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A role of IL‐10 is down‐regulating T‐cell responses to schistosome antigens. Since SmATPDases can be correlated to modulation of the immune response, we evaluated the expression of enzymes in S. mansoni eggs. Faecal samples were collected from 40 infected individuals to detect coding regions of the SmATPDases. The cytokines were measured in supernatants of PBMC. The analysis was performed by the global median determination and set up high producers (HP) of cytokines. Six individuals expressed SmATPDase1, six expressed SmATPDase2 and six expressed both enzymes. The group who expressed only SmATPDase1 showed a high frequency of IFN‐γ, TNF IL‐4 HP; individuals who expressed only SmATPDase2 showed a high frequency of IFN‐γ, IL‐6 and IL‐4 HP; and individuals who expressed both enzymes showed a high frequency of IL‐10 HP. The comparison of the IFN‐γ/IL‐10 ratio presented higher indices in the group who had SmATPDase 2 expression than those who had the expression of both enzymes. The positive correlation between infection intensity and IL‐10 levels remained only in the positive SmATPDase group. The IL‐10 is the only cytokine induced by the expression of both enzymes. Our data suggest that the expression of both enzymes seems to be a factor that modulates the host immune response by inducing high IL‐10 production. [ABSTRACT FROM AUTHOR]

Cutinase from Thermobifida fusca was used to esterify the hydroxyl groups of cellulose with the fatty acids from triolein. Cutinase and triolein were pre-adsorbed on cotton and the reaction proceeded in a dry state during 48 h at 35°C. The cutinase-catalyzed esterification of the surface of cotton fabric resulted in the linkage of the oleate groups to the glycoside units of cotton cellulose. The superficial modification was confirmed by performing ATR-FTIR on treated cotton samples and by MALDI-TOF analysis of the liquors from the treatment of the esterified cotton with a crude cellulase mixture. Modified cotton fabric also showed a significant increase of hydrophobicity. This work proposes a novel bio-based approach to obtain hydrophobic cotton. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:60-65, 2016 [ABSTRACT FROM AUTHOR]

Several works have been proposed in the last few years for raw trajectory data analysis, and some attempts have been made to define trajectories from a more semantic point of view. Semantic trajectory data analysis has received significant attention recently, but the formal definition of semantic trajectory, the set of aspects that should be considered to semantically enrich trajectories and a conceptual data model integrating these aspects from a broad sense is still missing. This article presents a semantic trajectory conceptual data model named CONSTAnT, which defines the most important aspects of semantic trajectories. We believe that this model will be the foundation for the design of semantic trajectory databases, where several aspects that make a trajectory 'semantic' are taken into account. The proposed model includes the concepts of semantic subtrajectory, semantic points, geographical places, events, goals, environment and behavior, to create a general concept of semantic trajectory. The proposed model is the result of several years of work by the authors in an effort to add more semantics to raw trajectory data for real applications. Two application examples and different queries show the flexibility of the model for different domains. [ABSTRACT FROM AUTHOR]