1. Pseudoginsenoside-F11 attenuates cerebral ischemic injury by alleviating autophagic/lysosomal defects.
- Author
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Liu YY, Zhang TY, Xue X, Liu DM, Zhang HT, Yuan LL, Liu YL, Yang HL, Sun SB, Zhang C, Xu HS, Wu CF, and Yang JY
- Subjects
- Animals, Apoptosis drug effects, Apoptosis physiology, Autophagy physiology, Brain pathology, Brain physiopathology, Brain Edema drug therapy, Brain Edema pathology, Brain Edema physiopathology, Brain Ischemia pathology, Brain Ischemia physiopathology, Chloroquine pharmacology, Disease Models, Animal, Lysosomes drug effects, Lysosomes pathology, Lysosomes physiology, Male, Neuroglia drug effects, Neuroglia pathology, Neuroglia physiology, Neurons drug effects, Neurons pathology, Neurons physiology, Rats, Sprague-Dawley, Stroke pathology, Stroke physiopathology, Autophagy drug effects, Brain drug effects, Brain Ischemia drug therapy, Ginsenosides pharmacology, Neuroprotective Agents pharmacology, Stroke drug therapy
- Abstract
Aims: Pseudoginsenoside-F11 (PF11), an ocotillol-type ginsenoside, has been reported to exert wide-ranging neuroprotective properties. The aim of this study was to investigate the effect and potential mechanisms of PF11 on the autophagic/lysosomal pathway following ischemic stroke., Methods: Male Sprague-Dawley rats underwent permanent middle cerebral artery occlusion (pMCAO). Cerebral ischemia outcome, TUNEL staining, Fluoro-Jade B staining were carried out 24 hours poststroke. The autophagic/lysosomal-related proteins were measured., Results: A single administration of PF11 significantly decreased the infarct area, reduced the brain water content, and improved neurological functions, even 4 hours after the onset of pMCAO. Meanwhile, PF11 lessened the ischemic insult-mediated loss of neurons and activation of astrocytes and microglia. Furthermore, PF11 attenuated pMCAO-induced accumulations of autophagosomes and apoptosis. We further observed a remarkable effect of PF11 in reversing the ischemic insult-induced accumulation of autophagosomes (LC3-II) and abnormal aggregation of autophagic proteins (SQSTM1 and ubiquitin). Furthermore, PF11 was capable of improving lysosomal function and lysosome/autophagosome fusion following pMCAO, and this change was reversed by the lysosomal inhibitor chloroquine. Also, the improvement of ischemic outcome and the antiapoptotic effect induced by PF11 was reversed by CQ., Conclusion: These findings indicate that the autophagic flux is impaired in a rat model of pMCAO, and that PF11 exerts an excellent protective effect against ischemic stroke by alleviating autophagic/lysosomal defects., (© 2017 John Wiley & Sons Ltd.)
- Published
- 2017
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