22 results on '"Nishikomori, Ryuta"'
Search Results
2. Role of the NOD2 Genotype in the Clinical Phenotype of Blau Syndrome and Early-Onset Sarcoidosis.
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Okafuji, Ikuo, Nishikomori, Ryuta, Kanazawa, Nobuo, Kambe, Naotomo, Fujisawa, Akihiro, Yamazaki, Shin, Saito, Megumu, Yoshioka, Takakazu, Kawai, Tomoki, Sakai, Hidemasa, Tanizaki, Hideaki, Heike, Toshio, Miyachi, Yoshiki, and Nakahata, Tatsutoshi
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GENETIC disorders , *NUCLEOTIDES , *SARCOIDOSIS , *LYMPHOPROLIFERATIVE disorders , *MEDICAL research , *THERAPEUTICS - Abstract
The article presents a study regarding the role of the nucleotide-binding oligomerization domain 2 gene (NOD2 gene) in the clinical phenotype of Blau syndrome and early-onset sarcoidosis (EOS). The purpose of the study is to identify an association between the clinical phenotype of Blau syndrome and EOS and NOD2 and its associate basal NF-kB activity. Meanwhile, the result of the study suggests that NOD2 gene may help indicate disease progression in patient with Blau syndrome and EOS.
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- 2009
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3. Systemic inflammation caused by an intracranial mesenchymal tumor with a EWSR1::CREM fusion presenting associated with IL‐6/STAT3 signaling.
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Hojo, Keishiro, Furuta, Takuya, Komaki, Satoru, Yoshikane, Yukako, Kikuchi, Jin, Nakamura, Hideo, Ide, Mizuki, Shima, Saho, Hiyoshi, Yusuke, Araki, Junichiro, Tanaka, Seiji, Ozono, Shuichi, Yoshida, Akihiko, Nobusawa, Sumihito, Morioka, Motohiro, and Nishikomori, Ryuta
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INTRACRANIAL tumors , *FEVER , *CEREBROSPINAL fluid examination , *CASTLEMAN'S disease , *BRAIN tumors , *ARACHNOID cysts ,BONE marrow examination - Abstract
Pediatric neoplastic diseases account for about 10% of cases of fever of unknown origin (FUO), and most neoplastic disease cases are leukemia, lymphoma, and neuroblastoma. Brain tumors are rarely reported as the cause of FUO, although craniopharyngioma, metastatic brain tumor, and Castleman's disease have been reported. We report a case of intracranial mesenchymal tumor (IMT) with a FET:CREB fusion gene, which had inflammatory phenotype without neurological signs. A 10‐year‐old girl was admitted with a 2‐month history of intermittent fever and headache, whereas her past history as well as her family history lacked special events. Sepsis work‐up showed no pathological organism, and empirical antibiotic therapy was not effective. Bone marrow examination showed a negative result. Cerebrospinal fluid examination showed elevated protein as well as cell counts, and head magnaetic resonance imaging showed a hypervascular mass lesion with contrast enhancement in the left cerebellar hemisphere. The patient underwent tumor excision, which made the intermittent fever disappear. Pathological examinations resembled those of classic angiomatoid fibrous histiocytoma (AFH), but the morphological features were distinct from the AFH myxoid variant; then we performed break‐apart fluorescence in situ hybridization and confirmed the tumor harbored the rare EWSR1::CREM fusion gene (Ewing sarcoma breakpoint region 1 gene (EWSR1) and cAMP response element binding (CREB) family gene). Consequently, we diagnosed the condition as IMT with EWSR1::CREM fusion. Elevated serum concentration of interleukin 6 (IL‐6) was normalized after tumor resection, which suggested the fever could be caused by tumor‐derived IL‐6. This is the first case of IMT with EWSR1::CREM fusion that showed paraneoplastic symptoms associated with the IL‐6/signal transducer and activator of transcription 3 (STAT3) signaling pathway. Although brain tumors are rarely diagnosed as a responsible disease for FUO, they should be considered as a cause of unknown fever even in the absence of abnormal neurological findings. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Novel AP3B1 mutations in a Hermansky–Pudlak syndrome type2 with neonatal interstitial lung disease.
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Matsuyuki, Keigo, Ide, Mizuki, Houjou, Keishirou, Shima, Saho, Tanaka, Seiji, Watanabe, Yoriko, Tomino, Hiroyuki, Egashira, Tomoko, Takayanagi, Toshimitsu, Tashiro, Katsuya, Okamura, Ken, Suzuki, Tamio, Miyamoto, Takayuki, Shibata, Hirofumi, Yasumi, Takahiro, and Nishikomori, Ryuta
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- 2022
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5. Augmentation of Stimulator of Interferon Genes–Induced Type I Interferon Production in COPA Syndrome.
