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Whether in performing arts, sporting, or everyday contexts, when we watch others move, we tend to enjoy bodies moving in synchrony. Our enjoyment of body movements is further enhanced by our own prior experience with performing those movements, or our 'embodied experience'. The relationships between movement synchrony and enjoyment, as well as embodied experience and movement enjoyment, are well known. The interaction between enjoyment of movements, synchrony, and embodiment is less well understood, and may be central for developing new approaches for enriching social interaction. To examine the interplay between movement enjoyment, synchrony, and embodiment, we asked participants to copy another person's movements as accurately as possible, thereby gaining embodied experience of movement sequences. Participants then viewed other dyads performing the same or different sequences synchronously, and we assessed participants' recognition of having performed these sequences, as well as their enjoyment of each movement sequence. We used functional near‐infrared spectroscopy to measure cortical activation over frontotemporal sensorimotor regions while participants performed and viewed movements. We found that enjoyment was greatest when participants had mirrored the sequence and recognised it, suggesting that awareness of embodiment may be central to enjoyment of synchronous movements. Exploratory analyses of relationships between cortical activation and enjoyment and recognition implicated the sensorimotor cortices, which subserve action observation and aesthetic processing. These findings hold implications for clinical research and therapies seeking to foster successful social interaction. [ABSTRACT FROM AUTHOR]

Nucleocytoplasmic transport regulates the passage of proteins between the nucleus and cytoplasm. In the best characterized pathway, importin (IMP) α bridges cargoes bearing basic, classical nuclear localization signals (cNLSs) to IMPβ1, which mediates transport through the nuclear pore complex. IMPα recognizes three types of cNLSs via two binding sites: the major binding site accommodates monopartite cNLSs, the minor binding site recognizes atypical cNLSs, while bipartite cNLSs simultaneously interact with both major and minor sites. Despite the growing knowledge regarding IMPα‐cNLS interactions, our understanding of the evolution of cNLSs is limited. We combined bioinformatic, biochemical, functional, and structural approaches to study this phenomenon, using polyomaviruses (PyVs) large tumor antigens (LTAs) as a model. We characterized functional cNLSs from all human (H)PyV LTAs, located between the LXCXE motif and origin binding domain. Surprisingly, the prototypical SV40 monopartite NLS is not well conserved; HPyV LTA NLSs are extremely heterogenous in terms of structural organization, IMPα isoform binding, and nuclear targeting abilities, thus influencing the nuclear accumulation properties of full‐length proteins. While several LTAs possess bipartite cNLSs, merkel cell PyV contains a hybrid bipartite cNLS whose upstream stretch of basic amino acids can function as an atypical cNLS, specifically binding to the IMPα minor site upon deletion of the downstream amino acids after viral integration in the host genome. Therefore, duplication of a monopartite cNLS and subsequent accumulation of point mutations, optimizing interaction with distinct IMPα binding sites, led to the evolution of bipartite and atypical NLSs binding at the minor site. [ABSTRACT FROM AUTHOR]

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Human cytomegalovirus DNA polymerase processivity factor UL44 is transported into the nucleus by importin (IMP) α/β through a classical nuclear localization signal (NLS), and this region is susceptible to cdc2‐mediated phosphorylation at position T427. Whilst phosphorylation within and close to the UL44 NLS regulates nuclear transport, the details remain elusive, due to the paucity of structural information regarding the role of negatively charged cargo phosphate groups. We addressed this issue by studying the effect of UL44 T427 phosphorylation on interaction with several IMPα isoforms by biochemical and structural approaches. Phosphorylation decreased UL44/IMPα affinity 10‐fold, and a comparative structural analysis of UL44 NLS phosphorylated and non‐phosphorylated peptides complexed with mouse IMPα2 revealed the structural rearrangements responsible for phosphorylation‐dependent inhibition of UL44 nuclear import. [ABSTRACT FROM AUTHOR]

Background: Use of diets with increased concentrations of dietary fiber is thought to be beneficial in the management of dogs with large bowel diarrhea. Objective: To determine whether feeding a diet with high concentrations of soluble and insoluble fiber to dogs with acute colitis would be superior to feeding a diet with typical fiber levels. Animals: A total of 52 dogs with acute signs of large bowel diarrhea housed in an animal shelter were entered into the study; 11 dogs per diet completed the protocol. Methods: In this randomized, prospective study, dogs with a fecal score of 4, 5, 6, or 7 and signs of acute colitis were fed a high fiber diet (4.54% soluble; 15.16% insoluble fiber) or a standard diet (0.6% soluble; 5.33% insoluble fiber) and fecal scores compared over the course of the study with significance defined as P <.05. Results: All dogs fed the high fiber diet (11/11; 100%) had a fecal score <5 on the day of adoption or day 9, which was statistically different (P <.04) than dogs fed the standard diet (6/11 dogs; 55%; 95% CI: 23‐83). The proportions of stools with a fecal score >4 were greater (P =.0001) in the dogs fed the standard diet (29/48 samples; 60%; 95% CI: 45‐74) compared to the high fiber diet (8/50 samples; 16%; 95% CI: 7‐29). Conclusions and Clinical Importance: The results support feeding the high fiber diet described herein to dogs with acute large bowel diarrhea. [ABSTRACT FROM AUTHOR]

