Search

The recognition of protospacer adjacent motif (PAM) is a key factor for the CRISPR (i.e. clustered regularly interspaced short palindromic repeats)/CRISPR‐associated 9 (Cas9) system to distinguish foreign DNAs from the host genome, and also significantly restricts the targeting scope of the system during genome‐editing applications. Structurally, the PAM interacting (PI) domain, which usually is located in the C‐terminus of Cas9 proteins, directly binds to PAM and plays a key role in determining the recognition specificity. However, several lines of evidence showed that other regions of Cas9 protein beyond the PI domain might also play roles in PAM interaction. Here, we constructed a mosaic SpCas9 protein (xCas9‐NG) by fusing the PI domain of SpCas9 PAM variant, Cas9‐NG with the non‐PI fragment of xCas9 protein that contains multiple amino acid substitutions. We found that non‐PI fragment of xCas9 expanded PAM recognition of the Cas9‐NG PI domain. In addition, xCas9‐NG showed an improved editing efficiency in the majority of targets harboring xCas9 and Cas9‐NG PAMs. Importantly, this finding was also successfully extended to other Cas9 variants, including SpRY and the non‐G SpCas9 series. Together, our work expands the target scope of SpCas9 editing system and demonstrates the notion that the non‐PI domain fragment plays an important role in PAM restriction. [ABSTRACT FROM AUTHOR]