Search

Background: Efforts to profile atopic dermatitis (AD) tissues have intensified, yet comprehensive analysis of systemic immune landscapes in severe AD remains crucial. Methods: Employing single‐cell RNA sequencing, we analyzed over 300,000 peripheral blood mononuclear cells from 12 severe AD patients (Eczema area and severity index (EASI) > 21) and six healthy controls. Results: Results revealed significant immune cell shifts in AD patients, including increased Th2 cell abundance, reduced NK cell clusters with compromised cytotoxicity, and correlated Type 2 innate lymphoid cell proportions with disease severity. Moreover, unique monocyte clusters reflecting activated innate immunity emerged in very severe AD (EASI > 30). While overall dendritic cells (DCs) counts decreased, a distinct Th2‐priming subset termed "Th2_DC" correlated strongly with disease severity, validated across skin tissue data, and flow cytometry with additional independent severe AD samples. Beyond the recognized role of Th2 adaptive immunity, our findings highlight significant innate immune cell alterations in severe AD, implicating their roles in disease pathogenesis and therapeutic potentials. Conclusion: Apart from the widely recognized role of Th2 adaptive immunity in AD pathogenesis, alterations in innate immune cells and impaired cytotoxic cells have also been observed in severe AD. The impact of these alterations on disease pathogenesis and the effectiveness of potential therapeutic targets requires further investigation. [ABSTRACT FROM AUTHOR]

An initial solution based on lattice reduction (LR) and a novel stopping criterion for the tabu search (TS) detection algorithm are proposed for use in multiple input multiple output (MIMO) antenna systems. LR aided‐detection is performed and its result is utilised as an initial solution to the TS algorithm, while a measure of the condition of the lattice‐reduced channel is used as a criterion for early termination of the algorithm. On the basis of the simulation results, this approach outperforms other initial solutions by about 3.5 dB at a BER of 10−3 for the 16‐QAM 16 × 16 MIMO with only a slight increment in computational cost. The proposed stopping criterion reduces the complexity further with virtually no loss in BER performance. [ABSTRACT FROM AUTHOR]