Search

Both seed germination and subsequent seedling establishment are key checkpoints during the life cycle of seed plants, yet flooding stress markedly inhibits both processes, leading to economic losses from agricultural production. Here, we report that melatonin (MT) seed priming treatment enhances the performance of seeds from several crops, including soybean, wheat, maize, and alfalfa, under flooding stress. Transcriptome analysis revealed that MT priming promotes seed germination and seedling establishment associated with changes in abscisic acid (ABA), gibberellin (GA), and reactive oxygen species (ROS) biosynthesis and signaling pathways. Real‐time quantitative RT‐PCR (qRT‐PCR) analysis confirmed that MT priming increases the expression levels of GA biosynthesis genes, ABA catabolism genes, and ROS biosynthesis genes while decreasing the expression of positive ABA regulatory genes. Further, measurements of ABA and GA concentrations are consistent with these trends. Following MT priming, quantification of ROS metabolism‐related enzyme activities and the concentrations of H2O2 and superoxide anions (O2−) after MT priming were consistent with the results of transcriptome analysis and qRT‐PCR. Finally, exogenous application of GA, fluridone (an ABA biosynthesis inhibitor), or H2O2 partially rescued the poor germination of non‐primed seeds under flooding stress. Collectively, this study uncovers the application and molecular mechanisms underlying MT priming in modulating crop seed vigor under flooding stress. [ABSTRACT FROM AUTHOR]

Transcriptional regulation plays a key role in the control of seed dormancy, and many transcription factors (TFs) have been documented. However, the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely unknown. Here, we showed that TF PHYTOCHROME‐INTERACTING FACTOR4 (PIF4) physically interacted with the abscisic acid (ABA) signaling responsive TF ABSCISIC ACID INSENSITIVE4 (ABI4) to act as a transcriptional complex to promote ABA biosynthesis and signaling, finally deepening primary seed dormancy. Both pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype, with a synergistic effect in the pif4/abi4 double mutant. PIF4 binds to ABI4 to form a heterodimer, and ABI4 stabilizes PIF4 at the protein level, whereas PIF4 does not affect the protein stabilization of ABI4. Subsequently, both TFs independently and synergistically promoted the expression of ABI4 and NCED6, a key gene for ABA anabolism. The genetic evidence is also consistent with the phenotypic, physiological and biochemical analysis results. Altogether, this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling. [ABSTRACT FROM AUTHOR]

Narrowband emissive multiple resonance (MR) emitters promise high efficiency and stability in deep‐blue organic light‐emitting diodes (OLEDs). However, the construction of ideal ultra‐narrow‐band deep‐blue MR emitters still faces formidable challenges, especially in balancing bathochromic‐shift emission, spectral narrowing, and aggregation suppression. Here, DICz is chosen, which possesses the smallest full‐width‐at‐half‐maximum (FWHM) in MR structures, as the core and solved the above issue by tuning its peripheral substitution sites. The 1‐substituted molecule Cz‐DICz is able to show a bright deep‐blue emission with a peak at 457 nm, an extremely small FWHM of 14 nm, and a CIE coordinate of (0.14, 0.08) in solution. The corresponding OLEDs exhibit high maximum external quantum efficiencies of 22.1%–25.6% and identical small FWHMs of 18 nm over the practical mass‐production concentration range (1–4 wt.%). To the best of the knowledge, 14 and 18 nm are currently the smallest FWHM values for deep‐blue MR emitters with similar emission maxima under photoluminescence and electroluminescence conditions, respectively. These discoveries will help drive the development of high‐performance narrowband deep‐blue emitters and bring about a revolution in OLED industry. [ABSTRACT FROM AUTHOR]

