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Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion ( DRG) and the osteoblast-like MC3T3-E1 cells using an in vitro coculture system. Our findings indicate that signal transduction from DRG-derived neurons to MC3T3-E1 cells is suppressed by antagonists of the AMPA receptor and the NK1 receptor. Conversely, signal transduction from MC3T3-E1 cells to DRG-derived neurons is suppressed by a P2X7 receptor antagonist. Our results suggest that these cells communicate with each other by exocytosis of glutamate, substance P in the efferent signal, and ATP in the afferent signal. [ABSTRACT FROM AUTHOR]

Background and Purpose: The sympathetic nervous system regulates bone remodelling, in part, through ß2 -adrenoceptor signalling. However, the physiological role of α1 -adrenoceptor signalling in bone in vivo remains unclear. Therefore, to obtain a deeper understanding of bone remodelling by the sympathetic nervous system, we investigated the role of α1B -adrenoceptor signalling in bone metabolism.Experimental Approach: Prazosin, a nonspecific α1 -adrenoceptor antagonist, was administered for 2 weeks in C57BL6 mice, and efficacy was evaluated by bone microarchitecture using microcomputed tomography and determination of bone formation by fluorescent labelling of bone. We also compared the bone phenotype of α1B -adrenoceptor null mice (α1B (-/-) ) with that of wild-type littermates.Key Results: We demonstrated that the systemic administration of prazosin decreased bone formation. In addition, α1B -adrenoceptor-deficient mice had a lower bone mass due to decreased bone formation but did not exhibit any changes in bone-resorbing activity. Furthermore, stimulation with phenylephrine, a non-specific α1 -adrenoceptor agonist, increased the expression of the transcriptional factor CCAAT/enhancer-binding protein δ (Cebpd) in MC3T3-E1 osteoblastic cells. The overexpression of Cebpd induced cellular proliferation in MC3T3-E1 cells, whereas the silencing of Cebpd suppressed it.Conclusions and Implications: Taken together, these results suggested that α1B -adrenoceptor signalling is required for bone formation and regulated cellular proliferation through a mechanism relevant to the up-regulation of Cebpd in osteoblasts and, thus, provide new evidence for the physiological importance of α1B -adrenoceptor signalling in bone homeostasis. [ABSTRACT FROM AUTHOR]

Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to elucidate the possible functional role of D-Ser in chondrogenesis. Expression of mRNA and corresponding protein was seen for SR in cultured rat costal chondrocytes, while the addition of L-Ser significantly increased intracellular and extracellular levels of D-Ser. In organotypic cultured mouse embryonic metatarsals isolated before vascularization, SR mRNA was highly localized in hypertrophic and calcified chondrocytes. Exposure to D-Ser not only suppressed several chondrocytic maturation markers, including alkaline phosphatase (ALP) activity, Ca2+ accumulation, nodule formation, and osteopontin expression, in rat chondrocytes, but also delayed chondral mineralization in mouse metatarsals. Either NMDA or Gly alone significantly increased Ca2+ accumulation in cultured chondrocytes, whereas D-Ser significantly prevented Ca2+ accumulation by Gly, but not by NMDA. Gly alone also significantly increased gene transactivation by the introduction of runt-related transcription factor-2 (Runx2) in COS7 cells transfected with NR1 and NR3A subunits, while D-Ser significantly prevented the increase by Gly without affecting the promoter activity of Runx2. In both cultured chondrocytes and metatarsals from NR1-null mice, significant decreases were seen in ALP activity and chondral mineralization, respectively. These results suggest that D-Ser may negatively regulate cellular differentiation through inhibiting NMDA receptors composed of NR1 and NR3A subunits in a manner related to Runx2 transcriptional activity in chondrocytes. J. Cell. Physiol. 220: 756–764, 2009. © 2009 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