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Kato, Takashi, Yamamoto, Masaki, Honda, Yoshitaka, Orimo, Takashi, Sasaki, Izumi, Murakami, Kohei, Hemmi, Hiroaki, Fukuda‐Ohta, Yuri, Isono, Kyoichi, Takayama, Saki, Nakamura, Hidenori, Otsuki, Yoshiro, Miyamoto, Toshiaki, Takita, Junko, Yasumi, Takahiro, Nishikomori, Ryuta, Matsubayashi, Tadashi, Izawa, Kazushi, and Kaisho, Tsuneyasu
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IN vitro studies , *GENETIC mutation , *GENETICS , *JOINT diseases , *ANIMAL experimentation , *INTERSTITIAL lung diseases , *PERIPHERAL nervous system , *INTERFERONS , *KIDNEY diseases , *GENE expression , *GENOMES , *DESCRIPTIVE statistics , *MICE - Abstract
Objective: Coatomer subunit alpha (COPA) syndrome, also known as autoinflammatory interstitial lung, joint, and kidney disease, is caused by heterozygous mutations in COPA. We identified a novel COPA variant in 4 patients in one family. We undertook this study to elucidate whether and how the variant causes manifestations of COPA syndrome by studying these 4 patients and by analyzing results from a gene‐targeted mouse model. Methods: We performed whole‐exome sequencing in 7 family members and measured the type I interferon (IFN) signature of the peripheral blood cells. We analyzed the effects of COPA variants in in vitro experiments and in Copa mutant mice that were generated. Results: We identified a heterozygous variant of COPA (c.725T>G, p.Val242Gly) in the 4 affected members of the family. The IFN score was high in the members carrying the variant. In vitro analysis revealed that COPA V242G, as well as the previously reported disease‐causing variants, augmented stimulator of interferon genes (STING)–induced type I IFN promoter activities. CopaV242G/+ mice manifested interstitial lung disease and STING‐dependent elevation of IFN‐stimulated gene expression. In CopaV242G/+ dendritic cells, the STING pathway was not constitutively activated but was hyperactivated upon stimulation, leading to increased type I IFN production. Conclusion: V242G, a novel COPA variant, was found in 4 patients from one family. In gene‐targeted mice with the V242G variant, interstitial lung disease was recapitulated and augmented responses of the STING pathway, leading to an increase in type I IFN production, were demonstrated. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Breastfeeding and the prevalence of allergic diseases in schoolchildren: Does reverse causation matter?
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Kusunoki, Takashi, Morimoto, Takeshi, Nishikomori, Ryuta, Yasumi, Takahiro, Heike, Toshio, Mukaida, Kumiko, Fujii, Tatsuya, and Nakahata, Tatsutoshi
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BREASTFEEDING , *ALLERGIES , *INFANTS , *ATOPY , *SURVEYS - Abstract
Kusunoki T, Morimoto T, Nishikomori R, Yasumi T, Heike T, Mukaida K, Fujii T, Nakahata T. Breastfeeding and the prevalence of allergic diseases in schoolchildren: Does reverse causation matter? Pediatr Allergy Immunol 2010: 21: 60–66. © 2010 John Wiley & Sons A/S Infants at higher risk of allergic diseases might be breastfed for longer periods compared with infants at lower risk in the hope that breastfeeding might reduce the risk of atopic disorders. Therefore, this intention could manifest as an apparent allergy-promoting effect of breastfeeding or reverse causation. To analyze the effect of breast feeding on the prevalence of allergic diseases at school age, a large questionnaire survey was administered to the parents of schoolchildren aged 7–15 yrs. 13,215 parents responded (response rate, 90.1%). Prevalence rates of allergic diseases were compared according to the type of feeding in infancy (either complete breastfeeding, mixed feeding or complete artificial feeding). In both univariate and multivariate analysis, compared with those with complete artificial feeding, those with mixed and complete breastfeeding showed a significantly lower prevalence of bronchial asthma (BA) (p = 0.01 and 0.003, respectively). On the other hand, in univariate analysis, the prevalence of atopic dermatitis (AD) and food allergy (FA) were significantly higher in those with complete breastfeeding (p = 0.04 and 0.01, respectively). There was a significantly higher proportion of complete breastfeeding among those with greater risk of allergic diseases (presence of family history, either eczema or wheeze within 6 months after birth, or FA in infancy). Therefore, our multivariate analysis included these risks as confounding factors, and we found that the promoting effects of breastfeeding on AD and FA disappeared. In conclusion, our data clearly showed the inhibitory effect of breastfeeding on the prevalence of BA at school age. The apparent promoting effect of breastfeeding on the prevalence of AD and FA is most likely because of reverse causation. [ABSTRACT FROM AUTHOR]
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- 2010
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7. Allergic status of schoolchildren with food allergy to eggs, milk or wheat in infancy.