The process of understanding the minds of other people, such as their emotions and intentions, is mimicked when individuals try to understand an artificial mind. The assumption is that anthropomorphism, attributing human‐like characteristics to non‐human agents and objects, is an analogue to theory‐of‐mind, the ability to infer mental states of other people. Here, we test to what extent these two constructs formally overlap. Specifically, using a multi‐method approach, we test if and how anthropomorphism is related to theory‐of‐mind using brain (Experiment 1) and behavioural (Experiment 2) measures. In a first exploratory experiment, we examine the relationship between dispositional anthropomorphism and activity within the theory‐of‐mind brain network (n = 108). Results from a Bayesian regression analysis showed no consistent relationship between dispositional anthropomorphism and activity in regions of the theory‐of‐mind network. In a follow‐up, pre‐registered experiment, we explored the relationship between theory‐of‐mind and situational and dispositional anthropomorphism in more depth. Participants (n = 311) watched a short movie while simultaneously completing situational anthropomorphism and theory‐of‐mind ratings, as well as measures of dispositional anthropomorphism and general theory‐of‐mind. Only situational anthropomorphism predicted the ability to understand and predict the behaviour of the film's characters. No relationship between situational or dispositional anthropomorphism and general theory‐of‐mind was observed. Together, these results suggest that while the constructs of anthropomorphism and theory‐of‐mind might overlap in certain situations, they remain separate and possibly unrelated at the personality level. These findings point to a possible dissociation between brain and behavioural measures when considering the relationship between theory‐of‐mind and anthropomorphism. [ABSTRACT FROM AUTHOR]

Background: In Australian health care, the consistent rise in demand for orthopaedic outpatient clinic services is creating marked challenges in the provision of quality care. This study investigates the efficacy and safety of a virtual fracture clinic (VFC) as an alternative model of care for the management of acute injuries and musculoskeletal conditions in the Australian public hospital setting. Methods: A retrospective cohort study of consecutive emergency department (ED) referrals to the Department of Orthopaedic Surgery was conducted comparing outcomes prior to (November 2015–February 2017) and after (March 2017–June 2018) implementation of a VFC. The primary outcome measures assessed were the proportion of referrals virtually discharged and unplanned 30‐day ED re‐attendance rates. Results: A total of 737 (36.4%) referrals managed by the VFC were discharged without requiring orthopaedic outpatient clinic attendance. The rate of unplanned ED re‐attendances was 5.2% post‐VFC implementation compared to 6.5% at baseline (P = 0.01). VFC implementation was also associated with reductions in the average number of orthopaedic outpatient clinic attendances per referral (1.1 versus 1.7, P < 0.01) and the number of referrals lost to follow‐up (7.2% versus 14.7%, P < 0.01). In addition, patient wait times for first contact by the orthopaedic team were significantly reduced from a median of 7 (IQR 5, 9) days to 2 (IQR 1, 3) days post‐intervention (P < 0.01). No complications or adverse events were reported. Conclusion: This study demonstrates that a VFC is applicable to the Australian healthcare system, and can lead to effective and safe provision of orthopaedic outpatient care. [ABSTRACT FROM AUTHOR]

Ambivalent sexism theory recognizes that sexist attitudes maintain gender inequalities via sociocultural and close relationship processes. This review advances established work on sociocultural processes by showing how people's need for relationship security is also central to the sources and functions of sexism. Men's hostile sexism—overtly derogatory attitudes toward women—involves insecurities about women exploiting men's relational dependence. Accordingly, men's hostile sexism predicts relational aggression when their dependence on partners is risky. Men's benevolent sexism—patronizing and protective attitudes toward women—offers men relationship security by idealizing traditional gender roles in romantic relationships. Benevolent sexism also appeals to women who seek the security of a devoted partner, but requires women to invest in their relationship at the expense of their independence. Our relationship science perspective reveals that romantic (in)security is critical to why people adopt sexist attitudes and why sexist attitudes create differential costs and benefits for women and men. [ABSTRACT FROM AUTHOR]

