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39 results on '"Zhang, Sufang"'

11. Synthesis and bioactivity of ( <scp> 13 Z </scp> , <scp> 15 E </scp> )‐octadecadienal: A sex pheromone component from Micromelalopha siversi Staudinger (Lepidoptera: Notodontidae)

Author

Zhen Zhang, Li Guo, Zhang Sufang, Liu Fu, and Xiangbo Kong

Subjects
0106 biological sciences, biology, Chemistry, Stereochemistry, General Medicine, Notodontidae, biology.organism_classification, 01 natural sciences, Chemical synthesis, Lepidoptera genitalia, 010602 entomology, Insect Science, Sex pheromone, Wittig reaction, Pheromone, PEST analysis, Gas chromatography, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

Background Micromelalopha siversi (Staudinger) (Lepidoptera: Notodontidae) is a defoliator of poplar trees, Populus spp. (Salicaceae). In our previous study, 13,15-octadecadienal has been conformed as a female-produced candidate sex pheromone component of M. siversi, but the Z/E stereochemistry of the 1,3-diene system has not been identified so far. Results Four unsaturated aliphatic aldehydes, Z13,E15-18:Ald, Z13,Z15-18:Ald, E13,E15-18:Ald, and E13,Z15-18:Ald, were synthesized from the commercially available 12-bromo-1-decanol mainly by alkylation of lithium alkyne, normal Wittig or Wittig-Schlosser olefination, and hydroboration-protonolysis. According to gas chromatography (GC) analysis of pheromone gland extracts, Z13,E15-18:Ald was the main component, and a small amount of Z13,Z15-18:Ald was also detected, with a ratio of approximately 7:3. However, the results of GC-electroantennographic detection (GC-EAD) showed that Z13,E15-18:Ald was the only compound with electrophysiological activity, whereas Z13,Z15-18:Ald elicited no activity. In the field, traps baited with only Z13,E15-18:Ald resulted in much superior results to those with Z13,Z15-18:Ald as well as the Z13,E15-18:Ald and Z13,Z15-18:Ald binary mixture. Conclusions Based on geometrically selective synthesis and bioactivity tests, the active sex pheromone component of M. siversi has been identified as Z13,E15-18:Ald, the pheromone component that has not been identified in Lepidoptera before. The synthetic component was attractive to male moths in preliminary field traps, which provides novel technologies to monitor and control this pest.

Published
2020

38. Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation.

Author

Lin X, Gao N, Liu S, Zhang S, Song S, Ji C, Dong X, Su Y, Zhao ZK, and Zhu B

Subjects
Agrobacterium tumefaciens genetics, Chromatography, High Pressure Liquid, Chromosome Mapping, Chromosome Walking, Genotype, Mass Spectrometry, Mutagenesis, Insertional, Phenotype, Carotenoids biosynthesis, Carotenoids chemistry, Metabolic Engineering, Metabolic Networks and Pathways genetics, Rhodotorula genetics, Rhodotorula metabolism, Transformation, Genetic
Abstract

The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciens-mediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright © 2017 John Wiley & Sons, Ltd., (Copyright © 2017 John Wiley & Sons, Ltd.)

Published
2017
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39. Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides.

Author

Wang Y, Lin X, Zhang S, Sun W, Ma S, and Zhao ZK

Subjects
Basidiomycota drug effects, Cinnamates pharmacology, Cloning, Molecular, Hygromycin B analogs & derivatives, Hygromycin B pharmacology, Phenotype, Transformation, Genetic, Basidiomycota genetics, Genetic Engineering, Genetic Vectors genetics, Genome, Fungal genetics, Promoter Regions, Genetic genetics
Abstract

The oleaginous yeast Rhodosporidium toruloides is an unconventional yeast species that can accumulate a high content of lipids. Because it belongs to the basidiomycetous group of fungus, limited tools and functional elements are available for genetic engineering of R. toruloides and related red yeasts. Here we report the functional evaluation of five constitutive promoters from this yeast. We assembled a reporter gene expression cassette, consisting of a promoter, the hygromycin gene (HYG) and the nos terminator, and inserted it into the binary vector pZPK. Hygromycin-resistant transformants were obtained when R. toruloides cells were co-cultured with Agrobacterium tumefaciens AGL1 cells harbouring the engineered vector. Genomic integration of the reporter cassette was verified by successful amplification of target DNA fragments. Quantitative PCR analysis suggested that the transformant had only one copy of the reporter cassette. The strength of these promoters was demonstrated at the phenotypic level on the hygromycin-gradient plate and at the transcriptional level by real-time quantitative PCR. It was found that the strengths of these promoters varied no more than five-fold and followed a decreasing sequence of PPGI, PPGK, PFBA, PTPI, and PGPD. This study established new genetic elements for the construction of superior R. toruloides strains to produce advanced biofuels and related chemicals., (Copyright © 2015 John Wiley & Sons, Ltd.)

Published
2016
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