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Kusunoki, Takashi, Morimoto, Takeshi, Nishikomori, Ryuta, Heike, Toshio, Fujii, Tatsuya, and Nakahata, Tatsutoshi
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FOOD allergy in children , *ALLERGY in children , *INFANTS , *ASTHMA , *ATOPIC dermatitis - Abstract
Although children allergic to eggs, milk or wheat in infancy tend to become tolerant by school age, the allergic status of these children at school age has not been well evaluated. To investigate the allergic status of schoolchildren who avoided eggs, milk or wheat because of an immediate-type allergic reaction at <1-yr-old (food avoiders in infancy), we conducted a large-scale questionnaire-based survey of schoolchildren. A questionnaire on allergic diseases was distributed to the parents of 14,669 schoolchildren aged 7 to 15 yr in 30 schools in Kyoto, Japan. Of these, 13,215 responded (response rate, 90.1%). The rate of 7-yr-old children who were food avoiders in infancy was 5.4%. This rate decreased as the current age of the children increased, down to 3% in 15-yr-old children, indicating that food allergy in infancy tended to become more prevalent over the past 8 yr. Although more than 80% became tolerant to these foods by school age, the prevalence of bronchial asthma, atopic dermatitis, allergic rhinitis and allergic conjunctivitis were significantly higher in this group. Moreover, avoidance of other foods (buckwheat, shellfish, fruits and others) at school age was seen at much higher frequencies than in non-food avoiders in infancy (adjusted odds ratio, 7.7; confidence interval, 5.9–10.2). This risk did not differ significantly between those who did and did not develop tolerance to eggs, milk and wheat by 3 yr old. In conclusion, food avoiders in infancy appear to have a higher risk of not only other allergic diseases (‘atopic march’) but also allergy to other foods (‘food allergen march’) at school age, indicating the need for continuous attention to food allergy. [ABSTRACT FROM AUTHOR]
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- 2009
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8. Obesity and the prevalence of allergic diseases in schoolchildren.
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Kusunoki, Takashi, Morimoto, Takeshi, Nishikomori, Ryuta, Heike, Toshio, Ito, Masatoshi, Hosoi, Susumu, and Nakahata, Tatsutoshi
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OBESITY , *ALLERGIES , *CHILDREN'S health , *ASTHMA , *MEDICAL care - Abstract
Although the association between obesity and bronchial asthma (BA) has been gaining more attention, few studies have been conducted concerning the relationship between obesity and other allergic diseases. The objective of this study was to determine whether and how childhood obesity is associated with allergic diseases other than BA, such as atopic dermatitis (AD), allergic rhinitis (AR), allergic conjunctivitis (AC), and either AR or AC (AR/AC). A questionnaire was administered to the parents of 50,086 Japanese schoolchildren. Associations between childhood obesity and the various allergic diseases were evaluated by univariate and multivariate logistic models. Significant associations were found between higher body mass index (BMI) and AD (p = 0.03), and lower BMI and AC (p < 0.0001), and AR/AC (p < 0.0001). There was a significantly higher prevalence of BA in girls with obesity (p = 0.009) than in those without obesity. Significantly lower prevalence of AC (p = 0.01) and AR/AC (p = 0.002) among children with obesity, and AR (p = 0.04) and AR/AC (p = 0.0004) among boys with obesity were observed than those without obesity. Those who were obese and had AD were significantly more likely to have severe symptoms (p = 0.01). Overall, childhood obesity has positive associations with BA prevalence and AD severity, whereas it has negative associations with AR and AC prevalence, especially among boys. Changes in the immunologic balance accompanied by obesity might have different effects on each type of allergic disease. Exploring the mechanisms by which childhood obesity affects allergic status should lead to new management options for childhood allergy. [ABSTRACT FROM AUTHOR]
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- 2008
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9. Fruit intake reduces the onset of respiratory allergic symptoms in schoolchildren.
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Kusunoki, Takashi, Takeuchi, Jiro, Morimoto, Takeshi, Sakuma, Mio, Yasumi, Takahiro, Nishikomori, Ryuta, Higashi, Akane, and Heike, Toshio
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FRUIT , *FOOD consumption , *RESPIRATORY allergy , *SCHOOL children , *EPIDEMIOLOGY - Abstract
Background Previous studies have shown that dietary pattern is associated with allergy prevention. Methods We conducted a prospective cohort study on all primary schools in Omihachiman City, Shiga Prefecture, Japan. Questionnaires regarding allergic symptoms and diet were distributed to the parents of all 759 7-year-old schoolchildren for 4 consecutive years, from 2011 to 2014. Specific immunoglobulin E to inhalant allergens was measured at 10 years of age. Participants were then categorized as low, medium, or high intake during the study period for four food groups (fruits, vegetables, fish, and beans). Logistic regression analysis was performed to estimate odds ratios and 95% confidence intervals. Results A total of 520 children (68.5%) whose parents responded to the questionnaires all 4 years were included in the analysis. The prevalence of asthma, rhinitis, and any allergic symptoms at age 10 was significantly decreased with increases in fruit intake. In addition, the onset of any allergic symptoms during the study period was significantly decreased with increases in fruit intake (33.3%, 28.3%, and 14.3% in children with low, medium, and high fruit intake, respectively; P for trend =.01). The sensitization rate to ragweed at age 10 was significantly decreased with increases in fruit intake ( P for trend =.046). No significant effect was observed for the other three food groups, except for the association between fish intake and new-onset asthma symptoms. Conclusions These findings suggest that higher intake of fruit can help prevent respiratory allergic symptoms in schoolchildren. [ABSTRACT FROM AUTHOR]
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- 2017
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10. Identification of a High-Frequency Somatic NLRC4 Mutation as a Cause of Autoinflammation by Pluripotent Cell-Based Phenotype Dissection.
