1. A substitution mutation in <italic>OsPELOTA</italic> confers bacterial blight resistance by activating the salicylic acid pathway.
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Zhang, Xiao‐Bo, Feng, Bao‐Hua, Wang, Hui‐Mei, Xu, Xia, Shi, Yong‐Feng, He, Yan, Chen, Zheng, Sathe, Atul Prakash, Shi, Lei, and Wu, Jian‐Li
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PLANT plasma membranes , *GENE expression in plants , *RNA sequencing , *CELL death , *PHENYLALANINE , *PLANTS - Abstract
Abstract: We previously reported a spotted‐leaf mutant
pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races ofXanthomonas oryzae pv.oryzae . Here, we report the map‐based cloning of the causal geneOsPELOTA (originally termedsplHM47 ). We identified a single base substitution from T to A at position 556 in the coding sequence ofOsPELOTA , effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over‐expression could rescue the spotted‐leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity toDrosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane.OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in thepelota mutant. RNA‐seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway. [ABSTRACT FROM AUTHOR]- Published
- 2018
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