1. Lysophosphatidylcholine facilitates the pathogenesis of psoriasis through activating keratinocytes and T cells differentiation via glycolysis.
- Author
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Liu P, Zhou Y, Chen C, Yan B, Li L, Zhu W, Li J, Chen M, Su J, Kuang Y, Chen X, and Peng C
- Subjects
- Mice, Animals, Lysophosphatidylcholines adverse effects, Lysophosphatidylcholines metabolism, Keratinocytes metabolism, Imiquimod adverse effects, Cell Differentiation, Disease Models, Animal, Mice, Inbred BALB C, Skin pathology, Psoriasis pathology, Skin Diseases pathology
- Abstract
Background: Although abnormal metabolism plays a critical role in the pathogenesis of psoriasis, the details are unclear., Objectives: Here, we identified to explore the role and mechanism of lysophosphatidylcholine (LPC) on the pathogenesis of psoriasis., Methods: The level of LPC in plasma and skin lesions and the expression of G2A on skin lesions of psoriasis patients were detected by enzyme-linked immunosorbent assay, liquid chromatography-tandem mass spectrometry, or immunohistochemistry, respectively. The glycolysis in the skin lesions of imiquimod (IMQ)-induced psoriasis-like mouse model was detected by extracellular acidification rate. LPC was subcutaneously injected into IMQ-treated mouse ears, and the phenotype as well as the glycolysis were evaluated. Exploring the effects and mechanism of LPC on keratinocytes and CD4
+ T cells by culturing primary keratinocytes and CD4+ T in vitro., Results: We found that LPC was significantly increased both in the plasma and skin lesions of psoriatic patients, while G2A, exerting an essential role in LPC-inducing biological functions, was increased in psoriatic lesions. The abundance of LPC was positively correlated with glycolytic activity in the psoriasis-like mouse model. LPC treatment facilitated psoriasis-like inflammation and glycolytic activity in skin lesions. Mechanistically, the LPC/G2A axis significantly triggered glycolytic activity and produced inflammatory factors in keratinocytes, and blockade of glycolysis abrogated LPC-induced expression of inflammatory mediators in keratinocytes. LPC activated STAT1, resulting in recognition and binding to the promoters of GCK and PKLR, which are glycolytic rate-limiting enzymes. Furthermore, the LPC/G2A axis directly benefited Th1 differentiation, which was dependent on LPC-induced glycolytic activity. Notably, LPC indirectly facilitated Th17 differentiation by inducing the secretion of IL-1β in keratinocytes-T cells coculture system., Conclusions: Taken together, our findings revealed the role of the LPC/G2A axis in the pathogenesis of psoriasis; targeting LPC/G2A is a potential strategy for psoriasis therapy., (© 2023 European Academy of Dermatology and Venereology.)- Published
- 2023
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