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Two different types of grinding stones, grinding slabs and rollers, as well as mortars and pestles from the Shuangdun site (ca. 7.3–6.8 ka BP) in the middle Huai River region, were subjected to starch grain analysis to investigate the evolution of plant food processing tools during the transition period from a gathering–fishing–hunting economy to farming. The results suggest that both types of grinding stones were used to process various plant species, including Triticeae, Job's tears (Coix lacryma‐jobi), lotus root (Nelumbo nucifera), and bean (Vigna spp.). However, starch grains of rice (Oryza sativa) were only discovered from mortar and pestle tools, indicating that mortars and pestles might have become a fixed combination tool for rice processing. Damage characteristics analysis for different kinds of starches indicated that mortars and pestles were also used for grinding plant foods besides dehusking rice, whereas some roughly made slabs no longer had a grinding function and were used only for pounding some specific plant foods. The functional degeneration of grinding slabs coincides with the rise of farming in Neolithic China around 7,000 BP. This study provides new evidence for understanding the evolution of prehistoric human plant food processing tools in Neolithic Eastern China. [ABSTRACT FROM AUTHOR]

Anthracnose caused by Colletotrichum species is a serious disease on a range of economically important hosts. To determine the Colletotrichum species in coffee plantations in Hainan, China, 55 isolates were obtained from Coffea arabica (arabica) and C. canephora var. robusta (robusta) in five counties. Initially, partial sequences of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) were used to measure fungal genetic diversity. Then a subset of 23 isolates was selected to represent the range of genetic diversity, varieties and geographic origin for further multilocus phylogenetic analyses. These isolates belong to eight known Colletotrichum species from three Colletotrichum species complexes, including gloeosporioides (C. endophytica, C. fructicola, C. ledongense, C. siamense and C. tropicale), boninense (C. karstii), gigasporum (C. gigasporum), and one singleton species (C. brevisporum). Of these, C. siamense was isolated in all sampled counties and C. fructicola was identified in three counties. The other six species were isolated only in one or two counties. Only C. siamense and C. fructicola were isolated from arabica, whereas all eight species were isolated from robusta. Occurrence of C. brevisporum, C. endophytica, C. ledongense and C. tropicale in coffee has not been reported previously. Pathogenicity tests showed that all eight species were pathogenic to coffee leaves and fruit. In vitro tests showed that Colletotrichum isolates from coffee in Hainan were most sensitive to prochloraz, less sensitive to carbendazim, propiconazole and difenoconazole, and least sensitive to myclobutanil. [ABSTRACT FROM AUTHOR]