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Long non-coding RNAs (lncRNAs) have been shown to be aberrantly expressed in oral squamous cell carcinoma (OSCC), but the biological role and function of BRAF-activated long non-coding RNA (BANCR) in OSCC remain poorly understood. In this study, we found that the expression of BANCR was upregulated in OSCC tissues and cell lines compared to the negative control. The decreased expression of BANCR in vitro markedly inhibited OSCC cell proliferation, migration, and invasion while the opposite was observed for the overexpression of BANCR. The results also showed that the expression of MAPK signaling-related proteins (p-erk, p-akt, and p-p-38) was positively correlated with that of BANCR. Thus, BANCR may play an important role in the tumorigenesis of OSCC, as well as cell proliferation, migration, and invasion of OSCC, and it may be a potential therapeutic target and prognostic factor in OSCC patients. [ABSTRACT FROM AUTHOR]

Oral squamous cell carcinoma (OSCC) is one of the most common types of malignancies worldwide, and its morbidity and mortality have increased in the near term. Consequently, the purpose of the present study was to identify the notable differentially expressed genes (DEGs) involved in their pathogenesis to obtain new biomarkers or potential therapeutic targets for OSCC. The gene expression profiles of the microarray datasets GSE85195, GSE23558, and GSE10121 were obtained from the Gene Expression Omnibus (GEO) database. After screening the DEGs in each GEO dataset, 249 DEGs in OSCC tissues were obtained. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology pathway enrichment analysis was employed to explore the biological functions and pathways of the above DEGs. A protein–protein interaction network was constructed to obtain a central gene. The corresponding total survival information was analyzed in patients with oral cancer from The Cancer Genome Atlas (TCGA). A total of six candidate genes (CXCL10, OAS2, IFIT1, CCL5, LRRK2, and PLAUR) closely related to the survival rate of patients with oral cancer were identified, and expression verification and overall survival analysis of six genes were performed based on TCGA database. Time‐dependent receiver operating characteristic curve analysis yields predictive accuracy of the patient's overall survival. At the same time, the six genes were further verified by quantitative real‐time polymerase chain reaction using samples obtained from the patients recruited to the present study. In conclusion, the present study identified the prognostic signature of six genes in OSCC for the first time via comprehensive bioinformatics analysis, which could become potential prognostic markers for OCSS and may provide potential therapeutic targets for tumors. [ABSTRACT FROM AUTHOR]

Novel biphasic calcium phosphate (BCP) powders composed of α‐tricalcium phosphate (α‐TCP) and hydroxyapatite (HA) were prepared by thermal decomposition of carbonated amorphous calcium phosphates (CACP). At first, the CACP precipitates were synthesized by adding ammonium carbonate in the presence of poly(ethylene glycol) at pH 10 with an initial Ca/P molar ratio of 1.60 at 5°C. The Ca/P molar ratios of the CACP precursors are between 1.50 and 1.67 investigated by ICP. Then BCP (α‐TCP/HA) powders were obtained after heating the CACP precursors at relatively low temperature (800°C) for 3 h. α‐TCP/HA powders were characterized by X‐ray diffractometry, Fourier transform infrared spectra, transmission electron microscopy/scanning electron microscopy, and sedimentation experiment. The results show that α‐TCP and HA phases form in one powder, α‐TCP/HA powders are sphere with the diameter of 300 nm to less than 100 nm varied with their chemical compositions and the ratio of α‐TCP and HA in the powders can be adjusted by the adding amount of carbonates. The possible formation process of biphasic α‐TCP/HA powders was proposed. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009 [ABSTRACT FROM AUTHOR]