24 results on '"Xiao Li Liu"'
Search Results
2. Imatinib compared with second‐generation tyrosine kinase‐inhibitors in persons with chronic myeloid leukemia presenting in accelerated phase
- Author
-
Sen Yang, Xiaoshuai Zhang, Robert Peter Gale, Xin Du, Chun‐yan Chen, Jian‐yu Weng, Jian Huang, Fei Li, Yun Zeng, Zhen Xiao, Jian‐da Hu, Li‐jie Yang, Zhuo‐gang Liu, Guo‐hui Li, Xiu‐li Sun, Wei Yang, Ru Feng, Yan‐qiu Han, Yu Jing, Na Xu, Xiao‐li Liu, Zhen‐fang Liu, Xiao‐dong Wang, Shi‐xin Wu, Rong Liang, Yan‐li Zhang, Yun‐fan Yang, Huan‐ling Zhu, Ling Pan, Li Meng, Yan‐hong Zhao, Hai Yi, Yi‐lan Liu, Wei‐hua Zhang, Yuan‐jun Zheng, Ze‐ping Zhou, Su‐ning Chen, Hui‐ying Qiu, Wei‐ming Li, Zhi‐lin Jia, Yan‐liang Bai, Li‐e Lin, Bing‐cheng Liu, Chun‐shui Liu, Jian‐min Luo, Jun‐xia Meng, Zhi‐qiang Sun, Yan‐qing Zhang, Xiao‐jun Huang, and Qian Jiang
- Subjects
Hematology - Published
- 2023
3. <scp>ETS</scp> 1 and <scp>SP</scp> 1 drive <scp>DHX</scp> 15 expression in acute lymphoblastic leukaemia
- Author
-
Jing Wang, Xiao-Li Liu, Qiao Liu, Lili Pan, Yuan Chen, Yang Li, Yuanhua Cai, Jing-Gang Li, Xianglei Chen, Shao-Yuan Wang, Xiaofan Li, and Shui-Ling Shi
- Subjects
0301 basic medicine ,DHX15 ,Transcription, Genetic ,Sp1 Transcription Factor ,ETS1 ,Biology ,Proto-Oncogene Protein c-ets-1 ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Cell Line, Tumor ,Transcriptional regulation ,Humans ,RNA, Messenger ,Promoter Regions, Genetic ,Base Pairing ,Transcription factor ,Gene ,Gene knockdown ,Binding Sites ,promoter ,Base Sequence ,Gene Expression Regulation, Leukemic ,Promoter ,Original Articles ,Cell Biology ,DNA Methylation ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Molecular biology ,SP1 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Molecular Medicine ,CpG Islands ,Original Article ,Transcription Initiation Site ,Chromatin immunoprecipitation ,RNA Helicases ,Protein Binding - Abstract
DHX15 plays a role in leukaemogenesis and leukaemia relapse. However, the mechanism underlying the transcriptional regulation of DHX15 in ALL has not been elucidated. Our present study aimed to explore the functional promoter region of DHX15 and to investigate the transcription factors controlling the transcription of this gene. A luciferase assay performed with several truncated constructs identified a 501‐bp region as the core promoter region of DHX15. Site‐directed mutagenesis, electrophoretic mobility shift and chromatin immunoprecipitation assays showed that ETS1 and SP1 occupied the DHX15 promoter. Furthermore, knockdown of ETS1 and SP1 resulted in suppression of DHX15, whereas the overexpression of these genes led to up‐regulation of DHX15. Interestingly, in samples obtained from patients with ALL at diagnosis, both ETS1 and SP1 correlated positively with DHX15 expression. Additionally, differences in methylation of the DHX15 core promoter region were not observed between the patients and controls. In conclusion, we identified the core promoter region of DHX15 and demonstrated that ETS1 and SP1 regulated DHX15 expression in ALL.
