Search

Background: Dogs seized by law enforcement agencies during dogfighting investigations are at increased risk of Babesia gibsoni infection. A rapid and cost‐effective diagnostic test would increase the feasibility of mass screening of dogs for infection and monitoring treatment efficacy in B. gibsoni‐infected dogs. Objective: To determine the performance of a point‐of‐need insulated isothermal PCR (iiPCR) test for diagnosis of B. gibsoni in dogs rescued in dogfighting investigations. Animals: Two hundred and thirty‐three dogs seized in dogfighting investigations. Methods: Cross‐sectional study. Whole blood samples were tested for B. gibsoni and Babesia spp. by iiPCR. Results were compared to a reference standard comprised of concordant results from real‐time PCR in a commercial diagnostic laboratory and antibody titers. Results: The iiPCR system was quick to learn, portable, and had a short processing time of <2 hours. Sensitivity and specificity of the iiPCR assay for B. gibsoni were 90% (95% confidence interval [CI] 81–95%) and 99% (CI, 95–100%), respectively. Sensitivity and specificity of the iiPCR assay for Babesia spp. were 87% (CI, 78–93%) and 98% (CI, 0.94–99%), respectively. Conclusions and Clinical Importance: The iiPCR system produced few false‐positive results, indicating that positive results are likely to represent true infections when used in high‐risk animals. The iiPCR system can fail to identify 10–15% of truly infected dogs. However, the portability, speed, and economy of the iiPCR system compared to testing through a reference laboratory can allow rescue groups to screen and identify infection in more dogs. [ABSTRACT FROM AUTHOR]

The article describes the case of a cat with idiopathic CD4+ T lymphocytopenia infected with chronic disseminated Mycobacterium xenopi. Condition of the cat upon examination is described. The diagnosis was made based on the finding of acid-fast bacilli in multiple organs. A background on disseminated mycobcteriosis is offered. In this case, a survival time of approximately 7 years was achieved through the use of enrofloxacin or ciprofloxacin, rifampin, clofazimine and clarithromycin.

Background: Serodiagnosis of feline immunodeficiency virus (FIV) is complicated by the use of a formalin-inactivated whole-virus FIV vaccine. Cats respond to immunization with antibodies indistinguishable from those produced during natural infection by currently available diagnostic tests, which are unable to distinguish cats that are vaccinated against FIV, infected with FIV, or both. Hypothesis: An enzyme-linked immunosorbent assay (ELISA) detecting antibodies against formalin-treated FIV whole virus and untreated transmembrane peptide will distinguish uninfected from infected cats, regardless of vaccination status. Animals: Blood samples were evaluated from uninfected unvaccinated cats (n = 73 samples), uninfected FIV-vaccinated cats (n = 89), and FIV-infected cats (n = 102, including 3 from cats that were also vaccinated). Methods: The true status of each sample was determined by virus isolation. Plasma samples were tested for FIV antibodies by a commercial FIV diagnostic assay and an experimental discriminant ELISA. Results: All samples from uninfected cats were correctly identified by the discriminant ELISA (specificity 100%). Of the samples collected from FIV-infected cats, 99 were correctly identified as FIV-infected (sensitivity 97.1%). Conclusions and Clinical Importance: With the exception of viral isolation, the discriminant ELISA is the most reliable assay for diagnosis of FIV. A practical strategy for the diagnosis of FIV infection would be to use existing commercial FIV antibody assays as screening tests. Negative results with commercial assays are highly reliable predictors for lack of infection. Positive results can be confirmed with the discriminant ELISA. If the discriminant ELISA is negative, the cat is probably vaccinated against FIV but not infected. Positive results are likely to represent infection. [ABSTRACT FROM AUTHOR]

Background: Vaccination and importation of dogs and cats are prohibited in the Galapagos, resulting in a uniquely isolated population. The purpose of this study was to determine the prevalence of infectious diseases of dogs and cats that impact their health, could spill over to native wildlife, or sentinel diseases of concern to humans. Hypothesis: The isolation of dogs and cats in the Galapagos protects them from diseases common in mainland populations. Animals: Ninety-five dogs and 52 cats presented during a neutering campaign. Methods: A prospective cross-sectional study was performed. Blood was collected for serological and DNA evaluation of a panel of infectious diseases. Results: Antibodies against parvovirus (100%), parainfluenza virus (100%), adenovirus 1/2 (66–67%), and distemper virus (22%) were present in dogs. Dirofilaria immitis was also common in dogs (34%), with lower prevalences of Wolbachia pipiens (22%), Bartonella sp. (13%), Ehrlichia/ Anaplasma spp. (1%), and Mycoplasma haemocanis (1%) observed. Antibodies against panleukopenia virus (67%), Toxoplasma gondii (63%), calicivirus (44%), and herpesvirus 1 (10%) were detected in cats. Feline leukemia virus antigen, feline immunodeficiency virus antibody, or coronavirus antibodies were not detected. Bartonella sp. (44%) infections were common in cats, but only one was infected with M. haemofelis. Conclusions and Clinical Importance: Despite their relative seclusion from the rest of the world, cats and dogs of Isabela were exposed to many pathogens found in mainland South America. Parasite prophylaxis, neutering, and strict enforcement of animal movement restrictions would control a majority of the diseases. In the absence of vaccination, a reservoir of susceptible animals remains vulnerable to new disease introductions. [ABSTRACT FROM AUTHOR]

An intrathoracic mass was discovered as an incidental finding in a 14-year-old, spayed, female Rottweiler cross during evaluation of urinary incontinence. Computed tomography suggested a pericardial or pleural location and high adipose content of the mass. The mass was removed via lateral thoracotomy with partial pericardectomy and was diagnosed as a pericardial lipoma. The dog recovered well, and there was no evidence of recurrence approximately one year later. Adipose tumours of the heart and its associated structures are rare in dogs and have been associated with both successful and fatal outcomes. [ABSTRACT FROM AUTHOR]

Upper respiratory infection (URI) is an important cause of morbidity and mortality in shelter cats. Malnutrition, specifically vitamin A deficiency, has been associated with respiratory tract infections in humans in developing countries. The purpose of this study was to determine whether feral cats and cats in shelters have lower serum vitamin A concentrations than pet cats, because it was surmised that a vitamin A deficiency may predispose these cats to develop URI. This was a cross-sectional study in which serum retinol, α-tocopherol, γ-tocopherol and beta carotene concentrations were measured from 48 shelter cats, 56 feral cats, and 42 pet cats, using HPLC. Retinol, α-tocopherol, and γ-tocopherol concentrations were found to be significantly lower in the shelter and feral cat populations than in pet cats (p ≤ 0.0001). There was no evidence of an effect of sex or neuter status on vitamin concentrations. Sick feral cats had a higher beta carotene level than did healthy feral cats. Within shelter and feral populations, no other effect of health status was found. The lower mean serum retinol and tocopherol concentrations in the feral and shelter cat groups could be due to oxidative or other forms of stress, the amount of vitamin consumed, or combinations of these. Further studies are needed to determine whether supplementation of fat-soluble vitamins improves the health status of feral or shelter cats. [ABSTRACT FROM AUTHOR]