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Your search keyword '"Zummo, G"' showing total 18 results
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18 results on '"Zummo, G"'

1. Hsp60 secretion and migration from cancer cells: a proposal for a multistage pathway

Author

Alberto Fucarino, Davide Corona, E. Conway de Macario, Anna Maria Merendino, Fabio Bucchieri, Alessandro Pitruzzella, Francesco Cappello, Zummo G, Alberto J.L. Macario, Sabrina David, Felicia Farina, Giosalba Burgio, Claudia Campanella, Campanella, C, Cappello, F, Bucchieri, F, Merendino, AM, Fucarino, A, David, S, Pitruzzella, A, Farina, F, Burgio, G, Corona, D, Conway de Macario, E, Zummo, G, and Macario, AJL

Subjects
Cancer cells Secretion Hsp60, Cancer cell, Genetics, HSP60, Secretion, Biology, Molecular Biology, Biochemistry, Biotechnology, Cell biology
Published
2012

12. OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells

Author

Vito Marcianò, Angela De Luca, Filippo Macaluso, Francesco Cappello, Nella Maria Ardizzone, Antonella Marino Gammazza, Valentina Di Felice, Antonio De Luca, Lucrezia Manente, Giovanni Zummo, DI FELICE, V, Ardizzone, N. M., DE LUCA, X, Marcianò, V, Gammazza, A. M., Macaluso, F, Manente, L, Cappello, F, DE LUCA, Antonio, Zummo, G., Di Felice, V, Ardizzone, NM, De Luca, A, Marino Gammazza, A, and Zummo, G

Subjects
Serum, Scaffold, Physiology, Cellular differentiation, LIM-Homeodomain Proteins, Clinical Biochemistry, Nerve Tissue Proteins, Cell Separation, Biology, Muscle Development, Collagen Type I, Nestin, Rats, Sprague-Dawley, Intermediate Filament Proteins, Microscopy, Electron, Transmission, Troponin T, Animals, Myocyte, Myocytes, Cardiac, Horses, Transcription factor, Homeodomain Proteins, Myosin Heavy Chains, Tissue Scaffolds, Settore BIO/16 - Anatomia Umana, Myocardium, Cell Differentiation, Cell Biology, Anatomy, Phenotype, stem cell, OPLA scaffold, Actins, In vitro, Clone Cells, GATA4 Transcription Factor, Rats, Cell biology, Adult Stem Cells, Proto-Oncogene Proteins c-kit, Connexin 43, Female, Stem cell, Transcription Factors
Abstract

In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold with horse serum to obtain fine myocardial differentiation. C-Kit(POS)/Sca-1(POS) CSCs fully differentiated in vitro when an environment more similar to the CSC niche was created. These experiments demonstrated an important model for the study of the biology of CSCs and the biochemical pathways that lead to myocardial differentiation. The results pave the way for a new surgical approach.

Published
2009

13. Cardiac Stem Cell Research: An Elephant in the Room?

Author

Filippo Macaluso, Angela De Luca, A Montalbano, Nella Maria Ardizzone, Valentina Di Felice, Maria Luisa Colorito, Antonella Marino Gammazza, Francesco Cappello, Giovanni Zummo, Di Felice, V, De Luca, A, Colorito, ML, Montalbano, A, Ardizzone, NM, Macaluso, F, Marino Gammazza, A, Cappello, F, and Zummo, G

Subjects
Pathology, medicine.medical_specialty, Histology, Heart Diseases, medicine.medical_treatment, CD34, heart failure, Stem-cell therapy, Biology, Embryonic stem cell, Cell therapy, Embryo Research, Amniotic epithelial cells, medicine, Humans, cardiac immature cell, cell therapy, Anatomy, Stem cell, Embryonic Stem Cells, Ecology, Evolution, Behavior and Systematics, Stem Cell Transplantation, Biotechnology, Adult stem cell, Stem cell transplantation for articular cartilage repair
Abstract

Heart disease is the leading cause of death in the industrialized world, and stem cell therapy seems to be a promising treatment for injured cardiac tissue. To reach this goal, the scientific community needs to find a good source of stem cells that can be used to obtain new myocardium in a very period range of time. Since there are many ethical and technical problems with using embryonic stem cells as a source of cells with cardiogenic potential, many laboratories have attempted to isolate potential cardiac stem cells from several tissues. The best candidates seem to be cardiac "progenitor" and/or "stem" cells, which can be isolated from subendocardial biopsies from the same patient or from embryonic and/or fetal myocardium. Regardless of the technique used to isolate and characterize these cells, it appears that the different cells isolated from adult myocardium to date are all phenotypic variations of a unique cell type that expresses several markers, such as c-Kit, CD34, MDR-1, Sca-1, CD45, nestin, or Isl-1, in various combinations.