- Published
- 2018
4. Proline-rich transmembrane protein 2- negative paroxysmal kinesigenic dyskinesia: Clinical and genetic analyses of 163 patients
- Author
-
Xiao-Li Liu, Xiao-Rong Liu, Yang-Qi Xu, Hui-Dong Tang, Xiao-Jun Huang, Xiao-Meng Yin, Xia-Nan Guo, Qing Liu, Sheng Zeng, Mei Zhang, Wo-Tu Tian, Sheng-Di Chen, Li Cao, Xiao Mao, Jun-Yi Shen, Beisha Tang, and Wei-Guo Tang
- Subjects
0301 basic medicine ,Genetics ,Proband ,Sanger sequencing ,Movement disorders ,business.industry ,Paroxysmal dyskinesia ,Solute carrier family ,03 medical and health sciences ,Proline-Rich Transmembrane Protein 2 ,symbols.namesake ,030104 developmental biology ,0302 clinical medicine ,Neurology ,Dyskinesia ,medicine ,symbols ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Exome sequencing - Abstract
Background Paroxysmal kinesigenic dyskinesia is the most common type of paroxysmal dyskinesia. Approximately half of the cases of paroxysmal kinesigenic dyskinesia worldwide are attributable to proline-rich transmembrane protein 2 mutations. Objective The objective of this study was to investigate potential causative genes and clinical characteristics in proline-rich transmembrane protein 2-negative patients with paroxysmal kinesigenic dyskinesia. Methods We analyzed clinical manifestations and performed exome sequencing in a cohort of 163 proline-rich transmembrane protein 2-negative probands, followed by filtering data with a paroxysmal movement disorders gene panel. Sanger sequencing, segregation analysis, and phenotypic reevaluation were used to substantiate the findings. Results The clinical characteristics of the enrolled 163 probands were summarized. A total of 39 heterozygous variants were identified, of which 33 were classified as benign, likely benign, and uncertain significance. The remaining 6 variants (3 novel, 3 documented) were pathogenic and likely pathogenic. Of these, 3 were de novo (potassium calcium-activated channel subfamily M alpha 1, c.1534A>G; solute carrier family 2 member 1, c.418G>A; sodium voltage-gated channel alpha subunit 8, c.3640G>A) in 3 sporadic individuals, respectively. The other 3 (paroxysmal nonkinesiogenic dyskinesia protein, c.956dupA; potassium voltage-gated channel subfamily A member 1, c.765C>A; Dishevelled, Egl-10, and Pleckstrin domain containing 5, c.3311C>T) cosegregated in 3 families. All 6 cases presented with typical paroxysmal kinesigenic dyskinesia characteristics, except for the Dishevelled, Egl-10, and Pleckstrin domain containing 5 family, where the proband's mother had abnormal discharges in her temporal lobes in addition to paroxysmal kinesigenic dyskinesia episodes. Conclusions Our findings extend the genotypic spectrum of paroxysmal kinesigenic dyskinesia and establish the associations between paroxysmal kinesigenic dyskinesia and genes classically related to other paroxysmal movement disorders. De novo variants might be a cause of sporadic paroxysmal kinesigenic dyskinesia. © 2018 International Parkinson and Movement Disorder Society.
- Published
- 2018
5. Magnetic Vortex Nanorings: A New Class of Hyperthermia Agent for Highly Efficient In Vivo Regression of Tumors
- Author
-
Yong Yang, Ying Zhang, Jun Ding, Boon-Huat Bay, Haiming Fan, Lingyun Zhao, Cheng Teng Ng, and Xiao-Li Liu
- Subjects
Hyperthermia ,Materials science ,Condensed matter physics ,Cell Survival ,Magnetic Phenomena ,Mechanical Engineering ,Mammary Neoplasms, Experimental ,Hyperthermia, Induced ,medicine.disease ,Ferric Compounds ,Mice ,Nanomedicine ,Nuclear magnetic resonance ,Mechanics of Materials ,In vivo ,MCF-7 Cells ,medicine ,Animals ,Humans ,Nanoparticles ,General Materials Science ,Magnetic vortex - Published
- 2015
6. Synthesis of Dumbbell-Like Gold-Metal Sulfide Core-Shell Nanorods with Largely Enhanced Transverse Plasmon Resonance in Visible Region and Efficiently Improved Photocatalytic Activity
- Author
-
Liang Ma, Qu-Quan Wang, Da-Jie Yang, Xiao-Li Liu, Shan Liang, and Li Zhou
- Subjects
chemistry.chemical_classification ,Materials science ,Sulfide ,Condensed Matter Physics ,Photochemistry ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry.chemical_compound ,chemistry ,Electrochemistry ,Rhodamine B ,Photocatalysis ,Nanorod ,Surface plasmon resonance ,Photodegradation ,Plasmon ,Visible spectrum - Abstract
The metallic nanostructures with unique properties of tunable plasmon resonance and large field enhancement have been cooperated with semiconductor to construct hetero-nanostructures for various applications. Herein, a general and facile approach to synthesize uniform dumbbell-like gold–sulfide core–shell hetero-nanostructures is reported. The transformation from Au nanorods (NRs) to dumbbell-like Au NRs and coating of metal sulfide shells (including Bi2S3, CdS, CuxS, and ZnS) are achieved in a one-pot reaction. Due to the reshaping of Au core and the deposition of sulfide shell, the plasmon resonances of Au NRs are highly enhanced, especially the about 2 times enhancement for the visible transverse plasmon resonance compared with the initial Au NRs. Owing to the highly enhanced visible light absorption and strong local electric field, we find the photocatalytic activity of dumbbell-like Au–Bi2S3 NRs is largely enhanced compared with pure Bi2S3 and normal Au–Bi2S3 NRs by testing the photodegradation rate of Rhodamine B (RhB). Moreover, the second-layer sulfide can be coated and the double-shell Au–Bi2S3–CdS hetero-nanostructures show further improved photodegradation rate, especially about 2 times than that of Degussa P25 TiO2 (P25) ascribing to the optimum band arrangement and then the prolonged lifetime of photo-generated carriers.
