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Your search keyword '"Roberta Gambaretto"' showing total 2 results
Start Over Author "Roberta Gambaretto" Publisher wiley periodicals, inc.
2 results on '"Roberta Gambaretto"'

1. Covalent surface modification of titanium oxide with different adhesive peptides: surface characterization and osteoblast-like cell adhesion

Author

Anna Michela Menti, Antonino Licciardello, Claudio Grandi, Giovanna Iucci, Carlo Di Bello, Giovanni Polzonetti, Maria Teresa Conconi, Andrea Bagno, Nunzio Tuccitto, Roberta Gambaretto, Monica Dettin, Dettin, M, Bagno, A, Gambaretto, R, Iucci, Giovanna, Conconi, Mt, Tuccitto, M, Menti, Am, Grandi, C, DI BELLO, C, Licciardello, A, and Polzonetti, G.

Subjects
biomimetic surface, Materials science, Surface Properties, Molecular Sequence Data, Biomedical Engineering, chemistry.chemical_element, Spectrometry, Mass, Secondary Ion, Nanotechnology, Biomaterials, Rats, Sprague-Dawley, Coated Materials, Biocompatible, Materials Testing, Surface roughness, Animals, Humans, Amino Acid Sequence, Cell adhesion, Cells, Cultured, Titanium, Osteoblasts, Molecular Structure, biomaterial, peptide, cell adhesion, surface characterization, technology, industry, and agriculture, Metals and Alloys, Titanium alloy, Biomaterial, Endothelial Cells, Adhesion, Silanes, Titanium oxide, Rats, chemistry, Ceramics and Composites, Surface modification, Female, Peptides
Abstract

A fundamental goal in the field of implantology is the design of innovative devices suitable foil promoting, implant-to-tissue integration. This result can be achieved by means of surface modifications aimed at optimizing tissue regeneration. In the framework of oral and orthopedic implantology, surface modifications concern both the optimization of titanium/titanium alloy surface roughness and the attachment of biochemical factors able to guide cellular adhesion and/or growth. This article focuses on the covalent attachment of two different adhesive peptides to rough titanium disks. The capability of biomimetic surfaces to increase osteoblast adhesion and the specificity of their biological activity due to the presence of cell adhesion signal-motif have also been investigated. In addition, surface analyses by profilometry, X-ray photoelectron spectroscopy, and time of flight-secondary ion mass spectrometry have been carried out to investigate the effects and modifications induced by grafting procedures.

Published
2009

2. Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation

Author

Francesca Ghezzo, Andrea Bagno, Carlo Di Bello, Roberta Gambaretto, Lucy Di Silvio, Chiara Battocchio, Giovanni Polzonetti, Monica Dettin, Giovanna Iucci, Thushari Herath, Dettin, M, Herath, T, Gambaretto, R, Iucci, G, Battocchio, Chiara, Bagno, A, Ghezzo, F, DI BELLO, C, Polzonetti, Giovanni, DI SILVIO, L., Iucci, Giovanna, Polzonetti, G, M., Dettin, T., Herath, R., Gambaretto, G., Iucci, A., Bagno, F., Ghezzo, C., DI BELLO, G., Polzonetti, and L., DI SILVIO

Subjects
biomimetic surface, Materials science, Surface Properties, Biomedical Engineering, chemistry.chemical_element, Peptide, titanium oxide, Biomaterials, chemistry.chemical_compound, Coated Materials, Biocompatible, X-ray photoelectron spectroscopy, Trifluoroacetic acid, Cell adhesion, peptide, XPS, Humans, Organic chemistry, Peptide sequence, Cells, Cultured, Cell Proliferation, Titanium, chemistry.chemical_classification, Osteoblasts, Metals and Alloys, cell adhesion, Cell Differentiation, Combinatorial chemistry, Titanium oxide, chemistry, Covalent bond, Ceramics and Composites, Peptides
Abstract

Bioactive molecules have been proposed to promote beneficial interactions at bone-implant interfaces for enhancing integration. The main objective of this study was to develop novel methods to functionalize oxidized titanium surfaces by the covalent immobilization of bioactive peptides, through selective reaction involving single functional groups. In the first protocol, an aminoalkylsilane was covalently linked to the Ti oxide layer, followed by covalent binding of glutaric anhydride to the free NH(2) groups. The carboxylic group Of glutaric anhydride was used to condense the free N-terminal group of the side-chain protected peptide sequence. Finally, the Surface was treated with trifluoroacetic acid to deprotect side-chain groups. In the second protocol, the peptide was directly anchored to the Ti oxide surface via UV activation of an arylazide peptide analogue. X-ray photoelectron spectroscopy analyses confirmed that modifications induced onto surface composition were in agreement with the reactions performed. The peptide density of each biomimetic Surface was determined on the basis of radiolabeling and XPS derived reaction yields. The in vitro cellular response of the biomimetic surfaces was evaluated using a primary human osteoblast cell model. Cell adhesion, proliferation, differentiation, and mineralization were examined at initial-, short-, and long-time periods. In was shown that the biomimetic surface obtained through photoprobe-marked analogue that combines an easily-performed modification provides a favorable surface for an enhanced cellular response.

Published
2009

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