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Background: Trigeminal neuralgia (TN) is a neuropathic pain that occurs in branches of the trigeminal nerve. MicroRNAs (miRNAs) have been considered key mediators of neuropathic pain. This study was aimed to elucidate the pathophysiological function and mechanisms of miR‐223‐3p in mouse models of TN. Methods: Infraorbital nerve chronic constriction injury (CCI‐ION) was applied in male C57BL/6J mice to establish mouse models of TN. Pain responses were assessed utilizing Von Frey method. The expression of miR‐223‐3p, MKNK2, and MAPK/ERK pathway protein in trigeminal ganglions (TGs) of CCI‐ION mice was measured using RT‐qPCR and Western blotting. The concentrations of inflammatory cytokines were evaluated using Western blotting. The relationship between miR‐223‐3p and MKNK2 was tested by a luciferase reporter assay. Results: We found that miR‐223‐3p was downregulated, while MKNK2 was upregulated in TGs of CCI‐ION mice. MiR‐223‐3p overexpression by an intracerebroventricular injection of Lv‐miR‐223‐3p attenuated trigeminal neuropathic pain in CCI‐ION mice, as well as reduced the protein levels of pro‐inflammatory cytokines in TGs of CCI‐ION mice. MKNK2 was verified to be targeted by miR‐223‐3p. Additionally, miR‐223‐3p overexpression decreased the phosphorylation levels of ERK1/2, JNK, and p38 protein in TGs of CCI‐ION mice to inhibit MAPK/ERK signaling. Conclusions: Overall, miR‐223‐3p attenuates the development of TN by targeting MKNK2 to suppress MAPK/ERK signaling. [ABSTRACT FROM AUTHOR]

Objective: The aim of the present study was to summarize the clinical efficacy of three‐dimensional (3D) printing technology combined with the Masquelet technique in the treatment of calcaneal defects. Methods: From January 2018 to April 2019, 3D printing combined with induced masquelet technology was used to treat four patients with calcaneal defects, including two men and two women. The patients were aged 22–52 years old, with an average age of 36 years. There were two cases of traffic accident injuries, there was one case of a fall from height, and there was one case of crush injury. CT scans were used to reconstruct the bilateral calcaneus, mirror technology was used to construct the bone defect area, and Materialise 3‐matic software was used to design the calcaneus shaper mold and 3D print the mold. During the operation, the mold was used to shape the bone cement and fill the bone defect. In the second stage, the bone cement was removed and autologous bone was implanted to repair the bone defect. All patients were followed up to observe the effect. Results: All four patients were followed up for 14 months (range, 10–18 months). There were three cases of infectious bone defects: two cases of Escherichia coli and one case of Pseudomonas aeruginosa. The 3D printed mold was used to shape the bone cement. During the operation, it was found to have a high degree of matching with the defect area of calcaneus. There is no need to adjust it again, and the wound healed well after the first stage. In the second stage of surgery, it was found that the induced membrane formed was complete and of appropriate size; the bone cement was easily removed during the operation. The fracture healing time was 3–6 months, with an average of 4 months. At the last follow up, there was no pain and the patients walked with full weight bearing. The Maryland score was 94 points (range, 88–98 points); three cases were excellent and one case was good. The AOFAS score ranged from 86 to 98, with an average of 92.8 points; three cases were excellent and one case was good. Conclusion: Three‐dimensional printing technology combined with induced membrane technology is an effective approach for treating calcaneal bone defects. [ABSTRACT FROM AUTHOR]

Summary: The fiber‐wireless (FiWi) access network is a very promising solution for next‐generation access networks. Because of the different protocols between its subnets, it is hard to globally optimize the operation of FiWi networks. Network virtualization technology is applied to FiWi networks to realize the coexistence of heterogeneous networks and centralized control of network resource. The existing virtual resource management methods always be designed to optimize virtual network (VN) request acceptance rate and survivability, but seldom consider energy consumption and varied requirements of quality of service (QoS) satisfaction, which is a hot and important topic in the industrial field. Therefore, this paper focuses on the QoS‐aware cross‐domain collaborative energy saving mechanism for FiWi virtual networks. First, the virtual network embedding (VNE) model, energy consumption model, and VNE profit model of FiWi networks are established. Then, a QoS‐aware in‐region VN embedding mechanism is proposed to guarantee service quality of different services. After that, an underlying resource updating mechanism based on energy efficiency awareness is designed to realize low‐load ONU and wireless routers co‐sleep in FiWi networks. Finally, a QoS‐aware re‐embedding mechanism is presented to allocate proper resource to the VNs affected by the sleeping mechanism. Especially for video VNs, a re‐embedding scheme which adopts traffic splitting and multipath route is introduced to meet resource limitation and low latency. Simulation results show that the proposed mechanism can reduce FiWi network's energy consumption, improve VNE profit, and ensure high embedding accepting rate and strict delay demand of high‐priority VNs. [ABSTRACT FROM AUTHOR]