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Kawasaki, Yuri, Oda, Hirotsugu, Ito, Jun, Niwa, Akira, Tanaka, Takayuki, Hijikata, Atsushi, Seki, Ryosuke, Nagahashi, Ayako, Osawa, Mitsujiro, Asaka, Isao, Watanabe, Akira, Nishimata, Shigeo, Shirai, Tsuyoshi, Kawashima, Hisashi, Ohara, Osamu, Nakahata, Tatsutoshi, Nishikomori, Ryuta, Heike, Toshio, and Saito, Megumu K.
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GENETIC disorder diagnosis , *INFLAMMATION , *CYTOKINES , *DNA , *GENETIC techniques , *HEMATOPOIETIC stem cells , *INTERLEUKIN-1 , *MACROPHAGES , *GENETIC mutation , *PARENTS , *RESEARCH funding , *RNA , *T-test (Statistics) , *TUMOR necrosis factors , *PHENOTYPES , *DESCRIPTIVE statistics , *DIAGNOSIS - Abstract
Objective To elucidate the genetic background of a patient with neonatal-onset multisystem inflammatory disease (NOMID) with no NLRP3 mutation. Methods A Japanese male child diagnosed as having NOMID was studied. The patient did not have any NLRP3 mutation, even as low-frequency mosaicism. We performed whole-exome sequencing on the patient and his parents. Induced pluripotent stem cells (iPSCs) were established from the patient's fibroblasts. The iPSCs were then differentiated into monocyte lineage to evaluate the cytokine profile. Results We established multiple iPSC clones from a patient with NOMID and incidentally found that the phenotypes of monocytes from iPSC clones were heterogeneous and could be grouped into disease and normal phenotypes. Because each iPSC clone was derived from a single somatic cell, we hypothesized that the patient had somatic mosaicism of an interleukin-1β-related gene. Whole-exome sequencing of both representative iPSC clones and the patient's blood revealed a novel heterozygous NLRC4 mutation, p.T177A (c.529A>G), as a specific mutation in diseased iPSC clones. Knockout of the NLRC4 gene using the clustered regularly interspaced short palindromic repeat/Cas9 system in a mutant iPSC clone abrogated the pathogenic phenotype. Conclusion Our findings indicate that the patient has somatic mosaicism of a novel NLRC4 mutation. To our knowledge, this is the first case showing that somatic mutation of NLRC4 causes autoinflammatory symptoms compatible with NOMID. The present study demonstrates the significance of prospective genetic screening combined with iPSC-based phenotype dissection for individualized diagnoses. [ABSTRACT FROM AUTHOR]
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- 2017
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11. Brief Report: Late-Onset Cryopyrin-Associated Periodic Syndrome Due to Myeloid-Restricted Somatic NLRP3 Mosaicism.
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Mensa‐Vilaro, Anna, Teresa Bosque, María, Magri, Giuliana, Honda, Yoshitaka, Martínez‐Banaclocha, Helios, Casorran‐Berges, Marta, Sintes, Jordi, González‐Roca, Eva, Ruiz‐Ortiz, Estibaliz, Heike, Toshio, Martínez‐Garcia, Juan J., Baroja‐Mazo, Alberto, Cerutti, Andrea, Nishikomori, Ryuta, Yagüe, Jordi, Pelegrín, Pablo, Delgado‐Beltran, Concha, and Aróstegui, Juan I.