- Published
- 2014
7. Orientation Mediated Enhancement on Magnetic Hyperthermia of Fe3O4Nanodisc
- Author
-
Xiao-Li Liu, Jie Fang, Tun Seng Herng, Taishi Zhang, Xianhui Xu, Yunbo Lv, Wen Xiao, Weixing Xia, Jun Ding, and Yong Yang
- Subjects
Materials science ,Nanostructure ,business.industry ,Isotropy ,Specific absorption rate ,Nanoparticle ,Nanotechnology ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Magnetic field ,Biomaterials ,Magnetic hyperthermia ,Quality (physics) ,Electrochemistry ,Optoelectronics ,business ,Nanodisc - Abstract
A two-step chemical approach to synthesize high quality Fe3O4 nanodisc is reported. The magnetic hyperthermia properties of the nanodisc and isotropic nanoparticles are investigated systematically. The results suggest that the nanodisc shows much higher specific absorption rate (SAR) than isotropic nanoparticles. This is attributed to the parallel alignment of nanodisc with respect to the alternating current magnetic field, which is confirmed by good agreement between experimental results and micromagnetic simulation. It is found that such parallel alignment could enhance the SAR value by a factor of approximate to 2 with respect to the randomly oriented case. The above results indicate that the nanodisc provides an excellent thermal seed for magnetic hyperthermia. This study sheds the light on the magnetic hyperthermia mechanism of magnetic nanodisc and it also opens the window to explore high efficiency thermal seeds by controlling the orientation of magnetic nanostructures.
- Published
- 2014
8. Tunable Plasmon Enhancement of Gold/Semiconductor Core/Shell Hetero-Nanorods with Site-Selectively Grown Shell
- Author
-
Da-Jie Yang, Fan Nan, Li Zhou, Qu-Quan Wang, Xiao-Li Liu, Shan Liang, Zhong-Hua Hao, Xue-Feng Yu, and Jiahong Wang
- Subjects
Materials science ,business.industry ,Shell (structure) ,Saturable absorption ,Nanotechnology ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Core (optical fiber) ,Semiconductor ,Optoelectronics ,Nanorod ,Surface plasmon resonance ,business ,Plasmon ,Localized surface plasmon - Abstract
Metal/semiconductor Au/Ag2S core/shell hetero-nanorods with controlled morphology are synthesized and their tunable plasmon enhancements (involving linear and nonlinear optical processes) are demonstrated. The synthesis involves site-selective deposition of Ag layer onto Au nanorod seeds, followed by site-selective sulfuration of the Ag layer, resulting in three types of Au/Ag2S core/shell nanorods with complete, corner-opened, or end-opened shells. The plasmon resonance and local field confinements varing with shell morphology are analyzed using FDTD calculations. Nonlinear measurements reveal that the Ag2S shell with appropriate morphology leads to the nonlinear refraction and saturable intensity of the Au nanorods increasing 7.6 and 4.1 times, respectively, which indicates strong plasmon enhancements and energy relaxation in the Au/Ag2S core/shell nanorods. This site-selective shell growth strategy offers a constructive bottom-up approach to maneuver the optical properties of plasmonic nanocrystals for various applications.
- Published
- 2014
9. Magnetic Nanomaterials for Advanced Regenerative Medicine: The Promise and Challenges
- Author
-
Jin Zhou, Huan Zhang, Shizhu Chen, Xing-Jie Liang, Xiao-Li Liu, and Haiming Fan
- Subjects
Materials science ,Signal Pathways ,Mechanical Engineering ,Regeneration (biology) ,Nanotechnology ,02 engineering and technology ,equipment and supplies ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Regenerative medicine ,0104 chemical sciences ,Nanomaterials ,Mechanics of Materials ,General Materials Science ,0210 nano-technology ,human activities ,Cell aging - Abstract
The recent emergence of numerous nanotechnologies is expected to facilitate the development of regenerative medicine, which is a tissue regeneration technique based on the replacement/repair of diseased tissue or organs to restore the function of lost, damaged, and aging cells in the human body. In particular, the unique magnetic properties and specific dimensions of magnetic nanomaterials make them promising innovative components capable of significantly advancing the field of tissue regeneration. Their potential applications in tissue regeneration are the focus here, beginning with the fundamentals of magnetic nanomaterials. How nanomaterials-both those that are intrinsically magnetic and those that respond to an externally applied magnetic field-can enhance the efficiency of tissue regeneration is also described. Applications including magnetically controlled cargo delivery and release, real-time visualization and tracking of transplanted cells, magnetic regulation of cell proliferation/differentiation, and magnetic activation of targeted ion channels and signal pathways involved in regeneration are highlighted, and comments on the perspectives and challenges in magnetic nanomaterial-based tissue regeneration are given.
