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Despite advances in precision oncology, cancer remains a global public health issue. In this report, proof‐of‐principle evidence is presented that a cell‐penetrable peptide (ACP52C) dissociates transcription factor CP2c complexes and induces apoptosis in most CP2c oncogene‐addicted cancer cells through transcription activity‐independent mechanisms. CP2cs dissociated from complexes directly interact with and degrade YY1, leading to apoptosis via the MDM2‐p53 pathway. The liberated CP2cs also inhibit TDP2, causing intrinsic genome‐wide DNA strand breaks and subsequent catastrophic DNA damage responses. These two mechanisms are independent of cancer driver mutations but are hindered by high MDM2 p60 expression. However, resistance to ACP52C mediated by MDM2 p60 can be sensitized by CASP2 inhibition. Additionally, derivatives of ACP52C conjugated with fatty acid alone or with a CASP2 inhibiting peptide show improved pharmacokinetics and reduced cancer burden, even in ACP52C‐resistant cancers. This study enhances the understanding of ACP52C‐induced cancer‐specific apoptosis induction and supports the use of ACP52C in anticancer drug development. [ABSTRACT FROM AUTHOR]

In vitro permeation tests (IVPT) offer accurate and cost‐effective development pathways for locally acting drugs, such as topical dermatological products. For assessment of bioequivalence, the FDA draft guidance on generic acyclovir 5% cream introduces a new experimental design, namely the single‐dose, multiple‐replicate per treatment group design, as IVPT pivotal study design. We examine the statistical properties of its hypothesis testing method—namely the mixed scaled average bioequivalence (MSABE). Meanwhile, some adaptive design features in clinical trials can help researchers make a decision earlier with fewer subjects or boost power, saving resources, while controlling the impact on family‐wise error rate. Therefore, we incorporate MSABE in an adaptive design combining the group sequential design and sample size re‐estimation. Simulation studies are conducted to study the passing rates of the proposed methods—both within and outside the average bioequivalence limits. We further consider modifications to the adaptive designs applied for IVPT BE trials, such as Bonferroni's adjustment and conditional power function. Finally, a case study with real data demonstrates the advantages of such adaptive methods. [ABSTRACT FROM AUTHOR]

Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles. Several protocols have been developed to derive MuSC‐like progenitors from human induced pluripotent stem (iPS) cells (hiPSCs) to establish a source of myogenic cells with controllable proliferation and differentiation. However, current hiPSC myogenic derivatives also suffer from limitations of cell migration, ultimately delaying their clinical translation. Here we use a multi‐disciplinary approach including bioinformatics and tissue engineering to show that DLL4 and PDGF‐BB improve migration of hiPSC‐derived myogenic progenitors. Transcriptomic analyses demonstrate that this property is conserved across species and multiple hiPSC lines, consistent with results from single cell motility profiling. Treated cells showed enhanced trans‐endothelial migration in transwell assays. Finally, increased motility was detected in a novel humanised assay to study cell migration using 3D artificial muscles, harnessing advanced tissue modelling to move hiPSCs closer to future muscle gene and cell therapies. Synopsis: This study describes an advanced framework to assess, model and enhance migration of human skeletal myogenic progenitor cells using platforms including induced pluripotent stem (iPS) cells, RNAseq, single cell profiling and tissue engineering. Activation of NOTCH and platelet‐derived growth factor (PDGF) signalling pathways induces conserved transcriptional changes in mouse and human tissue‐ and iPS cell‐derived myogenic progenitors (hiMPs).Combined activation of NOTCH and PDGF signalling via Delta‐like 4 (DLL4) and PDGF‐BB treatment assessed via single cell migratory profiling results in enhanced motility of hiMPs.Organ‐on‐chip and transwell assays show increased trans‐endothelial migration of treated hiMPs not mediated by enhanced adhesion.A novel 3D assay modelling intramuscular migration using engineered muscles shows increased motility of treated hiMPs in a biomimetic environment. [ABSTRACT FROM AUTHOR]

Directional cell migration is a critical process underlying morphogenesis and post‐natal tissue regeneration. During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post‐natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. Additionally, safe and efficient migration of transplanted cells is critical in cell therapies, both allogeneic and autologous. Although various myogenic cell types have been administered intramuscularly or intravascularly, functional restoration has not been achieved yet in patients with degenerative diseases affecting multiple large muscles. One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post‐natal regeneration. Furthermore, strategies utilised to improve migratory capacity of myogenic cells are examined in order to identify potential treatments that may be applied to future transplantation protocols. [ABSTRACT FROM AUTHOR]

Polarization‐type potential‐induced degradation (PID‐p) of photovoltaic (PV) modules composed of n‐type interdigitated back contact (IBC) solar cells can decrease the performance of the PV modules under positive potential conditions. The purpose of this study is to observe and describe the reduction and stabilization of the electrical performance of every PV module installed in an operating PV system with the progress of the PID‐p over a time duration of 6 years. The direct current circuit of the investigated PV system is not grounded. Therefore, all the PV strings are under floating potential conditions. The results show that the performance of the n‐type IBC PV modules exhibits a two‐step degradation. In the first step of degradation, the PV performance at positive potential decreases with increasing the PV module potential, whereas the PV modules at negative potential show little performance degradation. The PV performance reduction due to PID‐p is mainly caused by the decreases in both open‐circuit voltage (VOC) and short circuit current (ISC). A PV performance reduction, which principally arises from the decrease in ISC, is observed at every PV module in the PV strings regardless of the PV module potential in the second step of degradation. Therefore, solar cell manufacturers who produce n‐type IBC, as well as those producing passivated emitters and rear totally diffused solar cells, are advised that processes to prevent the PID‐p are required. [ABSTRACT FROM AUTHOR]

