1. Modifying Fluorescent Protein Chromophore to Detect Protamine and Application in Real Serum Samples.
- Author
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Gao, Xiaochen, Li, Ke, Xiao, Yu, Dai, Jianan, Shen, Zheyu, Lv, Guangping, and Yang, Yaqiong
- Subjects
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CHARGE transfer , *CARBOXYL group , *CARBONYL group , *DETECTION limit , *GREEN fluorescent protein - Abstract
As effective methods to accurately and rapidly detect protamine remains a major challenge, here we present a fluorescent protein chromophore‐based probe (IDL‐FP) utilizing twisted‐intramolecular charge transfer (TICT) to detect protamine. The introduction of an electron‐donor group 4‐dimethylaminobenzaldehyde extends the emission wavelength of IDL‐FP to the red region. The deprotection of t‐butyloxy carbonyl groups can then generate an electronegative carboxyl group, which promotes the binding to protamine via electrostatic attraction. Thus, IDL‐FP is sensitive and specific towards protamine and can serve as a highly selective detector for protamine with a detection limit as low as 8.87 ng/mL. As IDL‐FP exhibited good stability in physiological pH environment, we successfully apply IDL‐FP to detect spiked‐in protamine in human serum. In summary, probe IDL‐FP is a promising tool for the quantitative determination of protamine. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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