- Published
- 2018
10. Differential mitochondrial proteome analysis of human lung adenocarcinoma and normal bronchial epithelium cell lines using quantitative mass spectrometry
- Author
-
Xuede Zhang, Yuping Zhang, Wei Zhang, Biaoxue Rong, Zongjuan Ming, Zequn Niu, Wei Li, Yanli Hou, Shuanying Yang, Xiao-li Liu, and Yanting Li
- Subjects
Pulmonary and Respiratory Medicine ,A549 cell ,Differential centrifugation ,biology ,Cell ,Vimentin ,General Medicine ,Mitochondrion ,medicine.disease ,Molecular biology ,medicine.anatomical_structure ,Oncology ,Proteome ,medicine ,biology.protein ,Adenocarcinoma ,Lung cancer - Abstract
Background Lung cancer is one of the higher incidences of malignant tumors around the world. At present, tumor markers CEA, CA19-9, and CA-125 in serum are used for the diagnosis of lung cancer, however, fewer studies have shown tumor markers for early diagnosis. Therefore, using quantitative mass spectrometry, differential mitochondrial proteome analysis was performed, comparing human lung adenocarcinoma and normal bronchial epithelium cells. Methods A human lung adenocarcinoma cell line A549 and a normal human bronchial epithelial cell line 16HBE were cultured in vitro. The cell mitochondria of the two cell lines were extracted and purified by differential centrifugation and percoll density gradient centrifugation. The integrity and purity of mitochondria were validated by electron microscopy and Western-blot. The proteins/peptides from lung cancer cells and normal cells were marked by the same amount of relative and absolute quantification of ectopic tags (iTRAQ). The mixed samples were analyzed and identified by two-dimensional liquid chromatography – tandem mass spectrometry (2D-LC-MS/MS). The proteome was analyzed with different bioinformatic tools. Results One hundred and sixty-one mitochondrial proteins were identified. One hundred and fifty-three mitochondrial proteins, which were expressed differently between 16HBE cells and A549 cells, were identified. Sixty-seven proteins were high expression, while 86 proteins were lower expression. Expression of three proteins: ornithine aminotransferase (OAT), heat shock protein beta90 (HSP90), and vimentin (VIM), was increased more than twice. Our results, in combination with the literature review, suggest that HSP90 and Vimentin may be the new tumor markers of lung cancer.
- Published
- 2013
11. LC-ESI-MS/MS analysis and pharmacokinetics of jolkinolide B, a potential antitumor active component isolated fromEuphorbia fischeriana, in rat plasma
- Author
-
Yang Li, Sixi Zhang, Xiao-Li Liu, and Zhen-Gang Cai
- Subjects
Pharmacology ,Chromatography ,Lc esi ms ms ,Chemistry ,Electrospray ionization ,Clinical Biochemistry ,Jolkinolide B ,Selected reaction monitoring ,General Medicine ,Plasma ,Biochemistry ,Analytical Chemistry ,Pharmacokinetics ,Euphorbia fischeriana ,Drug Discovery ,Active component ,Molecular Biology - Abstract
A simple, specific and reproducible liquid chromatography–electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was achieved on a Venusil MP-C18 column using an isocratic elution. Jolkinolide B and osthole (internal standard) were monitored by positive electrospray ionization in the selected reaction monitoring mode. Good linearity (r2 > 0.996) was achieved by a weighted (1/x2) linear least-squares regression over a concentration range of 6.50–2600 ng/mL. The accuracy and precision of the assay were satisfactory and the method proved to be applicable to pharmacokinetics following a single intravenous bolus injection of jolkinolide B to rats. Copyright © 2013 John Wiley & Sons, Ltd.
- Published
- 2013
12. ChemInform Abstract: Two New Triterpenoid Saponins from Notoginseng Medicinal Fungal Substance
- Author
-
Xin-Wen Wang, Xiao-Li Liu, Wei Xu, Zhi-Dong Qiu, and Cai-Feng Ding
- Subjects
Terpene ,chemistry.chemical_compound ,Triterpenoid ,Traditional medicine ,Chemistry ,Ginsenoside ,General Medicine - Abstract
Isolation and structure-determination of ginsenoside Rh10 (I) and notoginsenoside ST-6 (II) are presented.