Summary: Abscisic acid (ABA) and gibberellins (GA) antagonistically mediate several biological processes, including seed germination, but the molecular mechanisms underlying ABA/GA antagonism need further investigation, particularly any role mediated by a transcription factors module.Here, we report that the DELLA protein RGL2, a repressor of GA signaling, specifically interacts with ABI4, an ABA signaling enhancer, to act as a transcription factor complex to mediate ABA/GA antagonism.The rgl2, abi3, abi4 and abi5 mutants rescue the non‐germination phenotype of the ga1‐t. Further, we demonstrate that RGL2 specifically interacts with ABI4 to form a heterodimer. RGL2 and ABI4 stabilize one another, and GA increases the ABI4‐RGL2 module turnover, whereas ABA decreases it. At the transcriptional level, ABI4 enhances the RGL2 expression by directly binding to its promoter via the CCAC cis‐element, and RGL2 significantly upregulates the transcriptional activation ability of ABI4 toward its target genes, including ABI5 and RGL2. Abscisic acid promotes whereas GA inhibits the ability of ABI4‐RGL2 module to activate transcription, and ultimately ABA and GA antagonize each other. Genetic analysis demonstrated that both ABI4 and RGL2 are essential for the activity of this transcription factor module.These results suggest that the ABI4‐RGL2 module mediates ABA/GA antagonism by functioning as a double agent. [ABSTRACT FROM AUTHOR]

BACKGROUND: Although a great deal of research has examined the impact of socialized pest control service, few studies have discussed the relationship between socialized pest control service and pesticide use intensity. In particular, the literature ignores the impact of advanced application technologies (drone sprayers) on the intensity of pesticide use by farmers. RESULTS: Based on a survey of 1185 rice growers in Hubei Province, China, this study found that 64.13% of the sample farmers used pesticides more than three times in one rice season. Importantly, socialized pest control services have a significant negative effect on the pesticide use intensity of farmers. Compared to the sample that did not purchase the service, farmers who purchased socialized pest control services demonstrated 9.30% less pesticide intensity. Further, there was a significant difference among farmers using different application devices on pesticide use intensity. Compared to the sample using ground backpack sprayers, farmers using drone sprayers used 12.40% less pesticide intensity. CONCLUSION: This study found that the frequency of pesticide use by farmers during rice cultivation was generally high. Socialized pest control services have played a positive role in reducing the intensity of pesticide use, especially with the more obvious pesticide reduction effect of the adoption of drone sprayers. Therefore, improving socialized pest control services and promoting advanced equipment such as drone sprayers may be an important way to reduce the use of pesticides in China. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

BACKGROUND: Although scholars have elaborated on the effects of aging, there is no consensus on the relationship between the aging and farmers' pesticide input. Regional differences in agricultural crops in China, namely 'Southern Rice and Northern Wheat', have not been considered in any of the existing studies. RESULTS: Based on the data of 443 wheat farmers and 324 rice farmers in China, this study found that aging significantly increased pesticide input. After sorting out the types of pesticides, it was found that the aging significantly increased the input of biological pesticides, but did not affect the input of chemical pesticides. After considering the planting pattern of 'Southern Rice and Northern Wheat', aging did not have a significant impact on the pesticide input of wheat farmers, but had a significant positive impact on the pesticide input of rice farmers. For wheat farmers in the north, social services have a significant inhibitory effect on the relationship between aging and pesticide input. For rice farmers, path dependence has a significant role in promoting the relationship between aging and pesticide input. CONCLUSION: The planting pattern of 'Southern Rice and Northern Wheat' will indeed affect the relationship between aging and pesticide input, and social services and path dependence are the main reasons for this heterogeneity effect. Therefore, improving agricultural socialization services and reducing farmers' dependence on traditional production experience will be an important way to effectively reduce the impact of aging. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Aim: To assess the effect of lubricants on reducing perineal trauma during vaginal delivery. Methods: PubMed, Embase, the Cochrane Library, CINAHL, China National Knowledge Infrastructure, China Biology Medicine disc, WanFang databases, and ClinicalTrials.gov, were searched for literature up to 25 June 2021. Randomized controlled trials published in English or Chinese that compared the vaginal application of lubricant with standard care for women were included. Two reviewers independently performed study screening, data extraction, risk of bias assessment, and certainty of evidence assessment. Pooled effect sizes and corresponding 95% confidence intervals (CI) were calculated using meta‐analysis. Results: Nineteen trials enrolling 5445 pregnant women were included. Compared with standard care, women using lubricants had a lower incidence of perineal trauma (risk ratio [RR] 0.84, 95% CI 0.76–0.93; low certainty evidence), second‐degree perineal laceration (RR 0.72, 95% CI 0.64–0.82; moderate certainty evidence) and episiotomy (RR 0.77, 95% CI 0.62–0.96; very low certainty evidence), and had a shorter duration of the second‐stage labor (MD −13.72 min, 95% CI −22.68 to −4.77; very low certainty evidence). Conclusion: Lubricants might reduce the incidence of perineal trauma, especially second‐degree perineal laceration, and shorten the duration of the second‐stage labor. More well‐designed studies will continue developing high‐quality evidence in this field. [ABSTRACT FROM AUTHOR]

