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36 results on '"Yim, Sung"'

2. Metabolic Engineering of Corynebacterium glutamicum for High‐Level Ectoine Production: Design, Combinatorial Assembly, and Implementation of a Transcriptionally Balanced Heterologous Ectoine Pathway

Author

Gießelmann, Gideon, primary, Dietrich, Demian, additional, Jungmann, Lukas, additional, Kohlstedt, Michael, additional, Jeon, Eun J., additional, Yim, Sung S., additional, Sommer, Frederik, additional, Zimmer, David, additional, Mühlhaus, Timo, additional, Schroda, Michael, additional, Jeong, Ki J., additional, Becker, Judith, additional, and Wittmann, Christoph, additional

Published
2019
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17. Application of a rapid and selective method for the simultaneous determination of carebastine and pseudoephedrine in human plasma by liquid chromatography–electrospray mass spectrometry for bioequivalence study in Korean subjects

Author

Lee, Myung‐Jae, primary, Lee, Heon‐Woo, additional, Kang, Jong‐Min, additional, Seo, Ji‐Hyung, additional, Tak, Seong‐Kun, additional, Shim, Wangseob, additional, Yim, Sung‐Vin, additional, Hong, Seung Jae, additional, and Lee, Kyung‐Tae, additional

Published
2010
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24. Microarray analysis of transcription factor gene expression in melatonin-treated human peripheral blood mononuclear cells

Author

Ha, Eunyoung, primary, Han, Earl, additional, Park, Hae Jeong, additional, Kim, Hak-Jae, additional, Hong, Mee Suk, additional, Hong, Seung-Jae, additional, Yoon, Kyung Sik, additional, Kang, Insug, additional, Cho, Yong Hoo, additional, Chung, Joo-Ho, additional, Yim, Sung-Vin, additional, and Baik, Hyung Hwan, additional

Published
2006
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25. Positive relationship between melatonin receptor type 1B polymorphism and rheumatoid factor in rheumatoid arthritis patients in the Korean population

Author

Ha, Eunyoung, primary, Choe, Bong‐Keun, additional, Jung, Kyung Hee, additional, Yoon, Seo Hyun, additional, Park, Hae Jeong, additional, Park, Hun Kuk, additional, Yim, Sung‐Vin, additional, Chung, Joo‐Ho, additional, Bae, Hyun‐Su, additional, Nam, Min, additional, Baik, Hyung Hwan, additional, and Hong, Seung‐Jae, additional

Published
2005
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33. Enhanced secretion of recombinant proteins via signal recognition particle (SRP)-dependent secretion pathway by deletion of rrsE in Escherichia coli.

Author

Lee YJ, Lee R, Lee SH, Yim SS, and Jeong KJ

Subjects
Escherichia coli metabolism, Gene Deletion, Gene Knockdown Techniques methods, Mutation genetics, Recombinant Proteins genetics, Escherichia coli genetics, Genetic Enhancement methods, RNA, Ribosomal, 16S genetics, Recombinant Proteins metabolism, Secretory Pathway genetics, Signal Recognition Particle genetics
Abstract

Although signal recognition particle (SRP)-dependent secretion pathway, which is characterized by co-translational translocation, helps prevent cytoplasmic aggregation of proteins before secretion, its limited capacity for the protein secretion is a major hurdle for utilizing the pathway as an attractive route for secretory production of recombinant proteins. Therefore, we developed an Escherichia coli mutant, whose efficiency of secretion via the SRP pathway was dramatically increased. First, we developed a novel FACS-based screening system by combining a periplasmic display system (PECS) and direct fluorescent labeling with the organoarsenic compound, FlAsH-EDT2 . With this screening system, transposon-insertion library of E. coli was screened, and then we isolated mutants which exhibited higher protein production through the SRP pathway than the parental strain. From the genetic analysis, we found that all isolated mutants had the same mutation-disruption of the 16S rRNA gene (rrsE). The positive effect of rrsE deficiency on protein secretion via the SRP pathway was successfully demonstrated using various model proteins including endogenous SRP-dependent proteins, antibodies, and G protein-coupled receptor. For the large-scale production of IgG and GPCR, we performed fed-batch cultivation with the rrsE-deficient mutant, and very high yields of IgG (0.4 g/L) and GPCR (1.4 g/L) were obtained. Biotechnol. Bioeng. 2016;113: 2453-2461. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)

Published
2016
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34. Development of a new platform for secretory production of recombinant proteins in Corynebacterium glutamicum.

Author

Yim SS, Choi JW, Lee RJ, Lee YJ, Lee SH, Kim SY, and Jeong KJ

Subjects
Bioreactors microbiology, Corynebacterium glutamicum growth & development, Endo-1,4-beta Xylanases genetics, Endo-1,4-beta Xylanases metabolism, Protein Sorting Signals, Single-Domain Antibodies genetics, Single-Domain Antibodies metabolism, alpha-Amylases genetics, alpha-Amylases metabolism, Corynebacterium glutamicum genetics, Corynebacterium glutamicum metabolism, Metabolic Engineering methods, Recombinant Proteins genetics, Recombinant Proteins metabolism
Abstract