- Published
- 2016
13. Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma
- Author
-
Wen Juan Wang, Zu-De Xu, Kohsuke Sasaki, Jia-Wen Xu, Xiuping Liu, Qing-Quan Li, Xi Xi Cao, Jing Da Xu, Xiao-Li Liu, and Tomoko Furuya
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,Receiver operating characteristic ,business.industry ,Hazard ratio ,Cancer ,Anatomical pathology ,General Medicine ,medicine.disease ,Gastroenterology ,Pathology and Forensic Medicine ,Internal medicine ,medicine ,Immunohistochemistry ,Clinical significance ,business ,Stomach cancer ,Survival analysis - Abstract
Xu J D, Furuya T, Cao X X, Liu X L, Li Q Q, Wang W J, Xu J W, Xu Z D, Sasaki K & Liu X P (2010) Histopathology57, 814–824 Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma Aims: BCL2L10 protein is an apoptosis-related member of the Bcl-2 protein family. The clinical significance of its expression in gastric carcinoma is poorly understood. The aim was to investigate BCL2L10 expression and its clinical and prognostic significance in gastric carcinoma patients. Methods and results: Immunohistochemistry, real–time polymerase chain reaction (PCR) and immunoblotting all revealed extensive loss of BCL2L10 expression in gastric cancer cells. The scaled BCL2L10 expression data was categorized into three groups (groups 0–2) to facilitate statistical analysis. A significant correlation was observed between the lower BCL2L10 expression group and shorter disease-free survival (P = 1.956 × 10−18). Multivariate regression analysis showed that loss of BCL2L10 protein expression [P = 4.883 × 10−8, hazard ratio (HR) = 0.252] is an independent prognostic predictor of gastric carcinoma. The receiver operator characteristic (ROC) curve showed that the area for BCL2L10 protein was 0.817 (P = 8.331 × 10−14), indicating that loss of BCL2L10 protein expression is an excellent prognostic predictor of gastric carcinoma. Conclusions: Loss of BCL2L10 protein expression predicts poor clinical outcome in gastric carcinoma.
- Published
- 2010
14. BCL2L10 protein regulates apoptosis/proliferation through differential pathways in gastric cancer cells
- Author
-
Zi Wen Long, Jia-Wen Xu, Xi Xi Cao, Xiuping Liu, Tomoko Furuya, Jing Da Xu, Qing-Quan Li, Kohsuke Sasaki, Xiao-Li Liu, and Zu-De Xu
- Subjects
Programmed cell death ,Transplantation, Heterologous ,Bisulfite sequencing ,Mice, Nude ,Apoptosis ,Biology ,Pathology and Forensic Medicine ,Mice ,Phosphatidylinositol 3-Kinases ,Stomach Neoplasms ,Tumor Cells, Cultured ,Carcinoma ,medicine ,Animals ,Humans ,RNA, Small Interfering ,Promoter Regions, Genetic ,Cell Proliferation ,Reverse Transcriptase Polymerase Chain Reaction ,Cell growth ,Cancer ,DNA Methylation ,medicine.disease ,Mitochondria ,Proto-Oncogene Proteins c-bcl-2 ,Gene Knockdown Techniques ,DNA methylation ,Cancer cell ,Cancer research ,CpG Islands ,Female ,Proto-Oncogene Proteins c-akt ,Neoplasm Transplantation ,Signal Transduction - Abstract
The reason for and consequences of BCL2L10 down-regulation in gastric carcinoma are poorly understood. Our aim was to investigate the function of the protein BCL2L10 in gastric carcinoma. We investigated BCL2L10 expression using quantitative real-time PCR and immunoblotting. The methylation status of the BCL2L10 gene promoter was examined by bisulphite sequencing in fresh gastric normal and carcinoma tissues. We studied apoptosis and proliferation regulation in gastric cancer cell lines using flow cytometry, fluorescence staining, murine xenografting and immunoblotting. Pathway inhibitors were applied to confirm the major pathways involved in apoptosis or proliferation regulation. We observed significant correlations between lower BCL2L10 expression and CpG island hypermethylation of the BCL2L10 gene promoter in gastric carcinoma, apoptosis induced by over-expressed BCL2L10 through mitochondrial pathways, and proliferation accelerated by BCL2L10 siRNA via the PI3K-Akt signalling pathway in gastric cancer cell lines. The pro-apoptotic effect of BCL2L10 and growth promotion by BCL2L10 siRNA in gastric cancer cells suggest that it may be a tumour suppressor.