BACKGROUND: Although scholars have become increasingly concerned about finding ways to encourage more farmers to use biopesticides and fewer to use chemical pesticides, few studies have investigated the prevalence of Chinese rice farmers' mixed use of biopesticides and chemical pesticides for pest management. RESULTS: Based on a household survey in Hubei Province, China, this study found that 59.18% of the sample rice farmers used a mix of biopesticides and chemical pesticides. There is mainly a complementarity relationship between rice farmers' decision to use biopesticides and chemical pesticides. The pursuit of multiple production purposes is the main reason why farmers decide on the mixed‐use of pesticides. Specifically, farmers generally tend to use biopesticides to ensure food safety for consumption, and chemical pesticides to reduce costs for profitability. The decision of farmers to mix pesticides has led to an increase of pesticide varieties and costs. However, it reduces the frequency of pesticide use, and does not result in a significant increase in rice yield and output value. CONCLUSION: While food safety should be emphasized in working towards greater use of biopesticides, rice farmers' mixed use of biopesticides and chemical pesticides will remain for a long time until the biotechnology is more developed. In fact, programs that investigate the effects of a combination of biopesticides and chemical pesticides should be implemented to achieve pesticide reduction, because biopesticides and chemical pesticides have specific disadvantages. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Killer cell immunoglobulin‐like receptor (KIR) receptor‐ligand mismatch has been shown to be protective for acute and chronic graft‐versus‐host disease (aGVHD, cGVHD) following allogeneic hematopoietic stem cell transplantation (allo‐HSCT) for acute leukemia. Mesenchymal stem cells (MSC) have been considered as one of the most promising prophylaxis for severe GVHD. However, there are no prospective or retrospective studies determining whether they can work synergistically on GVHD. To investigate the potential influence of KIR matching and MSCs, and their synergism on aGVHD and cGVHD after allo‐HSCT in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) patients. Data from 104 patients with AML and 50 patients with ALL treated with allo‐HSCT in the transplantation unit were retrospectively analyzed. KIR genotyping was performed by the PCR‐SSO method. The amplicons were quantified on the Luminex 200 flow analyzer and analyzed using the Quick‐Type for Lifecodes software to generate KIR data. Cox proportional hazards models were used in multivariate analyses. KIR receptor‐ligand matching was associated with an increased risk of grade II–IV aGVHD compared to KIR receptor‐ligand mismatching (p < 0.001) in AML patients, but KIR ligand‐mismatching had no significant effect on aGVHD or cGVHD in ALL patients. In contrast, MSCs reduced the incidence of grade II–IV aGVHD in both AML and ALL patients (AML: p = 0.006; ALL: p = 0.008) regardless of KIR mismatching. The combination of KIR receptor‐ligand mismatch and MSC transplantation significantly suppressed grade II–IV aGVHD occurrence in AML patients (p = 0.039). In the KIR mismatch group, the incidence of aGVHD was 2.8% in patients receiving MSC compared to 14.6% in those who did not (p = 0.047). KIR receptor‐ligand mismatch, MSC transplantation and their combined use significantly reduced the risk of aGVHD after allo‐HSCT. These data provide a clinically applicable strategy to reduce aGVHD, thus improving allo‐HSCT outcome. [ABSTRACT FROM AUTHOR]

