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Ureolytic microbes are abundant in broiler litter (BL), and previous research indicates that microbially induced calcium carbonate precipitation via urea hydrolysis occurs in gypsum‐amended litters. Given that calcium carbonate (CaCO3) is used to correct soil acidity, we hypothesized that the soil‐liming potential of BL + gypsum is greater than that of unamended litter. First, an experiment was conducted to isolate ureolytic bacteria from BL and confirm their ability to precipitate CaCO3. To determine the effects of calcium on urea hydrolysis, six isolates were identified from screenings of 30 colonies collected from different BL sources. Calcium increased the ureolytic rate by 29%–160% and the precipitation of CaCO3 by ~1500% in solution. The 16S rRNA of the isolate with the greatest rate of urea hydrolysis was sequenced and belong to Lysinibacillus macroides. Broiler litter amended with gypsum was inoculated with L. macroides and found to precipitate significantly more CaCO3 than inoculated litter without gypsum. Finally, over the course of a 28‐day incubation, the pH and inorganic‐N of soil, soil + BL, and soil + BL + gypsum were measured. Surprisingly, soil + BL + gypsum was found to have a significantly lower pH than soil + BL for the first 21 days. We propose that the decreased liming ability of BL + gypsum is due to a combination of pH buffering mechanisms and increased nitrification. Although gypsum is a useful litter amendment, it cannot be used to increase the liming ability of litter, despite the fact that gypsum‐amended litter has more CaCO3. Core Ideas: Calcium ions increase the rate of urea hydrolysis by poultry litter‐derived ureolytic isolates.Inoculating gypsum‐amended litter with ureolytic bacteria increases the CaCO3 and NH4+ content of litter.The soil liming potential of gypsum‐amended litter is not greater than the liming potential of unamended litter, even though treated litter contains more CaCO3. [ABSTRACT FROM AUTHOR]

BACKGROUND: The pH adjustment of acidic red soils with lime materials is beneficial for the reduction of phosphorus (P) fixation. However, the reasons for varying levels of P activation after adding different lime materials have not been fully investigated. Therefore, this study examined changes in soil labile P and P forms after phosphate application to calcium carbonate (CaCO3) and dolomite amended red soil during a 120‐day incubation period. Also change of P sorption properties in the amended soil samples from day 120 were examined through a sorption–desorption experiment. RESULTS: The increase of soil H2O‐P and NaHCO3‐P in the CaCO3 and dolomite amended soil treatments was mainly ascribed to the decline of the NaOH‐P. However, when compared with the control treatment after 120 days, soil Olsen‐P significantly increased by 34% and 66% in the CaCO3 and dolomite treatments. The Hedley P fractionation results demonstrated that the CaCO3 application caused a notable increase of HCl‐P (stable Ca–P), which was 88.4% higher than that in the dolomite treatment. However, the formation of stable P was strongly suppressed in the dolomite treatment due to the presence of magnesium (Mg), which was identified by the negative relationship between M3‐Mg and HCl‐P. In line with these findings, P sorption–desorption work showed weaker P binding energy in the dolomite treatment relative to the CaCO3 treatment. CONCLUSION: In terms of increasing P availability in red soil, this study suggests that dolomite should be used to substitute CaCO3 in order to reduce the soil P fixation. © 2021 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Odonate assemblages were compared between replicate sets of shallow lakes that had been created and acidified by open-cast mining across a large area (2,451 ha) of southwest France (Arjuzanx, Landes); one set of lakes ( n = 5) was experimentally restored by liming with calcium carbonate, whereas another group ( n = 5) was left as untreated reference lakes. Both odonate adults and exuviae were sampled bimonthly during May–August 1998. Elevated turbidity and conductivity in limed lakes were the only physicochemical measures differing between restored and reference lakes, because deacidification occurred naturally, even in reference lakes during the 17 years after the onset of restoration. Restoration by liming can apparently lead to effects on lake turbidity that might be considered adverse. Twenty-four and 19 odonate species occurred among adults and exuviae, respectively, but there were no significant differences in richness between restored and reference sites. However, significantly, more exuviae were collected from the reference sites (588 vs. 180), where exuvial diversity and rank abundance indicated more evenly structured assemblages than those in restored lakes. Ordination showed that adult assemblages differed significantly between restored and reference lakes, and varied highly significantly with lake turbidity. This effect occurred because a small group of generally scarce adults were characteristic of reference sites ( Chalcolestes viridis, Lestes virens, Cordulia aenae, Leucorrhinia albifrons, and Sympetrum sanguineum). Exuviae of these same species were less abundant at restored sites, but exuvial assemblages overall did not discriminate between restored and reference lakes. We conclude that lake restoration by liming can reduce diversity and larval numbers among odonates and subtly affects adult assemblages. In this case study, adult assemblages discriminated best between the lake types involved in the experiment, but important additional information arose from exuvial abundance and structure. This study indicates that natural recovery processes after acidification in formerly open-cast areas––rather than chemical intervention through liming––might lead to preferable conservation outcomes. [ABSTRACT FROM AUTHOR]