- Published
- 2010
15. Expression of b-FGF and endostatin and their clinical significance in human osteosarcoma
- Author
-
Xiao-Li Liu, Yun-Xing Su, Chao-Jian Xu, and Jie-Fu Song
- Subjects
musculoskeletal diseases ,Pathology ,medicine.medical_specialty ,business.industry ,Angiogenesis ,Basic fibroblast growth factor ,CD34 ,macromolecular substances ,medicine.disease ,Metastasis ,Neovascularization ,chemistry.chemical_compound ,chemistry ,cardiovascular system ,Cancer research ,Medicine ,Immunohistochemistry ,Osteosarcoma ,Orthopedics and Sports Medicine ,Surgery ,medicine.symptom ,Endostatin ,business ,neoplasms - Abstract
Objective: To investigate the expression and the clinical significance of basic fibroblast growth factor (b-FGF) and endostatin in osteosarcoma. Methods: From January 2003 to December 2005, expression of b-FGF, endostatin and CD34 were detected in 30 osteosarcoma and 30 osteochondroma tissue specimens by the immunohistochemical Elivision method. All data were post-processed with SPSS 13.0 software and prepared for investigation and analysis of these expressions and the relationships between the parameters. Results: (i) The rates of expression of b-FGF, endostatin and CD34 protein in osteosarcoma were 76.7%, 93.3%, and 96.7%, respectively, and in osteochondroma 43.3%, 40.0% and 16.7%, respectively. Each of the three expressions showed obvious differences between the osteosarcoma and the osteochondroma group. (ii) In the osteosarcoma group, expression of endostatin was positively correlated with that of CD34 (P < 0.05, γs = 0.528), and expression of endostatin in poorly differentiated osteosarcoma was much greater than that in highly differentiated osteosarcoma (P= 0.004). Expression of endostatin correlated with osteosarcoma metastasis (P= 0.036). (iii) There was no correlation between b-FGF and endostatin expression rates (P= 0.182) in the osteosarcoma group. Conclusion: Angiogenesis is the basis of tumor metastasis, as well as being an important factor in tumor growth. Expression of endostatin could be adopted as a parameter for the diagnosis of postoperative metastases and for assessing prognosis, and could act as an adjuvant indicator in the grading of osteosarcoma.
- Published
- 2010
16. RACK1: A superior independent predictor for poor clinical outcome in breast cancer
- Author
-
Jia-Wen Xu, Xiao-Li Liu, Wenjuan Wang, Qing-Quan Li, Xi-Xi Cao, Jing-Da Xu, Zu-De Xu, Xiuping Liu, and Qi Chen
- Subjects
Adult ,Oncology ,Cancer Research ,medicine.medical_specialty ,Pathology ,Receptor, ErbB-2 ,Breast Neoplasms ,Receptors, Cell Surface ,Receptors for Activated C Kinase ,Sensitivity and Specificity ,Immunoenzyme Techniques ,Breast cancer ,GTP-Binding Proteins ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Clinical significance ,Survival rate ,Survival analysis ,Aged ,Neoplasm Staging ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Cancer ,Middle Aged ,medicine.disease ,Neoplasm Proteins ,Survival Rate ,Ki-67 Antigen ,Treatment Outcome ,ROC Curve ,Receptors, Estrogen ,Biomarker (medicine) ,Female ,Breast disease ,Receptors, Progesterone ,Breast carcinoma ,business ,Follow-Up Studies - Abstract
We aimed to investigate the expression of RACK1 in breast cancer, evaluate its role in predicting prognosis and compare with commonly used biomarkers: Ki67, ER, PR and HER-2 for patients with breast cancer. The RACK1 expression and its clinical significance were examined in 160 breast carcinoma patients using immunohistochemistry. Correlations of RACK1 expression with other commonly used biomarkers and survival analyses were assessed. Immunohistochemistry results showed that the number of RACK1 cases scoring 0, 1, and 2 were 66, 54, and 40, respectively. RACK1 staining was strongly related to clinical stage, histological grade, Ki67, ER, PR and HER-2 (all p < 0.05). Consistently, all of the cases exhibiting RACK1 staining score 0 were survivors, whereas the majority (55.0%) of those exhibiting RACK1 staining score 2 were deaths. Kaplan-Meier survival analysis of 160 cases revealed a correlation between higher RACK1 expression levels and shorter overall survival times (p < 0.001). Univariate and multivariate analyses revealed that RACK1, tumor size, lymph node metastasis, and HER-2 were independent prognostic factors (all p < 0.05). Interestingly, receiver operator characteristic (ROC) curves showed that the ROC areas for RACK1, Ki67, ER, PR and HER-2 were 0.833, 0.766, 0.446, 0.387, and 0.689, respectively, and the superiority of RACK1 in sensitivity and specificity as biomarker was demonstrated. To our knowledge, it is the first time to investigate the expression of RACK1, and identified that RACK1 is a superior independent biomarker for diagnosis and prognosis comparing with currently widely used diagnostic index in breast carcinoma.
- Published
- 2009
17. Construction and Properties of Hydrophobic Association Hydrogels with High Mechanical Strength and Reforming Capability
- Author
-
Meng Yang, Fengqi Liu, Xiao-Li Liu, Guohui Zhang, Guoqing Jiang, and Chang Liu
- Subjects
Materials science ,Polymers and Plastics ,General Chemical Engineering ,Organic Chemistry ,Macromonomer ,Micelle ,Dissociation (chemistry) ,Chemical engineering ,Self-healing ,Self-healing hydrogels ,Polymer chemistry ,Materials Chemistry ,Copolymer ,Stress relaxation ,Selectivity - Abstract
Hydrophobic association hydrogels (HA-gels) with almost ideal properties were successfully prepared by micellar copolymerization, and the associated micelles acted as physical cross-linking points in the network of HA-gels. HA-gels exhibit exceptional mechanical properties and transparency. However, the most striking properties are that HA-gels possess the capability of self-healing and remolding, which is mainly due to the dissociation and re-association process of the associated micelles. Dried-gels, which were prepared by stretching HA-gels to a certain elongation for a period of time in the air, can be used as shrinkable or thermal sensitivity materials. HA-gels have a broad selectivity for components, so we have synthesized HA-gels with variously available properties by changing a corresponding component: thermoresponsive HA-gels, nanosphere-composite HA-gels, and fluorescent HA-gels. Therefore, we are sure that HA-gels will be widely used in various fields, such as biology, medication, sensors, optics, and oil exploitation.