Recently, the total number of distributed generators (DGs) such as photovoltaic generation system and wind turbine generation system connected to an actual distribution network has increased drastically. The distribution network connected to many distributed generators must be operated keeping reliability of power supply, power quality, and loss minimization. In order to accomplish active distribution network operation to take advantage of many connections of DGs, a new coordinated operation of distribution system with many connections of DGs is necessary. In this paper, the authors propose a coordinated operation of distribution network system connected to many DGs by using newly proposed sectionalizing switch control, sending voltage control, and computation of available DG connection capability. In order to check the validity of the proposed coordinated operation of distribution system, numerical simulations using the proposed coordinated distribution system operation are carried out in a practical distribution network model. © 2009 Wiley Periodicals, Inc. Electr Eng Jpn, 167(3): 46–57, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20655 [ABSTRACT FROM AUTHOR]

Probability distribution of surge discharging current of arresters provided a basis for conventional theory of grounding systems. In order to rationalize the grounding systems, it is necessary to grasp the statistical data of lightning surges on distribution lines caused by direct lightning strokes and indirect lightning strokes. Lightning phenomena on TEPCO's distribution lines had been continuously observed for the rationalization of lightning protection design of distribution lines. The observation had been carried out with still cameras and monitoring sensors of lightning surges. This makes it possible to discover new interesting facts that can be useful for rationalization of lightning protection design of distribution lines. Cumulative frequency distribution of conventional data is close to that of ZnO discharging current in the case of direct strokes and indirect strokes through TEPCO's observation. Moreover, to verify the cumulative current distribution in concrete poles, the authors have compared the cumulative distribution of current through ground lines with that of current through ground lines and concrete poles. The results show that the distribution of current through ground lines and concrete poles is larger than that of current through only ground lines for high currents exceeding 1 kA. This fact suggests that lightning surge current flows not only in ground lines but also in concrete poles. © 2008 Wiley Periodicals, Inc. Electr Eng Jpn, 165(2): 36–44, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20468 [ABSTRACT FROM AUTHOR]

In order to rationalize the lightning protection design of distribution lines, lightning phenomena on TEPCO's distribution lines were continuously observed. The observations were carried out with still cameras and lightning surge monitoring sensors. The results elucidate the lightning performance of distribution lines. For example, direct lightning strokes are the major cause of the present distribution line faults. This fact suggests that it is important to investigate observation data on direct lightning strokes. The observation data were analyzed by means of a direct lightning analysis model. The calculated waveforms were compared with the measured waveforms on distribution lines. The probability distributions of the surge currents and voltages were calculated by the Monte Carlo method. These results provide new data on lightning performance. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 91(10): 28– 37, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecj.10164 [ABSTRACT FROM AUTHOR]

For the rationalization of lightning protection design of distribution lines, it is important to clarify the behavior of distribution lines when direct or nearby lightning occurs. Because of the lower insulation level than for transmission lines, in studies on lightning protection design of distribution lines, not only direct lightning strokes but also induced voltages caused by nearby strokes must be taken into account. Thus, it is necessary to grasp the frequency of occurrence on lightning phenomena around distribution lines. For this aim, lightning phenomena on TEPCO's distribution lines in use had been continuously observed for 6 years (1996 to 2001). The observation was carried out in a composite way, using still cameras and sensor for acquisition of lightning surge waveform data. Through the observation, new phenomena about lightning performance on distribution lines in the field became apparent. In some cases, in spite of a direct strike to the line, flashover did not occur. This fact means that the distribution line has a certain level of lightning resistance. Moreover, it was confirmed that AC following current generated between both ends of insulator disappeared naturally. These results are interesting discoveries that can be useful in estimating the fault ratio precisely. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 157(1): 10–19, 2006; Published online in Wiley InterScience (http://www.interscience.wiley.com). DOI 10.1002/eej.20164 [ABSTRACT FROM AUTHOR]