Men's hostile sexism promotes aggressive attitudes, motivations and behaviors toward women. Despite the costs these effects should have for women, prior research has failed to test how men's hostile sexism predicts the problems women experience in important domains. We address this oversight by utilizing dyadic data from 363 heterosexual couples to test how male partners' hostile sexism predicts women's relationship experiences and evaluations. Male partners' hostile sexism was associated with women experiencing more severe problems across a greater number of domains. Moreover, the areas experienced as most problematic were consistent with the power, dependence, and trust concerns underlying men's hostile sexism, including problems with power dynamics, jealousy, and serious problems involving gender‐role conflict, abuse, infidelity and alcohol/drugs. The greater problems associated with male partners' hostile sexism predicted more negative relationship evaluations for women. These results demonstrate the importance of examining how men's hostile sexism harms women in important life domains. [ABSTRACT FROM AUTHOR]

Abstract: Understanding the mechanisms and consequences of attributing socialness to artificial agents has important implications for how we can use technology to lead more productive and fulfilling lives. Here, we integrate recent findings on the factors that shape behavioral and brain mechanisms that support social interactions between humans and artificial agents. We review how visual features of an agent, as well as knowledge factors within the human observer, shape attributions across dimensions of socialness. We explore how anthropomorphism and dehumanization further influence how we perceive and interact with artificial agents. Based on these findings, we argue that the cognitive reconstruction within the human observer is likely to be far more crucial in shaping our interactions with artificial agents than previously thought, while the artificial agent's visual features are possibly of lesser importance. We combine these findings to provide an integrative theoretical account based on the “like me” hypothesis, and discuss the key role played by the Theory‐of‐Mind network, especially the temporal parietal junction, in the shift from mechanistic to social attributions. We conclude by highlighting outstanding questions on the impact of long‐term interactions with artificial agents on the behavioral and brain mechanisms of attributing socialness to these agents. [ABSTRACT FROM AUTHOR]

Successful social interactions depend on the ability to anticipate other people's actions. Current conceptualizations of brain function propose that causes of sensory input are inferred through their integration with internal predictions generated in the observer's motor system during action observation. Less is known concerning how action prediction changes with age. Previously we showed that internal action representations are less specific in older compared with younger adults at behavioral and neural levels. Here, we characterize how neural activity varies while healthy older adults aged 56-71 years predict the time-course of an unfolding action as well as the relation to task performance. By using fMRI, brain activity was measured while participants observed partly occluded actions and judged the temporal coherence of the action continuation that was manipulated. We found that neural activity in frontoparietal and occipitotemporal regions increased the more an action continuation was shifted backwards in time. Action continuations that were shifted towards the future preferentially engaged early visual cortices. Increasing age was associated with neural activity that extended from posterior to anterior regions in frontal and superior temporal cortices. Lower sensitivity in action prediction resulted in activity increases in the caudate. These results imply that the neural implementation of predicting actions undergoes similar changes as the neural process of executing actions in older adults. The comparison between internal predictions and sensory input seems to become less precise with age leading to difficulties in anticipating observed actions accurately, possibly due to less specific internal action models. [ABSTRACT FROM AUTHOR]

Previous research on aesthetic preferences demonstrates that people are more likely to judge a stimulus as pleasing if it is familiar. Although general familiarity and liking are related, it is less clear how motor familiarity, or embodiment, relates to a viewer's aesthetic appraisal. This study directly compared how learning to embody an action impacts the neural response when watching and aesthetically evaluating the same action. Twenty-two participants trained for 4 days on dance sequences. Each day they physically rehearsed one set of sequences, passively watched a second set, listened to the music of a third set, and a fourth set remained untrained. Functional MRI was obtained prior to and immediately following the training period, as were affective and physical ability ratings for each dance sequence. This approach enabled precise comparison of self-report methods of embodiment with nonbiased, empirical measures of action performance. Results suggest that after experience, participants most enjoy watching those dance sequences they danced or observed. Moreover, brain regions involved in mediating the aesthetic response shift from subcortical regions associated with dopaminergic reward processing to posterior temporal regions involved in processing multisensory integration, emotion, and biological motion. [ABSTRACT FROM AUTHOR]