- Published
- 2009
18. Characterization of the interactions of the nephrin intracellular domain
- Author
-
Ulf Hellman, Xiao Li Liu, Kunimasa Yan, Karl Tryggvason, Pekka Kilpeläinen, Jorma Wartiovaara, Anders Jönsson, Yi Sun, Ekaterina Morgunova, and Timo Pikkarainen
- Subjects
0303 health sciences ,biology ,urogenital system ,030232 urology & nephrology ,Podocyte foot ,Cell Biology ,urologic and male genital diseases ,Actin cytoskeleton ,Biochemistry ,female genital diseases and pregnancy complications ,Podocyte ,Cell biology ,Nephrin ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,FYN ,IQGAP1 ,medicine ,Slit diaphragm ,Podocin ,biology.protein ,Molecular Biology ,030304 developmental biology - Abstract
Nephrin is a signalling cell-cell adhesion protein of the Ig superfamily and the first identified component of the slit diaphragm that forms the critical and ultimate part of the glomerular ultrafiltration barrier. The extracellular domains of the nephrin molecules form a network of homophilic and heterophilic interactions building the structural scaffold of the slit diaphragm between the podocyte foot processes. The intracellular domain of nephrin is connected indirectly to the actin cytoskeleton, is tyrosine phosphorylated, and mediates signalling from the slit diaphragm into the podocytes. CD2AP, podocin, Fyn kinase, and phosphoinositide 3-kinase are reported intracellular interacting partners of nephrin, although the biological roles of these interactions are unclarified. To characterize the structural properties and protein-protein interactions of the nephrin intracellular domain, we produced a series of recombinant nephrin proteins. These were able to bind all previously identified ligands, although the interaction with CD2AP appeared to be of extremely low stoichiometry. Fyn phosphorylated nephrin proteins efficiently in vitro. This phosphorylation was required for the binding of phosphoinositide 3-kinase, and significantly enhanced binding of Fyn itself. A protein of 190 kDa was found to associate with the immobilized glutathione S-transferase-nephrin. Peptide mass fingerprinting and amino acid sequencing identified this protein as IQGAP1, an effector protein of small GTPases Rac1 and Cdc42 and a putative regulator of cell-cell adherens junctions. IQGAP1 is expressed in podocytes at significant levels, and could be found at the immediate vicinity of the slit diaphragm. However, further studies are needed to confirm the biological significance of this interaction and its occurrence in vivo.
- Published
- 2004
19. Activation of Na,KATPase signaling protects against glomerulo‐tubular disconnection
- Author
-
Ann-Christine Eklöf, Agnes B. Fogo, Anita Aperia, Ulla Holtbäck, Ievgeniia Burlaka, and Xiao Li Liu
- Subjects
Chemistry ,Genetics ,Disconnection ,Molecular Biology ,Biochemistry ,Biotechnology ,Cell biology - Published
- 2012
20. Agrin C20 peptide provides neuroprotection by activating a Na,K‐ATPase/Inositol 1,4,5‐Trisphosphate receptor signaling pathway
- Author
-
Xiao Li Liu, Anita Aperia, Alexander A. Bondar, and Zuzana Spicarova
- Subjects
chemistry.chemical_classification ,Agrin ,Peptide ,Receptor signaling ,Biochemistry ,Neuroprotection ,chemistry.chemical_compound ,chemistry ,Genetics ,Inositol ,Na+/K+-ATPase ,Molecular Biology ,Biotechnology - Published
- 2010
21. Depression mediates the relationship between social capital and health‐related quality of life among Chinese older adults in the context of the COVID‐19 pandemic: A cross‐sectional study
- Author
-
Ping Zhang, Xiao‐Li Liu, Rong‐Mei Zhang, and Ning Xia
- Subjects
COVID‐19 ,depression ,health‐related quality of life ,older adults ,social capital ,Nursing ,RT1-120 - Abstract
Abstract Aim To explore the association between social capital and health‐related quality of life (HRQoL) and to determine whether depression mediates the association among Chinese older adults in the context of the COVID‐19 pandemic. Design A descriptive cross‐sectional research design. Methods The Geriatric Depression Scale‐15, Social Capital Questionnaire and 12‐item Short‐Form Health Survey were used to investigate 1201 older adults selected from Jinan, Shandong Province, China, using a multistage stratified cluster random sampling method. Results Pearson's correlation analysis revealed a significant positive correlation between social capital and HRQoL (r = 0.269, p
- Published
- 2023
- Full Text
- View/download PDF
22. Development and validation of a risk score for chest pain with suspected non‐ST‐segment elevation acute coronary syndrome
- Author
-
Chun‐Peng Ma, Xiao‐Li Liu, Jian‐Shuang Feng, and Xue‐Fei Dong
- Subjects
acute coronary syndrome ,chest pain ,NSTE‐ACS ,risk assessment ,risk factors ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Objective To investigate a new risk score for acute chest pain with suspected non‐ST‐segment elevation acute coronary syndrome (NSTE‐ACS). Methods Patients who suffered from Chest pain and suspected NSTE‐ACS were enrolled as subjects. Predictor variables had been analyzed, and a bootstrap technique was used to evaluate the internal validity of the model, and external validation had been assessed for a prospective cohort study. Results Thousand five hundred and sixty‐eight patients had been included in this study. Six predictor variables were found to be significant and were used to develop the model. The C‐statistic of the model was 0.83, and internal validation revealed the stability of the model and the absence of over‐optimism. Patients were given different triage recommendations, and the risk score was prospectively validated. Conclusions A risk score may be a suitable method for assessing the risk of major adverse cardiac events and aiding patient triage in emergency departments among patients with suspected NSTE‐ACS.