In this article, we described a development of full Roll‐to‐Roll (R2R) process for flexible display backplane. For many years, we made an effort to convert rigid glass substrates to flexible films for thinness, light weight, flexibility and design freedom. Through Government support program, we have built and stabilized R2R facilities for many years. Amorphous indium‐gallium‐zinc oxide thin‐film transistors (TFTs) with coplanar structure are fabricated using roll type Polyimide film. We have demonstrated the world's first 20 inch flexible oxide TFTs backplane with SVGA (800x600, 50ppi) resolution), which can drive Electrophoretic paper. The process flow and key technologies to fabricate a‐InGaZnO flexible backplane by R2R process will be discussed. [ABSTRACT FROM AUTHOR]

In this paper, we consider the problem of testing nonequivalence of several independent normal population means. It is a well-known problem to test the equality of several means using the analysis of variance (ANOVA). Instead of determining the equality, one may consider more flexible homogeneity, which allows a predetermined level of difference. This problem is known as testing nonequivalence of populations. We propose the plug-in statistics for two different measures of variability: the sum of the absolute deviations and the maximum of the absolute deviations. For each test, the least favorable configuration (LFC) to ensure the maximum rejection probability under the null hypothesis is investigated. Furthermore, we demonstrate the numerical studies based on both simulation and real data to evaluate the plug-in tests and compare these with the range test. [ABSTRACT FROM AUTHOR]

In this article, we described a roll-to-roll (R2R) deposition processed amorphous indium-gallium-zinc oxide (a-IGZO) thin-film transistors (TFTs), depending on the source/drain (S/D) offset length from 10 to 80μm equally at S/D each side. It was fabricated at a maximum temperature under 250°C. Increasing length of S/D offset makes the device performance be lowered, but additional process at the offset region can minimize this problem. We demonstrated that the R2R deposition processed TFTs exhibited competitive device characteristics with large S/D offset (∼80μm), including an averaged field-effect mobility of 3.06cm2/Vs, an on-to-off current ratio of ∼107, a threshold voltage of 1.33V, and a subthreshold gate swing of 0.54V/dec. [ABSTRACT FROM AUTHOR]

Accurate depth estimation is a challenging, yet essential step in the conversion of a 2D image sequence to a 3D stereo sequence. We present a novel approach to construct a temporally coherent depth map for each image in a sequence. The quality of the estimated depth is high enough for the purpose of2D to 3D stereo conversion. Our approach first combines the video sequence into a panoramic image. A user can scribble on this single panoramic image to specify depth information. The depth is then propagated to the remainder of the panoramic image. This depth map is then remapped to the original sequence and used as the initial guess for each individual depth map in the sequence. Our approach greatly simplifies the required user interaction during the assignment of the depth and allows for relatively free camera movement during the generation of a panoramic image. We demonstrate the effectiveness of our method by showing stereo converted sequences with various camera motions. [ABSTRACT FROM AUTHOR]

Computer graphics is one of the most efficient ways to create a stereoscopic image. The process of stereoscopic CG generation is, however, still very inefficient compared to that of monoscopic CG generation. Despite that stereo images are very similar to each other, they are rendered and manipulated independently. Additional requirements for disparity control specific to stereo images lead to even greater inefficiency. This paper proposes a method to reduce the inefficiency accompanied in the creation of a stereoscopic image. The system automatically generates an optimized single image representation of the entire visible area from both cameras. The single image can be easily manipulated with conventional techniques, as it is spatially smooth and maintains the original shapes of scene objects. In addition, a stereo image pair can be easily generated with an arbitrary disparity setting. These convenient and efficient features are achieved by the automatic generation of a stereo camera pair, robust occlusion detection with a pair of Z-buffers, an optimization method for spatial smoothness, and stereo image pair generation with a non-linear disparity adjustment. Experiments show that our technique dramatically improves the efficiency of stereoscopic image creation while preserving the quality of the results. [ABSTRACT FROM AUTHOR]

ABSTRACT For topical, dermatological drug products, an in vitro option to determine bioequivalence (BE) between test and reference products is recommended. In particular, in vitro permeation test (IVPT) data analysis uses a reference‐scaled approach for two primary endpoints, cumulative penetration amount (AMT) and maximum flux (Jmax), which takes the within donor variability into consideration. In 2022, the Food and Drug Administration (FDA) published a draft IVPT guidance that includes statistical analysis methods for both balanced and unbalanced cases of IVPT study data. This work presents a comprehensive evaluation of various methodologies used to estimate critical parameters essential in assessing BE. Specifically, we investigate the performance of the FDA draft IVPT guidance approach alongside alternative empirical and model‐based methods utilizing mixed‐effects models. Our analyses include both simulated scenarios and real‐world studies. In simulated scenarios, empirical formulas consistently demonstrate robustness in approximating the true model, particularly in effectively addressing treatment–donor interactions. Conversely, the effectiveness of model‐based approaches heavily relies on precise model selection, which significantly influences their results. The research emphasizes the importance of accurate model selection in model‐based BE assessment methodologies. It sheds light on the advantages of empirical formulas, highlighting their reliability compared to model‐based approaches and offers valuable implications for BE assessments. Our findings underscore the significance of robust methodologies and provide essential insights to advance their understanding and application in the assessment of BE, employed in IVPT data analysis. [ABSTRACT FROM AUTHOR]