Hepatocellular carcinoma (HCC) is one of the most common public health challenges, worldwide. Because of molecular complexity and tumor heterogeneity, there are no effective predictive models for prognosis of HCC. This underlines the unmet need for accurate prognostic models for HCC. Analysis of GSE14520 data from gene omnibus (GEO) database identified multiple differentially expressed mRNAs (DEMs) between HCC and normal tissues. After randomly stratifying the patients into the training and testing groups, we performed univariate, lasso, and multivariable Cox regression analyses to delineate the prognostic gene signature in training set. We then used Kaplan–Meier plot, time‐dependent receiver operating characteristic (ROC), multivariable Cox regression analysis of clinical information, nomogram, and decision curve analysis (DCA) to evaluate the predictive and overall survival value of a novel five‐gene signature (CNIH4, SOX4, SPP1, SORBS2, and CCL19) within and across sets, separately and combined. We also validated the prognostic value of the five‐gene signature using The Cancer Genome Atlas—Liver Hepatocellular Carcinoma (TCGA‐LIHC), GSE54236 and International Cancer Genome Consortium (ICGC) sets. Multivariable Cox regression analysis revealed that the five‐gene signature and tumor node metastasis (TNM) stage were independent prognostic factors for overall survival of HCC patients in GSE14520 and TCGA‐LIHC. Combining TNM stage clinical pathological parameters and nomogram greatly improved the prognosis prediction of HCC. Further gene set enrichment analysis (GSEA) revealed enrichment of KEGG pathways related to cell cycle in the high‐risk group and histidine metabolism in the low‐risk group. Finally, all these five mRNAs are overexpressed between 12 pairs of HCC and adjacent normal tissues by quantitative real‐time PCR validation. In brief, a five‐gene prognostic signature and a nomogram were identified and constructed, respectively, and further validated for their HCC prognostic value. The five‐gene risk score together with TNM stage models could aid in rationalizing customized therapies in HCC patients. [ABSTRACT FROM AUTHOR]

Summary: Salinity stress enhances reactive oxygen species (ROS) accumulation by activating the transcription of NADPH oxidase genes such as RbohD, thus mediating plant developmental processes, including seed germination. However, how salinity triggers the expression of ROS‐metabolism‐related genes and represses seed germination has not yet been fully addressed.In this study, we show that Abscisic Acid‐Insensitive 4 (ABI4), a key component in abscisic acid (ABA) signaling, directly combines with RbohD and Vitamin C Defective 2 (VTC2), the key genes involved in ROS production and scavenging, to modulate ROS metabolism during seed germination under salinity stress.Salinity‐induced ABI4 enhances RbohD expression by physically interacting with its promoter, and subsequently promotes ROS accumulation, thus resulting in cell membrane damage and a decrease in seed vigor. Additional genetic evidence indicated that the rbohd mutant largely rescues the salt‐hypersensitive phenotype of ABI4 overexpression seeds. Consistently, the abi4/vtc2 double mutant showed the salt‐sensitive phenotype, similar to the vtc2 mutant, suggesting that both RbohD and VTC2 are epistatic to ABI4 genetically.Altogether, these results suggest that the salt‐induced RbohD transcription and ROS accumulation is dependent on ABI4, and that the ABI4‐RbohD/VTC2 regulatory module integrates both ROS metabolism and cell membrane integrity, ultimately repressing seed germination under salinity stress. [ABSTRACT FROM AUTHOR]

