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Mitochondria play a crucial role in numerous biological processes; however, limited methods and research have focused on revealing mitochondrial heterogeneity at the single‐cell level. In this study, we optimized the DNBelab C4 single‐cell ATAC (assay for transposase‐accessible chromatin) sequencing workflow for single‐cell mitochondrial sequencing (C4_mtscATAC‐seq). We validated the effectiveness of our C4_mtscATAC‐seq protocol by sequencing the HEK‐293T cell line with two biological replicates, successfully capturing both mitochondrial content (~68% of total sequencing data) and open chromatin status simultaneously. Subsequently, we applied C4_mtscATAC‐seq to investigate two mouse tissues, spleen and bone marrow, obtained from two mice aged 2 months and two mice aged 23 months. Our findings revealed higher mitochondrial DNA (mtDNA) content in young tissues compared to more variable mitochondrial content in aged tissues, consistent with higher activity scores of nuclear genes associated with mitochondrial replication and transcription in young tissues. We detected a total of 22, 15, and 21 mtDNA mutations in the young spleen, aged spleen, and bone marrow, respectively, with most variant allele frequencies (VAF) below 1%. Moreover, we observed a higher number of mtDNA mutations with higher VAF in aged tissues compared to young tissues. Importantly, we identified three mtDNA variations (m.9821A>T, m.15219T>C, and m.15984C>T) with the highest VAF in both aged spleen and aged bone marrow. By comparing cells with and without these mtDNA variations, we analyzed differential open chromatin status to identify potential genes associated with these mtDNA variations, including transcription factors such as KLF15 and NRF1. Our study presents an alternative single‐cell mitochondrial sequencing method and provides crude insights into age‐related single‐cell mitochondrial variations. [ABSTRACT FROM AUTHOR]

The corrosion behaviors of Ti3SiC2/Cu composites synthesized by spark plasma sintering technique were investigated at 900°C in three molten salts including NaCl, Na2SO4, and Na2CO3, and the related corrosion mechanism was discussed. The morphology and composition of the composites after corrosion were characterized by scanning electron microscopy and X‐ray photoelectron spectroscopy. The results indicated that the weight gain per unit surface area of the composites was negative in molten Na2CO3, while it was positive and relatively small in molten NaCl and Na2SO4. The high weight loss of the composites in molten Na2CO3 was owing to that in molten Na2CO3, an oxidation layer of the composites was dissolved, and oxygen diffused through the layer to corrode the substrate inside. Thus, in the molten Na2CO3, the composites exhibited a worse corrosion resistance. However, in molten NaCl and Na2SO4, an oxidation layer containing SiO2 and TiO2 was formed on the surface of the composites, inhibiting its further corrosion. Conclusively, the composites showed better corrosion resistance in molten NaCl and Na2SO4. [ABSTRACT FROM AUTHOR]

A non‐enzyme photoelectrochemical (PEC) glucose sensor based on α‐Fe2O3 film is investigated. The α‐Fe2O3 film was fabricated via a simple spin coating method. The proposed glucose sensor exhibits good selectivity, a fast response time of <5 s, a linear range of 0.05 to 6.0 mM, sensitivity of 17.23 μA mM−1 cm−2 and a detection limit of 0.05 μM. Meanwhile, the excellent performances of the α‐Fe2O3 sensor were obtained in reproducibility and the long‐term stability under ambient condition. The linear amperometric response of the sensor covers the glucose levels in physiological and clinical for diabetic patients. Therefore, this non‐enzyme PEC sensor based on α‐Fe2O3 film has a great potential application in the development of glucose sensors. [ABSTRACT FROM AUTHOR]

To elucidate the distribution and dispersion of RuO2 species on the reactivity, RuO2−SnO2 catalysts with 2 % and 5 % Ru contents have been prepared with impregnation (IMP), deposition‐precipitation (DP) and co‐precipitation (CP) methods, and probed by CO oxidation. With IMP and DP methods, RuO2 crystallites are predominantly formed on the catalyst surface, which is favorable for CO oxidation. Moreover, the IMP catalyst possesses surface RuO2 having smaller mean crystallite size and better dispersion than the DP catalyst, thus SnRuO‐IMP displays higher activity than SnRuO‐DP at the same Ru loadings. However, with CP method, RuO2 species is mainly present as Ru4+ cations in the lattice of rutile SnO2 to form a solid solution structure below the lattice capacity, which is less reactive than the surface RuO2 due to the restriction by the SnO2 lattice. In conclusion, surface RuO2 is revealed to be the active species, whose amount and dispersion determine the activity of the catalysts. By changing preparation methods, the distribution of RuO2 species in the catalysts is varied and impacts the reactivity of the catalysts evidently. The traditional impregnation is found to be the best method to prepare RuO2−SnO2, which shows the highest activity among all the catalysts. [ABSTRACT FROM AUTHOR]

The electrochemical corrosion behaviors of Ti3SiC2/Cu composite and polycrystalline Ti3SiC2 in a 3.5% NaCl medium were investigated by dynamic potential polarization, potentiostat polarization, and electrochemical impedance spectroscopy. The polycrystalline Ti3SiC2 was tested on the identical condition as a control. The characterizations of XRD, X‐ray photoelectron spectroscopy, scanning electron microscope, and energy‐dispersive spectrometer were used to study the relevant passivation behavior and corrosive mechanism. The self‐corrosion current density of Ti3SiC2/Cu (6.46 × 10−6 A/cm2) was slightly higher than that of Ti3SiC2 (1.64 × 10−7 A/cm2). Under open circuit potential, the corrosion resistance of Ti3SiC2/Cu was better than that of Ti3SiC2. Ti3SiC2/Cu exhibited a typical passivation feature with a narrow passivation interval and a breakdown phenomenon. The better corrosion resistance of Ti3SiC2 was due to the more stable Si layer of the former. In comparison, for Ti3SiC2/Cu composites, Cu reacted with the reactive Si layers in Ti3SiC2 to form Cu–Si compounds and TiC, destroying the weak interaction between Si layers and Ti–C layers. In the other hand, the as‐formed Cu–Si compounds and TiC dissolved during the corrosion of Ti3SiC2/Cu in the 3.5% NaCl medium, causing to the destruction of the passivation film on its surface. [ABSTRACT FROM AUTHOR]

Objective: Young adult patients with cancer are a growing concern. By means of network analysis, this study aimed to explore the interplay between dignity-related distress and quality of life (QoL) in young adult patients with cancer when they undergo active treatments.Methods: In this cross-sectional study, 309 young adults aged 18-39 and diagnosed with malignant tumors were recruited from an oncology center in China between September 2020 and August 2021. Participants completed the Patient Dignity Inventory and SF-36 questionnaires. Network analysis was applied to examine the network structure.Results: Overall, the core facets of dignity-related distress were negatively related to QoL and its corresponding domains, either directly or indirectly. Developmental distress played a central role among estimated networks and strongly interplayed with most QoL domains, especially the mental domains. Symptom distress was the only facet consistently interplayed with the physical domains of QoL (i.e., physical function and bodily pain). The social aspects were also revealed in the association between limited social support and vitality.Conclusions: Early attention must be paid to guarantee the need of preserving dignity and enhancing QoL for young adult patients. [ABSTRACT FROM AUTHOR]