Published
2009

14. A comparative analysis of the products of GROEL-1 gene fromChlamydia trachomatisserovar D and the HSP60 var1 transcript fromHomo sapienssuggests a possible autoimmune response

Author

Claudia Campanella, A. Marino Gammazza, L. Mularoni, V. Di Felice, Francesco Cappello, Giovanni Zummo, Campanella, C, Marino Gammazza, A, Mularoni, L, Cappello, F, Zummo, G, and Di Felice, V

Subjects
Serotype, animal structures, Transcription, Genetic, Molecular Sequence Data, Immunology, Autoimmunity, Chlamydia trachomatis, chemical and pharmacologic phenomena, Biology, medicine.disease_cause, complex mixtures, Epitope, Genetics, medicine, Humans, Amino Acid Sequence, Molecular Biology, Gene, Genetics (clinical), Base Sequence, fungi, Chaperonin 60, General Medicine, Chlamydia Infections, Hsp60, Chlamydia trachomatis, GroEL, Homo sapiens, HSP60, Sequence Alignment
Abstract

Summary Chlamydia trachomatis serovar D produces large quantities of HSP60-1 during infections, which accumulate inside the host cell inducing autoimmunity. We compare the aminoacid sequences of the human HSP60 with the bacterial counterpart to better elucidate how CTHSP60 may simulate HSP60 from human origin during infection and may induce an autoimmune response. As a result of the comparison we suggest several possible epitopes of the CTHSP60, which may induce autoimmunity.

Published
2009

15. Distribution of mitochondrial chaperonins in lung cells

Author

M. Lo Iacono, Simona Corrao, Francesca Magno, G. La Rocca, Rita Anzalone, Francesco Cappello, Felicia Farina, A.J.L. Macario, Tiziana Loria, E. Conway de Macario, Giovanni Zummo, Cappello, F, Corrao, S, Anzalone, R, Magno, F, Loria, T, Lo Iacono, M, Farina, F, Zummo, G, Conway de Macario, E, Macario, AJ, and La Rocca, G

Subjects
Lung, medicine.anatomical_structure, Chaperonins, Hsp10, Hsp60, lung, respiratory diseases, fibroblasts, epithelial cells, Settore BIO/16 - Anatomia Umana, Genetics, medicine, Distribution (pharmacology), Biology, Molecular Biology, Biochemistry, Biotechnology, Chaperonin, Cell biology
Published
2011

17. Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery.

Author

Campanella C, Rappa F, Sciumè C, Marino Gammazza A, Barone R, Bucchieri F, David S, Curcurù G, Caruso Bavisotto C, Pitruzzella A, Geraci G, Modica G, Farina F, Zummo G, Fais S, Conway de Macario E, Macario AJ, and Cappello F

Subjects
Adenocarcinoma metabolism, Adenocarcinoma surgery, Aged, Aged, 80 and over, Biomarkers, Tumor analysis, Blotting, Western, Chaperonin 60 analysis, Colonic Neoplasms metabolism, Colonic Neoplasms surgery, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunohistochemistry, Male, Middle Aged, Mitochondrial Proteins analysis, Real-Time Polymerase Chain Reaction, Adenocarcinoma pathology, Chaperonin 60 metabolism, Colonic Neoplasms pathology, Exosomes metabolism, Mitochondrial Proteins metabolism
Abstract

Background: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer., Methods: The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real-time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis., Results: Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal., Conclusions: The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated., (© 2015 American Cancer Society.)

Published
2015
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18. Hsp60 and Hsp10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease.

Author

Cappello F, Di Stefano A, David S, Rappa F, Anzalone R, La Rocca G, D'Anna SE, Magno F, Donner CF, Balbi B, and Zummo G

Subjects
Adenocarcinoma complications, Adenocarcinoma diagnosis, Adenocarcinoma pathology, Aged, Blotting, Western, Carcinoma, Adenosquamous complications, Carcinoma, Adenosquamous diagnosis, Carcinoma, Adenosquamous pathology, Carcinoma, Bronchogenic complications, Carcinoma, Bronchogenic pathology, Disease Progression, Down-Regulation, Humans, Lung Neoplasms complications, Lung Neoplasms pathology, Middle Aged, Prognosis, Respiratory Mucosa pathology, Carcinoma, Bronchogenic diagnosis, Chaperonin 10 metabolism, Chaperonin 60 metabolism, Lung Neoplasms diagnosis, Pulmonary Disease, Chronic Obstructive complications, Smoking adverse effects
Abstract

Background: The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequency of adenocarcinoma has been reported in patients with COPD. Heat shock proteins (Hsps) are implicated in tumoral cell growth and differentiation. The aim of the present study was to investigate the expression of Hsp60 and Hsp10 in bronchial biopsies from smokers with COPD and in 10 lung cancer patients and to evaluate the association between Hsps expression and carcinogenetic steps of LC., Method: An immunohistochemical study was performed for Hsp60 and Hsp10 in bronchial biopsies from 35 COPD (postbronchodilator forced expiratory volume in 1 second [FEV(1)]: 53 +/- 19% [mean +/- SD]) patients with a history of smoking (53 +/- 34 pack/years) and in 10 patients with adenocarcinoma or adenosquamous carcinoma (ASC). Immunopositivity was quantified in the bronchial epithelium and in specimens with ASC. RESULTS.: In smokers with COPD, 10 out of 35 patients had a normal bronchial epithelium (NBE), 12 showed basal cell hyperplasia (BCH), 5 squamous metaplasia (SM), and 8 dysplasia (Dy). It was found that 58 +/- 23% and 54 +/- 23% of NBE and 48 +/- 29% and 52 +/- 26% of BCH expressed Hsp60 and Hsp10, respectively; in contrast, only 3 +/- 3% and 3.6 +/- 2% of SM, 1.9 +/- 4% and 1.1 +/- 2% of Dy expressed Hsp60 and Hsp10, respectively. ASC specimens were negative for Hsps proteins. Interestingly, NBE also present at the edges of ASC specimens was negative for Hsps proteins., Conclusions: The loss of Hsp60 and Hsp10 immunopositivity is related to the development and progression of bronchial cancer in smokers with COPD.

Published
2006
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