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CHROMATOGRAPHIC analysis , *INTERLEUKINS , *MOSAICISM , *GENETIC mutation , *RESEARCH funding , *PHENOTYPES , *SEQUENCE analysis , *IN vitro studies , *CRYOPYRIN-associated periodic syndromes , *CHEMICAL inhibitors - Abstract
Objective Gain-of-function NLRP3 mutations cause cryopyrin-associated periodic syndrome (CAPS), with gene mosaicism playing a relevant role in the pathogenesis. This study was undertaken to characterize the genetic cause underlying late-onset but otherwise typical CAPS. Methods We studied a 64-year-old patient who presented with recurrent episodes of urticaria-like rash, fever, conjunctivitis, and oligoarthritis at age 56 years. DNA was extracted from both unfractionated blood and isolated leukocyte and CD34+ subpopulations. Genetic studies were performed using both the Sanger method of DNA sequencing and next-generation sequencing (NGS) methods. In vitro and ex vivo analyses were performed to determine the consequences that the presence of the variant have in the normal structure or function of the protein of the detected variant. Results NGS analyses revealed the novel p.Gln636Glu NLRP3 variant in unfractionated blood, with an allele frequency (18.4%) compatible with gene mosaicism. Sanger sequence chromatograms revealed a small peak corresponding to the variant allele. Amplicon-based deep sequencing revealed somatic NLRP3 mosaicism restricted to myeloid cells (31.8% in monocytes, 24.6% in neutrophils, and 11.2% in circulating CD34+ common myeloid progenitor cells) and its complete absence in lymphoid cells. Functional analyses confirmed the gain-of-function behavior of the gene variant and hyperactivity of the NLRP3 inflammasome in the patient. Treatment with anakinra resulted in good control of the disease. Conclusion We identified the novel gain-of-function p.Gln636Glu NLRP3 mutation, which was detected as a somatic mutation restricted to myeloid cells, as the cause of late-onset but otherwise typical CAPS. Our results expand the diversity of CAPS toward milder phenotypes than previously reported, including those starting during adulthood. [ABSTRACT FROM AUTHOR]
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- 2016
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12. Clinical and Genetic Features of Patients With TNFRSF1A Variants in Japan: Findings of a Nationwide Survey.
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Ueda, Naoyasu, Ida, Hiroaki, Washio, Masakazu, Miyahara, Hisaaki, Tokunaga, Shoji, Tanaka, Fumiko, Takahashi, Hiroki, Kusuhara, Koichi, Ohmura, Koichiro, Nakayama, Manabu, Ohara, Osamu, Nishikomori, Ryuta, Minota, Seiji, Takei, Shuji, Fujii, Takao, Ishigatsubo, Yoshiaki, Tsukamoto, Hiroshi, Tahira, Tomoko, and Horiuchi, Takahiko
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CHI-squared test , *CONFIDENCE intervals , *FISHER exact test , *FLUORESCENT antibody technique , *GENE expression , *GENETIC polymorphisms , *GENETIC mutation , *POLYMERASE chain reaction , *RESEARCH funding , *SURVEYS , *LOGISTIC regression analysis , *DATA analysis software , *ODDS ratio , *TUMOR necrosis factor receptor-associated periodic syndrome , *GENOTYPES , *SYMPTOMS , *GENETICS - Abstract
Objective To elucidate the clinical and genetic features of patients with TNFRSF1A variants in Japan using data obtained from a nationwide survey conducted by the Ministry of Health, Labor, and Welfare of Japan study group for tumor necrosis factor receptor-associated periodic syndrome (TRAPS). Methods Inquiries were sent to 2,900 departments of internal medicine and pediatrics in all hospitals with more than 200 beds in Japan, asking whether they had patients in whom TRAPS was suspected. Genetic tests for TNFRSF1A, MEFV, and MVK were performed on 169 patients. Cell surface expression of TNFRSF1A variants was assessed using 293T cells. Results Ten patients from 10 independent families were found to have TNFRSF1A variants. We collected clinical and genetic information on 41 additional patients with TNFRSF1A variants and symptoms of inflammation from 23 independent families; 17 of these patients had not been described in the literature. The common clinical features of Japanese patients were fever of >38°C (100% of patients), arthralgia (59%), and rash (55%). The prevalence of abdominal pain (36%), myalgia (43%), and amyloidosis (0%) was significantly lower in Japanese patients than in Caucasian patients. The most common variant was T61I (appearing in 49% of patients), and it was identified in 7 of 363 healthy controls. Defects in cysteine residues and the T50M variant were associated with decreased cell surface expression, while other variants, including T61I, were not. Conclusion Patients with TNFRSF1A variants are very rare in Japan, as in other countries, but there are a number of clinical and genetic differences between Japanese and Caucasian patients. The pathogenic significance of the T61I variant remains unclear. [ABSTRACT FROM AUTHOR]
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- 2016
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13. Diagnostic accuracy of endoscopic features of pediatric acute gastrointestinal graft-versus-host disease.