- Published
- 2022
- Full Text
- View/download PDF
23. Exclusive real-time monitoring during recurrent laryngeal nerve dissection in conventional monitored thyroidectomy
- Author
-
Xiao-Li Liu, Che-Wei Wu, Yi-Shen Zhao, Tie Wang, Peng Chen, Jing-Wei Xin, Shi-Jie Li, Da-Qi Zhang, Guang Zhang, Yan-Tao Fu, Li-Na Zhao, Le Zhou, Gianlorenzo Dionigi, Feng-Yu Chiang, and Hui Sun
- Subjects
Intraoperative neurophysiological monitoring ,Recurrent laryngeal nerve ,Thyroidectomy ,Medicine (General) ,R5-920 - Abstract
During conventional intermittent intraoperative neuromonitoring (IONM) in thyroidectomy, recurrent laryngeal nerve (RLN) injury is detected by an electromyographic (EMG) loss of signal (LOS) after the nerve dissection. Exclusive continuous monitoring during the phase of RLN dissection may be helpful in detecting adverse EMG changes earlier. A total of 208 RLNs at risk were enrolled in this study. Standardized IONM procedures were followed. We continuously stimulated the RLN at the lower exposed end with a stimulator to exclusively monitor the real-time quantitative EMG change during RLN dissection. Once the amplitude decreased by more than 50% of the initial signal, the surgical maneuver was paused and the RLN was retested every minute for 10 minutes to determine amplitude recovery before restarting the dissection. The procedure was feasible in all patients. No LOS was encountered in this study. Nineteen RLNs had an amplitude reduction of more than 50%. Eighteen nerves showed gradual amplitude recovery (16 nerves had a traction injury and two nerves had a compression injury). After 10 minutes, the recovery was complete (i.e., >90%) in eight nerves, 70–90% in seven nerves, and 50–70% in three nerves. Among these 18 nerves, only one nerve developed temporary vocal palsy because it was exposed to unavoidable repeated nerve traction after restarting the dissection. Another nerve showed no gradual recovery from thermal injury, and developed temporary vocal palsy. The temporary and permanent palsy rates were 1% and 0%, respectively. During intermittent IONM, exclusive real-time monitoring of the RLN during dissection is an effective procedure to detect an adverse EMG change, and prevent severe RLN injuries that cause LOS.
- Published
- 2016
- Full Text
- View/download PDF
24. Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke
- Author
-
Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, and Ya-guo Li
- Subjects
Pathology ,RB1-214 - Abstract
Clinical and experimental data have demonstrated that inflammation plays fundamental roles in the pathogenesis of ischemic stroke. Interleukin-16 (IL-16) is identified as a proinflammatory cytokine that is a key element in the ischemic cascade after cerebral ischemia. We aimed to examine the relationship between the IL-16 polymorphisms and the risk of ischemic stroke in a Chinese population. A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. We found that the rs11556218TG genotype and G allele of IL-16 were associated with significantly increased risks of ischemic stroke (TG versus TT, adjusted OR = 1.88; 95% CI, 1.15–3.07; G versus T, adjusted OR = 1.54; 95% CI, 1.05–2.27, resp.). However, there were no significant differences in the genotype and allele frequencies of IL-16 rs4778889 T/C and rs4072111 C/T polymorphisms between the two groups, even after stratification analyses by age, gender, and the presence or absence of hypertension, diabetes mellitus, hypercholesterolemia, and hypertriglyceridemia. These findings indicate that the IL-16 polymorphism may be related to the etiology of ischemic stroke in the Chinese population.
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.