Corynebacterium glutamicum, which has been for long an industrial producer of various L-amino acids, nucleic acids, and vitamins, is now also regarded as a potential host for the secretory production of recombinant proteins. To harness its potential as an industrial platform for recombinant protein production, the development of an efficient secretion system is necessary. Particularly, regarding protein production in large-scale bioreactors, it would be appropriate to develop a secretory expression system that is specialized for high cell density cultivation conditions. Here we isolated a new signal peptide that mediates the efficient secretion of recombinant proteins under high cell density cultivation conditions. The secretome of C. glutamicum ATCC 13032 under high cell density cultivation conditions was initially investigated, and one major protein was identified as a hypothetical protein encoded by cg1514. Novel secretory production systems were then developed using the Cg1514 signal peptide and its own promoter. Efficient protein secretion was demonstrated using three protein models: endoxylanase, α-amylase, and camelid antibody fragment (VHH). For large-scale production, fed-batch cultivations were also conducted and high yields were successfully achieved--as high as 1.07 g/L (endoxylanase), 782.6 mg/L (α-amylase), and 1.57 g/L (VHH)--in the extracellular medium. From the culture media, all model proteins could be simply purified by one-step column chromatography with high purities and recovery yields. To the best of our knowledge, this is the first report of the development of an efficient secretory expression system by secretome analysis under high cell density cultivation conditions in C. glutamicum., (© 2015 Wiley Periodicals, Inc.)

Published
2016
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35. Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum.

Author

Yim SS, An SJ, Kang M, Lee J, and Jeong KJ

Subjects
Antibodies analysis, Antibodies genetics, Artificial Gene Fusion, Endo-1,4-beta Xylanases analysis, Endo-1,4-beta Xylanases genetics, Flow Cytometry, Gene Expression Profiling, Green Fluorescent Proteins analysis, Green Fluorescent Proteins genetics, RNA, Messenger analysis, RNA, Messenger genetics, Corynebacterium glutamicum genetics, Gene Expression, Metabolic Engineering methods, Promoter Regions, Genetic
Abstract

Corynebacterium glutamicum is an important industrial organism that is widely used in the production of amino acids, nucleotides and vitamins. To extend its product spectrum and improve productivity, C. glutamicum needs to undergo further engineering, including the development of applicable promoter system. Here, we isolated new promoters from the fully synthetic promoter library consisting of 70-bp random sequences in C. glutamicum. Using green fluorescent protein (GFP) as a reporter, highly fluorescent cells were screened from the library by fluorescent activated cell sorting (FACS). Twenty potential promoters of various strengths were isolated and characterized through extensive analysis of DNA sequences and mRNA transcripts. Among 20 promoters, 6 promoters which have different strengths were selected and their activities were successfully demonstrated using two model proteins (antibody fragment and endoxylanase). Finally, the strongest promoter (P(H36)) was employed for the secretory production of endoxylanase in fed-batch cultivation, achieving production levels of 746 mg/L in culture supernatant. This is the first report of synthetic promoters constructed in C. glutamicum, and our screening strategy together with the use of synthetic promoters of various strengths will contribute to the future engineering of C. glutamicum., (© 2013 Wiley Periodicals, Inc.)

Published
2013
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36. Melatonin suppresses NO-induced apoptosis via induction of Bcl-2 expression in PGT-beta immortalized pineal cells.

Author

Yoo YM, Yim SV, Kim SS, Jang HY, Lea HZ, Hwang GC, Kim JW, Kim SA, Lee HJ, Kim CJ, Chung JH, and Leem KH

Subjects
Animals, Caspase 3, Caspases metabolism, Cell Line, Cytochrome c Group metabolism, DNA Fragmentation drug effects, Mice, Pineal Gland metabolism, Poly(ADP-ribose) Polymerases metabolism, S-Nitroso-N-Acetylpenicillamine pharmacology, Apoptosis drug effects, Melatonin pharmacology, Nitric Oxide Donors pharmacology, Pineal Gland cytology, Pineal Gland drug effects, Proto-Oncogene Proteins c-bcl-2 biosynthesis
Abstract

In the present study, we investigated whether melatonin would prevent nitric oxide (NO)-induced apoptotic death of PGT-beta immortalized pineal cells. To examine the protective effect of melatonin, cytotoxicity assay, DNA fragmentation analysis, caspase-3 activity assay, and Western blotting for caspase-3 and poly(ADP-ribose) polymerase (PARP) were performed. Treatment of cells with S-nitroso-N-acetylpenicillamine (SNAP), an NO donor, was shown to induce apoptotic cell death in a dose-dependent manner, and pretreatment with melatonin (0.1 mm) attenuated the occurrence of NO-induced apoptotic cell death. DNA fragmentation in response to NO was also arrested by melatonin. Caspase-3 activity induced by NO was decreased with melatonin treatment. Furthermore, the active fragments of caspase-3 and PARP were almost completely absent following exposure to melatonin. To elucidate the protective mechanisms of action of melatonin, Western blot analyses for Bcl-2 expression and cytochrome c release were carried out. Pretreatment with melatonin (0.1 mm) induced the expression of Bcl-2 and suppressed the release of cytochrome c into the cytosol, thereby arresting NO-induced apoptotic cell death. These results suggest that the antiapoptotic effect of melatonin is associated with induction of Bcl-2 expression in PGT-beta cells, which in turn blocks caspase-3 activation and inhibits cytochrome c release into the cytosol.

Published
2002
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