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Hiejima, Eitaro, Nakase, Hiroshi, Matsuura, Minoru, Honzawa, Yusuke, Higuchi, Hirokazu, Saida, Satoshi, Umeda, Katsutsugu, Hiramatsu, Hidefumi, Adachi, Souichi, Izawa, Kazushi, Kawai, Tomoki, Yasumi, Takahiro, Nishikomori, Ryuta, and Heike, Toshio
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GRAFT versus host disease , *GRAFT versus host reaction , *HEMATOPOIETIC stem cell transplantation , *ENDOSCOPY , *DIAGNOSTIC imaging - Abstract
Background and Aim Acute gastrointestinal graft-versus-host disease (GI-GVHD) is a major cause of morbidity and mortality after hematopoietic stem cell transplantation (HSCT). There are very few studies on specific endoscopic findings in pediatric acute GI-GVHD. The aim of this retrospective case-control study was to elucidate the characteristic endoscopic findings in pediatric acute GI-GVHD that improve the diagnostic accuracy of endoscopy. Methods All consecutive patients under 18 years of age who underwent allogeneic HSCT in Kyoto University Hospital from May 2003 to October 2014 were identified retrospectively. Patients who underwent GI endoscopy as a result of sustained GI symptoms were identified. Intestinal villous patterns were evaluated by magnification endoscopy with the water-immersion technique. The patients were diagnosed with acute GI-GVHD and non-GVHD on the basis of biopsy histology. Endoscopic findings of the two groups were compared. Results Of the 171 patients who underwent HSCT, 30 underwent GI endoscopy. Of these, 17 and nine were diagnosed with acute GI-GVHD and non-GVHD, respectively. Compared with non-GVHD, acute GI-GVHD was associated significantly more often with short blunt villi in the duodenum ( P = 0.013), variable defect villi and short blunt villi in the ileum ( P = 0.009 and 0.035, respectively), and edema, erosion, and tortoiseshell-like mucosae in the colon ( P = 0.017, 0.023, and 0.017, respectively). Conclusion Pediatric acute GI-GVHD was associated with several characteristic features on magnifying endoscopy with the water-immersion technique. These features will be useful for endoscopic diagnosis of pediatric acute GI-GVHD. [ABSTRACT FROM AUTHOR]
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- 2016
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14. Sports activities enhance the prevalence of rhinitis symptoms in schoolchildren.
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Kusunoki, Takashi, Takeuchi, Jiro, Morimoto, Takeshi, Sakuma, Mio, Mukaida, Kumiko, Yasumi, Takahiro, Nishikomori, Ryuta, and Heike, Toshio
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RHINITIS , *SPORTS physiology , *DISEASE prevalence , *ECZEMA , *ALLERGIES , *IMMUNOGLOBULIN E , *HEALTH of school children - Abstract
Background To evaluate the association between sports activities and allergic symptoms, especially rhinitis, among schoolchildren. Methods This longitudinal survey of schoolchildren collected data from questionnaires regarding allergic symptoms based on the International Study of Asthma and Allergies in Childhood ( ISAAC) program and sports participation that were distributed to the parents of children at all 12 public primary schools in Ohmi-Hachiman City, Shiga Prefecture, Japan. Data were collected annually from 2011 until 2014, when the children reached 10 years of age. Blood samples were obtained in 2014, and the levels of immunoglobulin (Ig)E specific to four inhalant allergens were measured. Results Data from 558 children were analyzed. At 10 years of age, prevalence of asthma and eczema did not differ significantly, while rhinitis was significantly higher (p = 0.009) among children who participated in sports. Prevalence of rhinitis increased as the frequency or duration of sports participation increased (p < 0.01). The prevalence of new-onset rhinitis increased significantly among 10-year-olds with increasing duration of participation in sports (p = 0.03). Among those who participated in continuous sports activities, the prevalence of rhinitis was significantly higher with prolonged eczema (p = 0.006). Sports activities did not increase sensitization to inhalant allergens. Conclusion Sports activities enhance the prevalence of rhinitis in schoolchildren. Prolonged eczema, together with sports participation, further promotes the symptoms. The mechanisms of these novel findings warrant further investigation. [ABSTRACT FROM AUTHOR]
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- 2016
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15. Laboratory parameters identify familial haemophagocytic lymphohistiocytosis from other forms of paediatric haemophagocytosis.
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Yasumi, Takahiro, Hori, Masayuki, Hiejima, Eitaro, Shibata, Hirofumi, Izawa, Kazushi, Oda, Hirotsugu, Yoshioka, Kouhei, Nakagawa, Kenji, Kawai, Tomoki, Nishikomori, Ryuta, Ohara, Osamu, and Heike, Toshio
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LYMPHOMAS in children , *IMMUNOLOGIC diseases , *INTERLEUKIN-2 receptors , *LYMPHOCYTES , *PATHOLOGICAL physiology - Abstract
Haemophagocytic lymphohistiocytosis ( HLH) is a life-threatening syndrome of immune dysregulation and is classified as primary or secondary according to the underlying aetiology. The treatment strategies recommended for these two groups differ substantially; however, it is thought to be impossible to predict the underlying causes of HLH using conventional laboratory tests. Recent studies show that serum levels of soluble interleukin-2 receptor ( sIL2R) and ferritin are useful for differentiating some forms of HLH. The present study reports that combinations of common laboratory parameters, such as the percentage of total lymphocytes within the peripheral blood leucocyte population, serum levels of lactate dehydrogenase and the sIL2R/ferritin ratio, are useful for identifying patients with familial haemophagocytic lymphohistiocytosis and for differentiating the underlying aetiology of paediatric HLH during the early course of the disease. These findings suggest that the pathogenesis of HLH differs greatly in terms of innate and adaptive immunity depending on the aetiology and may provide a new approach to unravelling the complex pathophysiology underlying this syndrome. [ABSTRACT FROM AUTHOR]
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- 2015
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16. Enhanced Chondrogenesis of Induced Pluripotent Stem Cells From Patients With Neonatal-Onset Multisystem Inflammatory Disease Occurs via the Caspase 1-Independent cAMP/Protein Kinase A/CREB Pathway.
