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Soil salinity leading to sodium toxicity is developing into a massive challenge for agricultural productivity globally, inducing osmotic, ionic, and redox imbalances in plants. Considering the predicted increase in salinization risk with the ongoing climate change, applying plant growth‐promoting rhizobacteria (PGPR) is an environmentally safe method for augmenting plant salinity tolerance. The present study examined the role of halotolerant Bacillus sp. BSE01 as a promising biostimulant for improving salt stress endurance in chickpea. Application of PGPR significantly increased the plant height, relative water content, and chlorophyll content of chickpea under both non‐stressed and salt stress conditions. The PGPR‐mediated tolerance towards salt stress was accomplished by the modulation of hormonal signaling and conservation of cellular ionic, osmotic, redox homeostasis. With salinity stress, the PGPR‐treated plants significantly increased the indole‐3‐acetic acid and gibberellic acid contents more than the non‐treated plants. Furthermore, the PGPR‐inoculated plants maintained lower 1‐aminocyclopropane‐1‐carboxylic acid and abscisic acid contents under salt treatment. The PGPR‐inoculated chickpea plants also exhibited a decreased NADPH oxidase activity with reduced production of reactive oxygen species compared to the non‐inoculated plants. Additionally, PGPR treatment led to increased antioxidant enzyme activities in chickpea under saline conditions, facilitating the reactive nitrogen and oxygen species detoxification, thereby limiting the nitro‐oxidative damage. Following salinity stress, enhanced K+/Na+ ratio and proline content were noted in the PGPR‐inoculated chickpea plants. Therefore, Bacillus sp. BSE01, being an effective PGPR and salinity stress reducer, can further be considered to develop a bioinoculant for sustainable chickpea production under saline environments. [ABSTRACT FROM AUTHOR]

Climate change negatively impacts the global hydrological resources leading to detrimental flood events. Submergence impedes the cellular membrane integrity, consequently affecting the membrane fluidity. Different abiotic stresses influence membrane lipid composition. Therefore, the remodeling of membrane lipids plays a major role in stress adaptation. Submergence‐induced membrane lipid peroxidation is well established in plants. However, dynamic changes in lipid composition for regulating submergence tolerance in rice remain so far unexplored. The present study explored the effect of submergence on the lipidomic profile of the Sub1 near‐isogenic lines (NILs) of rice, viz. Swarna, and Swarna Sub1 with contrasting submergence tolerance. The study also examined the association of lipidomic alteration with the membrane integrity and submergence tolerance. Submergence caused increased accumulation of reactive oxygen species (ROS), which was significantly higher in Swarna than Swarna Sub1. The lipid profile was also considerably altered under submergence. Following submergence, Swarna exhibited a significant decrease in phospholipid content accompanied by increased lipid peroxidation and electrolyte leakage. Furthermore, the disintegration of the thylakoid membrane resulted in a significant decrease in the chlorophyll content and photosynthesis rate under submergence. Submergence‐induced hypoxic condition also promoted starch depletion to fulfill the energy requirement. In contrast, submergence acclimation in Swarna Sub1 was associated with the shift to anaerobic respiration mediated by increased alcohol dehydrogenase (ADH) activity. Effective ROS detoxification in Swarna Sub1 facilitated by increased antioxidant enzyme activities contributed to the submergence tolerance by maintaining membrane integrity and photosynthetic activity. The present study established the direct association of lipid remodeling with membrane integrity, cell viability, and photosynthesis and also devised a crop model to reveal the molecular background of submergence tolerance in plants. [ABSTRACT FROM AUTHOR]

Drought stress induces the formation of cortical aerenchyma in roots, providing drought tolerance by reducing respiration. However, unrestricted aerenchyma formation impedes the radial transport of water through the root's central cylinder; thereby decreasing the water uptake under drought stress. Therefore, exploring the root architectural and anatomical alterations in rice under drought is essential for targeting crop improvement. Drought stress-induced accumulation of reactive oxygen species (ROS) plays a key role in the lysigenous aerenchyma development. However, the influence of nitric oxide (NO) and reactive nitrogen species (RNS) in the development of lysigenous aerenchyma under drought has never been studied in rice. The present study examined the effect of ROS and RNS, generated by progressive drought stress, on the lysigenous aerenchyma formation in the roots of contrasting rice genotypes of the Eastern Indo-Gangetic plains (EIGP). As expected, the PEG-induced drought stress stimulated the expression of NADPH oxidase (NOX), thereby promoting the ROS generation in roots of the rice seedlings. Excessive ROS and RNS accumulations in roots affected the membrane lipids, promoting the tissue-specific programmed cell death (PCD) in rice. The activation of the antioxidant defense system played a major role in the ROS and RNS detoxification, thereby restricting the root aerenchyma formation in rice under drought stress. The results also displayed that drought tolerance in rice is associated with the formation of the Casparian strip, which limits the apoplastic flow of water in the water-deficient roots. Overall, our study revealed the association of nitro-oxidative metabolism with PCD and lysigenous aerenchyma formation in the cortical cells of root under drought stress in rice. [ABSTRACT FROM AUTHOR]