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Your search keyword '"Yasui, Hiroe"' showing total 14 results
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14 results on '"Yasui, Hiroe"'

1. Sex pheromone of the fall armyworm, Spodoptera frugiperda : identification of a trace component that enhances attractiveness and specificity

Author

Tabata, Jun, primary, Nakano, Ryo, additional, Yasui, Hiroe, additional, Nakamura, Keisuke, additional, Takehara, Koji, additional, Matsuda, Hiroshi, additional, Ikenoue, Yûki, additional, Kusuhata, Yûsuke, additional, Kinjo, Kunio, additional, Nakama, Kazue, additional, Tsuha, Yuko, additional, and Ishikawa, Yukio, additional

Published
2023
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14. Entomogenous fungus Nomuraea rileyi inhibits host insect molting by C22-oxidizing inactivation of hemolymph ecdysteroids.

Author

Kiuchi M, Yasui H, Hayasaka S, and Kamimura M

Subjects
Animals, Chromatography, High Pressure Liquid methods, Ecdysteroids metabolism, Ecdysteroids pharmacology, Hemolymph chemistry, Host-Parasite Interactions, Hydroxyl Radical chemistry, Larva drug effects, Larva growth & development, Mitosporic Fungi classification, Mitosporic Fungi metabolism, Molting, Nuclear Magnetic Resonance, Biomolecular, Oxidation-Reduction, Bombyx growth & development, Bombyx parasitology, Ecdysteroids antagonists & inhibitors, Mitosporic Fungi physiology
Abstract

The entomogenous fungus Nomuraea rileyi reportedly secretes a proteinaceous substance inhibiting larval molt and metamorphosis in the silkworm Bombyx mori. We studied the possibility that N. rileyi controls B. mori development by inactivating hemolymph molting hormone, ecdysteroids. Incubation of ecdysone (E) and 20-hydroxyecdysone (20E) in fungal-conditioned medium resulted in their rapid modification into products with longer retention times in reverse-phase HPLC. Each modified product from E and 20E was purified by HPLC, and identified by NMR as 22-dehydroecdysone and 22-dehydro-20-hydroxyecdysone. Some other ecdysteroids with a hydroxyl group at position C22 were also modified. Injection of the fungal-conditioned medium into Bombyx mori larvae in the mid-4th instar inhibited larval molt but induced precocious pupal metamorphosis, and its injection into 5th instar larvae just after gut purge blocked pupal metamorphosis. In hemolymph of injected larvae, E and 20E disappeared and, in turn, 22-dehydroecdysone and 22-dehydro-20-hydroxyecdysone accumulated. These results indicate that N. rileyi secretes a specific enzyme that oxidizes the hydroxyl group at position C22 of hemolymph ecdysteroids and prevents molting in B. mori larvae., (Copyright 2003 Wiley-Liss, Inc.)

Published
2003
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