1. Self‐Immolative Activation of β‐Galactosidase‐Responsive Probes for In Vivo MR Imaging in Mouse Models.
- Author
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Lilley, Laura M., Kamper, Sarah, Caldwell, Michael, Chia, Zer Keen, Ballweg, David, Vistain, Luke, Krimmel, Jeffrey, Mills, Teresa Anne, MacRenaris, Keith, Lee, Paul, Waters, Emily Alexandria, and Meade, Thomas J.
- Subjects
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MAGNETIC resonance imaging , *GALACTOSIDASES , *STRUCTURAL isomers , *MICE , *CARBOXYLATES , *STRUCTURAL design - Abstract
Our lab has developed a new series of self‐immolative MR agents for the rapid detection of enzyme activity in mouse models expressing β‐galactosidase (β‐gal). We investigated two molecular architectures to create agents that detect β‐gal activity by modulating the coordination of water to GdIII. The first is an intermolecular approach, wherein we designed several structural isomers to maximize coordination of endogenous carbonate ions. The second involves an intramolecular mechanism for q modulation. We incorporated a pendant coordinating carboxylate ligand with a 2, 4, 6, or 8 carbon linker to saturate ligand coordination to the GdIII ion. This renders the agent ineffective. We show that one agent in particular (6‐C pendant carboxylate) is an extremely effective MR reporter for the detection of enzyme activity in a mouse model expressing β‐gal. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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