with higher elastin content showed more stimuli-responsive behavior compared to the fibers with lower elastin content in the hot (57-60 °C) versus cold (4-7 °C) environment, both computationally and experimentally. This work builds a foundation for developing SELP materials with well-characterized mechanical properties and responsive features.
- Published
- 2018
9. Charge-Tunable Autoclaved Silk-Tropoelastin Protein Alloys That Control Neuron Cell Responses
- Author
-
David L. Kaplan, Xiao Hu, Wenwen Huang, Min D. Tang-Schomer, Anthony S. Weiss, and Xiao-Xia Xia
- Subjects
chemistry.chemical_classification ,Tropoelastin ,biology ,Integrin ,technology, industry, and agriculture ,Fibroin ,Biomaterial ,Nanotechnology ,Polymer ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Extracellular matrix ,SILK ,chemistry ,Tissue engineering ,Electrochemistry ,biology.protein ,Biophysics - Abstract
Tunable protein composites are important for constructing extracellular matrix mimics of human tissues with control of biochemical, structural, and mechanical properties. Molecular interaction mechanisms between silk fibroin protein and recombinant human tropoelastin, based on charge, are utilized to generate a new group of multifunctional protein alloys (mixtures of silk and tropoelastin) with different net charges. These new biomaterials are then utilized as a biomaterial platform to control neuron cell response. With a +38 net charge in water, tropoelastin molecules provide extraordinary elasticity and selective interactions with cell surface integrins. In contrast, negatively charged silk fibroin protein (net charge -36) provides remarkable toughness and stiffness with morphologic stability in material formats via autoclaving-induced beta-sheet crystal physical crosslinks. The combination of these properties in alloy format extends the versatility of both structural proteins, providing a new biomaterial platform. The alloys with weak positive charges (silk/tropoelastin mass ratio 75/25, net charge around +16) significantly improved the formation of neuronal networks and maintained cell viability of rat cortical neurons after 10 days in vitro. The data point to these protein alloys as an alternative to commonly used poly-L-lysine (PLL) coatings or other charged synthetic polymers, particularly with regard to the versatility of material formats (e.g., gels, sponges, films, fibers). The results also provide a practical example of physically designed protein materials with control of net charge to direct biological outcomes, in this case for neuronal tissue engineering.
- Published
- 2013
10. Nanocomposites of poly(vinylidene fluoride) with multiwalled carbon nanotubes
- Author
-
Peggy Cebe, Jenna Woodburn, Shabnam Razmpour, Luis Fernandez, Wenwen Huang, and Kyle Edenzon
- Subjects
Nanocomposite ,Materials science ,Polymers and Plastics ,Concentration effect ,General Chemistry ,Carbon nanotube ,Dynamic mechanical analysis ,Surfaces, Coatings and Films ,law.invention ,Crystallinity ,law ,Materials Chemistry ,Thermal stability ,Composite material ,Crystallization ,Glass transition - Abstract
The preparation and characterization of nanocomposites of poly(vinylidene fluoride), PVDF, with acid treated multiwalled carbon nanotubes (MWCNT) with a wide composition range, from 0.1 to 5.0% MWCNT by weight, is reported. Effect of uniaxial orientation by zone drawing on these nanocomposites is discussed and compared with unoriented compression molded films. Static room temperature two-dimensional wide angle X-ray scattering and Fourier transform infrared spectros- copy were used for phase identification. Differential scan- ning calorimetry, polarizing optical microscopy, dynamic mechanical analysis (DMA), and thermogravimetic analy- sis (TGA) were used to study the thermal and mechanical properties. Incorporation of MWCNT into PVDF has no obvious effect in forming beta phase crystal in the PVDF/ MWCNT bulk films, while zone drawing cause a signifi- cant alpha to beta transition in PVDF/MWCNT. Results indicate that MWCNTs act as nucleation agent during crystallization and slightly increase the degree of crystal- linity of PVDF/MWCNT bulk films. TGA indicates the thermal stability is improved when MWCNT concentration increases for unoriented PVDF/MWCNT film. The modu- lus also increases significantly when MWCNT concentra- tion increases. The glass transition temperature measured by the peak position of tand from DMA does not change with MWCNT concentration, but a slightly higher glass transition can be obtained by zone drawing. V C 2009 Wiley
- Published
- 2010
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.