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Yokoyama, Koji, Ikeya, Makoto, Umeda, Katsutsugu, Oda, Hirotsugu, Nodomi, Seishiro, Nasu, Akira, Matsumoto, Yoshihisa, Izawa, Kazushi, Horigome, Kazuhiko, Kusaka, Toshimasa, Tanaka, Takayuki, Saito, Megumu K., Yasumi, Takahiro, Nishikomori, Ryuta, Ohara, Osamu, Nakayama, Naoki, Nakahata, Tatsutoshi, Heike, Toshio, and Toguchida, Junya
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ACADEMIC medical centers , *CHONDROGENESIS , *ENZYME-linked immunosorbent assay , *IMMUNOBLOTTING , *RESEARCH funding , *STEM cells , *T-test (Statistics) , *GENE expression profiling , *CRYOPYRIN-associated periodic syndromes - Abstract
Objective Neonatal-onset multisystem inflammatory disease (NOMID) is a dominantly inherited autoinflammatory disease caused by NLRP3 mutations. NOMID pathophysiology is explained by the NLRP3 inflammasome, which produces interleukin-1β (IL-1β). However, epiphyseal overgrowth in NOMID is resistant to anti-IL-1 therapy and may therefore occur independently of the NLRP3 inflammasome. This study was undertaken to investigate the effect of mutated NLRP3 on chondrocytes using induced pluripotent stem cells (iPSCs) from patients with NOMID. Methods We established isogenic iPSCs with wild-type or mutant NLRP3 from 2 NOMID patients with NLRP3 somatic mosaicism. The iPSCs were differentiated into chondrocytes in vitro and in vivo. The phenotypes of chondrocytes with wild-type and mutant NLRP3 were compared, particularly the size of the chondrocyte tissue produced. Results Mutant iPSCs produced larger chondrocyte masses than wild-type iPSCs owing to glycosaminoglycan overproduction, which correlated with increased expression of the chondrocyte master regulator SOX9. In addition, in vivo transplantation of mutant cartilaginous pellets into immunodeficient mice caused disorganized endochondral ossification. Enhanced chondrogenesis was independent of caspase 1 and IL-1, and thus the NLRP3 inflammasome. Investigation of the human SOX9 promoter in chondroprogenitor cells revealed that the CREB/ATF-binding site was critical for SOX9 overexpression caused by mutated NLRP3. This was supported by increased levels of cAMP and phosphorylated CREB in mutant chondroprogenitor cells. Conclusion Our findings indicate that the intrinsic hyperplastic capacity of NOMID chondrocytes is dependent on the cAMP/PKA/CREB pathway, independent of the NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]
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- 2015
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17. Effect of eczema on the association between season of birth and food allergy in Japanese children.
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Kusunoki, Takashi, Morimoto, Takeshi, Sakuma, Mio, Mukaida, Kumiko, Yasumi, Takahiro, Nishikomori, Ryuta, and Heike, Toshio
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CHI-squared test , *CHILDBIRTH , *ECZEMA , *FOOD allergy in children , *QUESTIONNAIRES , *RESEARCH funding , *SEASONS , *T-test (Statistics) , *LOGISTIC regression analysis , *DATA analysis software - Abstract
Background Food allergy ( FA) in childhood has been shown to be more prevalent in those born in autumn and winter. The mechanisms of this season-of-birth effect remain unclear, although shortage of vitamin D during infancy has been considered one possible mechanism. The purpose of this study was to investigate the effect of eczema on the season-of-birth effect on FA in infancy. Methods A questionnaire survey on the prevalence of allergic diseases was completed by the parents of 14 669 Japanese schoolchildren, aged 7-15 years, in Kyoto City, Japan. Logistic regression models were constructed to compare the prevalence of FA in infancy according to season of birth. Results Those born in autumn and winter had a significantly higher prevalence of FA in infancy compared to those born in spring and summer in a multivariate model (4.8% vs 3.6%, P = 0.001). The difference, however, was no longer significant when eczema before 6 months was included as either an additional or only confounding factor. The difference among those with and without eczema before 6 months was further analyzed, and it was found that, in both groups, there was no difference between those born in spring and summer and those born in autumn and winter. Conclusions The season-of-birth effect on FA in infancy was significantly affected by the existence of eczema before 6 months in Japanese children. Eczema before 6 months may be the factor directly related to the season-of-birth effect on FA in infancy. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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18. Birth order effect on childhood food allergy.