Because of the lower insulation level than for transmission lines, in this study on lightning protection design of distribution lines, not only direct lightning strokes but also induced voltages caused by nearby strokes must be taken into account. Thus, it is necessary to grasp the frequency of occurrence of lightning phenomena around distribution lines. For this aim, lightning phenomena on TEPCO's distribution lines in use were continuously observed for 6 years (1996 to 2001). Through this observation, new interesting statistical data that can be a useful basis for rationalization of lightning protection design of distribution lines were obtained. Two hundred and four lightning strokes were observed through the six-year period. Probability of occurrence of a direct stroke was 22% (45 direct strokes were obtained). The fact that in 47% of direct strokes the electric outage did not occur is very interesting. Moreover, the value of surge discharge current of the arrester in the case of nearby stroke is the basis for conventional theory of grounding system. Therefore, statistical analysis of ZnO discharging current in the case of nearby strokes was carried out. As a result, in the case of nearby strokes, 5% value of cumulative frequency of discharging current is 0.5 kA. This value is half the conventional data. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(2): 8–16, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20180 [ABSTRACT FROM AUTHOR]

In order to elucidate mechanisms underlying modulation by static magnetism of the cellular functionality and/or integrity in the brain, we screened genes responsive to brief magnetism in cultured rat hippocampal neurons using differential display analysis. We have for the first time cloned and identified Ntan1 (amidohydrolase for N-terminal asparagine) as a magnetism responsive gene in rat brain. Ntan1 is an essential component of a protein degradation signal, which is a destabilizing N-terminal residue of a protein, in the N-end rule. In situ hybridization histochemistry revealed abundant expression of Ntan1 mRNA in hippocampal neurons in vivo. Northern blot analysis showed that Ntan1 mRNA was increased about three-fold after 3 h in response to brief magnetism. Brief magnetism also increased the transcriptional activity of Ntan1 promoter by luciferase reporter assay. Brief magnetism induced degradation of microtubule-associated protein 2 (MAP2) without affecting cell morphology and viability, which was prevented by a selective inhibitor of 26S proteasome in hippocampal neurons. Overexpression of Ntan1 using recombinant Ntan1 adenovirus vector resulted in a marked decrease in the MAP2 protein expression in hippocampal neurons. Our results suggest that brief magnetism leads to the induction of Ntan1 responsible for MAP2 protein degradation through ubiquitin–proteasome pathway in rat hippocampal neurons. [ABSTRACT FROM AUTHOR]

This paper presents the fundamental surge phenomenon when a step voltage is applied to a horizontal line above the ground which has branch feeders. We study line current and voltage at joint with the method of moments that calculates electromagnetic field in frequency domain. It is widely noted that the current reflection factor changes to a third at the junction of the same three lines in conventional transmission line theory based on TEM wave, and the stepwise current reflected appears overlapping to the incident current. On the other hand, the reflected current from the joint shows the inductive characteristic or the capacitive one depending on the angle between lines, which varies gradually and not stepwise in our numerical analysis considering TM wave. The time characteristic of the voltage at joint depends on the angles also, and the peak voltage slightly exceeds the voltage by the transmission line theory. These phenomena are caused by the electromagnetic coupling between the main line and branch lines. In addition to the computer simulations, we measured the currents in the reduced-scale model lines and the results show the validity of our analysis regarding changes of the reflected current depending on the angles. © 2003 Wiley Periodicals, Inc. Electr Eng Jpn, 144(2): 1–8, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.10176 [ABSTRACT FROM AUTHOR]

Rat cortical neurons cultured for 3 days in vitro were loaded with the fluorescent indicator fluo-3 for assessment of intracellular free calcium ion (Ca[sup 2+]) concentrations with the aid of a confocal laser-scanning microscope. In the absence of added MgCI[sub 2], the addition of NMDA induced a rapid but sustained increase in the number of fluorescent neurons in a concentration-dependent manner at a concentration range of 1-100 µM with the increase by KCI being transient. The addition of FeCI[sub 2], but not FeCI[sub 3], markedly inhibited the increase by NMDA in a reversible manner at concentrations of 10-200 µM, without affecting that by KCI. Extensive analyses revealed clear differentiation between inhibitions by ferrous iron and other channel blockers known to date. The inhibition by FeCI[sub 2] was completely prevented by the addition of two different iron chelators. Exposure to NMDA alone did not lead to cell death in immature cultured neurons, however, while further addition of FeCI[sub 2] invariably induced neuronal cell death 24 h after exposure. These results give support to our previous proposal that NMDA receptor complex may contain a novel site sensitive to blockade by ferrous iron in rat brain. [ABSTRACT FROM AUTHOR]