Aim: This study aimed to investigate changes in skin temperature in the main body regions of ovariectomized rats under different incubation temperatures to identify regions that are similar to hot flashes experienced by menopausal women. Methods: A total of 96 adult female Sprague–Dawley rats were randomly divided into sham, ovariectomized and ovariectomized with estrogen treatment groups, with treatment lasting for 4 weeks. After 3 weeks of treatment, each group was randomly divided into five subgroups and placed in separate incubators set at 4, 15, 25, and 37°C. Changes in the skin temperature in seven main regions (head, neck, chest, abdomen, back, tail, and paws) for four time intervals (0–3 min, 3–5 min, 5–10 min and 10–15 min) were monitored using infrared thermography. Results: All rats showed rapid changes in skin temperature followed by a gradual slowdown under different incubation temperatures. However, changes in ovariectomized rats were significantly different from that in normal rats, and changes on the back, tail and paws were more rapid and lasted longer. Estrogen treatment effectively controlled these abnormalities of ovariectomized rats. Conclusions: Temperature responses in the back, tail and paws in ovariectomized rats might be similar to the face, neck and upper chest in menopausal women, where the symptoms of hot flashes are most obvious, which suggests that the back, tail and paws could be regarded as the focus of research on hot flashes, and offer theoretical foundations for mechanisms behind the occurrence of hot flashes in specific regions. Geriatr Gerontol Int 2020; ••: ••–••. [ABSTRACT FROM AUTHOR]

Both seed germination and early seedling establishment are important biological processes in a plant's lifecycle. Seed longevity is a key trait in agriculture, which directly influences seed germination and ultimately determines crop productivity and hence food security. Numerous studies have demonstrated that seed deterioration is regulated by complex interactions between diverse endogenous genetically controlled factors and exogenous environmental cues, including temperature, relative humidity, and oxygen partial pressure during seed storage. The endogenous factors, including the chlorophyll concentration, the structure of the seed coat, the balance of phytohormones, the concentration of reactive oxygen species, the integrity of nucleic acids and proteins and their associated repair systems, are also involved in the control of seed longevity. A precise understanding of the regulatory mechanisms underlying seed longevity is becoming a hot topic in plant molecular biology. In this review, we describe recent research into the regulation of seed longevity and the interactions between the various environmental and genetic factors. Based on this, the current state‐of‐play regarding seed longevity regulatory networks will be presented, particularly with respect to agricultural seed storage, and the research challenges to be faced in the future will be discussed. This review primarily discussed the underlying regulatory mechanisms of seed longevity from molecular, physiological and environmental aspects, including seed coat structure, phytohormones, energy storage and metabolism cascades. [ABSTRACT FROM AUTHOR]

Using survey data from 280 farmers in Jianghan Plain, China, this paper establishes an evaluation index system for three dimensions of farmers' flood perceptions and then uses the entropy method to estimate their overall flood perception. Farmers' flood perceptions exhibit the following characteristics: (i) their flood-occurrence, flood-prevention, and overall flood perceptions gradually increase with age, whereas their flood-effects perception gradually decreases; (ii) their flood-occurrence and flood-effects perceptions gradually increase with a higher level of education, whereas their flood-prevention perception gradually decreases and their overall flood perception shows nonlinear change; (iii) flood-occurrence, flood-effects, and overall flood perceptions are higher among farmers who serve in public offices than among those who do not do so; (iv) the flood-occurrence, flood-effects, and overall flood perceptions of farmers who work off-farm are higher than those of farmers who work solely on-farm, contrary to the flood-prevention perception; and (v) the flood-effects and flood-prevention perceptions of male farmers are lower than those of female farmers, but the flood-occurrence and overall flood perceptions of male farmers are higher than those of female farmers. [ABSTRACT FROM AUTHOR]