- Author
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Kusunoki, Takashi, Mukaida, Kumiko, Morimoto, Takeshi, Sakuma, Mio, Yasumi, Takahiro, Nishikomori, Ryuta, and Heike, Toshio
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BIRTH order , *FOOD allergy in children , *ATOPIC dermatitis , *ALLERGIC rhinitis , *ALLERGIES - Abstract
To cite this article: Kusunoki T, Mukaida K, Morimoto T, Sakuma M, Yasumi T, Nishikomori R, Heike T. Birth order effect on childhood food allergy. Pediatric Allergy Immunology 2012: 23: 250-254. Abstract Higher birth order is associated with a smaller risk of allergy (birth order effect). The purpose of this study was to compare the significance of the birth order effect on the prevalence of specific allergic diseases [bronchial asthma (BA), atopic dermatitis (AD), allergic rhinitis (AR), allergic conjunctivitis (AC), and food allergy (FA)] among schoolchildren. A questionnaire survey dealing with the prevalence of allergic diseases was administered to the parents of 14,669 schoolchildren aged 7-15 yr. Based on the data, the prevalence of each allergic disease was compared according to birth order (1st, 2nd, and 3rd or later). Multiple regression analysis was performed to test the significance of the differences. There was no significant difference in the prevalence of BA or AD according to birth order. The prevalence of AR, AC, and FA decreased significantly as birth order increased. The prevalence of FA among those with 1st, 2nd, and 3rd or later birth order was 4.0%, 3.4%, and 2.6%, respectively (p = 0.01). With respect to symptoms in infancy, the prevalence of wheeze increased significantly and that of FA and eczema in infancy decreased significantly as birth order increased. The present data show a significant birth order effect on FA. The effect was also observed for the prevalence of FA and eczema in infancy. These data support the concept of early, non-allergen-specific programming of IgE-mediated immunity. [ABSTRACT FROM AUTHOR]
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- 2012
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19. Acute liver failure in young children with systemic-onset juvenile idiopathic arthritis without macrophage activation syndrome: Report of two cases.
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Hiejima, Eitaro, Komatsu, Haruki, Takeda, Yoshitake, Sogo, Tsuyoshi, Inui, Ayano, Okafuji, Ikuo, Nishikomori, Ryuta, Nakahata, Tatsutoshi, and Fujisawa, Tomoo
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LIVER failure , *BLOOD plasma , *BLOOD transfusion , *IMMUNOSUPPRESSIVE agents , *CYCLOSPORINE - Abstract
Acute liver failure (ALF) with macrophage activation syndrome (MAS) is well known as a complication of systemic-onset juvenile idiopathic arthritis (S-JIA). However, liver failure without overt MAS is rare in S-JIA. We encountered two Japanese children with S-JIA in whom ALF developed during the remission of clinical manifestations. ALF without MAS was improved with plasma exchange and cyclosporine A combined with pulse methylprednisolone. [ABSTRACT FROM AUTHOR]
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- 2012
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20. Enhanced NF-[kappa]B activation with an inflammasome activator correlates with activity of autoinflammatory disease associated with NLRP3 mutations outside of exon 3: comment on the article by Jéru et al.
- Author
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Kambe, Naotomo, Satoh, Takashi, Tanizaki, Hideaki, Fujisawa, Akihiro, Saito, Megumu K, and Nishikomori, Ryuta
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- 2010
21. Enhanced NF-κB activation with an inflammasome activator correlates with activity of autoinflammatory disease associated with NLRP3 mutations outside of exon 3: comment on the article by Jéru et al.
- Author
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Kambe, Naotomo, Satoh, Takashi, Tanizaki, Hideaki, Fujisawa, Akihiro, Saito, Megumu K., Nishikomori, Ryuta, Jéru, Isabelle, and Amselem, Serge
- Abstract
Two letters to the editor are presented in response to the article "Functional Consequences of a Germline Mutation in Theleucin-Rich Repeat domain of NLRP3 Identified in an Atypical Autoinflammatory Disorder," by I. Jeru and colleagues, published in the 2010 issue of "Arthritis and Rheumatology," including a comment on the article and the authors' reply.
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- 2010
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22. Tumor in chest wall caused by Mycobacterium bovis BCG infection: Identification on polymerase chain reaction of formalin-fixed specimen.
- Author
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Yokoyama, Koji, Kubo, Kenji, Yoshida, Shiomi, Wada, Takayuki, Kawai, Tomoki, Nishikomori, Ryuta, and Yoshida, Akira
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ANTI-infective agents , *MYCOBACTERIAL disease diagnosis , *BCG vaccines , *CHEST X rays , *HISTOLOGICAL techniques , *IMMUNE response , *IMMUNOCOMPETENT cells , *MAGNETIC resonance imaging , *MYCOBACTERIAL diseases , *POLYMERASE chain reaction , *RADIONUCLIDE imaging , *GENETIC testing , *DISEASE complications , *CHILDREN ,CHEST tumors - Abstract
A case study of a 3-year-old Japanese boy presented with a tumor in the anterior chest wall is presented. Topics discussed include examinations revealing subcutaneous lobular cystic lesion on the precordium; a granulomatous infiltrate with central caseation necrosis indicated by histopathology; and treatment with isoniazid, rifampicin and pyrazinamide.
- Published
- 2016
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