Linking observed and executable actions appears to be achieved by an action observation network (AON), comprising parietal, premotor, and occipitotemporal cortical regions of the human brain. AON engagement during action observation is thought to aid in effortless, efficient prediction of ongoing movements to support action understanding. Here, we investigate how the AON responds when observing and predicting actions we cannot readily reproduce before and after visual training. During pre- and posttraining neuroimaging sessions, participants watched gymnasts and wind-up toys moving behind an occluder and pressed a button when they expected each agent to reappear. Between scanning sessions, participants visually trained to predict when a subset of stimuli would reappear. Posttraining scanning revealed activation of inferior parietal, superior temporal, and cerebellar cortices when predicting occluded actions compared to perceiving them. Greater activity emerged when predicting untrained compared to trained sequences in occipitotemporal cortices and to a lesser degree, premotor cortices. The occipitotemporal responses when predicting untrained agents showed further specialization, with greater responses within body-processing regions when predicting gymnasts' movements and in object-selective cortex when predicting toys' movements. The results suggest that (1) select portions of the AON are recruited to predict the complex movements not easily mapped onto the observer's body and (2) greater recruitment of these AON regions supports prediction of less familiar sequences. We suggest that the findings inform both the premotor model of action prediction and the predictive coding account of AON function. Hum Brain Mapp, 2013. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

As humans, we gather a wide range of information about other people from watching them move. A network of parietal, premotor, and occipitotemporal regions within the human brain, termed the action observation network (AON), has been implicated in understanding others' actions by means of an automatic matching process that links observed and performed actions. Current views of the AON assume a matching process biased towards familiar actions; specifically, those performed by conspecifics and present in the observer's motor repertoire. In this study, we test how this network responds to form and motion cues when observing natural human motion compared to rigid robotic-like motion across two independent functional neuroimaging experiments. In Experiment 1, we report the surprising finding that premotor, parietal, occipitotemporal regions respond more robustly to rigid, robot-like motion than natural human motion. In Experiment 2, we replicate and extend this finding by demonstrating that the same pattern of results emerges whether the agent is a human or a robot, which suggests the preferential response to robot-like motion is independent of the agent's form. These data challenge previous ideas about AON function by demonstrating that the core nodes of this network can be flexibly engaged by novel, unfamiliar actions performed by both human and non-human agents. As such, these findings suggest that the AON is sensitive to a broader range of action features beyond those that are simply familiar. Hum Brain Mapp 33:2238-2254, 2012. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Blood vessel/epicardial substance (Bves) is a transmembrane protein that influences cell adhesion and motility through unknown mechanisms. We have discovered that Bves directly interacts with VAMP3, a SNARE protein that facilitates vesicular transport and specifically recycles transferrin and β-1-integrin. Two independent assays document that cells expressing a mutated form of Bves are severely impaired in the recycling of these molecules, a phenotype consistent with disruption of VAMP3 function. Using Morpholino knockdown in Xenopus laevis, we demonstrate that elimination of Bves function specifically inhibits transferrin receptor recycling, and results in gastrulation defects previously reported with impaired integrin-dependent cell movements. Kymographic analysis of Bves-depleted primary and cultured cells reveals severe impairment of cell spreading and adhesion on fibronectin, indicative of disruption of integrin-mediated adhesion. Taken together, these data demonstrate that Bves interacts with VAMP3 and facilitates receptor recycling both in vitro and during early development. Thus, this study establishes a newly identified role for Bves in vesicular transport and reveals a novel, broadly applied mechanism governing SNARE protein function. [ABSTRACT FROM AUTHOR]

Observation of human actions recruits a well-defined network of brain regions, yet the purpose of this action observation network (AON) remains under debate. Some authors contend that this network has developed to respond specifically to observation of human actions. Conversely, others suggest that this network responds in a similar manner to actions prompted by human and non-human cues, and that one’s familiarity with the action is the critical factor that drives this network. Previous studies investigating human and non-human action cues often confound novelty and stimulus form. Here, we used a dance-learning paradigm to assess AON activity during observation of trained and untrained dance cues where a human model was present or absent. Results show that individual components of the AON respond differently to the human form and to dance training. The bilateral superior temporal cortex responds preferentially to videos with a human present, regardless of training experience. Conversely, the right ventral premotor cortex responds more strongly when observing sequences that had been trained, regardless of the presence of a human. Our findings suggest that the AON comprises separate and dissociable components for motor planning and observing other people’s actions. [ABSTRACT FROM AUTHOR]

Abstract: Benevolent sexism prescribes that men should cherish and protect women in intimate relationships. Despite the romantic tone of these attitudes, prior research indicates that benevolent sexism undermines women's competence, ambition, and independence. Ambivalent sexism theory proposes that benevolent sexism is able to incur these costs because the promise of a chivalrous and protective partner offers women security in their intimate relationships. We tested this key proposition by examining whether women who intensely need relationship security—women higher in attachment anxiety—are more attracted to men who endorse benevolent sexism. Highly anxious women (N = 632) rated men described as endorsing benevolent sexism as relatively more attractive, and reported greater preferences for partners to hold benevolently sexist attitudes. These results advance understanding regarding the underlying reasons women find benevolent sexism appealing and identify who will be most vulnerable to the potential costs of benevolent sexism. [ABSTRACT FROM AUTHOR]